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The map label for this gene is dusA [H]

Identifier: 218708347

GI number: 218708347

Start: 287329

End: 288330

Strand: Direct

Name: dusA [H]

Synonym: VS_0294

Alternate gene names: 218708347

Gene position: 287329-288330 (Clockwise)

Preceding gene: 218708346

Following gene: 218708349

Centisome position: 8.71

GC content: 47.9

Gene sequence:

>1002_bases
ATGAAACCTGACAATACCAGTAAATACGCGGCTAACCGCTTATCCGTTGCACCCATGTTGGATTGGACTGACCGCCACTG
TCGTTACTTTCACCGTTTGCTATCTCAGCAGACGCTTCTGTACACGGAAATGGTCACAACAGGCGCGATCTTACATGGTA
AGGGCGACTTTCTAGAATATAACGAGCAAGAACATCCATTAGCTCTTCAACTTGGTGGTTCAAACCCGGTTGATTTGGCG
GCTTGTGCCAAGCTTGCTGGAGAGCGTGGCTATGATGAAGTAAACCTAAACGTAGGTTGCCCTTCAGACCGAGTTCAGAA
TGGGCGCTTTGGTGCTTGCCTAATGGCTGAGCCTGAGCTGGTGGCAGATTGTGTGTCAGCGATGAAAGAAGTCACGGATA
TTCCAATTACGGTGAAAACGCGTATTGGTATCGACGACCAAGATTCTTACGAGTTTCTGACTAAGTTTGTTTCTACTGTT
TCTGAAAAGGGCGGTTGTGAGCAATTTACTATCCATGCGCGTAAGGCGTGGTTGAGTGGCCTTAGCCCGAAAGAGAACCG
TGAGATCCCACCGCTAGATTACGATCGCGCGTACCAAATCAAGAAAGACTTCTCAGATCTTGTGATTGCGGTAAATGGTG
GCATCACTACATTAGAACAAACTAAAGAGCACTTGCAGCACCTTGATGGTGTGATGATCGGTCGTGAGGCATACCATAGC
CCGTTTATCTTGGCTGAAGTTGATCAGCAGATCTTCGGTTTAGACACGCCAATCAAGAAGCGTTCACAAGTGGTTGAAGA
GATGTACCCGTACATTGAGCGTCAGCTTTCGAATGGAGCGAGCCTTGGTCATATCTCTCGTCACATGCTTGGTTTGTTCC
AAAGCATGCCGGGCGCAAGACAATGGCGTCGTTACATCAGTGAGAATGCACATAAGAAAGGCGCGGGCATTGAGGTGATT
CAGACGGCATTGGCTAAGATCCCTAAAGAGCTAAACGTATAG

Upstream 100 bases:

>100_bases
CCATCAATACGTGATTTAGGCGTAGTGAGTGGATGAGTTTGCGGCAGAACTAGCGAGTTTCTGTATATATATGCAGTGTA
TAGATTTTGAGTAACGCTTT

Downstream 100 bases:

>100_bases
ACACCCTCAAGATTAAAGCAAAAGCCAAAAGCTAAGAATTAAAAGTTAGAAGCTTAAGGTTTAAATAACAAGGCTAAATT
CGCCATTAGAGGCTAAATTT

Product: tRNA-dihydrouridine synthase A

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 333; Mature: 333

Protein sequence:

>333_residues
MKPDNTSKYAANRLSVAPMLDWTDRHCRYFHRLLSQQTLLYTEMVTTGAILHGKGDFLEYNEQEHPLALQLGGSNPVDLA
ACAKLAGERGYDEVNLNVGCPSDRVQNGRFGACLMAEPELVADCVSAMKEVTDIPITVKTRIGIDDQDSYEFLTKFVSTV
SEKGGCEQFTIHARKAWLSGLSPKENREIPPLDYDRAYQIKKDFSDLVIAVNGGITTLEQTKEHLQHLDGVMIGREAYHS
PFILAEVDQQIFGLDTPIKKRSQVVEEMYPYIERQLSNGASLGHISRHMLGLFQSMPGARQWRRYISENAHKKGAGIEVI
QTALAKIPKELNV

Sequences:

>Translated_333_residues
MKPDNTSKYAANRLSVAPMLDWTDRHCRYFHRLLSQQTLLYTEMVTTGAILHGKGDFLEYNEQEHPLALQLGGSNPVDLA
ACAKLAGERGYDEVNLNVGCPSDRVQNGRFGACLMAEPELVADCVSAMKEVTDIPITVKTRIGIDDQDSYEFLTKFVSTV
SEKGGCEQFTIHARKAWLSGLSPKENREIPPLDYDRAYQIKKDFSDLVIAVNGGITTLEQTKEHLQHLDGVMIGREAYHS
PFILAEVDQQIFGLDTPIKKRSQVVEEMYPYIERQLSNGASLGHISRHMLGLFQSMPGARQWRRYISENAHKKGAGIEVI
QTALAKIPKELNV
>Mature_333_residues
MKPDNTSKYAANRLSVAPMLDWTDRHCRYFHRLLSQQTLLYTEMVTTGAILHGKGDFLEYNEQEHPLALQLGGSNPVDLA
ACAKLAGERGYDEVNLNVGCPSDRVQNGRFGACLMAEPELVADCVSAMKEVTDIPITVKTRIGIDDQDSYEFLTKFVSTV
SEKGGCEQFTIHARKAWLSGLSPKENREIPPLDYDRAYQIKKDFSDLVIAVNGGITTLEQTKEHLQHLDGVMIGREAYHS
PFILAEVDQQIFGLDTPIKKRSQVVEEMYPYIERQLSNGASLGHISRHMLGLFQSMPGARQWRRYISENAHKKGAGIEVI
QTALAKIPKELNV

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family. DusA subfamily [H]

Homologues:

Organism=Homo sapiens, GI31742496, Length=296, Percent_Identity=24.6621621621622, Blast_Score=75, Evalue=7e-14,
Organism=Homo sapiens, GI239788483, Length=213, Percent_Identity=26.7605633802817, Blast_Score=73, Evalue=4e-13,
Organism=Homo sapiens, GI239788462, Length=213, Percent_Identity=26.7605633802817, Blast_Score=70, Evalue=2e-12,
Organism=Escherichia coli, GI145693211, Length=322, Percent_Identity=71.1180124223602, Blast_Score=496, Evalue=1e-141,
Organism=Escherichia coli, GI1789660, Length=241, Percent_Identity=27.8008298755187, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1788462, Length=243, Percent_Identity=26.3374485596708, Blast_Score=70, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17507177, Length=170, Percent_Identity=30.5882352941176, Blast_Score=72, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI25144369, Length=193, Percent_Identity=29.0155440414508, Blast_Score=72, Evalue=4e-13,
Organism=Saccharomyces cerevisiae, GI6323560, Length=269, Percent_Identity=26.3940520446097, Blast_Score=74, Evalue=4e-14,
Organism=Drosophila melanogaster, GI24580595, Length=314, Percent_Identity=23.2484076433121, Blast_Score=75, Evalue=8e-14,
Organism=Drosophila melanogaster, GI19920448, Length=314, Percent_Identity=23.2484076433121, Blast_Score=75, Evalue=8e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004653
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: 1.-.-.-

Molecular weight: Translated: 37295; Mature: 37295

Theoretical pI: Translated: 6.44; Mature: 6.44

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPDNTSKYAANRLSVAPMLDWTDRHCRYFHRLLSQQTLLYTEMVTTGAILHGKGDFLEY
CCCCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCEEEECCCCCCCC
NEQEHPLALQLGGSNPVDLAACAKLAGERGYDEVNLNVGCPSDRVQNGRFGACLMAEPEL
CCCCCCEEEEECCCCCHHHHHHHHHHCCCCCCEEEEECCCCHHHHCCCCCCEEEECCHHH
VADCVSAMKEVTDIPITVKTRIGIDDQDSYEFLTKFVSTVSEKGGCEQFTIHARKAWLSG
HHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCC
LSPKENREIPPLDYDRAYQIKKDFSDLVIAVNGGITTLEQTKEHLQHLDGVMIGREAYHS
CCCCCCCCCCCCCCCHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHHHCCHHHHCC
PFILAEVDQQIFGLDTPIKKRSQVVEEMYPYIERQLSNGASLGHISRHMLGLFQSMPGAR
CEEEEHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCHH
QWRRYISENAHKKGAGIEVIQTALAKIPKELNV
HHHHHHHHCCCCCCCCHHHHHHHHHHCHHHCCC
>Mature Secondary Structure
MKPDNTSKYAANRLSVAPMLDWTDRHCRYFHRLLSQQTLLYTEMVTTGAILHGKGDFLEY
CCCCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCEEEECCCCCCCC
NEQEHPLALQLGGSNPVDLAACAKLAGERGYDEVNLNVGCPSDRVQNGRFGACLMAEPEL
CCCCCCEEEEECCCCCHHHHHHHHHHCCCCCCEEEEECCCCHHHHCCCCCCEEEECCHHH
VADCVSAMKEVTDIPITVKTRIGIDDQDSYEFLTKFVSTVSEKGGCEQFTIHARKAWLSG
HHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCC
LSPKENREIPPLDYDRAYQIKKDFSDLVIAVNGGITTLEQTKEHLQHLDGVMIGREAYHS
CCCCCCCCCCCCCCCHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHHHCCHHHHCC
PFILAEVDQQIFGLDTPIKKRSQVVEEMYPYIERQLSNGASLGHISRHMLGLFQSMPGAR
CEEEEHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCHH
QWRRYISENAHKKGAGIEVIQTALAKIPKELNV
HHHHHHHHCCCCCCCCHHHHHHHHHHCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12620739 [H]