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The map label for this gene is nudF [H]

Identifier: 218708456

GI number: 218708456

Start: 429385

End: 430014

Strand: Direct

Name: nudF [H]

Synonym: VS_0419

Alternate gene names: 218708456

Gene position: 429385-430014 (Clockwise)

Preceding gene: 218708454

Following gene: 218708457

Centisome position: 13.01

GC content: 46.03

Gene sequence:

>630_bases
ATGCAACAGTATGACAATCAACGACATGAGTTTACTCCGCAAGATGTGGAAATAGTCTCAAAAGAGACGCTGTTTCGTGG
TTTTTTCAAAATGGTTAAGTACACATTTAAACATAGACTGTTTGAGGGCGGCTGGAGCCAACCAATCGAGCGTGAGATGT
TTGAGCGTGGACATGCTGCCGCGTTGTTACCTTATGATCCTATACGTGACGAAGTCGTGATCGTCGAACAGATCCGTGTC
GGTGCTCTAGAGCATGAGAACCCGTGGCAATACGAAATTGTTGCGGGGATTATTGATACCGATGAATCACCACAAGATGT
TGCTCGTCGTGAGGCGATGGAAGAAGCGGGAGTCGAAGTCGGATCTGTATTGCCTATTACTTCGTATTATCCTTCATCCG
GCGGTTGCTCAGAAAAGCTCGACGTCTTTGTTGGTTGTGTTGACGCCACGACAGCAAAAGGGGTTCACGGATTGGATTAC
GAAGGTGAAGATATTCGAGTACAAGTGATGAGTCGCGAAGCCGCTTATCAGTTAGTAAAAGACGGTGTGTTTGAAAATGG
CGCCACGATCATTGCGCTGCAGTGGCTACAATTAAACTACCAAGAATTACAGTCAGAGTGGATAGATTAA

Upstream 100 bases:

>100_bases
CTTAACTTTAATTATAAGTTTTATACTAAAAGTTGAACCTTATCCTTGGTAGTTAACTCGGACAATGAGTAAACTATAAG
ATCACTTAGTGAGGTATCAA

Downstream 100 bases:

>100_bases
CGTCATGCCAAATATAGCAGTCAAAAAACCGTATCATGTCGATCTGGCTGAATTGATGCGAGTTTACGAGACTAACTATG
CCAAACTTAATGCTCTGTTA

Product: MutT/nudix family protein

Products: NA

Alternate protein names: ADP-ribose diphosphatase; ADP-ribose phosphohydrolase; ASPPase; Adenosine diphosphoribose pyrophosphatase; ADPR-PPase [H]

Number of amino acids: Translated: 209; Mature: 209

Protein sequence:

>209_residues
MQQYDNQRHEFTPQDVEIVSKETLFRGFFKMVKYTFKHRLFEGGWSQPIEREMFERGHAAALLPYDPIRDEVVIVEQIRV
GALEHENPWQYEIVAGIIDTDESPQDVARREAMEEAGVEVGSVLPITSYYPSSGGCSEKLDVFVGCVDATTAKGVHGLDY
EGEDIRVQVMSREAAYQLVKDGVFENGATIIALQWLQLNYQELQSEWID

Sequences:

>Translated_209_residues
MQQYDNQRHEFTPQDVEIVSKETLFRGFFKMVKYTFKHRLFEGGWSQPIEREMFERGHAAALLPYDPIRDEVVIVEQIRV
GALEHENPWQYEIVAGIIDTDESPQDVARREAMEEAGVEVGSVLPITSYYPSSGGCSEKLDVFVGCVDATTAKGVHGLDY
EGEDIRVQVMSREAAYQLVKDGVFENGATIIALQWLQLNYQELQSEWID
>Mature_209_residues
MQQYDNQRHEFTPQDVEIVSKETLFRGFFKMVKYTFKHRLFEGGWSQPIEREMFERGHAAALLPYDPIRDEVVIVEQIRV
GALEHENPWQYEIVAGIIDTDESPQDVARREAMEEAGVEVGSVLPITSYYPSSGGCSEKLDVFVGCVDATTAKGVHGLDY
EGEDIRVQVMSREAAYQLVKDGVFENGATIIALQWLQLNYQELQSEWID

Specific function: Acts on ADP-mannose and ADP-glucose as well as ADP- ribose. Prevents glycogen biosynthesis. The reaction catalyzed by this enzyme is a limiting step of the gluconeogenic process [H]

COG id: COG0494

COG function: function code LR; NTP pyrophosphohydrolases including oxidative damage repair enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 nudix hydrolase domain [H]

Homologues:

Organism=Escherichia coli, GI1789412, Length=208, Percent_Identity=56.25, Blast_Score=241, Evalue=3e-65,
Organism=Escherichia coli, GI1788810, Length=191, Percent_Identity=29.8429319371728, Blast_Score=82, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004385
- InterPro:   IPR020084
- InterPro:   IPR000086
- InterPro:   IPR015797 [H]

Pfam domain/function: PF00293 NUDIX [H]

EC number: =3.6.1.13 [H]

Molecular weight: Translated: 23854; Mature: 23854

Theoretical pI: Translated: 4.26; Mature: 4.26

Prosite motif: PS00893 NUDIX

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQQYDNQRHEFTPQDVEIVSKETLFRGFFKMVKYTFKHRLFEGGWSQPIEREMFERGHAA
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCE
ALLPYDPIRDEVVIVEQIRVGALEHENPWQYEIVAGIIDTDESPQDVARREAMEEAGVEV
EECCCCCCCCCEEEEEHHHHCCCCCCCCCEEEEEEEEECCCCCHHHHHHHHHHHHHCCCC
GSVLPITSYYPSSGGCSEKLDVFVGCVDATTAKGVHGLDYEGEDIRVQVMSREAAYQLVK
CCCCEEECCCCCCCCCCHHHHHEEEECCCCCCCCCCCCCCCCCCEEEEEECHHHHHHHHH
DGVFENGATIIALQWLQLNYQELQSEWID
CCHHCCCCEEEEEEEHHCCHHHHHHHHCC
>Mature Secondary Structure
MQQYDNQRHEFTPQDVEIVSKETLFRGFFKMVKYTFKHRLFEGGWSQPIEREMFERGHAA
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCE
ALLPYDPIRDEVVIVEQIRVGALEHENPWQYEIVAGIIDTDESPQDVARREAMEEAGVEV
EECCCCCCCCCEEEEEHHHHCCCCCCCCCEEEEEEEEECCCCCHHHHHHHHHHHHHCCCC
GSVLPITSYYPSSGGCSEKLDVFVGCVDATTAKGVHGLDYEGEDIRVQVMSREAAYQLVK
CCCCEEECCCCCCCCCCHHHHHEEEECCCCCCCCCCCCCCCCCCEEEEEECHHHHHHHHH
DGVFENGATIIALQWLQLNYQELQSEWID
CCHHCCCCEEEEEEEHHCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]