Click here to switch to the map view.

The map label for this gene is outD [H]

Identifier: 218708368

GI number: 218708368

Start: 321625

End: 323289

Strand: Direct

Name: outD [H]

Synonym: VS_0328

Alternate gene names: 218708368

Gene position: 321625-323289 (Clockwise)

Preceding gene: 218708367

Following gene: 218708369

Centisome position: 9.75

GC content: 44.62

Gene sequence:

>1665_bases
ATGCGTAAACTTGTAGTAGCAATTCTAGTGTCATCTTTAGTCGGCTGTTCGATGGGGCATAGAGACCCTGTTGAGATAAA
AGAGTCTTTAAACGAATCTATTAATGAAGCGAATAGCAAAGCACTTCATAACTTACCTTCGTCGGTACAAGATGACCTTA
TGCCTCAGCTAAGTTCGGACTCTATGTCTCCGAGTGTGGAAACCGTCAAGCGCTTTCGTATTCAAGCAAAAGGCGTTGAA
GCGAGAACCTTTTTTGCAAGCCTAGTGAAAGGTACAGAGTACAGTGCCGCGATTCATCCAAGCGTTGCTGGAAAACTCAC
TCTAAATCTAACAGACGTGACGTTAGATGAAGTACTGGCTGTTGCTCAGGATATGTATGGTTACGATATTGAAAAGCGTG
GCAAGGTGATTCAAATTTATCCAGCTGGTTTGCGTACAGTCACGATTCCTGTCGATTACTTGCAAGTAAAGCGCTCAGGT
CGTTCTTTGACAACGATAACCACAGGAACAATTACAAATTCAGACTCTAAGCCATCGGGTTCTTCTAATTCTAGTTCTAA
GTCTGATTCGTCCAATTCATCTGATTCGTCGAGCTCTACATCGAGCGGCGGTACAGAGATCGAAACGATCTCTGAAAGTG
ATTTTTGGCCGCAGCTTGAAGTAGCTGTCGCCCACTTAATTGGTTCTGGTGATGGGCAAAGTGTAGTAGTTACGCCGCAA
GCAAGTGTGATTACGGTGCGAGCTTACCCGGATGAAATTCGTGAGGTTCGTGAGTTCCTTGGTATTTCTCAGAAACGCCT
GCAGCGCCAAGTTATCTTGGAAGCAAAGATCATGGAAGTGACGTTGAGTGATGGCTATCAGCAGGGTATTAACTGGACCA
AGATGTTTTCGTCGAACGGAACTAACTACACGGTCGGTATGGGCTCAATTGTTAAAAATGCGGCTGGTACGATCATCCCT
GGTACATTACCAGGCATGGATCCAATCGGTGCGGCTTTAGGTGGTCAATCTAATCTTGTTGTCTCTGGAGGTAGTTTTGA
AGCCGTCTTGAGTTTCATGGACACACAGGGTGACTTGAATGTGTTATCTAGCCCAAGAGTGACAGCAGCTAATAACCAGA
AAGCGGTTATTAAAGTGGGCACTGACGAATACTATGTGACTGAATTATCAAGTTCAGTGGGGAGTGGGGATAACGCGAAT
GTGGCCCCTGAAGTTGAGTTAACCCCATTCTTCTCTGGTATTTCGTTGGATGTGACGCCGCAGATCGATGATAAAGGTAA
TGTGTTTTTACATGTTCACCCTGCCGTTATTGAGGTGGAAGAGGAAGTGAAAGAGCTCAACCTAGGATCAACAACAGGCA
TTGTGAAGCTGCCTTTGGCAAAAAGTTCGATTCGTGAGTCGGACTCAGTGATTCGAGCACAGGATGGTGATGTTGTTGTT
ATTGGTGGATTGATGAAATCAAATACCAGTGACATGACCTCAAAAGTTCCATTTCTTGGTGATATTCCAGCGTTAGGTCA
TTTATTCCGCAATACCAGTCAGTTGACTCAAAAAACAGAGTTAGTGATTCTATTGAAACCAACGATTGTGGGTGTGAACA
CTTGGCTAACCGAGTTGGAGCGTTCTCGTGACCTTCTTCAGCAATGGTTCCCTGATGAAGAATAA

Upstream 100 bases:

>100_bases
CATTGATCCAGAGTACGTCACTCTGCAGAGAAGCTCTAAGCAGTGGAAATTAGAGATGTTCTCTTTAGATGTTAAGAATA
ATTAAGGTTAGAGTGAAGAC

Downstream 100 bases:

>100_bases
TTGCTCATCGTCTCAATGTAGATTGGTCGTCATTGACTAAGCTGGATCATCTTATGTATCAAGCTCACTTTAGTTTTGAA
CAGTTACCGTTTACCTTAAC

Product: MSHA biogenesis protein MshL

Products: NA

Alternate protein names: Pectic enzymes secretion protein outD [H]

Number of amino acids: Translated: 554; Mature: 554

Protein sequence:

>554_residues
MRKLVVAILVSSLVGCSMGHRDPVEIKESLNESINEANSKALHNLPSSVQDDLMPQLSSDSMSPSVETVKRFRIQAKGVE
ARTFFASLVKGTEYSAAIHPSVAGKLTLNLTDVTLDEVLAVAQDMYGYDIEKRGKVIQIYPAGLRTVTIPVDYLQVKRSG
RSLTTITTGTITNSDSKPSGSSNSSSKSDSSNSSDSSSSTSSGGTEIETISESDFWPQLEVAVAHLIGSGDGQSVVVTPQ
ASVITVRAYPDEIREVREFLGISQKRLQRQVILEAKIMEVTLSDGYQQGINWTKMFSSNGTNYTVGMGSIVKNAAGTIIP
GTLPGMDPIGAALGGQSNLVVSGGSFEAVLSFMDTQGDLNVLSSPRVTAANNQKAVIKVGTDEYYVTELSSSVGSGDNAN
VAPEVELTPFFSGISLDVTPQIDDKGNVFLHVHPAVIEVEEEVKELNLGSTTGIVKLPLAKSSIRESDSVIRAQDGDVVV
IGGLMKSNTSDMTSKVPFLGDIPALGHLFRNTSQLTQKTELVILLKPTIVGVNTWLTELERSRDLLQQWFPDEE

Sequences:

>Translated_554_residues
MRKLVVAILVSSLVGCSMGHRDPVEIKESLNESINEANSKALHNLPSSVQDDLMPQLSSDSMSPSVETVKRFRIQAKGVE
ARTFFASLVKGTEYSAAIHPSVAGKLTLNLTDVTLDEVLAVAQDMYGYDIEKRGKVIQIYPAGLRTVTIPVDYLQVKRSG
RSLTTITTGTITNSDSKPSGSSNSSSKSDSSNSSDSSSSTSSGGTEIETISESDFWPQLEVAVAHLIGSGDGQSVVVTPQ
ASVITVRAYPDEIREVREFLGISQKRLQRQVILEAKIMEVTLSDGYQQGINWTKMFSSNGTNYTVGMGSIVKNAAGTIIP
GTLPGMDPIGAALGGQSNLVVSGGSFEAVLSFMDTQGDLNVLSSPRVTAANNQKAVIKVGTDEYYVTELSSSVGSGDNAN
VAPEVELTPFFSGISLDVTPQIDDKGNVFLHVHPAVIEVEEEVKELNLGSTTGIVKLPLAKSSIRESDSVIRAQDGDVVV
IGGLMKSNTSDMTSKVPFLGDIPALGHLFRNTSQLTQKTELVILLKPTIVGVNTWLTELERSRDLLQQWFPDEE
>Mature_554_residues
MRKLVVAILVSSLVGCSMGHRDPVEIKESLNESINEANSKALHNLPSSVQDDLMPQLSSDSMSPSVETVKRFRIQAKGVE
ARTFFASLVKGTEYSAAIHPSVAGKLTLNLTDVTLDEVLAVAQDMYGYDIEKRGKVIQIYPAGLRTVTIPVDYLQVKRSG
RSLTTITTGTITNSDSKPSGSSNSSSKSDSSNSSDSSSSTSSGGTEIETISESDFWPQLEVAVAHLIGSGDGQSVVVTPQ
ASVITVRAYPDEIREVREFLGISQKRLQRQVILEAKIMEVTLSDGYQQGINWTKMFSSNGTNYTVGMGSIVKNAAGTIIP
GTLPGMDPIGAALGGQSNLVVSGGSFEAVLSFMDTQGDLNVLSSPRVTAANNQKAVIKVGTDEYYVTELSSSVGSGDNAN
VAPEVELTPFFSGISLDVTPQIDDKGNVFLHVHPAVIEVEEEVKELNLGSTTGIVKLPLAKSSIRESDSVIRAQDGDVVV
IGGLMKSNTSDMTSKVPFLGDIPALGHLFRNTSQLTQKTELVILLKPTIVGVNTWLTELERSRDLLQQWFPDEE

Specific function: Involved in a general secretion pathway (GSP) for the export of proteins. Required for the translocation of the multiple pectic enzymes [H]

COG id: COG1450

COG function: function code NU; Type II secretory pathway, component PulD

Gene ontology:

Cell location: Cell outer membrane (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP D family [H]

Homologues:

Organism=Escherichia coli, GI87082242, Length=290, Percent_Identity=30, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI1789793, Length=263, Percent_Identity=26.2357414448669, Blast_Score=72, Evalue=7e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001775
- InterPro:   IPR005644
- InterPro:   IPR004846
- InterPro:   IPR013356
- InterPro:   IPR004845 [H]

Pfam domain/function: PF00263 Secretin; PF03958 Secretin_N [H]

EC number: NA

Molecular weight: Translated: 59334; Mature: 59334

Theoretical pI: Translated: 4.66; Mature: 4.66

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKLVVAILVSSLVGCSMGHRDPVEIKESLNESINEANSKALHNLPSSVQDDLMPQLSSD
CHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCC
SMSPSVETVKRFRIQAKGVEARTFFASLVKGTEYSAAIHPSVAGKLTLNLTDVTLDEVLA
CCCCHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCEEECCCCCCEEEEEEECCCHHHHHH
VAQDMYGYDIEKRGKVIQIYPAGLRTVTIPVDYLQVKRSGRSLTTITTGTITNSDSKPSG
HHHHHHCCCHHHCCCEEEEEECCCEEEEEEHHHHHHHCCCCEEEEEEEEEEECCCCCCCC
SSNSSSKSDSSNSSDSSSSTSSGGTEIETISESDFWPQLEVAVAHLIGSGDGQSVVVTPQ
CCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCHHHHHHHHHHCCCCCCEEEECCC
ASVITVRAYPDEIREVREFLGISQKRLQRQVILEAKIMEVTLSDGYQQGINWTKMFSSNG
CCEEEEEECCHHHHHHHHHHCCCHHHHHHHHHHHEEEEEEEECCCHHHCCCEEEEECCCC
TNYTVGMGSIVKNAAGTIIPGTLPGMDPIGAALGGQSNLVVSGGSFEAVLSFMDTQGDLN
CEEEEEHHHHHHHCCCCCCCCCCCCCCCHHHHHCCCCCEEEECCCHHHHHHHHCCCCCCE
VLSSPRVTAANNQKAVIKVGTDEYYVTELSSSVGSGDNANVAPEVELTPFFSGISLDVTP
ECCCCCEEECCCCEEEEEECCCCEEEEEHHHHCCCCCCCCCCCCEEECEEECCCEEEECC
QIDDKGNVFLHVHPAVIEVEEEVKELNLGSTTGIVKLPLAKSSIRESDSVIRAQDGDVVV
CCCCCCCEEEEECCCEEEEHHHHHHCCCCCCCCEEEECCCHHHHHCCCCEEEECCCCEEE
IGGLMKSNTSDMTSKVPFLGDIPALGHLFRNTSQLTQKTELVILLKPTIVGVNTWLTELE
ECCEECCCCCHHHHCCCCCCCCHHHHHHHHCHHHHHCCCCEEEEEECCEEEHHHHHHHHH
RSRDLLQQWFPDEE
HHHHHHHHHCCCCC
>Mature Secondary Structure
MRKLVVAILVSSLVGCSMGHRDPVEIKESLNESINEANSKALHNLPSSVQDDLMPQLSSD
CHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCC
SMSPSVETVKRFRIQAKGVEARTFFASLVKGTEYSAAIHPSVAGKLTLNLTDVTLDEVLA
CCCCHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCEEECCCCCCEEEEEEECCCHHHHHH
VAQDMYGYDIEKRGKVIQIYPAGLRTVTIPVDYLQVKRSGRSLTTITTGTITNSDSKPSG
HHHHHHCCCHHHCCCEEEEEECCCEEEEEEHHHHHHHCCCCEEEEEEEEEEECCCCCCCC
SSNSSSKSDSSNSSDSSSSTSSGGTEIETISESDFWPQLEVAVAHLIGSGDGQSVVVTPQ
CCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCHHHHHHHHHHCCCCCCEEEECCC
ASVITVRAYPDEIREVREFLGISQKRLQRQVILEAKIMEVTLSDGYQQGINWTKMFSSNG
CCEEEEEECCHHHHHHHHHHCCCHHHHHHHHHHHEEEEEEEECCCHHHCCCEEEEECCCC
TNYTVGMGSIVKNAAGTIIPGTLPGMDPIGAALGGQSNLVVSGGSFEAVLSFMDTQGDLN
CEEEEEHHHHHHHCCCCCCCCCCCCCCCHHHHHCCCCCEEEECCCHHHHHHHHCCCCCCE
VLSSPRVTAANNQKAVIKVGTDEYYVTELSSSVGSGDNANVAPEVELTPFFSGISLDVTP
ECCCCCEEECCCCEEEEEECCCCEEEEEHHHHCCCCCCCCCCCCEEECEEECCCEEEECC
QIDDKGNVFLHVHPAVIEVEEEVKELNLGSTTGIVKLPLAKSSIRESDSVIRAQDGDVVV
CCCCCCCEEEEECCCEEEEHHHHHHCCCCCCCCEEEECCCHHHHHCCCCEEEECCCCEEE
IGGLMKSNTSDMTSKVPFLGDIPALGHLFRNTSQLTQKTELVILLKPTIVGVNTWLTELE
ECCEECCCCCHHHHCCCCCCCCHHHHHHHHCHHHHHCCCCEEEEEECCEEEHHHHHHHHH
RSRDLLQQWFPDEE
HHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1429461 [H]