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The map label for this gene is amtB [C]

Identifier: 218709472

GI number: 218709472

Start: 1613027

End: 1614361

Strand: Reverse

Name: amtB [C]

Synonym: VS_1481

Alternate gene names: 218709472

Gene position: 1614361-1613027 (Counterclockwise)

Preceding gene: 218709473

Following gene: 218709471

Centisome position: 48.93

GC content: 47.04

Gene sequence:

>1335_bases
TTGCGTAACCAAGTTGGAACACATCTTGCGTTTACTGTTGAGTCTTTAATTAATTACGACATCAATTCGCACGGTTCTAG
TGCAATTGAGACAAAGCTAAGGGAGAGAGCTTTAATGAGTCAAACGGTTAACCAAGTTCATAGCGCAGTACAGAGCTTGA
CGCAGAGTTCGGATACTCTGTTTTTATTACTAGGCGCGATCATGGTCTTCTTAATGCATGCTGGTTTTGCATTTTTGGAA
GTCGGTACAGTTAGAAAGAAGAACCAAGTTAATGCGTTGGTCAAGATCCTCTCGGACTTTGGAGTCTCCGCCATTGCTTA
CTTTTTTATCGGTTATTGGGTCGCTTACGGCGCGCACTTTTTTGCCGACGCAGAAACGCTATCGCAAGGGAATGGATACG
AACTAGTTAAGTTTTTCTTCCTAATGACCTTTGCCGCAGCAATCCCTGCGATTGTATCCGGTGGTATTGCAGAACGTGCA
CGTTTCTATCCAATACTCATCGCAACACTGTTCACCGTAGGCTTTGTTTACCCATTCTTTGAAGGCATCATCTGGAACGG
TAATTTTGGTTTCCAAGATTGGCTTGAGGCGCAATTCGGTTATGGCTTCCATGACTTCGCGGGCTCTGTTGTCGTTCATG
GTGTTGGCGGCTGGATTGCATTGGTCGCGGTTTACTTCCTTGGCATGCGCAAAGGCCGTGTCCGCGCCGGCAAACACACT
AACTTCGCACCATCAAACATCCCATTCTTAGCACTAGGTTCTTGGATCTTATGTGTGGGTTGGTTTGGCTTTAACGTGAT
GTCTGCTCAAGCAATCAATGGCATTAGTGGACTCGTGGCGATGAACTCACTCATGGCAATGGTCGGCGGTATTCTTGCGG
CTCTAATTGCAGGTAAAAATGACCCAGGCTTCATTCACAATGGACCTTTAGCTGGTCTCGTCGCTATTTGTGCGGGTTCA
GATTTAATGCACCCACTTGGCGCTTTGGTGACTGGCGGCGTGGCAGGCGCGCTGTTTGTTTACCTATTTACTTACATGCA
AAACAAAACACAGATAGATGATGTACTGGGTGTGTGGCCTCTACACGGCGTATGTGGTGCTTGGGGTGGCATAGCAGCAG
GTATCTTCGGTTCGCAAACATTCTGGGGGCTAGGGGGCGTAAGCTTTACTGCTCAGGTGATTGGTACACTAGCGGGCATT
ACGGTAGCTTTGATCGGTGCATTGGTTGTCTACGGTGTGTTGAGCAAAGTAACTGGCCTAAGGTTAAGTGAAGAAGATGA
GTTTAATGGCGCGGATCTTTCAATCCATAAGATCTCGTCTATCAACGAAGACTAA

Upstream 100 bases:

>100_bases
AGTTATCGAATATTTTCTCTCTCTGAGAACCAATAAAGTGCACCCTAGAAATTAACCACTAAAAATCAAGAACTTAAAAC
CAAACTCTGTTGCACGTTTT

Downstream 100 bases:

>100_bases
CGTCGTTCGTTTTGATGGGGTAAAGCTACATCTAGTAGACATCAGTTAACAAATCTAAGCGGCTTCGGTCGCTTTTTTAT
TTCTGTGAAAAGCAAAACAG

Product: putative ammonium transporter

Products: NH3 [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 444; Mature: 444

Protein sequence:

>444_residues
MRNQVGTHLAFTVESLINYDINSHGSSAIETKLRERALMSQTVNQVHSAVQSLTQSSDTLFLLLGAIMVFLMHAGFAFLE
VGTVRKKNQVNALVKILSDFGVSAIAYFFIGYWVAYGAHFFADAETLSQGNGYELVKFFFLMTFAAAIPAIVSGGIAERA
RFYPILIATLFTVGFVYPFFEGIIWNGNFGFQDWLEAQFGYGFHDFAGSVVVHGVGGWIALVAVYFLGMRKGRVRAGKHT
NFAPSNIPFLALGSWILCVGWFGFNVMSAQAINGISGLVAMNSLMAMVGGILAALIAGKNDPGFIHNGPLAGLVAICAGS
DLMHPLGALVTGGVAGALFVYLFTYMQNKTQIDDVLGVWPLHGVCGAWGGIAAGIFGSQTFWGLGGVSFTAQVIGTLAGI
TVALIGALVVYGVLSKVTGLRLSEEDEFNGADLSIHKISSINED

Sequences:

>Translated_444_residues
MRNQVGTHLAFTVESLINYDINSHGSSAIETKLRERALMSQTVNQVHSAVQSLTQSSDTLFLLLGAIMVFLMHAGFAFLE
VGTVRKKNQVNALVKILSDFGVSAIAYFFIGYWVAYGAHFFADAETLSQGNGYELVKFFFLMTFAAAIPAIVSGGIAERA
RFYPILIATLFTVGFVYPFFEGIIWNGNFGFQDWLEAQFGYGFHDFAGSVVVHGVGGWIALVAVYFLGMRKGRVRAGKHT
NFAPSNIPFLALGSWILCVGWFGFNVMSAQAINGISGLVAMNSLMAMVGGILAALIAGKNDPGFIHNGPLAGLVAICAGS
DLMHPLGALVTGGVAGALFVYLFTYMQNKTQIDDVLGVWPLHGVCGAWGGIAAGIFGSQTFWGLGGVSFTAQVIGTLAGI
TVALIGALVVYGVLSKVTGLRLSEEDEFNGADLSIHKISSINED
>Mature_444_residues
MRNQVGTHLAFTVESLINYDINSHGSSAIETKLRERALMSQTVNQVHSAVQSLTQSSDTLFLLLGAIMVFLMHAGFAFLE
VGTVRKKNQVNALVKILSDFGVSAIAYFFIGYWVAYGAHFFADAETLSQGNGYELVKFFFLMTFAAAIPAIVSGGIAERA
RFYPILIATLFTVGFVYPFFEGIIWNGNFGFQDWLEAQFGYGFHDFAGSVVVHGVGGWIALVAVYFLGMRKGRVRAGKHT
NFAPSNIPFLALGSWILCVGWFGFNVMSAQAINGISGLVAMNSLMAMVGGILAALIAGKNDPGFIHNGPLAGLVAICAGS
DLMHPLGALVTGGVAGALFVYLFTYMQNKTQIDDVLGVWPLHGVCGAWGGIAAGIFGSQTFWGLGGVSFTAQVIGTLAGI
TVALIGALVVYGVLSKVTGLRLSEEDEFNGADLSIHKISSINED

Specific function: Involved In The Uptake Of Ammonia (Probable). [C]

COG id: COG0004

COG function: function code P; Ammonia permease

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ammonium transporter (TC 2.A.49) family [H]

Homologues:

Organism=Escherichia coli, GI1786656, Length=440, Percent_Identity=29.0909090909091, Blast_Score=144, Evalue=8e-36,
Organism=Caenorhabditis elegans, GI17550180, Length=447, Percent_Identity=29.9776286353468, Blast_Score=151, Evalue=7e-37,
Organism=Caenorhabditis elegans, GI17550182, Length=436, Percent_Identity=29.8165137614679, Blast_Score=144, Evalue=1e-34,
Organism=Caenorhabditis elegans, GI32564042, Length=452, Percent_Identity=27.8761061946903, Blast_Score=137, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI133931100, Length=443, Percent_Identity=28.4424379232506, Blast_Score=111, Evalue=6e-25,
Organism=Saccharomyces cerevisiae, GI6325396, Length=416, Percent_Identity=27.1634615384615, Blast_Score=107, Evalue=5e-24,
Organism=Saccharomyces cerevisiae, GI6321559, Length=415, Percent_Identity=27.4698795180723, Blast_Score=104, Evalue=3e-23,
Organism=Saccharomyces cerevisiae, GI6324187, Length=411, Percent_Identity=27.007299270073, Blast_Score=84, Evalue=6e-17,
Organism=Drosophila melanogaster, GI161078314, Length=415, Percent_Identity=29.3975903614458, Blast_Score=125, Evalue=4e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001905
- InterPro:   IPR018047 [H]

Pfam domain/function: PF00909 Ammonium_transp [H]

EC number: NA

Molecular weight: Translated: 47348; Mature: 47348

Theoretical pI: Translated: 6.65; Mature: 6.65

Prosite motif: PS01219 AMMONIUM_TRANSP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCCCCCCCCCCCEEEEECCCCCCC
>Mature Secondary Structure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CCCCCCCCCCCCEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NH3 [Periplasm] [C]

Specific reaction: NH3 [Periplasm] = NH3 [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]