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The map label for this gene is rlmL [H]

Identifier: 218709415

GI number: 218709415

Start: 1564826

End: 1566946

Strand: Direct

Name: rlmL [H]

Synonym: VS_1424

Alternate gene names: 218709415

Gene position: 1564826-1566946 (Clockwise)

Preceding gene: 218709414

Following gene: 218709416

Centisome position: 47.43

GC content: 43.99

Gene sequence:

>2121_bases
ATGAATCAATATCTAGCGGTTACCTCAAACGGCCTTGAGAATTTATTAGTTGAAGAACTAACCCAACTAGGGATTACAAA
TGCAAAACCTGTTCAAGCAGGTGTTAAATTCAAAGCGACCAATGAGCAAATTTATCGTTGTTGTTTATGGAGTCGTTTGG
CTTCTAGATTTGTACGTGTCCTTTCTGAATTCACTTGTATGGACGACATGGATTTGTACCTATCAACCACCGCGGTTAAC
TGGGTAAATCAATTCCATAGCTCTAAGCGTTTTGTTGTTGACTTTAACGGTACAAACAACGAAATCCGTAATAGCCAATA
CGGTGCGATGAAAGTAAAAGATGCCGTTGTTGATAGCTTTGAGAAAAAGTCTTTGCCTCGTCCGTCTATCAGCAAAGAAA
ATCCAGATATTCGTATTCACGTTCGTCTACATCGTGATAAAGCGATTCTTGGTGTTGATATGGTAGGTAGCGGTCTTCAC
CAACGTGGTTACCGTCCAGAATCAGGCCGAGCACCATTGCGTGAAACCCTTGCTGCTGCGATTCTTCTTCGTAGTGGTTG
GGATGCGACTAAGCCTTTCCTAGACCCAATGTGTGGTTCAGGTACGTTAGTGATTGAAGCGGCAATGATGGCTGCGAACA
TGGCTCCAGGTGTTAAACGCCAGAGGTGGTGTTTTGAATCACTAGAAGATTTTGAACCAGAACTGTGGGCTGAAGTGAAA
GCTGAAGCGAACGTTCAAGGTCGTCGTGGCGTTAAGAAAGTAGAGTGTAAGTTCTATGGCTACGACAATGACGAGCGCAT
GATCAAAACAGCGCGTGACAATGCTCGTCGTGCTGGTGTTGAAGAGCTGATTGAATTCGAAGTAGGCGATGCTGCAAAGC
TTAAGCGTCCTACAGAGTTTACTGATGGTGTGATTGTTTCAAACCCTCCTTATGGTGAGCGTCTTGGCACAGAGCCGGGT
CTTATTGCACTTTACACGGCATTCGGTGCACAACTTAAAGCGGAATTTGGCGGTTGTAACGCGTCTATCTTCTCTAGTTC
TGACGAACTGCTTAGCTGCTTACGTATGCGTGCAGACAAACAGTTCAAATTGAACAATGGTGCGTTACCGTGTCACCAAA
AGAACTACTCAATTTCAGATCGTCCGATGTCAGAGCGTCCAACGGGTGAACAAGAGCAACTTATTGCTCCAGACTTCGCA
AACCGTCTTAAAAAGAACATCGGTAAAATTGGCAAGTGGGCTAAGAAAGAGCAATTAGATTGTTACCGTATCTACGATGC
GGACTTACCAGAATACAATGTAGCGATTGACGTATACCCAGGTCACCTAGTGATTCAAGAATACGCAGCACCTAAAGATG
TACCGGAAGAGAAAGCAAAACGTCGCTTAACCGATATCATCCGTGCTTCTATTCAAGTCACTGGCGTTGAAGCAAACAAC
GTGGTTCTTAAGGTTCGTCAGAAGCAGAAAGGCCGATCTCAATATCAAAAAATGGCTCAAGACTCCTCTAACCTAGAAGT
GAACGAATACGGCGTTAAGCTGATTGTTAATCTTCATGACTACTTAGATACGGGCTTGTTCTTAGATCATAAGATCACTC
GTCGTCGTATTGGCGAAATGGCTGCAGGTAAAGATTTCCTTAACTTATTTGCTTACACAGGCAGTGCATCTGTTCATGCT
GCTGTGGGTGGCGCACGCTCTACAACAACCGTTGATATGTCTAATACCTACCTTGAGTGGGCAAAACAGAACATGGAGCT
TAACGGCCGTGTTGGTCGTCAACATCAGTTTGTTCAAGCTGACTGCTTACAGTGGTTGGTTAAAGAGCAGGGTTCTTACG
ACCTGATCTTCATTGATCCCCCAACGTTCTCAAACTCTAAGCGTATGGATCAATCTTTTGATGTTCAGCGTGATCACATT
CAATTGATGGAAAACCTTAAGCGTCTTCTTCGTGAAGAAGGCACAATTGTGTTCTCTAACAACAAGCGTCACTTCAAAAT
GGATGTAGAAGGCCTAGAAGAGTTAGGTCTCAAAGCTCAGAACATCTCAGCTAAAACGCTTCCATTGGACTTCTCTCGTA
ACAAGCACATTCATAACTGCTGGTTGATTACGCATAAGTAA

Upstream 100 bases:

>100_bases
CCAGTTAGTCGAAAGATTCAATGAAAAGACAAGTTTAGTGTGATGCTCTAATAACAAATTCAGGTATAATCGAGCTAATT
TTTATCAATAAAAGAACACT

Downstream 100 bases:

>100_bases
TTAAGAGATTGTATAAGGATATAGCGTGCTGACTCTCTACAGTACAGAAGGGTGCCATCTATGTGAGATGGCATTCAAAC
TCACGGAACAGTTAAACATT

Product: 23S rRNA m(2)G2445 methyltransferase

Products: NA

Alternate protein names: 23S rRNA m2G2445 methyltransferase; rRNA (guanine-N(2)-)-methyltransferase rlmL [H]

Number of amino acids: Translated: 706; Mature: 706

Protein sequence:

>706_residues
MNQYLAVTSNGLENLLVEELTQLGITNAKPVQAGVKFKATNEQIYRCCLWSRLASRFVRVLSEFTCMDDMDLYLSTTAVN
WVNQFHSSKRFVVDFNGTNNEIRNSQYGAMKVKDAVVDSFEKKSLPRPSISKENPDIRIHVRLHRDKAILGVDMVGSGLH
QRGYRPESGRAPLRETLAAAILLRSGWDATKPFLDPMCGSGTLVIEAAMMAANMAPGVKRQRWCFESLEDFEPELWAEVK
AEANVQGRRGVKKVECKFYGYDNDERMIKTARDNARRAGVEELIEFEVGDAAKLKRPTEFTDGVIVSNPPYGERLGTEPG
LIALYTAFGAQLKAEFGGCNASIFSSSDELLSCLRMRADKQFKLNNGALPCHQKNYSISDRPMSERPTGEQEQLIAPDFA
NRLKKNIGKIGKWAKKEQLDCYRIYDADLPEYNVAIDVYPGHLVIQEYAAPKDVPEEKAKRRLTDIIRASIQVTGVEANN
VVLKVRQKQKGRSQYQKMAQDSSNLEVNEYGVKLIVNLHDYLDTGLFLDHKITRRRIGEMAAGKDFLNLFAYTGSASVHA
AVGGARSTTTVDMSNTYLEWAKQNMELNGRVGRQHQFVQADCLQWLVKEQGSYDLIFIDPPTFSNSKRMDQSFDVQRDHI
QLMENLKRLLREEGTIVFSNNKRHFKMDVEGLEELGLKAQNISAKTLPLDFSRNKHIHNCWLITHK

Sequences:

>Translated_706_residues
MNQYLAVTSNGLENLLVEELTQLGITNAKPVQAGVKFKATNEQIYRCCLWSRLASRFVRVLSEFTCMDDMDLYLSTTAVN
WVNQFHSSKRFVVDFNGTNNEIRNSQYGAMKVKDAVVDSFEKKSLPRPSISKENPDIRIHVRLHRDKAILGVDMVGSGLH
QRGYRPESGRAPLRETLAAAILLRSGWDATKPFLDPMCGSGTLVIEAAMMAANMAPGVKRQRWCFESLEDFEPELWAEVK
AEANVQGRRGVKKVECKFYGYDNDERMIKTARDNARRAGVEELIEFEVGDAAKLKRPTEFTDGVIVSNPPYGERLGTEPG
LIALYTAFGAQLKAEFGGCNASIFSSSDELLSCLRMRADKQFKLNNGALPCHQKNYSISDRPMSERPTGEQEQLIAPDFA
NRLKKNIGKIGKWAKKEQLDCYRIYDADLPEYNVAIDVYPGHLVIQEYAAPKDVPEEKAKRRLTDIIRASIQVTGVEANN
VVLKVRQKQKGRSQYQKMAQDSSNLEVNEYGVKLIVNLHDYLDTGLFLDHKITRRRIGEMAAGKDFLNLFAYTGSASVHA
AVGGARSTTTVDMSNTYLEWAKQNMELNGRVGRQHQFVQADCLQWLVKEQGSYDLIFIDPPTFSNSKRMDQSFDVQRDHI
QLMENLKRLLREEGTIVFSNNKRHFKMDVEGLEELGLKAQNISAKTLPLDFSRNKHIHNCWLITHK
>Mature_706_residues
MNQYLAVTSNGLENLLVEELTQLGITNAKPVQAGVKFKATNEQIYRCCLWSRLASRFVRVLSEFTCMDDMDLYLSTTAVN
WVNQFHSSKRFVVDFNGTNNEIRNSQYGAMKVKDAVVDSFEKKSLPRPSISKENPDIRIHVRLHRDKAILGVDMVGSGLH
QRGYRPESGRAPLRETLAAAILLRSGWDATKPFLDPMCGSGTLVIEAAMMAANMAPGVKRQRWCFESLEDFEPELWAEVK
AEANVQGRRGVKKVECKFYGYDNDERMIKTARDNARRAGVEELIEFEVGDAAKLKRPTEFTDGVIVSNPPYGERLGTEPG
LIALYTAFGAQLKAEFGGCNASIFSSSDELLSCLRMRADKQFKLNNGALPCHQKNYSISDRPMSERPTGEQEQLIAPDFA
NRLKKNIGKIGKWAKKEQLDCYRIYDADLPEYNVAIDVYPGHLVIQEYAAPKDVPEEKAKRRLTDIIRASIQVTGVEANN
VVLKVRQKQKGRSQYQKMAQDSSNLEVNEYGVKLIVNLHDYLDTGLFLDHKITRRRIGEMAAGKDFLNLFAYTGSASVHA
AVGGARSTTTVDMSNTYLEWAKQNMELNGRVGRQHQFVQADCLQWLVKEQGSYDLIFIDPPTFSNSKRMDQSFDVQRDHI
QLMENLKRLLREEGTIVFSNNKRHFKMDVEGLEELGLKAQNISAKTLPLDFSRNKHIHNCWLITHK

Specific function: Specifically methylates the guanosine in position 2445 (m2G2445) of 23S rRNA [H]

COG id: COG0116

COG function: function code L; Predicted N6-adenine-specific DNA methylase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 THUMP domain [H]

Homologues:

Organism=Escherichia coli, GI1787181, Length=705, Percent_Identity=56.3120567375887, Blast_Score=832, Evalue=0.0,
Organism=Escherichia coli, GI145693111, Length=239, Percent_Identity=27.1966527196653, Blast_Score=79, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR002296
- InterPro:   IPR000241
- InterPro:   IPR017244
- InterPro:   IPR019614
- InterPro:   IPR004114 [H]

Pfam domain/function: PF10672 Methyltrans_SAM; PF02926 THUMP; PF01170 UPF0020 [H]

EC number: =2.1.1.173 [H]

Molecular weight: Translated: 79902; Mature: 79902

Theoretical pI: Translated: 8.49; Mature: 8.49

Prosite motif: PS51165 THUMP ; PS00092 N6_MTASE ; PS01261 UPF0020

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNQYLAVTSNGLENLLVEELTQLGITNAKPVQAGVKFKATNEQIYRCCLWSRLASRFVRV
CCCEEEEECCCHHHHHHHHHHHHCCCCCCCHHCCCEEEECHHHHHHHHHHHHHHHHHHHH
LSEFTCMDDMDLYLSTTAVNWVNQFHSSKRFVVDFNGTNNEIRNSQYGAMKVKDAVVDSF
HHHHCCCCCCHHEEEHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCEEHHHHHHHHH
EKKSLPRPSISKENPDIRIHVRLHRDKAILGVDMVGSGLHQRGYRPESGRAPLRETLAAA
HHHCCCCCCCCCCCCCEEEEEEEECCCEEEEHHHHCCCHHHCCCCCCCCCCHHHHHHHHH
ILLRSGWDATKPFLDPMCGSGTLVIEAAMMAANMAPGVKRQRWCFESLEDFEPELWAEVK
HHHHCCCCCCCHHHCCCCCCCCEEHHHHHHHHHCCCCCHHHHHHHHHHHHCCHHHHHHHH
AEANVQGRRGVKKVECKFYGYDNDERMIKTARDNARRAGVEELIEFEVGDAAKLKRPTEF
HCCCCCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCC
TDGVIVSNPPYGERLGTEPGLIALYTAFGAQLKAEFGGCNASIFSSSDELLSCLRMRADK
CCCEEECCCCCCHHCCCCCCEEEEEEHHCCCEEHHCCCCCCEEECCHHHHHHHHHHCCCC
QFKLNNGALPCHQKNYSISDRPMSERPTGEQEQLIAPDFANRLKKNIGKIGKWAKKEQLD
CEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCEECCHHHHHHHHHHHHHHCHHHHCCCC
CYRIYDADLPEYNVAIDVYPGHLVIQEYAAPKDVPEEKAKRRLTDIIRASIQVTGVEANN
EEEEECCCCCCCEEEEEECCCEEEEEECCCCCCCCHHHHHHHHHHHHHCCEEEEEEECCC
VVLKVRQKQKGRSQYQKMAQDSSNLEVNEYGVKLIVNLHDYLDTGLFLDHKITRRRIGEM
EEEEEHHHHHHHHHHHHHHCCCCCCEEECCCEEEEEEEHHHHCCCCEECHHHHHHHHHHH
AAGKDFLNLFAYTGSASVHAAVGGARSTTTVDMSNTYLEWAKQNMELNGRVGRQHQFVQA
HCCHHHHHHHHCCCCCCEEEECCCCCCCEEEECCHHHHHHHHCCCCCCCCCCCCHHHHHH
DCLQWLVKEQGSYDLIFIDPPTFSNSKRMDQSFDVQRDHIQLMENLKRLLREEGTIVFSN
HHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEC
NKRHFKMDVEGLEELGLKAQNISAKTLPLDFSRNKHIHNCWLITHK
CCEEEEECHHHHHHHCCEECCCCCEEEEEECCCCCCEEEEEEEEEC
>Mature Secondary Structure
MNQYLAVTSNGLENLLVEELTQLGITNAKPVQAGVKFKATNEQIYRCCLWSRLASRFVRV
CCCEEEEECCCHHHHHHHHHHHHCCCCCCCHHCCCEEEECHHHHHHHHHHHHHHHHHHHH
LSEFTCMDDMDLYLSTTAVNWVNQFHSSKRFVVDFNGTNNEIRNSQYGAMKVKDAVVDSF
HHHHCCCCCCHHEEEHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCEEHHHHHHHHH
EKKSLPRPSISKENPDIRIHVRLHRDKAILGVDMVGSGLHQRGYRPESGRAPLRETLAAA
HHHCCCCCCCCCCCCCEEEEEEEECCCEEEEHHHHCCCHHHCCCCCCCCCCHHHHHHHHH
ILLRSGWDATKPFLDPMCGSGTLVIEAAMMAANMAPGVKRQRWCFESLEDFEPELWAEVK
HHHHCCCCCCCHHHCCCCCCCCEEHHHHHHHHHCCCCCHHHHHHHHHHHHCCHHHHHHHH
AEANVQGRRGVKKVECKFYGYDNDERMIKTARDNARRAGVEELIEFEVGDAAKLKRPTEF
HCCCCCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCC
TDGVIVSNPPYGERLGTEPGLIALYTAFGAQLKAEFGGCNASIFSSSDELLSCLRMRADK
CCCEEECCCCCCHHCCCCCCEEEEEEHHCCCEEHHCCCCCCEEECCHHHHHHHHHHCCCC
QFKLNNGALPCHQKNYSISDRPMSERPTGEQEQLIAPDFANRLKKNIGKIGKWAKKEQLD
CEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCEECCHHHHHHHHHHHHHHCHHHHCCCC
CYRIYDADLPEYNVAIDVYPGHLVIQEYAAPKDVPEEKAKRRLTDIIRASIQVTGVEANN
EEEEECCCCCCCEEEEEECCCEEEEEECCCCCCCCHHHHHHHHHHHHHCCEEEEEEECCC
VVLKVRQKQKGRSQYQKMAQDSSNLEVNEYGVKLIVNLHDYLDTGLFLDHKITRRRIGEM
EEEEEHHHHHHHHHHHHHHCCCCCCEEECCCEEEEEEEHHHHCCCCEECHHHHHHHHHHH
AAGKDFLNLFAYTGSASVHAAVGGARSTTTVDMSNTYLEWAKQNMELNGRVGRQHQFVQA
HCCHHHHHHHHCCCCCCEEEECCCCCCCEEEECCHHHHHHHHCCCCCCCCCCCCHHHHHH
DCLQWLVKEQGSYDLIFIDPPTFSNSKRMDQSFDVQRDHIQLMENLKRLLREEGTIVFSN
HHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEC
NKRHFKMDVEGLEELGLKAQNISAKTLPLDFSRNKHIHNCWLITHK
CCEEEEECHHHHHHHCCEECCCCCEEEEEECCCCCCEEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA