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The map label for this gene is rpfG [H]

Identifier: 218709307

GI number: 218709307

Start: 1417460

End: 1418503

Strand: Reverse

Name: rpfG [H]

Synonym: VS_1316

Alternate gene names: 218709307

Gene position: 1418503-1417460 (Counterclockwise)

Preceding gene: 218709308

Following gene: 218709306

Centisome position: 42.99

GC content: 43.58

Gene sequence:

>1044_bases
ATGAATCAAATTTCGACAGATAAACCTATTATTTTGGTTGTTGATGACATCCCGGATAATATCCACACCCTGTCAGGTAT
CCTCAATGAAGACTTTAAAATTAAAGCTGCGACATCAGGTCAAAAAGCGTTAAAGATTGCCTCTTCACATCCAAAACCAC
ACCTGATCCTGCTCGATATAATGATGCCAGAAATGGATGGTTATGAAGTGTGTCGCCGACTTAAAAATGATCCAGTCACC
GCCAATATCCCTGTAATTTTTGTTACCGCAAAAACTGATATGGTCGATGAACAAATGGGCTTTGAGCTAGGTGCTGTTGA
TTACATCACTAAGCCAGTTAGCCCACCTATTGTGAAAGCTCGTGTTGTCACGCAAATCTCCCTCTACGACCAAAACTTGG
CACTTTCTCGCAAAGTAAGGCAGAGAACGGAAGCTCTGGCAATGAGTCGATTGGAGATTATTCGCAAACTCGGTCGTGCA
GCAGAGTACAAAGACAATGAAACGGGAATGCACGTTGTAAGAATGAGTTACTACTCGAAAATTCTGGCTCAACACTTGGA
CGTTAATGAGGATTGGGTAGAACTGCTTTTCCAAGCCGCACCAATGCATGACATAGGGAAAATTGGTATCCCTGATTCCA
TATTAGGCAAACCAGGAAAGCTGGATGCTGAAGAGTGGGCAATCATGCAAACACACGTCACCATTGGTGGGGACATCATT
GGTGATAACGATTCCAAACTACTAAAACTCGCCCAAGAAATTGCGCTATATCATCATGAAAAATGGGATGGTAGCGGCTA
CCCTCACAAGCTATCCGGTGAAGATATCCCTCTCAGCGCGCGAATTGTAGCGATTGCCGACGTATTCGACGCACTGACTA
GCGAACGCCCATACAAAGAAGCTTGGCCTATTCAAAAAGCGATTGAGCTGATTCAAGAACAAGCTGGCCATCATTTTGAC
CCAAACTTAGTGCCTATCTTCATAAATAAGCTCGACGAGATGTTAGCGATCAAAGACTCTTTTTCTGATGAACCAGAACA
CTAA

Upstream 100 bases:

>100_bases
ATACCAAAAGTTCCCCCCTCAGAACCATGCATTTCCAATAAAACCCACTACGCTTTAACTATGTGGTATCGCCGTTGGCT
TAACTTTTTTAGGGACAAAA

Downstream 100 bases:

>100_bases
GTTCGCTGGTACTTTATCAATACAGGTACTTTATGAATACTGTTTCAAGATTTGCTACTGATTCTAAAATAATCTTAATC
TCTTTTCTCGTTGCGGCTGT

Product: putative two-component regulatory system response regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 347; Mature: 347

Protein sequence:

>347_residues
MNQISTDKPIILVVDDIPDNIHTLSGILNEDFKIKAATSGQKALKIASSHPKPHLILLDIMMPEMDGYEVCRRLKNDPVT
ANIPVIFVTAKTDMVDEQMGFELGAVDYITKPVSPPIVKARVVTQISLYDQNLALSRKVRQRTEALAMSRLEIIRKLGRA
AEYKDNETGMHVVRMSYYSKILAQHLDVNEDWVELLFQAAPMHDIGKIGIPDSILGKPGKLDAEEWAIMQTHVTIGGDII
GDNDSKLLKLAQEIALYHHEKWDGSGYPHKLSGEDIPLSARIVAIADVFDALTSERPYKEAWPIQKAIELIQEQAGHHFD
PNLVPIFINKLDEMLAIKDSFSDEPEH

Sequences:

>Translated_347_residues
MNQISTDKPIILVVDDIPDNIHTLSGILNEDFKIKAATSGQKALKIASSHPKPHLILLDIMMPEMDGYEVCRRLKNDPVT
ANIPVIFVTAKTDMVDEQMGFELGAVDYITKPVSPPIVKARVVTQISLYDQNLALSRKVRQRTEALAMSRLEIIRKLGRA
AEYKDNETGMHVVRMSYYSKILAQHLDVNEDWVELLFQAAPMHDIGKIGIPDSILGKPGKLDAEEWAIMQTHVTIGGDII
GDNDSKLLKLAQEIALYHHEKWDGSGYPHKLSGEDIPLSARIVAIADVFDALTSERPYKEAWPIQKAIELIQEQAGHHFD
PNLVPIFINKLDEMLAIKDSFSDEPEH
>Mature_347_residues
MNQISTDKPIILVVDDIPDNIHTLSGILNEDFKIKAATSGQKALKIASSHPKPHLILLDIMMPEMDGYEVCRRLKNDPVT
ANIPVIFVTAKTDMVDEQMGFELGAVDYITKPVSPPIVKARVVTQISLYDQNLALSRKVRQRTEALAMSRLEIIRKLGRA
AEYKDNETGMHVVRMSYYSKILAQHLDVNEDWVELLFQAAPMHDIGKIGIPDSILGKPGKLDAEEWAIMQTHVTIGGDII
GDNDSKLLKLAQEIALYHHEKWDGSGYPHKLSGEDIPLSARIVAIADVFDALTSERPYKEAWPIQKAIELIQEQAGHHFD
PNLVPIFINKLDEMLAIKDSFSDEPEH

Specific function: Positively regulates the synthesis of extracellular enzymes and extracellular polysaccharides. The cell-cell signaling system encoded by rpf genes is required for full virulence and is implicated in regulation of the formation and dispersal of biofilms [H

COG id: COG3437

COG function: function code KT; Response regulator containing a CheY-like receiver domain and an HD-GYP domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1788394, Length=122, Percent_Identity=38.5245901639344, Blast_Score=76, Evalue=4e-15,
Organism=Escherichia coli, GI1789809, Length=113, Percent_Identity=37.1681415929204, Blast_Score=72, Evalue=7e-14,
Organism=Escherichia coli, GI1790860, Length=136, Percent_Identity=32.3529411764706, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1786599, Length=74, Percent_Identity=44.5945945945946, Blast_Score=65, Evalue=8e-12,
Organism=Escherichia coli, GI1790863, Length=146, Percent_Identity=30.8219178082192, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR003607
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 38881; Mature: 38881

Theoretical pI: Translated: 5.26; Mature: 5.26

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNQISTDKPIILVVDDIPDNIHTLSGILNEDFKIKAATSGQKALKIASSHPKPHLILLDI
CCCCCCCCCEEEEEECCCCHHHHHHHHHCCCCEEEEECCCCHHHHHHCCCCCCEEEEEEE
MMPEMDGYEVCRRLKNDPVTANIPVIFVTAKTDMVDEQMGFELGAVDYITKPVSPPIVKA
ECCCCCHHHHHHHHCCCCCEECCCEEEEEECCCHHHHHHCCCCCCHHHHHCCCCCCHHHH
RVVTQISLYDQNLALSRKVRQRTEALAMSRLEIIRKLGRAAEYKDNETGMHVVRMSYYSK
HHHEEEEHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHH
ILAQHLDVNEDWVELLFQAAPMHDIGKIGIPDSILGKPGKLDAEEWAIMQTHVTIGGDII
HHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCHHHCCCCCCCCHHHHEEEEEEEEECCEEE
GDNDSKLLKLAQEIALYHHEKWDGSGYPHKLSGEDIPLSARIVAIADVFDALTSERPYKE
CCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCHH
AWPIQKAIELIQEQAGHHFDPNLVPIFINKLDEMLAIKDSFSDEPEH
HCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MNQISTDKPIILVVDDIPDNIHTLSGILNEDFKIKAATSGQKALKIASSHPKPHLILLDI
CCCCCCCCCEEEEEECCCCHHHHHHHHHCCCCEEEEECCCCHHHHHHCCCCCCEEEEEEE
MMPEMDGYEVCRRLKNDPVTANIPVIFVTAKTDMVDEQMGFELGAVDYITKPVSPPIVKA
ECCCCCHHHHHHHHCCCCCEECCCEEEEEECCCHHHHHHCCCCCCHHHHHCCCCCCHHHH
RVVTQISLYDQNLALSRKVRQRTEALAMSRLEIIRKLGRAAEYKDNETGMHVVRMSYYSK
HHHEEEEHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHH
ILAQHLDVNEDWVELLFQAAPMHDIGKIGIPDSILGKPGKLDAEEWAIMQTHVTIGGDII
HHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCHHHCCCCCCCCHHHHEEEEEEEEECCEEE
GDNDSKLLKLAQEIALYHHEKWDGSGYPHKLSGEDIPLSARIVAIADVFDALTSERPYKE
CCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCHH
AWPIQKAIELIQEQAGHHFDPNLVPIFINKLDEMLAIKDSFSDEPEH
HCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11123673 [H]