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The map label for this gene is rng [H]

Identifier: 218708385

GI number: 218708385

Start: 339923

End: 341392

Strand: Direct

Name: rng [H]

Synonym: VS_0345

Alternate gene names: 218708385

Gene position: 339923-341392 (Clockwise)

Preceding gene: 218708384

Following gene: 218708386

Centisome position: 10.3

GC content: 45.51

Gene sequence:

>1470_bases
ATGAGTGCTGAATTGTTGCTGAACGTGACCCCGAGTGAGACTCGTGTGGCCATGATTGAAGGGGGAGCTCTTCAAGAGAT
CCATGTCGAACGAGATGCTCGACGCGGGATCGTAGGAAATATCTACAAAGGACGTGTAAGCCGTGTTTTGCCTGGAATGC
AGGCGGCATTTGTGGATATTGGCCTTGATAAAGCAGCTTTTTTGCACGCCTCTGATATTGTCCCCCACACTGAATGTGTT
GCTGAGAATGAAAAGAAGCAATTTCAGGTTCGTGATATTTCAGAGCTTGTTCGCCAAGGGCAAGATATTGTCGTTCAAGT
GGTCAAAGATCCTCTTGGTACCAAAGGTGCCCGTCTAACCACTGACATTACATTACCATCTCGTTATCTGGTATTCATGC
CGGGCGCAAGCCACGTTGGTGTTTCTCAGAGAATTGATAGTGAGTCAGAACGCAACCGCCTAAAAAAAGTCGTGTCTCGT
TACTGTGACGAGCACGGTGGCTTCATCATCCGTACGGCAGCAGAAGGCGCAGATTCAAATGAGTTGGCGCAAGATGCCGC
ATTCTTGAAGCGATTATGGCTAAAGGTGTTAGAACGTCGTGGTAAGCACAAAGCTCGTACTCGCTTGTATGGTGAGTTGT
GCTTAAGCCAACGCATCTTACGTGACTTTGTCGGTACTGAGCTGAGCAAGATTCAGGTCGATTCTCGTCTAGAATATGAA
AACCTAAAAGAGTTTACTTCTGAATACGTACCTGAGCTAACAGACAAGCTTGAGTTGTATGAAGGTGATAAGCCTATCTT
TGATATGTACGATACCGAGAACGAAATTCAACGTTCATTGGATCGCAAAGTCGAATTGAAGTCTGGTGGGTATCTGATTA
TCGACCAAACAGAAGCGATGACCACTGTTGATATCAATACTGGGGCCTTTGTTGGTCGTCGTAATCTAGAAGAGACGATT
TTCAATACTAACGTCGAAGCGACACAAGCTATTGCTCGTCAACTTCGCCTTCGTAATTTAGGCGGCATCATCATTATCGA
TTTTATTGATATGCTGTCTGAAGAACACCGTAAGCGAGTACTAACTTCTTTAGAAGCTGCATTAGACAAAGACCGTGTGA
AAACCAACATTAATGGCTTCACGCAGCTTGGTTTGGTTGAGATGACTCGTAAACGTACTCGTGAAAGTATTGAGCATATT
CTGTGTTCTAGCTGCCCTGCTTGTGAAGGTCGCGGTAGCGTGAAGACAGTTGAAACCGTTTGTTATGAAATTCTTCGAGA
GATCACACGAGTGAATCGCGCGTATGACGCCGATAAGTTTGTTGTTTATGCGGCGGCTGCCGTTGCCGAGGCGTTAGAAG
GCGAGGAATCTCACGCGCTTGCTGAGCTTGAAGTGTTTATTGGTAAGCAAGTTAAAATCCAGGCTGAGCCTCTGTACATA
CAAGAGCAGTTTGATGTTGTTATGATGTAA

Upstream 100 bases:

>100_bases
CCCGAATCGAAGGTAGTTATTACGCCGTTGTCGGCTTACCTTTATTTGAAACGGACCAGCTACTGCAAGAATTCTTATAA
TTACTAATCTGAGGTGCACC

Downstream 100 bases:

>100_bases
TGGATATTTTGTGAGTTCAAGCGTTACTCTGATTCTTCGTACATGTTTATGGTTAGTGGTTACTCTCTTAGTAACACTAG
CCATTGCTGTCACTACACTG

Product: ribonuclease G

Products: NA

Alternate protein names: RNase G; Cytoplasmic axial filament protein [H]

Number of amino acids: Translated: 489; Mature: 488

Protein sequence:

>489_residues
MSAELLLNVTPSETRVAMIEGGALQEIHVERDARRGIVGNIYKGRVSRVLPGMQAAFVDIGLDKAAFLHASDIVPHTECV
AENEKKQFQVRDISELVRQGQDIVVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIDSESERNRLKKVVSR
YCDEHGGFIIRTAAEGADSNELAQDAAFLKRLWLKVLERRGKHKARTRLYGELCLSQRILRDFVGTELSKIQVDSRLEYE
NLKEFTSEYVPELTDKLELYEGDKPIFDMYDTENEIQRSLDRKVELKSGGYLIIDQTEAMTTVDINTGAFVGRRNLEETI
FNTNVEATQAIARQLRLRNLGGIIIIDFIDMLSEEHRKRVLTSLEAALDKDRVKTNINGFTQLGLVEMTRKRTRESIEHI
LCSSCPACEGRGSVKTVETVCYEILREITRVNRAYDADKFVVYAAAAVAEALEGEESHALAELEVFIGKQVKIQAEPLYI
QEQFDVVMM

Sequences:

>Translated_489_residues
MSAELLLNVTPSETRVAMIEGGALQEIHVERDARRGIVGNIYKGRVSRVLPGMQAAFVDIGLDKAAFLHASDIVPHTECV
AENEKKQFQVRDISELVRQGQDIVVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIDSESERNRLKKVVSR
YCDEHGGFIIRTAAEGADSNELAQDAAFLKRLWLKVLERRGKHKARTRLYGELCLSQRILRDFVGTELSKIQVDSRLEYE
NLKEFTSEYVPELTDKLELYEGDKPIFDMYDTENEIQRSLDRKVELKSGGYLIIDQTEAMTTVDINTGAFVGRRNLEETI
FNTNVEATQAIARQLRLRNLGGIIIIDFIDMLSEEHRKRVLTSLEAALDKDRVKTNINGFTQLGLVEMTRKRTRESIEHI
LCSSCPACEGRGSVKTVETVCYEILREITRVNRAYDADKFVVYAAAAVAEALEGEESHALAELEVFIGKQVKIQAEPLYI
QEQFDVVMM
>Mature_488_residues
SAELLLNVTPSETRVAMIEGGALQEIHVERDARRGIVGNIYKGRVSRVLPGMQAAFVDIGLDKAAFLHASDIVPHTECVA
ENEKKQFQVRDISELVRQGQDIVVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIDSESERNRLKKVVSRY
CDEHGGFIIRTAAEGADSNELAQDAAFLKRLWLKVLERRGKHKARTRLYGELCLSQRILRDFVGTELSKIQVDSRLEYEN
LKEFTSEYVPELTDKLELYEGDKPIFDMYDTENEIQRSLDRKVELKSGGYLIIDQTEAMTTVDINTGAFVGRRNLEETIF
NTNVEATQAIARQLRLRNLGGIIIIDFIDMLSEEHRKRVLTSLEAALDKDRVKTNINGFTQLGLVEMTRKRTRESIEHIL
CSSCPACEGRGSVKTVETVCYEILREITRVNRAYDADKFVVYAAAAVAEALEGEESHALAELEVFIGKQVKIQAEPLYIQ
EQFDVVMM

Specific function: Involved in the processing of the 5'-end of 16S rRNA. Could be involved in chromosome segregation and cell division. It may be one of the components of the cytoplasmic axial filaments bundles or merely regulate the formation of this structure [H]

COG id: COG1530

COG function: function code J; Ribonucleases G and E

Gene ontology:

Cell location: Cytoplasm, cytoskeleton [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Escherichia coli, GI87082235, Length=489, Percent_Identity=79.959100204499, Blast_Score=799, Evalue=0.0,
Organism=Escherichia coli, GI1787325, Length=432, Percent_Identity=35.4166666666667, Blast_Score=262, Evalue=4e-71,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR003029
- InterPro:   IPR019307
- InterPro:   IPR022967
- InterPro:   IPR004659 [H]

Pfam domain/function: PF10150 RNase_E_G; PF00575 S1 [H]

EC number: 3.1.4.- [C]

Molecular weight: Translated: 55131; Mature: 55000

Theoretical pI: Translated: 5.47; Mature: 5.47

Prosite motif: PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAELLLNVTPSETRVAMIEGGALQEIHVERDARRGIVGNIYKGRVSRVLPGMQAAFVDI
CCCCEEEEECCCCCEEEEEECCCEEHHHHHHHHCCCCCCCHHHHHHHHHCCCHHHHHHCC
GLDKAAFLHASDIVPHTECVAENEKKQFQVRDISELVRQGQDIVVQVVKDPLGTKGARLT
CCCHHHHEEHHCCCCCHHHHHCCCHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCEEE
TDITLPSRYLVFMPGASHVGVSQRIDSESERNRLKKVVSRYCDEHGGFIIRTAAEGADSN
EEEECCCCEEEEECCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCH
ELAQDAAFLKRLWLKVLERRGKHKARTRLYGELCLSQRILRDFVGTELSKIQVDSRLEYE
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHEEECCCCCHH
NLKEFTSEYVPELTDKLELYEGDKPIFDMYDTENEIQRSLDRKVELKSGGYLIIDQTEAM
HHHHHHHHHHHHHHHHHHHHCCCCCCEECCCCHHHHHHHHHCEEEECCCCEEEEECCCCE
TTVDINTGAFVGRRNLEETIFNTNVEATQAIARQLRLRNLGGIIIIDFIDMLSEEHRKRV
EEEECCCCCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHH
LTSLEAALDKDRVKTNINGFTQLGLVEMTRKRTRESIEHILCSSCPACEGRGSVKTVETV
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHH
CYEILREITRVNRAYDADKFVVYAAAAVAEALEGEESHALAELEVFIGKQVKIQAEPLYI
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCEEEEEECCEEE
QEQFDVVMM
ECCCCEEEC
>Mature Secondary Structure 
SAELLLNVTPSETRVAMIEGGALQEIHVERDARRGIVGNIYKGRVSRVLPGMQAAFVDI
CCCEEEEECCCCCEEEEEECCCEEHHHHHHHHCCCCCCCHHHHHHHHHCCCHHHHHHCC
GLDKAAFLHASDIVPHTECVAENEKKQFQVRDISELVRQGQDIVVQVVKDPLGTKGARLT
CCCHHHHEEHHCCCCCHHHHHCCCHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCEEE
TDITLPSRYLVFMPGASHVGVSQRIDSESERNRLKKVVSRYCDEHGGFIIRTAAEGADSN
EEEECCCCEEEEECCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCH
ELAQDAAFLKRLWLKVLERRGKHKARTRLYGELCLSQRILRDFVGTELSKIQVDSRLEYE
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHEEECCCCCHH
NLKEFTSEYVPELTDKLELYEGDKPIFDMYDTENEIQRSLDRKVELKSGGYLIIDQTEAM
HHHHHHHHHHHHHHHHHHHHCCCCCCEECCCCHHHHHHHHHCEEEECCCCEEEEECCCCE
TTVDINTGAFVGRRNLEETIFNTNVEATQAIARQLRLRNLGGIIIIDFIDMLSEEHRKRV
EEEECCCCCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHH
LTSLEAALDKDRVKTNINGFTQLGLVEMTRKRTRESIEHILCSSCPACEGRGSVKTVETV
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHH
CYEILREITRVNRAYDADKFVVYAAAAVAEALEGEESHALAELEVFIGKQVKIQAEPLYI
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCEEEEEECCEEE
QEQFDVVMM
ECCCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]