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The map label for this gene is dnaB [H]

Identifier: 218708337

GI number: 218708337

Start: 277788

End: 279179

Strand: Direct

Name: dnaB [H]

Synonym: VS_0284

Alternate gene names: 218708337

Gene position: 277788-279179 (Clockwise)

Preceding gene: 218708335

Following gene: 218708338

Centisome position: 8.42

GC content: 45.76

Gene sequence:

>1392_bases
GTGGATACCAAAAGTCAGAAATCAGCCAACGATCAGGTGGACGCCATCAAGGTCCCGCCACATTCATTAGAAGCTGAGCA
ATCTGTTATTGGCGGTTTGTTATTGGATAACGAACGCTGGGATACGGTGGCCGAAAAGGTTGTGGCCAAAGACTTTTATA
GCCGTCCTCACCGTCTGATCTTTGAAGCGGTAAAGGATATCCTTGAAGAAAGCTCTCCTCTGGATCTTATTACACTCTCT
GAACATTTAGAGCTGCGTGAGCAACTTGAAGAAGTGGGTGGCTTTGCTTACCTTGCTGACCTAGCGAAAAATACACCAAG
TGCAGCAAACATCAATGCCTATGCAGATATCGTGGCACAACGTGCATTGGTGCGTAGCCTGATTGGTGTGGCAAATGAAA
TTGCGGATTCTGGTTATGACCCTCAAGGTCGTACATCGGAAGAACTTGTTGATCTTGCTGAGAGTAAAGTTTTCGCGATT
GCCGAAGGCCGAGCAAGTGAAAATGAAGGTCCACAAAACGTTGATAGCATTCTAGAGAAGACGCTAGAACGTATCGAAAT
CTTATATAAATCGCCACAAGATGGTGTGACAGGTGTCGATACGGGTTTCAACGACCTCAATAAGAAAACGGCAGGCCTTC
AGGGTTCTGACTTAATCATTGTTGCTGCGCGTCCATCGATGGGTAAAACCACGTTTGCGATGAACCTATGTGAAAACGCA
GCGATGAAGCAAGACAAGCCAGTGTTAATCTTCTCGCTAGAGATGCCAGCTGAACAGCTGATGATGCGTATGCTTGCATC
ACTTTCTCGCGTAGACCAAACCAAGATTCGTACCGGTCAGTTAGACGATGAAGATTGGGCTCGTATCTCGTCGAGTATGG
GTATTCTGATGGATAAGAAGAATATGTATATCGATGACAGCTCGGGTCTAACGCCAACAGAAGTACGTTCTCGAGCTCGA
CGAATTGCTCGTGAGCATGATGGTATCTCTATGATCATGATAGATTACCTTCAATTAATGCGTGTACCTTCGTTATCTGA
TAACCGTACTCTAGAGATCGCCGAGATTTCTCGCTCATTGAAAGCACTAGCAAAAGAGCTCAACGTACCAGTTGTGGCAC
TTTCTCAGCTTAACCGTTCCCTAGAGCAACGTGCTGACAAGCGCCCAGTAAACTCGGATTTGCGTGAATCAGGTTCGATC
GAGCAAGATGCCGACTTAATCATGTTTATCTATCGTGATGAGGTTTATAACCCAGATAGTTCATTGAAAGGCATCGCTGA
AATCATCCTTGGTAAGCAACGTAACGGTCCGATCGGTTCGGTTCGTCTTACGTTCCAAGGTCAACACTCCCGATTTGATA
ACTATGCAGGTCCTGCATTTGATGATGAGTAA

Upstream 100 bases:

>100_bases
AATTCTTGCTGAAGAATAAAACAAACTCATAGGATCGGATTACAAACGGATGTTCTTGGGTATAATGAACGATCATTATT
AGTTATTGAGTGTAGTCATA

Downstream 100 bases:

>100_bases
TCACTAATGACTTACATGAAAGCAGCGACGGCGAGTATTGACTTAAACGCGCTTGAACATAACCTGAACCAGATAAAGTC
GAAGGCTCCTCATTGCAAGG

Product: replicative DNA helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 463; Mature: 463

Protein sequence:

>463_residues
MDTKSQKSANDQVDAIKVPPHSLEAEQSVIGGLLLDNERWDTVAEKVVAKDFYSRPHRLIFEAVKDILEESSPLDLITLS
EHLELREQLEEVGGFAYLADLAKNTPSAANINAYADIVAQRALVRSLIGVANEIADSGYDPQGRTSEELVDLAESKVFAI
AEGRASENEGPQNVDSILEKTLERIEILYKSPQDGVTGVDTGFNDLNKKTAGLQGSDLIIVAARPSMGKTTFAMNLCENA
AMKQDKPVLIFSLEMPAEQLMMRMLASLSRVDQTKIRTGQLDDEDWARISSSMGILMDKKNMYIDDSSGLTPTEVRSRAR
RIAREHDGISMIMIDYLQLMRVPSLSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQRADKRPVNSDLRESGSI
EQDADLIMFIYRDEVYNPDSSLKGIAEIILGKQRNGPIGSVRLTFQGQHSRFDNYAGPAFDDE

Sequences:

>Translated_463_residues
MDTKSQKSANDQVDAIKVPPHSLEAEQSVIGGLLLDNERWDTVAEKVVAKDFYSRPHRLIFEAVKDILEESSPLDLITLS
EHLELREQLEEVGGFAYLADLAKNTPSAANINAYADIVAQRALVRSLIGVANEIADSGYDPQGRTSEELVDLAESKVFAI
AEGRASENEGPQNVDSILEKTLERIEILYKSPQDGVTGVDTGFNDLNKKTAGLQGSDLIIVAARPSMGKTTFAMNLCENA
AMKQDKPVLIFSLEMPAEQLMMRMLASLSRVDQTKIRTGQLDDEDWARISSSMGILMDKKNMYIDDSSGLTPTEVRSRAR
RIAREHDGISMIMIDYLQLMRVPSLSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQRADKRPVNSDLRESGSI
EQDADLIMFIYRDEVYNPDSSLKGIAEIILGKQRNGPIGSVRLTFQGQHSRFDNYAGPAFDDE
>Mature_463_residues
MDTKSQKSANDQVDAIKVPPHSLEAEQSVIGGLLLDNERWDTVAEKVVAKDFYSRPHRLIFEAVKDILEESSPLDLITLS
EHLELREQLEEVGGFAYLADLAKNTPSAANINAYADIVAQRALVRSLIGVANEIADSGYDPQGRTSEELVDLAESKVFAI
AEGRASENEGPQNVDSILEKTLERIEILYKSPQDGVTGVDTGFNDLNKKTAGLQGSDLIIVAARPSMGKTTFAMNLCENA
AMKQDKPVLIFSLEMPAEQLMMRMLASLSRVDQTKIRTGQLDDEDWARISSSMGILMDKKNMYIDDSSGLTPTEVRSRAR
RIAREHDGISMIMIDYLQLMRVPSLSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQRADKRPVNSDLRESGSI
EQDADLIMFIYRDEVYNPDSSLKGIAEIILGKQRNGPIGSVRLTFQGQHSRFDNYAGPAFDDE

Specific function: Participates in initiation and elongation during chromosome replication; it exhibits DNA-dependent ATPase activity and contains distinct active sites for ATP binding, DNA binding, and interaction with dnaC protein, primase, and other prepriming proteins [

COG id: COG0305

COG function: function code L; Replicative DNA helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 SF4 helicase domain [H]

Homologues:

Organism=Escherichia coli, GI1790486, Length=452, Percent_Identity=79.8672566371681, Blast_Score=749, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR007692
- InterPro:   IPR007694
- InterPro:   IPR007693
- InterPro:   IPR016136 [H]

Pfam domain/function: PF00772 DnaB; PF03796 DnaB_C [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 51305; Mature: 51305

Theoretical pI: Translated: 4.54; Mature: 4.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDTKSQKSANDQVDAIKVPPHSLEAEQSVIGGLLLDNERWDTVAEKVVAKDFYSRPHRLI
CCCCCCCCCCCCCCEEECCCCCCCHHHHHHCCCEECCCHHHHHHHHHHHHHHHCCHHHHH
FEAVKDILEESSPLDLITLSEHLELREQLEEVGGFAYLADLAKNTPSAANINAYADIVAQ
HHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCHHHHHHHHH
RALVRSLIGVANEIADSGYDPQGRTSEELVDLAESKVFAIAEGRASENEGPQNVDSILEK
HHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCEEEEECCCCCCCCCCHHHHHHHHH
TLERIEILYKSPQDGVTGVDTGFNDLNKKTAGLQGSDLIIVAARPSMGKTTFAMNLCENA
HHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCCCCCCEEEEEECCCCCCHHHHHHHHHHH
AMKQDKPVLIFSLEMPAEQLMMRMLASLSRVDQTKIRTGQLDDEDWARISSSMGILMDKK
HCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCEEEECC
NMYIDDSSGLTPTEVRSRARRIAREHDGISMIMIDYLQLMRVPSLSDNRTLEIAEISRSL
CCEEECCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCEEHHHHHHHHH
KALAKELNVPVVALSQLNRSLEQRADKRPVNSDLRESGSIEQDADLIMFIYRDEVYNPDS
HHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCCCEEEEEEECCCCCCCH
SLKGIAEIILGKQRNGPIGSVRLTFQGQHSRFDNYAGPAFDDE
HHHHHHHHHHCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MDTKSQKSANDQVDAIKVPPHSLEAEQSVIGGLLLDNERWDTVAEKVVAKDFYSRPHRLI
CCCCCCCCCCCCCCEEECCCCCCCHHHHHHCCCEECCCHHHHHHHHHHHHHHHCCHHHHH
FEAVKDILEESSPLDLITLSEHLELREQLEEVGGFAYLADLAKNTPSAANINAYADIVAQ
HHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCHHHHHHHHH
RALVRSLIGVANEIADSGYDPQGRTSEELVDLAESKVFAIAEGRASENEGPQNVDSILEK
HHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCEEEEECCCCCCCCCCHHHHHHHHH
TLERIEILYKSPQDGVTGVDTGFNDLNKKTAGLQGSDLIIVAARPSMGKTTFAMNLCENA
HHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCCCCCCEEEEEECCCCCCHHHHHHHHHHH
AMKQDKPVLIFSLEMPAEQLMMRMLASLSRVDQTKIRTGQLDDEDWARISSSMGILMDKK
HCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCEEEECC
NMYIDDSSGLTPTEVRSRARRIAREHDGISMIMIDYLQLMRVPSLSDNRTLEIAEISRSL
CCEEECCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCEEHHHHHHHHH
KALAKELNVPVVALSQLNRSLEQRADKRPVNSDLRESGSIEQDADLIMFIYRDEVYNPDS
HHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCCCEEEEEEECCCCCCCH
SLKGIAEIILGKQRNGPIGSVRLTFQGQHSRFDNYAGPAFDDE
HHHHHHHHHHCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]