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The map label for this gene is hldE

Identifier: 218708454

GI number: 218708454

Start: 426236

End: 427666

Strand: Direct

Name: hldE

Synonym: VS_0417

Alternate gene names: 218708454

Gene position: 426236-427666 (Clockwise)

Preceding gene: 218708453

Following gene: 218708456

Centisome position: 12.92

GC content: 46.96

Gene sequence:

>1431_bases
ATGAAACCAATTCTACCTGACTACAGCCAATCAGGCGTTCTTGTTGTCGGTGATGTAATGCTTGACCGTTACTGGTATGG
CCCTACTGGCCGTATTTCACCAGAAGCACCTGTGCCCGTTGTTAAAGTAGAAAATAACGAAGAGCGCCCTGGTGGTGCTG
CCAACGTAGCAATGAATATTGCCTCTCTTGGTGGTCATGCTCATATCGTTGGTTTAACTGGAAAAGATGAGCCAGCTGAG
GTATTGAAAAATACCTTGGGTGCATTAAAGGTTAAGTGTGATTTTGTTGAGTTGGACGATTACCCGACCATCACAAAATT
GCGTGTCATGAGCCGTGGACAGCAGTTGATCCGTCTTGATTTTGAAGACAAATTTGAGAACACGGATCCTGAGCTTGTTT
TATCTCGCATGGAGCAAGCACTGCCTAATGTACGCTCGGTAATCTTATCTGATTATGCGAAAGGTGCTTTGGAGCATGTG
CAGAGCTTTATTCAAAAAGCGCGCGCAGCGAACGTTCCGGTGTTTATTGATCCTAAAGGTGCAGATTTTGAACGCTACCG
TGGGGCGACATTACTTACGCCAAACATGGCGGAGTTTGAGCTGGTAGCCGGTAAGGTTAAGTCTGAAGATGAAATGATCG
AAAAAGGGCTCGCGTTAATTGAAGAGTTTGATTTTGAAGCCTTGCTCGTGACTCGCAGTGAGCATGGCATGACATTGCTC
CGCAAAGGCGTAGAGCCATTCCACTTACCGACCCAGGCGAAAGAAGTGTATGACGTTACTGGTGCTGGTGATACGGTTAT
TTCAGTATTAGCAGCTTCAGTTGCTGCAGGTAAGCCACTTGATGAAGCGTGTGCATTAGCAAACGCTGCAGCTGGCGTGG
TAGTCGGCAAGCTAGGTACATCGACGTTGTCTACGATTGAGTTAGCGGAAGCGATTCATGGTAGCCAAGATACTGACTAC
GGTGTGATCTCTGAAGCTGCCCTAGTTGAAGCGGTGAAACGTGCTCGTGCGAAAGGCGAGAAAGTCGTTATGACTAATGG
CTGCTTTGATATTCTGCATGCGGGACATGTCTCTTACATGAACCATGCTGCTGAGTTGGGTGATCGTTTGATCGTTGCTG
TGAATACGGATGAATCAGTGAAACGCTTAAAAGGTCCAGGTCGTCCTGTGAACCCAACTGATCGTCGTATGGCGGTACTG
GCTGGTTTAGGTGCGGTTGATTGGGTTGTTCCGTTTTCTGAAGATACACCGCAACGTTTGATCTCTGAAGTTCTTCCAAG
CATTCTTGTGAAAGGTGGTGATTACAAACCTGAAGAGATTGCTGGTGGTGCGGAAGTGATTGCGGCTGGCGGTGAAGTTA
AAGTACTTAACTTTGAAGATGGTTGCTCTACGACTGAAATCATTAAAGCGATTAAAGGTGGTCGAGGCTAA

Upstream 100 bases:

>100_bases
TGAGTATCTATCGAGTTAGGCTTTCACTCATTTTTCTATGGTTTAGCCACTGTAATCAGTGTGCTAGACTCTAGCCAGAT
TCGATTTTTGGAGATCCACA

Downstream 100 bases:

>100_bases
CTCTTAGTCTTCTAGCTTTCTCGTCTCAAGGCTTAACCTTTGTGTTAGCTGAGAATTAGCTTTCAATAGATAATAAAAAT
GCCGCTCATTGAGCGGCATT

Product: bifunctional heptose 7-phosphate kinase/heptose 1-phosphate adenyltransferase

Products: NA

Alternate protein names: D-beta-D-heptose 7-phosphate kinase; D-beta-D-heptose 7-phosphotransferase; D-beta-D-heptose 1-phosphate adenosyltransferase

Number of amino acids: Translated: 476; Mature: 476

Protein sequence:

>476_residues
MKPILPDYSQSGVLVVGDVMLDRYWYGPTGRISPEAPVPVVKVENNEERPGGAANVAMNIASLGGHAHIVGLTGKDEPAE
VLKNTLGALKVKCDFVELDDYPTITKLRVMSRGQQLIRLDFEDKFENTDPELVLSRMEQALPNVRSVILSDYAKGALEHV
QSFIQKARAANVPVFIDPKGADFERYRGATLLTPNMAEFELVAGKVKSEDEMIEKGLALIEEFDFEALLVTRSEHGMTLL
RKGVEPFHLPTQAKEVYDVTGAGDTVISVLAASVAAGKPLDEACALANAAAGVVVGKLGTSTLSTIELAEAIHGSQDTDY
GVISEAALVEAVKRARAKGEKVVMTNGCFDILHAGHVSYMNHAAELGDRLIVAVNTDESVKRLKGPGRPVNPTDRRMAVL
AGLGAVDWVVPFSEDTPQRLISEVLPSILVKGGDYKPEEIAGGAEVIAAGGEVKVLNFEDGCSTTEIIKAIKGGRG

Sequences:

>Translated_476_residues
MKPILPDYSQSGVLVVGDVMLDRYWYGPTGRISPEAPVPVVKVENNEERPGGAANVAMNIASLGGHAHIVGLTGKDEPAE
VLKNTLGALKVKCDFVELDDYPTITKLRVMSRGQQLIRLDFEDKFENTDPELVLSRMEQALPNVRSVILSDYAKGALEHV
QSFIQKARAANVPVFIDPKGADFERYRGATLLTPNMAEFELVAGKVKSEDEMIEKGLALIEEFDFEALLVTRSEHGMTLL
RKGVEPFHLPTQAKEVYDVTGAGDTVISVLAASVAAGKPLDEACALANAAAGVVVGKLGTSTLSTIELAEAIHGSQDTDY
GVISEAALVEAVKRARAKGEKVVMTNGCFDILHAGHVSYMNHAAELGDRLIVAVNTDESVKRLKGPGRPVNPTDRRMAVL
AGLGAVDWVVPFSEDTPQRLISEVLPSILVKGGDYKPEEIAGGAEVIAAGGEVKVLNFEDGCSTTEIIKAIKGGRG
>Mature_476_residues
MKPILPDYSQSGVLVVGDVMLDRYWYGPTGRISPEAPVPVVKVENNEERPGGAANVAMNIASLGGHAHIVGLTGKDEPAE
VLKNTLGALKVKCDFVELDDYPTITKLRVMSRGQQLIRLDFEDKFENTDPELVLSRMEQALPNVRSVILSDYAKGALEHV
QSFIQKARAANVPVFIDPKGADFERYRGATLLTPNMAEFELVAGKVKSEDEMIEKGLALIEEFDFEALLVTRSEHGMTLL
RKGVEPFHLPTQAKEVYDVTGAGDTVISVLAASVAAGKPLDEACALANAAAGVVVGKLGTSTLSTIELAEAIHGSQDTDY
GVISEAALVEAVKRARAKGEKVVMTNGCFDILHAGHVSYMNHAAELGDRLIVAVNTDESVKRLKGPGRPVNPTDRRMAVL
AGLGAVDWVVPFSEDTPQRLISEVLPSILVKGGDYKPEEIAGGAEVIAAGGEVKVLNFEDGCSTTEIIKAIKGGRG

Specific function: Catalyzes the ADP transfer to D-glycero-D-manno-heptose 1-phosphate, yielding ADP-D,D-heptose

COG id: COG2870

COG function: function code M; ADP-heptose synthase, bifunctional sugar kinase/adenylyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the cytidylyltransferase family

Homologues:

Organism=Escherichia coli, GI1789432, Length=472, Percent_Identity=68.2203389830508, Blast_Score=656, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HLDE_VIBSL (B7VIX1)

Other databases:

- EMBL:   FM954972
- RefSeq:   YP_002416075.1
- ProteinModelPortal:   B7VIX1
- SMR:   B7VIX1
- GeneID:   7160053
- GenomeReviews:   FM954972_GR
- KEGG:   vsp:VS_0417
- HOGENOM:   HBG545535
- OMA:   LTPNRKE
- ProtClustDB:   PRK11316
- HAMAP:   MF_01603
- InterPro:   IPR023030
- InterPro:   IPR011611
- InterPro:   IPR002173
- InterPro:   IPR004821
- InterPro:   IPR004820
- InterPro:   IPR011913
- InterPro:   IPR011914
- InterPro:   IPR014729
- Gene3D:   G3DSA:3.40.50.620
- TIGRFAMs:   TIGR00125
- TIGRFAMs:   TIGR02198
- TIGRFAMs:   TIGR02199

Pfam domain/function: PF01467 CTP_transf_2; PF00294 PfkB

EC number: 2.7.-.-

Molecular weight: Translated: 50905; Mature: 50905

Theoretical pI: Translated: 4.83; Mature: 4.83

Prosite motif: PS00583 PFKB_KINASES_1; PS00584 PFKB_KINASES_2

Important sites: ACT_SITE 264-264

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPILPDYSQSGVLVVGDVMLDRYWYGPTGRISPEAPVPVVKVENNEERPGGAANVAMNI
CCCCCCCCCCCCEEEEEHHHHHHEEECCCCCCCCCCCCCEEEECCCCCCCCCHHHHHEEH
ASLGGHAHIVGLTGKDEPAEVLKNTLGALKVKCDFVELDDYPTITKLRVMSRGQQLIRLD
HHCCCCEEEEEECCCCCHHHHHHHHHHHEEEEEEEEECCCCCCHHHHHHHHCCCEEEEEC
FEDKFENTDPELVLSRMEQALPNVRSVILSDYAKGALEHVQSFIQKARAANVPVFIDPKG
CHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCC
ADFERYRGATLLTPNMAEFELVAGKVKSEDEMIEKGLALIEEFDFEALLVTRSEHGMTLL
CCHHHHCCCEEECCCCHHHHHHHCCCCCHHHHHHHHHHHHHHCCCEEEEEEECCCCHHHH
RKGVEPFHLPTQAKEVYDVTGAGDTVISVLAASVAAGKPLDEACALANAAAGVVVGKLGT
HCCCCCCCCCCCHHHHEEECCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHCEEEECCCC
STLSTIELAEAIHGSQDTDYGVISEAALVEAVKRARAKGEKVVMTNGCFDILHAGHVSYM
CHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEECCHHHHHHCCHHHHH
NHAAELGDRLIVAVNTDESVKRLKGPGRPVNPTDRRMAVLAGLGAVDWVVPFSEDTPQRL
HHHHHCCCEEEEEECCCHHHHHHCCCCCCCCCCHHHHHHHHCCCHHHEEEECCCCCHHHH
ISEVLPSILVKGGDYKPEEIAGGAEVIAAGGEVKVLNFEDGCSTTEIIKAIKGGRG
HHHHHHHHHCCCCCCCHHHHCCCCCEEECCCEEEEEEECCCCCHHHHHHHHHCCCC
>Mature Secondary Structure
MKPILPDYSQSGVLVVGDVMLDRYWYGPTGRISPEAPVPVVKVENNEERPGGAANVAMNI
CCCCCCCCCCCCEEEEEHHHHHHEEECCCCCCCCCCCCCEEEECCCCCCCCCHHHHHEEH
ASLGGHAHIVGLTGKDEPAEVLKNTLGALKVKCDFVELDDYPTITKLRVMSRGQQLIRLD
HHCCCCEEEEEECCCCCHHHHHHHHHHHEEEEEEEEECCCCCCHHHHHHHHCCCEEEEEC
FEDKFENTDPELVLSRMEQALPNVRSVILSDYAKGALEHVQSFIQKARAANVPVFIDPKG
CHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCC
ADFERYRGATLLTPNMAEFELVAGKVKSEDEMIEKGLALIEEFDFEALLVTRSEHGMTLL
CCHHHHCCCEEECCCCHHHHHHHCCCCCHHHHHHHHHHHHHHCCCEEEEEEECCCCHHHH
RKGVEPFHLPTQAKEVYDVTGAGDTVISVLAASVAAGKPLDEACALANAAAGVVVGKLGT
HCCCCCCCCCCCHHHHEEECCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHCEEEECCCC
STLSTIELAEAIHGSQDTDYGVISEAALVEAVKRARAKGEKVVMTNGCFDILHAGHVSYM
CHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEECCHHHHHHCCHHHHH
NHAAELGDRLIVAVNTDESVKRLKGPGRPVNPTDRRMAVLAGLGAVDWVVPFSEDTPQRL
HHHHHCCCEEEEEECCCHHHHHHCCCCCCCCCCHHHHHHHHCCCHHHEEEECCCCCHHHH
ISEVLPSILVKGGDYKPEEIAGGAEVIAAGGEVKVLNFEDGCSTTEIIKAIKGGRG
HHHHHHHHHCCCCCCCHHHHCCCCCEEECCCEEEEEEECCCCCHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA