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The map label for this gene is cobS [H]

Identifier: 218709690

GI number: 218709690

Start: 1826849

End: 1827655

Strand: Reverse

Name: cobS [H]

Synonym: VS_1701

Alternate gene names: 218709690

Gene position: 1827655-1826849 (Counterclockwise)

Preceding gene: 218709691

Following gene: 218709689

Centisome position: 55.4

GC content: 43.62

Gene sequence:

>807_bases
ATGAGTGATGCACCAAAAAGATCGTTGAAAGATAGCATCACATACCAATGGGAACTTTTTTCGTTGGCGATGGGTTTCTT
TTCTCGTTTGCCGATGCCGAAGAATACGCCTTATTCAGAAGAGCGCATGAATCGTTCGGGTCGCTACTTTTCAACGGTTG
GTTTGTTACTTGGTGTTCTGTGTGGTGGTGTGTTCTTGCTACTCGATGCAATACTGCCGAGTGCAGTGGCCATCTTTTTG
ATGATGAGCTTTAGCTTGATGCTCACAGGTGCATTCCATGAAGACGGCTTAACCGATATGGCGGATGGTATTGGCGGCGG
TATGACCTTAGAACGCCGTTTGACCATCATGAAAGACAGTCGAATTGGTACTTATGGCGCGTCTGCACTGGTTATGGCTC
TGCTTGGCAAGTGGGTTCTATTAAACGAACTAGCGAATATGACAAATTTGTTTATGGCTATCGTGACCAGTTATACGTTT
AGCCGCGCGATTGCCGCATCACTTATTTTTGATATGCCTTATGTCAGTGATTTAGATACCAGTAAAAGTAAGCCATTGGC
GAACAAGCAAACTAAAGGTGAACTTGTCTTTTTATTGTTTGTAGGTGTGTTGCCTAGCCTGTGGTTTGGTCTTGAGCTCG
CTTTGGTTTTATCTTTCTTCGCCTATCTGTTCAGAGTAGGTTTCAAAAAATGGTTAACGGCTCGAATTGGTGGCTTTACT
GGCGACTGTTTGGGTGCGGCTCAACAGTTGATGGAGTTACTGATTTACCTTGTGTTCATTACTGCTTTTTACAACGGTTT
TTTATAA

Upstream 100 bases:

>100_bases
GCGAGGGCACAGGTGCTGCGCTAGCTATGCCAATAATTCGTGCGGCGGCAGAGTTCTATAACAACATGGCGAGTTTTGCG
AGTGCTGGAGTCACGGTTTA

Downstream 100 bases:

>100_bases
CAGGTTCCTATGACAAACACGAATCAAGCTAATCAAATTAATCAAGTAAGTCAGCGAAATAGCCATCTGGTATTGGGTGG
GGCTCGTTCTGGTAAGTCTA

Product: cobalamin (5'-phosphate) synthase

Products: GMP; adenosylcobalamin

Alternate protein names: NA

Number of amino acids: Translated: 268; Mature: 267

Protein sequence:

>268_residues
MSDAPKRSLKDSITYQWELFSLAMGFFSRLPMPKNTPYSEERMNRSGRYFSTVGLLLGVLCGGVFLLLDAILPSAVAIFL
MMSFSLMLTGAFHEDGLTDMADGIGGGMTLERRLTIMKDSRIGTYGASALVMALLGKWVLLNELANMTNLFMAIVTSYTF
SRAIAASLIFDMPYVSDLDTSKSKPLANKQTKGELVFLLFVGVLPSLWFGLELALVLSFFAYLFRVGFKKWLTARIGGFT
GDCLGAAQQLMELLIYLVFITAFYNGFL

Sequences:

>Translated_268_residues
MSDAPKRSLKDSITYQWELFSLAMGFFSRLPMPKNTPYSEERMNRSGRYFSTVGLLLGVLCGGVFLLLDAILPSAVAIFL
MMSFSLMLTGAFHEDGLTDMADGIGGGMTLERRLTIMKDSRIGTYGASALVMALLGKWVLLNELANMTNLFMAIVTSYTF
SRAIAASLIFDMPYVSDLDTSKSKPLANKQTKGELVFLLFVGVLPSLWFGLELALVLSFFAYLFRVGFKKWLTARIGGFT
GDCLGAAQQLMELLIYLVFITAFYNGFL
>Mature_267_residues
SDAPKRSLKDSITYQWELFSLAMGFFSRLPMPKNTPYSEERMNRSGRYFSTVGLLLGVLCGGVFLLLDAILPSAVAIFLM
MSFSLMLTGAFHEDGLTDMADGIGGGMTLERRLTIMKDSRIGTYGASALVMALLGKWVLLNELANMTNLFMAIVTSYTFS
RAIAASLIFDMPYVSDLDTSKSKPLANKQTKGELVFLLFVGVLPSLWFGLELALVLSFFAYLFRVGFKKWLTARIGGFTG
DCLGAAQQLMELLIYLVFITAFYNGFL

Specific function: Joins Ado-cobinamide-GDP and alpha-ribazole to generate adenosylcobalamin (Ado-cobalamin) [H]

COG id: COG0368

COG function: function code H; Cobalamin-5-phosphate synthase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CobS family [H]

Homologues:

Organism=Escherichia coli, GI1788301, Length=251, Percent_Identity=33.0677290836653, Blast_Score=100, Evalue=1e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003805 [H]

Pfam domain/function: PF02654 CobS [H]

EC number: 2.7.8.26

Molecular weight: Translated: 29643; Mature: 29511

Theoretical pI: Translated: 8.62; Mature: 8.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
5.6 %Met     (Translated Protein)
6.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
5.2 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SDAPKRSLKDSITYQWELFSLAMGFFSRLPMPKNTPYSEERMNRSGRYFSTVGLLLGVL
CCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHCCCCCHHHHHHHHHHHH
CGGVFLLLDAILPSAVAIFLMMSFSLMLTGAFHEDGLTDMADGIGGGMTLERRLTIMKDS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHCCHHHHHHCCCCCCCHHHHHHHHHCC
RIGTYGASALVMALLGKWVLLNELANMTNLFMAIVTSYTFSRAIAASLIFDMPYVSDLDT
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
SKSKPLANKQTKGELVFLLFVGVLPSLWFGLELALVLSFFAYLFRVGFKKWLTARIGGFT
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
GDCLGAAQQLMELLIYLVFITAFYNGFL
HHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: adenosylcobinamide-GDP; alpha-ribazole

Specific reaction: adenosylcobinamide-GDP + alpha-ribazole = GMP + adenosylcobalamin

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12620739 [H]