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The map label for this gene is argT [H]

Identifier: 218709049

GI number: 218709049

Start: 1106169

End: 1106939

Strand: Direct

Name: argT [H]

Synonym: VS_1055

Alternate gene names: 218709049

Gene position: 1106169-1106939 (Clockwise)

Preceding gene: 218709048

Following gene: 218709050

Centisome position: 33.53

GC content: 43.97

Gene sequence:

>771_bases
ATGAAAAAGTGGTTATTAGTCGCGGCACTTGCTGCAACTGCTGCAACGGGCGTAGCTCAAGCAAAAGAATGGAAAACAGT
ACGTTTCGGTATTGAAGGTGCTTACCCTCCATTTAGCTGGACAGAAGCTGACGGTTCGCTGAAAGGCTTCGATGTCGATA
TGGCTAACGCGCTTTGTACTGAAATGCAGGTGAAGTGTAAGATCGTTGCACAAGATTGGGATGGTATTATTCCTTCTCTG
CTTGCTCGTAAATATGATGCGATCATTGCGGCAATGTCTATCACGGAAGAGCGCAAGAAAAAGATCGATTTCACAGGTAA
ATACGCACTTATCCCAAACAAGTTCATCGCTAAAAAAGGTGCAGGCCTTAACTTTGATGATCTAAGTGGTCAAAAAATTG
CCGTTCAACGTGCAACAACTCACGACAAGTACCTAACAGATAACTACGGCGACACAGTAGAAATCGTTCGTTACGGTTCA
TTTGACGAAGCTTACCTTGATCTAGCTAACGGTCGTGTTGCTGCTGTACTGGGTGATGCATCTGCTCTAGAAGAAGGTGT
ACTAAACAAAGCGGGTGGTGAAGACTACGAGTTTGTTGGTCCATCACTCACTGATCCTAAGTGGTTCGGTGAAGGTTTTG
GTATTGCTCTACGTAAGCAAGACAAAGATCTGACTAAGCAATTAGATGCTGCAATCCTTTCGCTACGTGAAAAAGGCATC
TACCAAGATATCGCTGCTAAATACTTCAACTACGACGTATACGGTAAGTAA

Upstream 100 bases:

>100_bases
CAGTGTTAACAATGGAACACAACAAGTAAGTTAATAAGTAACAACAAACACAATATTAAAATTAGCAAAACATAAAAACC
TAAATCACAGGAGTAGGGAT

Downstream 100 bases:

>100_bases
TCTCTAGCGTCACTTCTTAAGGGAGAGGCTCACCTCTCCCTGTCTTACCAACTTTTTCGCAGTTCTGTTGGAATTCATTA
TGTTTGATTTACAAGGATAT

Product: lysine-arginine-ornithine-binding periplasmic protein

Products: ADP; phosphate; L-ornithine [Cytoplasm]; ADP; L-arginine [Cytoplasm]; L-lysine [Cytoplasm] [C]

Alternate protein names: LAO-binding protein [H]

Number of amino acids: Translated: 256; Mature: 256

Protein sequence:

>256_residues
MKKWLLVAALAATAATGVAQAKEWKTVRFGIEGAYPPFSWTEADGSLKGFDVDMANALCTEMQVKCKIVAQDWDGIIPSL
LARKYDAIIAAMSITEERKKKIDFTGKYALIPNKFIAKKGAGLNFDDLSGQKIAVQRATTHDKYLTDNYGDTVEIVRYGS
FDEAYLDLANGRVAAVLGDASALEEGVLNKAGGEDYEFVGPSLTDPKWFGEGFGIALRKQDKDLTKQLDAAILSLREKGI
YQDIAAKYFNYDVYGK

Sequences:

>Translated_256_residues
MKKWLLVAALAATAATGVAQAKEWKTVRFGIEGAYPPFSWTEADGSLKGFDVDMANALCTEMQVKCKIVAQDWDGIIPSL
LARKYDAIIAAMSITEERKKKIDFTGKYALIPNKFIAKKGAGLNFDDLSGQKIAVQRATTHDKYLTDNYGDTVEIVRYGS
FDEAYLDLANGRVAAVLGDASALEEGVLNKAGGEDYEFVGPSLTDPKWFGEGFGIALRKQDKDLTKQLDAAILSLREKGI
YQDIAAKYFNYDVYGK
>Mature_256_residues
MKKWLLVAALAATAATGVAQAKEWKTVRFGIEGAYPPFSWTEADGSLKGFDVDMANALCTEMQVKCKIVAQDWDGIIPSL
LARKYDAIIAAMSITEERKKKIDFTGKYALIPNKFIAKKGAGLNFDDLSGQKIAVQRATTHDKYLTDNYGDTVEIVRYGS
FDEAYLDLANGRVAAVLGDASALEEGVLNKAGGEDYEFVGPSLTDPKWFGEGFGIALRKQDKDLTKQLDAAILSLREKGI
YQDIAAKYFNYDVYGK

Specific function: This periplasmic binding protein is involved in an arginine transport system. ArgT and histidine-binding protein J (hisJ) interact with a common membrane-bound receptor, hisP [H]

COG id: COG0834

COG function: function code ET; ABC-type amino acid transport/signal transduction systems, periplasmic component/domain

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1788649, Length=261, Percent_Identity=41.3793103448276, Blast_Score=197, Evalue=7e-52,
Organism=Escherichia coli, GI1787085, Length=250, Percent_Identity=39.6, Blast_Score=182, Evalue=2e-47,
Organism=Escherichia coli, GI1788648, Length=234, Percent_Identity=37.6068376068376, Blast_Score=175, Evalue=3e-45,
Organism=Escherichia coli, GI1787088, Length=249, Percent_Identity=36.5461847389558, Blast_Score=171, Evalue=3e-44,
Organism=Escherichia coli, GI1788228, Length=237, Percent_Identity=32.9113924050633, Blast_Score=122, Evalue=3e-29,
Organism=Escherichia coli, GI1787031, Length=253, Percent_Identity=31.2252964426877, Blast_Score=103, Evalue=1e-23,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005768
- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 28109; Mature: 28109

Theoretical pI: Translated: 5.21; Mature: 5.21

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKWLLVAALAATAATGVAQAKEWKTVRFGIEGAYPPFSWTEADGSLKGFDVDMANALCT
CCHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHH
EMQVKCKIVAQDWDGIIPSLLARKYDAIIAAMSITEERKKKIDFTGKYALIPNKFIAKKG
HHEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCHHHHHCC
AGLNFDDLSGQKIAVQRATTHDKYLTDNYGDTVEIVRYGSFDEAYLDLANGRVAAVLGDA
CCCCCCCCCCCEEEEEECCCCCCEECCCCCCEEEEEEECCCCHHHHHHCCCEEEEEECCH
SALEEGVLNKAGGEDYEFVGPSLTDPKWFGEGFGIALRKQDKDLTKQLDAAILSLREKGI
HHHHHHHHHCCCCCCCEECCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHCCH
YQDIAAKYFNYDVYGK
HHHHHHHHHCCCCCCC
>Mature Secondary Structure
MKKWLLVAALAATAATGVAQAKEWKTVRFGIEGAYPPFSWTEADGSLKGFDVDMANALCT
CCHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHH
EMQVKCKIVAQDWDGIIPSLLARKYDAIIAAMSITEERKKKIDFTGKYALIPNKFIAKKG
HHEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCHHHHHCC
AGLNFDDLSGQKIAVQRATTHDKYLTDNYGDTVEIVRYGSFDEAYLDLANGRVAAVLGDA
CCCCCCCCCCCEEEEEECCCCCCEECCCCCCEEEEEEECCCCHHHHHHCCCEEEEEECCH
SALEEGVLNKAGGEDYEFVGPSLTDPKWFGEGFGIALRKQDKDLTKQLDAAILSLREKGI
HHHHHHHHHCCCCCCCEECCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHCCH
YQDIAAKYFNYDVYGK
HHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-ornithine [Periplasm]; H2O; ATP; L-arginine [Periplasm]; L-lysine [Periplasm] [C]

Specific reaction: ATP + L-ornithine [Periplasm] + H2O = ADP + phosphate + L-ornithine [Cytoplasm] ATP + L-arginine [Periplasm] + H2O = ADP + phosphate + L-arginine [Cytoplasm] ATP + L-lysine [Periplasm] + H2O = ADP + phosphate + L-lysine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503; 3040734; 9298646 [H]