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The map label for this gene is pykA [H]

Identifier: 218709031

GI number: 218709031

Start: 1078987

End: 1080435

Strand: Reverse

Name: pykA [H]

Synonym: VS_1037

Alternate gene names: 218709031

Gene position: 1080435-1078987 (Counterclockwise)

Preceding gene: 218709034

Following gene: 218709029

Centisome position: 32.75

GC content: 48.03

Gene sequence:

>1449_bases
ATGACAGCCGAATTAAGAAAAACGAAAATCGTCACAACGCTAGGCCCTTCTACTGATAAAGGTAACGTGCTTGAAGAGAT
CATCAAAGCCGGTGCCAATGTCGTCCGTATGAACTTCTCTCACGGCACTAGCGACGACCATATACAACGTGCAGAAAATG
TCCGCGCAATAGCCAAAAAACTTGGTACTCAGATCGCTATTCTCGGTGACTTACAAGGTCCCAAGATTCGAGTGTCAACA
TTCAAAGATGGCAAAATCCAACTTGCCGTTGGTGACCAATTTACTCTCGACAGTAGCCTTGGTTTAGGCGAAGGCAACCA
ACAAGCGGTCGGTCTTGATTATAAAGAATTACCTAACGACGTTTCAGCTAACGACATCCTGTTGCTAGACGATGGTCGTG
TACAGCTAAAAGTAACCGAAGTGGAAGGTTGTCGCGTTCATACCGAAGTCATCATTGGCGGCCCTCTCTCGAATAATAAA
GGCATAAACAAAAAAGGCGGCGGCTTGTCAGCAGAAGCGCTGACAGAAAAAGACAAACGCGACATCGTCACCGCCGCACA
AATCAAAGTGGATTACCTTGCGGTGTCTTTCCCACGCAACGGCGAAGACATGCACTACGCTCGTCAACTCGCACGAGAAG
CAGGTTTAGAAGCTCACCTTGTAGCAAAAGTAGAGCGAGCAGAGACGGTCGCCACTGTTGAGAACATGGATGACATCATA
ATGGCCTCAGATGTGGTCATGGTAGCTCGTGGTGACCTCGGAGTCGAAATTGGCGACCCTGAATTGGTCGGAGTACAAAA
ACAGCTTATACGTCGTGCTAGAGCGCTTAACCGAACGGTGATCACCGCAACTCAAATGATGGAATCGATGATTTCAGCGC
CAATGCCAACCCGTGCCGAAGTCATGGACGTTGCCAATGCGGTACTTGATGGAACAGATGCGGTCATGCTTTCAGGTGAA
ACAGCTGCAGGTGATTACCCGGTCGAAACCGTAAAATCGATGGCTGAAGTGTGTATTGGTGCTGAGAAAATGGCGGGGAT
TAACAACCAATCCAATTACCGAATTGACCGCACGTTTGTGACCGCCGAAGAAACCGTTTCGATGGCGACTATCTACTCGG
CAAACCATATGGAAGGCATTAAAGGCGTAGTCACTCTCACTGAATCAGGCCGTACTGCACTGATGATGTCTCGTCTAAGT
GCAGATATGCCTATCTACGCACTATCTCGTAACGAAGGAACACTTAATCGCTGCACCTTGTACCGAGGTGTAACACCAAT
CTACTTCGAGACAGAAGCCAAAGACAGTTTTGACATTGCCCTATCTACGCTCACCTGCCTAAAAGAGCAAGAGCATCTTA
AGTTTGGCGATCTCGTTATCGTCACTCAAGGCGACATTATGGATGTGGCGGGATCAACCAACTGTATGAGAATCTTGCCT
GTCACCTAA

Upstream 100 bases:

>100_bases
CCTTGTATAAATACCCAGCAACTCGCTGCAAAAATCAGCTCGAAAGATCAACACGCCCTAGTCTTTTAAAAGATTACTTG
GCCTCACTCTAGGAGTATTC

Downstream 100 bases:

>100_bases
TGTTTGCCCGTAAGGCGAAACGATTTGCCTAATCTCATGACAAAAAAGGGTTATCGAACCAAGTTCAATAACCCTTTTCA
TTTCAGAACACGACTCACTA

Product: pyruvate kinase II

Products: NA

Alternate protein names: PK-2 [H]

Number of amino acids: Translated: 482; Mature: 481

Protein sequence:

>482_residues
MTAELRKTKIVTTLGPSTDKGNVLEEIIKAGANVVRMNFSHGTSDDHIQRAENVRAIAKKLGTQIAILGDLQGPKIRVST
FKDGKIQLAVGDQFTLDSSLGLGEGNQQAVGLDYKELPNDVSANDILLLDDGRVQLKVTEVEGCRVHTEVIIGGPLSNNK
GINKKGGGLSAEALTEKDKRDIVTAAQIKVDYLAVSFPRNGEDMHYARQLAREAGLEAHLVAKVERAETVATVENMDDII
MASDVVMVARGDLGVEIGDPELVGVQKQLIRRARALNRTVITATQMMESMISAPMPTRAEVMDVANAVLDGTDAVMLSGE
TAAGDYPVETVKSMAEVCIGAEKMAGINNQSNYRIDRTFVTAEETVSMATIYSANHMEGIKGVVTLTESGRTALMMSRLS
ADMPIYALSRNEGTLNRCTLYRGVTPIYFETEAKDSFDIALSTLTCLKEQEHLKFGDLVIVTQGDIMDVAGSTNCMRILP
VT

Sequences:

>Translated_482_residues
MTAELRKTKIVTTLGPSTDKGNVLEEIIKAGANVVRMNFSHGTSDDHIQRAENVRAIAKKLGTQIAILGDLQGPKIRVST
FKDGKIQLAVGDQFTLDSSLGLGEGNQQAVGLDYKELPNDVSANDILLLDDGRVQLKVTEVEGCRVHTEVIIGGPLSNNK
GINKKGGGLSAEALTEKDKRDIVTAAQIKVDYLAVSFPRNGEDMHYARQLAREAGLEAHLVAKVERAETVATVENMDDII
MASDVVMVARGDLGVEIGDPELVGVQKQLIRRARALNRTVITATQMMESMISAPMPTRAEVMDVANAVLDGTDAVMLSGE
TAAGDYPVETVKSMAEVCIGAEKMAGINNQSNYRIDRTFVTAEETVSMATIYSANHMEGIKGVVTLTESGRTALMMSRLS
ADMPIYALSRNEGTLNRCTLYRGVTPIYFETEAKDSFDIALSTLTCLKEQEHLKFGDLVIVTQGDIMDVAGSTNCMRILP
VT
>Mature_481_residues
TAELRKTKIVTTLGPSTDKGNVLEEIIKAGANVVRMNFSHGTSDDHIQRAENVRAIAKKLGTQIAILGDLQGPKIRVSTF
KDGKIQLAVGDQFTLDSSLGLGEGNQQAVGLDYKELPNDVSANDILLLDDGRVQLKVTEVEGCRVHTEVIIGGPLSNNKG
INKKGGGLSAEALTEKDKRDIVTAAQIKVDYLAVSFPRNGEDMHYARQLAREAGLEAHLVAKVERAETVATVENMDDIIM
ASDVVMVARGDLGVEIGDPELVGVQKQLIRRARALNRTVITATQMMESMISAPMPTRAEVMDVANAVLDGTDAVMLSGET
AAGDYPVETVKSMAEVCIGAEKMAGINNQSNYRIDRTFVTAEETVSMATIYSANHMEGIKGVVTLTESGRTALMMSRLSA
DMPIYALSRNEGTLNRCTLYRGVTPIYFETEAKDSFDIALSTLTCLKEQEHLKFGDLVIVTQGDIMDVAGSTNCMRILPV
T

Specific function: Glycolysis; final step. [C]

COG id: COG0469

COG function: function code G; Pyruvate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pyruvate kinase family [H]

Homologues:

Organism=Homo sapiens, GI33286418, Length=495, Percent_Identity=35.7575757575758, Blast_Score=269, Evalue=5e-72,
Organism=Homo sapiens, GI33286422, Length=496, Percent_Identity=35.4838709677419, Blast_Score=269, Evalue=5e-72,
Organism=Homo sapiens, GI33286420, Length=496, Percent_Identity=35.4838709677419, Blast_Score=269, Evalue=5e-72,
Organism=Homo sapiens, GI32967597, Length=500, Percent_Identity=34.6, Blast_Score=241, Evalue=8e-64,
Organism=Homo sapiens, GI10835121, Length=502, Percent_Identity=34.6613545816733, Blast_Score=241, Evalue=1e-63,
Organism=Homo sapiens, GI310128732, Length=290, Percent_Identity=33.7931034482759, Blast_Score=153, Evalue=3e-37,
Organism=Homo sapiens, GI310128730, Length=290, Percent_Identity=33.7931034482759, Blast_Score=153, Evalue=3e-37,
Organism=Homo sapiens, GI310128736, Length=236, Percent_Identity=31.7796610169492, Blast_Score=118, Evalue=1e-26,
Organism=Homo sapiens, GI310128734, Length=236, Percent_Identity=31.7796610169492, Blast_Score=118, Evalue=1e-26,
Organism=Homo sapiens, GI310128738, Length=215, Percent_Identity=31.1627906976744, Blast_Score=102, Evalue=8e-22,
Organism=Escherichia coli, GI1788160, Length=481, Percent_Identity=67.983367983368, Blast_Score=660, Evalue=0.0,
Organism=Escherichia coli, GI1787965, Length=478, Percent_Identity=37.6569037656904, Blast_Score=293, Evalue=2e-80,
Organism=Caenorhabditis elegans, GI17544584, Length=490, Percent_Identity=35.7142857142857, Blast_Score=270, Evalue=2e-72,
Organism=Caenorhabditis elegans, GI17506831, Length=491, Percent_Identity=34.826883910387, Blast_Score=240, Evalue=9e-64,
Organism=Caenorhabditis elegans, GI71984413, Length=491, Percent_Identity=34.826883910387, Blast_Score=240, Evalue=1e-63,
Organism=Caenorhabditis elegans, GI71984406, Length=491, Percent_Identity=34.826883910387, Blast_Score=240, Evalue=1e-63,
Organism=Caenorhabditis elegans, GI17506829, Length=491, Percent_Identity=34.826883910387, Blast_Score=240, Evalue=1e-63,
Organism=Saccharomyces cerevisiae, GI6319279, Length=493, Percent_Identity=35.2941176470588, Blast_Score=253, Evalue=4e-68,
Organism=Saccharomyces cerevisiae, GI6324923, Length=441, Percent_Identity=34.6938775510204, Blast_Score=235, Evalue=1e-62,
Organism=Drosophila melanogaster, GI28571814, Length=501, Percent_Identity=36.5269461077844, Blast_Score=259, Evalue=2e-69,
Organism=Drosophila melanogaster, GI24648964, Length=501, Percent_Identity=36.5269461077844, Blast_Score=259, Evalue=3e-69,
Organism=Drosophila melanogaster, GI24648966, Length=419, Percent_Identity=36.9928400954654, Blast_Score=243, Evalue=2e-64,
Organism=Drosophila melanogaster, GI24581235, Length=488, Percent_Identity=29.5081967213115, Blast_Score=211, Evalue=1e-54,
Organism=Drosophila melanogaster, GI24646914, Length=270, Percent_Identity=34.4444444444444, Blast_Score=147, Evalue=2e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001697
- InterPro:   IPR015813
- InterPro:   IPR011037
- InterPro:   IPR015794
- InterPro:   IPR018209
- InterPro:   IPR015793
- InterPro:   IPR015795
- InterPro:   IPR015806 [H]

Pfam domain/function: PF00224 PK; PF02887 PK_C [H]

EC number: =2.7.1.40 [H]

Molecular weight: Translated: 52038; Mature: 51907

Theoretical pI: Translated: 4.87; Mature: 4.87

Prosite motif: PS00110 PYRUVATE_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAELRKTKIVTTLGPSTDKGNVLEEIIKAGANVVRMNFSHGTSDDHIQRAENVRAIAKK
CCCCCCCEEEEEEECCCCCCCHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHH
LGTQIAILGDLQGPKIRVSTFKDGKIQLAVGDQFTLDSSLGLGEGNQQAVGLDYKELPND
HCCEEEEEECCCCCEEEEEEECCCEEEEEECCCEEECCCCCCCCCCCCEECCCHHHCCCC
VSANDILLLDDGRVQLKVTEVEGCRVHTEVIIGGPLSNNKGINKKGGGLSAEALTEKDKR
CCCCEEEEEECCEEEEEEEEECCCEEEEEEEEECCCCCCCCCCCCCCCCCHHHHCCCHHH
DIVTAAQIKVDYLAVSFPRNGEDMHYARQLAREAGLEAHLVAKVERAETVATVENMDDII
CCEEEEEEEEEEEEEECCCCCCHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHCCCCCE
MASDVVMVARGDLGVEIGDPELVGVQKQLIRRARALNRTVITATQMMESMISAPMPTRAE
EECCEEEEEECCCCEEECCCCEECHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCCCHHH
VMDVANAVLDGTDAVMLSGETAAGDYPVETVKSMAEVCIGAEKMAGINNQSNYRIDRTFV
HHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHCHHHHCCCCCCCCEEEEEEEE
TAEETVSMATIYSANHMEGIKGVVTLTESGRTALMMSRLSADMPIYALSRNEGTLNRCTL
EHHHHEEEEEEECCCCCCCCCEEEEEECCCCCEEHHHHHCCCCCEEEEECCCCCCCEEEE
YRGVTPIYFETEAKDSFDIALSTLTCLKEQEHLKFGDLVIVTQGDIMDVAGSTNCMRILP
ECCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEECCCEEECCCCCCEEEEEE
VT
CC
>Mature Secondary Structure 
TAELRKTKIVTTLGPSTDKGNVLEEIIKAGANVVRMNFSHGTSDDHIQRAENVRAIAKK
CCCCCCEEEEEEECCCCCCCHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHH
LGTQIAILGDLQGPKIRVSTFKDGKIQLAVGDQFTLDSSLGLGEGNQQAVGLDYKELPND
HCCEEEEEECCCCCEEEEEEECCCEEEEEECCCEEECCCCCCCCCCCCEECCCHHHCCCC
VSANDILLLDDGRVQLKVTEVEGCRVHTEVIIGGPLSNNKGINKKGGGLSAEALTEKDKR
CCCCEEEEEECCEEEEEEEEECCCEEEEEEEEECCCCCCCCCCCCCCCCCHHHHCCCHHH
DIVTAAQIKVDYLAVSFPRNGEDMHYARQLAREAGLEAHLVAKVERAETVATVENMDDII
CCEEEEEEEEEEEEEECCCCCCHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHCCCCCE
MASDVVMVARGDLGVEIGDPELVGVQKQLIRRARALNRTVITATQMMESMISAPMPTRAE
EECCEEEEEECCCCEEECCCCEECHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCCCHHH
VMDVANAVLDGTDAVMLSGETAAGDYPVETVKSMAEVCIGAEKMAGINNQSNYRIDRTFV
HHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHCHHHHCCCCCCCCEEEEEEEE
TAEETVSMATIYSANHMEGIKGVVTLTESGRTALMMSRLSADMPIYALSRNEGTLNRCTL
EHHHHEEEEEEECCCCCCCCCEEEEEECCCCCEEHHHHHCCCCCEEEEECCCCCCCEEEE
YRGVTPIYFETEAKDSFDIALSTLTCLKEQEHLKFGDLVIVTQGDIMDVAGSTNCMRILP
ECCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEECCCEEECCCCCCEEEEEE
VT
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097040; 9278503; 1732206; 1859631; 8439398 [H]