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The map label for this gene is leuO [H]

Identifier: 218709630

GI number: 218709630

Start: 1761425

End: 1762339

Strand: Reverse

Name: leuO [H]

Synonym: VS_1640

Alternate gene names: 218709630

Gene position: 1762339-1761425 (Counterclockwise)

Preceding gene: 218709631

Following gene: 218709628

Centisome position: 53.42

GC content: 41.97

Gene sequence:

>915_bases
ATGAAAAACACTGATTTTAGTCTGATCCCGTATTTTGTTGTCATGATGGAAGAGCTCTCTATCTCGAATACCGCGAAACG
TTTTGGCGTTACTCAGCCCGCAATTAGTAAGGCGCTTAACCGACTAAAAGAAACCTATGATGACCCGTTGTTTGTGAAAA
CAAAGGAAGGTTATAGACCGAGCAGTTTTTGTTATGACATCTACCCTCAGATAAAACAGGCCTACGATGCCTTTGAATCG
ACTTTCCCGAGTAAGCGAAAGTTCGACCCAACAAAAATTGAAAGGTCGTTTAATGTCGCTTGCATTAGTGGTGCAACTTA
TTCATTGATGAAGCGTGTATCCGATATCCTCTATGCGGAAACGCCAGATATCGGTCTTCATATTGACCCTTACTATACTG
CAGACATCATTCTAGACCTTCGCCAACACCGATATGATTTGTGTATAGTCCATACGCCTATCAACGATCCGGCAATGGAA
TGTATTCAGCTCTCTAACGAACCTATGGTGGTTGCTTATGATAAAAACCACCCTAGATTAGGGAACGAGATTACGTTGGA
AGAATACCTTGAAGAGAAGCATGTTCTTCATAGTATGTGGGATACCAAAGAATCGCCGTTAACCAAAACCAGTAAGTATG
CGAAGAGTAGAAAGGTTGCACGAAAGGCGCCTGGCCCATTTGAAATGCTTGATATGGTAAGAGGAACGGATTGGCTTTGT
ATTAGTCAGAAATCGATACTTGAAAGGTATGGCCTGTTGGATGATTTTAAGCTGTCGCCTTTACCGTTTGAATCGGATCT
TGGTACTGAATATCTGGTGTGGCACAAGAGTCGTAATGCAGATATGGCGCATTCATGGTTTAGAAAAAAGATTGTTGATG
CCTCACAAAAAAAGCCCCAATGGGGCTTTAATTAG

Upstream 100 bases:

>100_bases
ACTTCCCAAAACAAAAGGGTCGCTAATGAAGTGCTTGGTTGATAAGGTATAATTCAATGATATGAAAGTTAATTTAGTAC
GCGAGTTTGGTAAATAGAAC

Downstream 100 bases:

>100_bases
TCAGTTATCTTTAATGGTCGGTAGCCAGGTTATCTAGCTGTTGCTTCGCTGTTTAACCCTTGCTCCGCTATTTCGCGTTT
TGTGCTTGCATCTGCTGAAT

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 304; Mature: 304

Protein sequence:

>304_residues
MKNTDFSLIPYFVVMMEELSISNTAKRFGVTQPAISKALNRLKETYDDPLFVKTKEGYRPSSFCYDIYPQIKQAYDAFES
TFPSKRKFDPTKIERSFNVACISGATYSLMKRVSDILYAETPDIGLHIDPYYTADIILDLRQHRYDLCIVHTPINDPAME
CIQLSNEPMVVAYDKNHPRLGNEITLEEYLEEKHVLHSMWDTKESPLTKTSKYAKSRKVARKAPGPFEMLDMVRGTDWLC
ISQKSILERYGLLDDFKLSPLPFESDLGTEYLVWHKSRNADMAHSWFRKKIVDASQKKPQWGFN

Sequences:

>Translated_304_residues
MKNTDFSLIPYFVVMMEELSISNTAKRFGVTQPAISKALNRLKETYDDPLFVKTKEGYRPSSFCYDIYPQIKQAYDAFES
TFPSKRKFDPTKIERSFNVACISGATYSLMKRVSDILYAETPDIGLHIDPYYTADIILDLRQHRYDLCIVHTPINDPAME
CIQLSNEPMVVAYDKNHPRLGNEITLEEYLEEKHVLHSMWDTKESPLTKTSKYAKSRKVARKAPGPFEMLDMVRGTDWLC
ISQKSILERYGLLDDFKLSPLPFESDLGTEYLVWHKSRNADMAHSWFRKKIVDASQKKPQWGFN
>Mature_304_residues
MKNTDFSLIPYFVVMMEELSISNTAKRFGVTQPAISKALNRLKETYDDPLFVKTKEGYRPSSFCYDIYPQIKQAYDAFES
TFPSKRKFDPTKIERSFNVACISGATYSLMKRVSDILYAETPDIGLHIDPYYTADIILDLRQHRYDLCIVHTPINDPAME
CIQLSNEPMVVAYDKNHPRLGNEITLEEYLEEKHVLHSMWDTKESPLTKTSKYAKSRKVARKAPGPFEMLDMVRGTDWLC
ISQKSILERYGLLDDFKLSPLPFESDLGTEYLVWHKSRNADMAHSWFRKKIVDASQKKPQWGFN

Specific function: Unknown

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87081684, Length=299, Percent_Identity=23.0769230769231, Blast_Score=115, Evalue=4e-27,
Organism=Escherichia coli, GI1786820, Length=291, Percent_Identity=22.680412371134, Blast_Score=69, Evalue=3e-13,

Paralogues:

None

Copy number: 825 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 35285; Mature: 35285

Theoretical pI: Translated: 7.56; Mature: 7.56

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNTDFSLIPYFVVMMEELSISNTAKRFGVTQPAISKALNRLKETYDDPLFVKTKEGYRP
CCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCC
SSFCYDIYPQIKQAYDAFESTFPSKRKFDPTKIERSFNVACISGATYSLMKRVSDILYAE
CCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCCCEEEECCCHHHHHHHHHHHHHCC
TPDIGLHIDPYYTADIILDLRQHRYDLCIVHTPINDPAMECIQLSNEPMVVAYDKNHPRL
CCCCCEEECCCHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHCCCCCEEEEECCCCCCC
GNEITLEEYLEEKHVLHSMWDTKESPLTKTSKYAKSRKVARKAPGPFEMLDMVRGTDWLC
CCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCEEE
ISQKSILERYGLLDDFKLSPLPFESDLGTEYLVWHKSRNADMAHSWFRKKIVDASQKKPQ
ECHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHCCCCCCCC
WGFN
CCCC
>Mature Secondary Structure
MKNTDFSLIPYFVVMMEELSISNTAKRFGVTQPAISKALNRLKETYDDPLFVKTKEGYRP
CCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCC
SSFCYDIYPQIKQAYDAFESTFPSKRKFDPTKIERSFNVACISGATYSLMKRVSDILYAE
CCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCCCEEEECCCHHHHHHHHHHHHHCC
TPDIGLHIDPYYTADIILDLRQHRYDLCIVHTPINDPAMECIQLSNEPMVVAYDKNHPRL
CCCCCEEECCCHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHCCCCCEEEEECCCCCCC
GNEITLEEYLEEKHVLHSMWDTKESPLTKTSKYAKSRKVARKAPGPFEMLDMVRGTDWLC
CCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCEEE
ISQKSILERYGLLDDFKLSPLPFESDLGTEYLVWHKSRNADMAHSWFRKKIVDASQKKPQ
ECHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHCCCCCCCC
WGFN
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11677609; 3519576 [H]