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The map label for this gene is cobB [H]

Identifier: 218709471

GI number: 218709471

Start: 1612035

End: 1612769

Strand: Reverse

Name: cobB [H]

Synonym: VS_1480

Alternate gene names: 218709471

Gene position: 1612769-1612035 (Counterclockwise)

Preceding gene: 218709472

Following gene: 218709464

Centisome position: 48.88

GC content: 45.99

Gene sequence:

>735_bases
ATGCATTTCCCATATCGAAATATCGTAATTCTTACTGGCGCCGGTATCTCTGCAGAGTCGGGGATACAAACATTCCGAGC
ACAAGACGGATTATGGGAAAACCATAAAATCGAGGATGTTGCGACACCTGAAGGTTTTGCAAAAGATCCTGACCTTGTAC
AAGCGTTTTACAACAAGCGTCGTCATGGATTACTGAGTGAAAGTATCTTTCCAAATTCAGCCCACAAGGCGCTAGGAGAG
CTTGAAGATAAGCTCGACGGCAAAGTAACCATCATCACTCAGAACATCGACAACCTGCACGAGAGAGGCGGTAGCAACAA
CATTATCCACATGCACGGTGAGCTTCTTAAGGCACGTTGTAGCGAGTCAAACCAAGTTATAGAGCACAAAGACGACATTC
ATACTGGTGAATTGTGCCATTGTTGCCAGATACCAGCTCAAATGCGTCCTCATATTGTGTGGTTTGGCGAAATGCCTTTG
AGAATGGGTGACATTTATTCAGCGTTAGAAGAAGCTGATTTGTTCATCTCGATCGGCACGTCAGGTGTGGTTTATCCAGC
CGCAGGTTTTGTACATGATGCTAAAATGCATGGAGCACATACGATCGAGATAAACCTCGAACCCAGTGCGGTAGAGAGTG
AATTTGAAGAGAAACGTTACGGTAAGGCGAGTATCGAGGTACCTAAGCTAGTCGGTGAGCTATTGCTGTCAGAGAAGGGG
TCTCTAACGGCCTGA

Upstream 100 bases:

>100_bases
GAATTCAGTTGGCTAAGACTATGGTCTAATCCCTAAAACTATTGTTGGAATTTGAACAGTGCTAAGCTGTTACCAAGTGA
CATATTGAAAAGGACGTTAT

Downstream 100 bases:

>100_bases
CCCTTGTGCCAATAAATTGATTGACCCGCTTAAGGTTGGAACTTGAGAGCTCGTTATCAGCAACAGTTATCAGTGGCAAA
CTGACATTTATTCAGATGAG

Product: NAD-dependent deacetylase

Products: NA

Alternate protein names: Regulatory protein SIR2 homolog [H]

Number of amino acids: Translated: 244; Mature: 244

Protein sequence:

>244_residues
MHFPYRNIVILTGAGISAESGIQTFRAQDGLWENHKIEDVATPEGFAKDPDLVQAFYNKRRHGLLSESIFPNSAHKALGE
LEDKLDGKVTIITQNIDNLHERGGSNNIIHMHGELLKARCSESNQVIEHKDDIHTGELCHCCQIPAQMRPHIVWFGEMPL
RMGDIYSALEEADLFISIGTSGVVYPAAGFVHDAKMHGAHTIEINLEPSAVESEFEEKRYGKASIEVPKLVGELLLSEKG
SLTA

Sequences:

>Translated_244_residues
MHFPYRNIVILTGAGISAESGIQTFRAQDGLWENHKIEDVATPEGFAKDPDLVQAFYNKRRHGLLSESIFPNSAHKALGE
LEDKLDGKVTIITQNIDNLHERGGSNNIIHMHGELLKARCSESNQVIEHKDDIHTGELCHCCQIPAQMRPHIVWFGEMPL
RMGDIYSALEEADLFISIGTSGVVYPAAGFVHDAKMHGAHTIEINLEPSAVESEFEEKRYGKASIEVPKLVGELLLSEKG
SLTA
>Mature_244_residues
MHFPYRNIVILTGAGISAESGIQTFRAQDGLWENHKIEDVATPEGFAKDPDLVQAFYNKRRHGLLSESIFPNSAHKALGE
LEDKLDGKVTIITQNIDNLHERGGSNNIIHMHGELLKARCSESNQVIEHKDDIHTGELCHCCQIPAQMRPHIVWFGEMPL
RMGDIYSALEEADLFISIGTSGVVYPAAGFVHDAKMHGAHTIEINLEPSAVESEFEEKRYGKASIEVPKLVGELLLSEKG
SLTA

Specific function: Modulates the activities of several enzymes which are inactive in their acetylated form [H]

COG id: COG0846

COG function: function code K; NAD-dependent protein deacetylases, SIR2 family

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 deacetylase sirtuin-type domain [H]

Homologues:

Organism=Homo sapiens, GI300795542, Length=233, Percent_Identity=37.7682403433476, Blast_Score=160, Evalue=1e-39,
Organism=Homo sapiens, GI6912664, Length=255, Percent_Identity=34.1176470588235, Blast_Score=150, Evalue=8e-37,
Organism=Homo sapiens, GI13787215, Length=241, Percent_Identity=35.2697095435685, Blast_Score=149, Evalue=2e-36,
Organism=Homo sapiens, GI7657575, Length=224, Percent_Identity=31.25, Blast_Score=82, Evalue=3e-16,
Organism=Homo sapiens, GI6912660, Length=202, Percent_Identity=29.2079207920792, Blast_Score=74, Evalue=9e-14,
Organism=Homo sapiens, GI6912662, Length=238, Percent_Identity=24.3697478991597, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI63054862, Length=197, Percent_Identity=29.4416243654822, Blast_Score=72, Evalue=6e-13,
Organism=Escherichia coli, GI308199517, Length=229, Percent_Identity=65.0655021834061, Blast_Score=323, Evalue=4e-90,
Organism=Caenorhabditis elegans, GI17541892, Length=201, Percent_Identity=29.3532338308458, Blast_Score=78, Evalue=4e-15,
Organism=Drosophila melanogaster, GI28571445, Length=183, Percent_Identity=27.3224043715847, Blast_Score=64, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003000 [H]

Pfam domain/function: PF02146 SIR2 [H]

EC number: 3.5.1.- [C]

Molecular weight: Translated: 27006; Mature: 27006

Theoretical pI: Translated: 5.49; Mature: 5.49

Prosite motif: PS50305 SIRTUIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHFPYRNIVILTGAGISAESGIQTFRAQDGLWENHKIEDVATPEGFAKDPDLVQAFYNKR
CCCCCCCEEEEECCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHH
RHGLLSESIFPNSAHKALGELEDKLDGKVTIITQNIDNLHERGGSNNIIHMHGELLKARC
HCCCHHHCCCCCHHHHHHHHHHHHCCCEEEEEECCHHHHHHCCCCCCEEEECHHHHHHHH
SESNQVIEHKDDIHTGELCHCCQIPAQMRPHIVWFGEMPLRMGDIYSALEEADLFISIGT
CCCCCCHHCCCCCCCCCHHHHHCCCHHCCCCEEEECCCCCHHHHHHHHHHHCCEEEEECC
SGVVYPAAGFVHDAKMHGAHTIEINLEPSAVESEFEEKRYGKASIEVPKLVGELLLSEKG
CCEEECCCCCEEHHHCCCEEEEEEEECCHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCC
SLTA
CCCC
>Mature Secondary Structure
MHFPYRNIVILTGAGISAESGIQTFRAQDGLWENHKIEDVATPEGFAKDPDLVQAFYNKR
CCCCCCCEEEEECCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHH
RHGLLSESIFPNSAHKALGELEDKLDGKVTIITQNIDNLHERGGSNNIIHMHGELLKARC
HCCCHHHCCCCCHHHHHHHHHHHHCCCEEEEEECCHHHHHHCCCCCCEEEECHHHHHHHH
SESNQVIEHKDDIHTGELCHCCQIPAQMRPHIVWFGEMPLRMGDIYSALEEADLFISIGT
CCCCCCHHCCCCCCCCCHHHHHCCCHHCCCCEEEECCCCCHHHHHHHHHHHCCEEEEECC
SGVVYPAAGFVHDAKMHGAHTIEINLEPSAVESEFEEKRYGKASIEVPKLVGELLLSEKG
CCEEECCCCCEEHHHCCCEEEEEEEECCHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCC
SLTA
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12620739 [H]