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The map label for this gene is mraW

Identifier: 218708478

GI number: 218708478

Start: 451541

End: 452491

Strand: Direct

Name: mraW

Synonym: VS_0442

Alternate gene names: 218708478

Gene position: 451541-452491 (Clockwise)

Preceding gene: 218708477

Following gene: 218708479

Centisome position: 13.69

GC content: 46.06

Gene sequence:

>951_bases
ATGACAGAAGCATTCAAACATATTTCAGTATTGCTTAACGAATCTATTGACGGACTTGCGATCAAACCTGACGGTACCTA
TATCGATGGTACTTTTGGCCGTGGTGGTCACAGCCGTACAATCCTGTCTAAACTGGGCGAGAATGGAAGACTCTTTAGTA
TCGACCGCGATCCACAAGCGATTGCCGAAGCACAAAAGATTGATGACCCTCGTTTTACGATTATTCACGGCCCGTTCTCA
GGTATGGCTGAATATGCAGAGCGTTATGACTTAGTCGGTCAAGTGGATGGCGTATTGCTTGATTTAGGTGTCTCTTCACC
ACAGCTGGATGATGCTGAACGTGGCTTTAGCTTTATGAAAGATGGCCCGCTTGATATGCGTATGGATCCAACAACCGGCA
TTCCTGTTTCTCAGTGGTTGGTTGAAGCTGATCTTGATGACATCACATGGGTTATTCGTGAGTTCGGTGAAGACAAACAC
GCTCGTCGTATCGCGAAAGGCATCATTGCTTATCAAGAGAATGAAGAGAACGAACCGCTAACGCGCACTGGACAGTTGGC
TAAGCTTATCTCTGATGTCGCTCCAAAAAGCTTCAAAGAGAAAAAACACCCAGCAACACGTGCTTTCCAAGCATTCCGTA
TCTACATTAACAGTGAACTAGAAGAGATCGATACAGCACTGAAAGGCGCGGCAAGCATTCTAGCTCCTCAAGGTCGTATC
TCTGTTATCAGTTTCCACTCGCTTGAAGACCGTATGGTGAAGCGCTTTATCCGTAAAGAGAGCCAAGGACCGCAAGTACC
ACATGGCCTGCCACTAACTGAAGAGCAGATCAAAGCGCTAGGCAGTGCCGATCTGAAACCAATTGGTAAAGCAATTAAGC
CTTCTAAAGATGAAGTGGATGAGAATACTCGTTCGCGTAGTTCAGTACTACGAATCGCAGAAAAGCTATAG

Upstream 100 bases:

>100_bases
ACACAATATGGTGGAGATACGGCGTGAAATCAGTAAACTAAAACGTTAATAGAACATATTATTGCCGAACTAATGGCTCA
ATCCATTCACTGAGAAGACT

Downstream 100 bases:

>100_bases
TCAATGAAGACCTCCAAACCCAACTTAGCCAAGATAATCTTTTTTGATTTGATCTCCGTGGGCAAAGTCCCATTGGGGCT
TCTTATCTGCATCTTTGCGA

Product: S-adenosyl-methyltransferase MraW

Products: NA

Alternate protein names: 16S rRNA m(4)C1402 methyltransferase; rRNA (cytosine-N(4)-)-methyltransferase RsmH

Number of amino acids: Translated: 316; Mature: 315

Protein sequence:

>316_residues
MTEAFKHISVLLNESIDGLAIKPDGTYIDGTFGRGGHSRTILSKLGENGRLFSIDRDPQAIAEAQKIDDPRFTIIHGPFS
GMAEYAERYDLVGQVDGVLLDLGVSSPQLDDAERGFSFMKDGPLDMRMDPTTGIPVSQWLVEADLDDITWVIREFGEDKH
ARRIAKGIIAYQENEENEPLTRTGQLAKLISDVAPKSFKEKKHPATRAFQAFRIYINSELEEIDTALKGAASILAPQGRI
SVISFHSLEDRMVKRFIRKESQGPQVPHGLPLTEEQIKALGSADLKPIGKAIKPSKDEVDENTRSRSSVLRIAEKL

Sequences:

>Translated_316_residues
MTEAFKHISVLLNESIDGLAIKPDGTYIDGTFGRGGHSRTILSKLGENGRLFSIDRDPQAIAEAQKIDDPRFTIIHGPFS
GMAEYAERYDLVGQVDGVLLDLGVSSPQLDDAERGFSFMKDGPLDMRMDPTTGIPVSQWLVEADLDDITWVIREFGEDKH
ARRIAKGIIAYQENEENEPLTRTGQLAKLISDVAPKSFKEKKHPATRAFQAFRIYINSELEEIDTALKGAASILAPQGRI
SVISFHSLEDRMVKRFIRKESQGPQVPHGLPLTEEQIKALGSADLKPIGKAIKPSKDEVDENTRSRSSVLRIAEKL
>Mature_315_residues
TEAFKHISVLLNESIDGLAIKPDGTYIDGTFGRGGHSRTILSKLGENGRLFSIDRDPQAIAEAQKIDDPRFTIIHGPFSG
MAEYAERYDLVGQVDGVLLDLGVSSPQLDDAERGFSFMKDGPLDMRMDPTTGIPVSQWLVEADLDDITWVIREFGEDKHA
RRIAKGIIAYQENEENEPLTRTGQLAKLISDVAPKSFKEKKHPATRAFQAFRIYINSELEEIDTALKGAASILAPQGRIS
VISFHSLEDRMVKRFIRKESQGPQVPHGLPLTEEQIKALGSADLKPIGKAIKPSKDEVDENTRSRSSVLRIAEKL

Specific function: Specifically methylates the N4 position of cytidine in position 1402 (C1402) of 16S rRNA

COG id: COG0275

COG function: function code M; Predicted S-adenosylmethionine-dependent methyltransferase involved in cell envelope biogenesis

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmH family

Homologues:

Organism=Homo sapiens, GI165377209, Length=347, Percent_Identity=37.1757925072046, Blast_Score=187, Evalue=1e-47,
Organism=Homo sapiens, GI165377202, Length=204, Percent_Identity=37.7450980392157, Blast_Score=122, Evalue=4e-28,
Organism=Escherichia coli, GI1786270, Length=316, Percent_Identity=62.6582278481013, Blast_Score=415, Evalue=1e-117,
Organism=Drosophila melanogaster, GI62472493, Length=332, Percent_Identity=37.0481927710843, Blast_Score=185, Evalue=3e-47,
Organism=Drosophila melanogaster, GI28571637, Length=332, Percent_Identity=37.0481927710843, Blast_Score=185, Evalue=3e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMH_VIBSL (B7VIZ5)

Other databases:

- EMBL:   FM954972
- RefSeq:   YP_002416099.1
- ProteinModelPortal:   B7VIZ5
- SMR:   B7VIZ5
- GeneID:   7162767
- GenomeReviews:   FM954972_GR
- KEGG:   vsp:VS_0442
- HOGENOM:   HBG302779
- OMA:   PQLDDPE
- ProtClustDB:   PRK00050
- GO:   GO:0005737
- HAMAP:   MF_01007
- InterPro:   IPR002903
- PANTHER:   PTHR11265
- PIRSF:   PIRSF004486
- TIGRFAMs:   TIGR00006

Pfam domain/function: PF01795 Methyltransf_5

EC number: NA

Molecular weight: Translated: 35050; Mature: 34919

Theoretical pI: Translated: 5.49; Mature: 5.49

Prosite motif: NA

Important sites: BINDING 55-55 BINDING 79-79 BINDING 101-101 BINDING 108-108

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEAFKHISVLLNESIDGLAIKPDGTYIDGTFGRGGHSRTILSKLGENGRLFSIDRDPQA
CCHHHHHHHHHHHCCCCCEEECCCCCEECCCCCCCCCHHHHHHHCCCCCCEEEECCCHHH
IAEAQKIDDPRFTIIHGPFSGMAEYAERYDLVGQVDGVLLDLGVSSPQLDDAERGFSFMK
HHHHHHCCCCCEEEEECCHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCCHHHCCHHHC
DGPLDMRMDPTTGIPVSQWLVEADLDDITWVIREFGEDKHARRIAKGIIAYQENEENEPL
CCCCCEEECCCCCCCHHHHHHHCCHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCH
TRTGQLAKLISDVAPKSFKEKKHPATRAFQAFRIYINSELEEIDTALKGAASILAPQGRI
HHHHHHHHHHHHHCCHHHHHHCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCE
SVISFHSLEDRMVKRFIRKESQGPQVPHGLPLTEEQIKALGSADLKPIGKAIKPSKDEVD
EEEEEHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHCCCCCHHHHHHCCCCHHHHH
ENTRSRSSVLRIAEKL
HHHHHHHHHHHHHHHC
>Mature Secondary Structure 
TEAFKHISVLLNESIDGLAIKPDGTYIDGTFGRGGHSRTILSKLGENGRLFSIDRDPQA
CHHHHHHHHHHHCCCCCEEECCCCCEECCCCCCCCCHHHHHHHCCCCCCEEEECCCHHH
IAEAQKIDDPRFTIIHGPFSGMAEYAERYDLVGQVDGVLLDLGVSSPQLDDAERGFSFMK
HHHHHHCCCCCEEEEECCHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCCHHHCCHHHC
DGPLDMRMDPTTGIPVSQWLVEADLDDITWVIREFGEDKHARRIAKGIIAYQENEENEPL
CCCCCEEECCCCCCCHHHHHHHCCHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCH
TRTGQLAKLISDVAPKSFKEKKHPATRAFQAFRIYINSELEEIDTALKGAASILAPQGRI
HHHHHHHHHHHHHCCHHHHHHCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCE
SVISFHSLEDRMVKRFIRKESQGPQVPHGLPLTEEQIKALGSADLKPIGKAIKPSKDEVD
EEEEEHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHCCCCCHHHHHHCCCCHHHHH
ENTRSRSSVLRIAEKL
HHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA