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The map label for this gene is hgiDIIM [H]

Identifier: 218709639

GI number: 218709639

Start: 1781113

End: 1782180

Strand: Reverse

Name: hgiDIIM [H]

Synonym: VS_1649

Alternate gene names: 218709639

Gene position: 1782180-1781113 (Counterclockwise)

Preceding gene: 218709640

Following gene: 218709638

Centisome position: 54.02

GC content: 43.82

Gene sequence:

>1068_bases
ATGGTAGTTGAAGTGAAGATCGTAGGTGTAGACCTTTTTTGCGGTGCAGGTGGCTTGACACACGGTTTGCAACAGGCCGG
AATTGACGTAAGAGCAGGGTTTGATCTTGAGTCAACGTGCAAATTCCCTTATGAGCATAATAATCAAGCTGAATTTGTAC
AGAAAAGTGTATCTGACTTAACGGGAAAAGATATTGTCTCTAGATTCGATGAACATGCGTACACGTTACTGGCTGGTTGT
GCTCCGTGCCAACCGTTCTCTAAGTACACACAGGCAAAGCCTCAAGACGACAGATGGGGGCTCCTGTATCAATTTGCTCG
GTTGGTTCAAGAATCTAAACCAGATTTTATTACCATGGAAAATGTTCCAGACTTAGATAAGCATAAAGTCTACTCAGATT
TTAAAGAGGGTCTTATGGCCGAGGGCTATTACGTTGATGAGCAAGTGGTTTTTTGCCCGGATTTCGGAATGGCACAGACT
CGTAAACGATTAGTATTATTAGCTTCACGTTTAGGCGACATAAAATTGATCAAGCCTACTCACAGTAAATGCCAGTATGT
AACGGTTAAGGAAGTTATTGGGCACTTGCCTAGATTGAGAGCGGGTTGTGCAAACAAGAATGATCCTTTACATCGTTCTA
GTAGCCTATCTGAGTTAAATATGAAGAGAATAAAGGCATCAAAACCAGGCGGTACATGGCGTGACTGGCCAGAAGAACTT
AGAGCAAAATGTCATACGAAGGATAGTGGTAAAGGATATGCGAGTGTTTATGGCCGAATGTCATGGAATGAGCCCAGCCC
TACAATGACGACACAGTGTTACGGCTTTGGAAATGGGCGTTTTGGGCACCCGTCACAAGCTAGGGCGATTTCTTTGAGAG
AAGCGGCTATCCTGCAGTCTTTTCCAGACCACTATGAATTTGTGGCACCCGGTGGTAGCTACGAGACGAAACATTTAGGC
AAAATGATCGGAAATGCAGTGCCAGTTCGATTAGGAAAGATTGTTGGGGACTCGCTCATAAATCACGCAAAAGAATTGAG
CCTCTTTCAGAAGTCTAATTCCAAATGA

Upstream 100 bases:

>100_bases
ATTCTGAGTAGGTGATAGAGCGAATTAAATCTGCTTCCTTATATTCGGGCTCGATGTCTTACCAAAACAATGGTAGAATA
TGTACATAGTTAAAATTTAA

Downstream 100 bases:

>100_bases
GTTTTGATTAATAAATTTGTGGCCTTGATTATCAATATGAAACTTATTGTGTCTTAAGTCCTAAGTTTTTGTTCCAACAC
ATTGAATTAAAAGGTGTGTA

Product: cytosine-specific methyltransferase HgiDII; restriction system

Products: NA

Alternate protein names: M.HgiDII; Cytosine-specific methyltransferase HgiDII [H]

Number of amino acids: Translated: 355; Mature: 355

Protein sequence:

>355_residues
MVVEVKIVGVDLFCGAGGLTHGLQQAGIDVRAGFDLESTCKFPYEHNNQAEFVQKSVSDLTGKDIVSRFDEHAYTLLAGC
APCQPFSKYTQAKPQDDRWGLLYQFARLVQESKPDFITMENVPDLDKHKVYSDFKEGLMAEGYYVDEQVVFCPDFGMAQT
RKRLVLLASRLGDIKLIKPTHSKCQYVTVKEVIGHLPRLRAGCANKNDPLHRSSSLSELNMKRIKASKPGGTWRDWPEEL
RAKCHTKDSGKGYASVYGRMSWNEPSPTMTTQCYGFGNGRFGHPSQARAISLREAAILQSFPDHYEFVAPGGSYETKHLG
KMIGNAVPVRLGKIVGDSLINHAKELSLFQKSNSK

Sequences:

>Translated_355_residues
MVVEVKIVGVDLFCGAGGLTHGLQQAGIDVRAGFDLESTCKFPYEHNNQAEFVQKSVSDLTGKDIVSRFDEHAYTLLAGC
APCQPFSKYTQAKPQDDRWGLLYQFARLVQESKPDFITMENVPDLDKHKVYSDFKEGLMAEGYYVDEQVVFCPDFGMAQT
RKRLVLLASRLGDIKLIKPTHSKCQYVTVKEVIGHLPRLRAGCANKNDPLHRSSSLSELNMKRIKASKPGGTWRDWPEEL
RAKCHTKDSGKGYASVYGRMSWNEPSPTMTTQCYGFGNGRFGHPSQARAISLREAAILQSFPDHYEFVAPGGSYETKHLG
KMIGNAVPVRLGKIVGDSLINHAKELSLFQKSNSK
>Mature_355_residues
MVVEVKIVGVDLFCGAGGLTHGLQQAGIDVRAGFDLESTCKFPYEHNNQAEFVQKSVSDLTGKDIVSRFDEHAYTLLAGC
APCQPFSKYTQAKPQDDRWGLLYQFARLVQESKPDFITMENVPDLDKHKVYSDFKEGLMAEGYYVDEQVVFCPDFGMAQT
RKRLVLLASRLGDIKLIKPTHSKCQYVTVKEVIGHLPRLRAGCANKNDPLHRSSSLSELNMKRIKASKPGGTWRDWPEEL
RAKCHTKDSGKGYASVYGRMSWNEPSPTMTTQCYGFGNGRFGHPSQARAISLREAAILQSFPDHYEFVAPGGSYETKHLG
KMIGNAVPVRLGKIVGDSLINHAKELSLFQKSNSK

Specific function: This methylase recognizes the double-stranded sequence GTCGAC, causes specific methylation on C-? on both strands and protects the DNA from cleavage by the HgiDII endonuclease [H]

COG id: COG0270

COG function: function code L; Site-specific DNA methylase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the C5-methyltransferase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018117
- InterPro:   IPR001525 [H]

Pfam domain/function: PF00145 DNA_methylase [H]

EC number: =2.1.1.37 [H]

Molecular weight: Translated: 39572; Mature: 39572

Theoretical pI: Translated: 8.74; Mature: 8.74

Prosite motif: PS00095 C5_MTASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVVEVKIVGVDLFCGAGGLTHGLQQAGIDVRAGFDLESTCKFPYEHNNQAEFVQKSVSDL
CEEEEEEEEEEEEECCCHHHHHHHHCCCEEEECCCHHHCCCCCCCCCCHHHHHHHHHHHC
TGKDIVSRFDEHAYTLLAGCAPCQPFSKYTQAKPQDDRWGLLYQFARLVQESKPDFITME
CHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCEEEEC
NVPDLDKHKVYSDFKEGLMAEGYYVDEQVVFCPDFGMAQTRKRLVLLASRLGDIKLIKPT
CCCCCHHHHHHHHHHHHHHHCCEEECCEEEECCCCCHHHHHHHHHHHHHHCCCEEEECCC
HSKCQYVTVKEVIGHLPRLRAGCANKNDPLHRSSSLSELNMKRIKASKPGGTWRDWPEEL
CCCCEEEEHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCHHHH
RAKCHTKDSGKGYASVYGRMSWNEPSPTMTTQCYGFGNGRFGHPSQARAISLREAAILQS
HHHHCCCCCCCCEEHHEECCCCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHH
FPDHYEFVAPGGSYETKHLGKMIGNAVPVRLGKIVGDSLINHAKELSLFQKSNSK
CCCCCEEECCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MVVEVKIVGVDLFCGAGGLTHGLQQAGIDVRAGFDLESTCKFPYEHNNQAEFVQKSVSDL
CEEEEEEEEEEEEECCCHHHHHHHHCCCEEEECCCHHHCCCCCCCCCCHHHHHHHHHHHC
TGKDIVSRFDEHAYTLLAGCAPCQPFSKYTQAKPQDDRWGLLYQFARLVQESKPDFITME
CHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCEEEEC
NVPDLDKHKVYSDFKEGLMAEGYYVDEQVVFCPDFGMAQTRKRLVLLASRLGDIKLIKPT
CCCCCHHHHHHHHHHHHHHHCCEEECCEEEECCCCCHHHHHHHHHHHHHHCCCEEEECCC
HSKCQYVTVKEVIGHLPRLRAGCANKNDPLHRSSSLSELNMKRIKASKPGGTWRDWPEEL
CCCCEEEEHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCHHHH
RAKCHTKDSGKGYASVYGRMSWNEPSPTMTTQCYGFGNGRFGHPSQARAISLREAAILQS
HHHHCCCCCCCCEEHHEECCCCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHH
FPDHYEFVAPGGSYETKHLGKMIGNAVPVRLGKIVGDSLINHAKELSLFQKSNSK
CCCCCEEECCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1937045; 7607523 [H]