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The map label for this gene is rlmB [H]

Identifier: 218708332

GI number: 218708332

Start: 274227

End: 274967

Strand: Direct

Name: rlmB [H]

Synonym: VS_0279

Alternate gene names: 218708332

Gene position: 274227-274967 (Clockwise)

Preceding gene: 218708331

Following gene: 218708333

Centisome position: 8.31

GC content: 49.66

Gene sequence:

>741_bases
ATGAGTAACGAATTTATTTACGGTATTCACGCGGTGAAAGCCGTACTAGAAAAAGATCCAGCGCGTTTTATTGAAGCGTA
TGTACTGAAAGGTCGTCAAGACGATCGCCTGCTTCCATTACTGAATCAATTGCAGCAATTTGGTGTATCGATTCAACAAA
TGGGTCGTAAGCCGCTTGATGAAAAAGCACAAGGTGCGAATCACCAAGGTCTTATTGCTAAGGTGAAGCCTGCTAAGCAG
CTTAATGAAACTCACCTAGACGATATCCTTGCGCAGCACGAACAGCCTCTGCTGTTGGTTCTAGACGGCGTAACAGACCC
TCACAACCTAGGTGCTTGTCTGCGTAATGCGGATGCCGCGGGTGTTGCTGCTGTTATCGTACCGAAAGACCGCTCTTCGC
CGCTAACAGCAACGGTAAGCAAGGTAGCGTGTGGTGCGGCTGAGACCGTTCCTCTAGTACGTGTAACCAACCTCGCTCGA
ACAATGCGTGCACTGCAAGAACAGGGTGTATGGTTTGTGGGTACCGCAGGTGAAGCAACGCATGATATCTACCAAGCGAA
GTTAACAGGCCCTCTTGCTGTGGTTATGGGTGCTGAAGGTGACGGTATGCGTCGTCTAACGCGTGAAACCTGTGATGATC
TGATTAAGATCCCAATGGCAGGTAGCGTGTCGAGCCTGAACGTTTCAGTAGCATCGGGCATCTGTCTGTTCGAAGCGGTA
CGTCAGCGTTTAGCTCAATAG

Upstream 100 bases:

>100_bases
TATCAGGCGGAAGATGCATGCTCTCGAAATAAACAATACAGAGATGGGTGCATCGGGTTTGATGACATTCACCTCTGAGC
AATAAGAGTAACCAGAGACA

Downstream 100 bases:

>100_bases
CGTTAAGCTGAGTAAAAAGACAAAGCGACTTTAAGTCTATGAATTTATTAACGCTCACCACTTGCTGGTGGGCGTTTCTT
TTTAGGGCCTAGAGTGACAA

Product: 23S rRNA (guanosine-2'-O-)-methyltransferase

Products: NA

Alternate protein names: 23S rRNA Gm2251 2'-O-methyltransferase [H]

Number of amino acids: Translated: 246; Mature: 245

Protein sequence:

>246_residues
MSNEFIYGIHAVKAVLEKDPARFIEAYVLKGRQDDRLLPLLNQLQQFGVSIQQMGRKPLDEKAQGANHQGLIAKVKPAKQ
LNETHLDDILAQHEQPLLLVLDGVTDPHNLGACLRNADAAGVAAVIVPKDRSSPLTATVSKVACGAAETVPLVRVTNLAR
TMRALQEQGVWFVGTAGEATHDIYQAKLTGPLAVVMGAEGDGMRRLTRETCDDLIKIPMAGSVSSLNVSVASGICLFEAV
RQRLAQ

Sequences:

>Translated_246_residues
MSNEFIYGIHAVKAVLEKDPARFIEAYVLKGRQDDRLLPLLNQLQQFGVSIQQMGRKPLDEKAQGANHQGLIAKVKPAKQ
LNETHLDDILAQHEQPLLLVLDGVTDPHNLGACLRNADAAGVAAVIVPKDRSSPLTATVSKVACGAAETVPLVRVTNLAR
TMRALQEQGVWFVGTAGEATHDIYQAKLTGPLAVVMGAEGDGMRRLTRETCDDLIKIPMAGSVSSLNVSVASGICLFEAV
RQRLAQ
>Mature_245_residues
SNEFIYGIHAVKAVLEKDPARFIEAYVLKGRQDDRLLPLLNQLQQFGVSIQQMGRKPLDEKAQGANHQGLIAKVKPAKQL
NETHLDDILAQHEQPLLLVLDGVTDPHNLGACLRNADAAGVAAVIVPKDRSSPLTATVSKVACGAAETVPLVRVTNLART
MRALQEQGVWFVGTAGEATHDIYQAKLTGPLAVVMGAEGDGMRRLTRETCDDLIKIPMAGSVSSLNVSVASGICLFEAVR
QRLAQ

Specific function: Specifically methylates the ribose of guanosine 2251 in 23S rRNA [H]

COG id: COG0566

COG function: function code J; rRNA methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family. RlmB subfamily [H]

Homologues:

Organism=Homo sapiens, GI40068479, Length=208, Percent_Identity=32.6923076923077, Blast_Score=89, Evalue=5e-18,
Organism=Escherichia coli, GI1790623, Length=241, Percent_Identity=70.954356846473, Blast_Score=359, Evalue=1e-100,
Organism=Escherichia coli, GI1788935, Length=245, Percent_Identity=26.1224489795918, Blast_Score=82, Evalue=2e-17,
Organism=Escherichia coli, GI1790083, Length=159, Percent_Identity=33.3333333333333, Blast_Score=75, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI17555908, Length=254, Percent_Identity=25.5905511811024, Blast_Score=69, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6324775, Length=286, Percent_Identity=27.2727272727273, Blast_Score=88, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004441
- InterPro:   IPR001537
- InterPro:   IPR013123 [H]

Pfam domain/function: PF00588 SpoU_methylase; PF08032 SpoU_sub_bind [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 26476; Mature: 26345

Theoretical pI: Translated: 7.55; Mature: 7.55

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNEFIYGIHAVKAVLEKDPARFIEAYVLKGRQDDRLLPLLNQLQQFGVSIQQMGRKPLD
CCCCEEHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCHHHHHCCCCCC
EKAQGANHQGLIAKVKPAKQLNETHLDDILAQHEQPLLLVLDGVTDPHNLGACLRNADAA
HHHCCCCCCCEEEEECCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHCCCCC
GVAAVIVPKDRSSPLTATVSKVACGAAETVPLVRVTNLARTMRALQEQGVWFVGTAGEAT
CEEEEEEECCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEECCCCHH
HDIYQAKLTGPLAVVMGAEGDGMRRLTRETCDDLIKIPMAGSVSSLNVSVASGICLFEAV
HHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHH
RQRLAQ
HHHHCC
>Mature Secondary Structure 
SNEFIYGIHAVKAVLEKDPARFIEAYVLKGRQDDRLLPLLNQLQQFGVSIQQMGRKPLD
CCCEEHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCHHHHHCCCCCC
EKAQGANHQGLIAKVKPAKQLNETHLDDILAQHEQPLLLVLDGVTDPHNLGACLRNADAA
HHHCCCCCCCEEEEECCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHCCCCC
GVAAVIVPKDRSSPLTATVSKVACGAAETVPLVRVTNLARTMRALQEQGVWFVGTAGEAT
CEEEEEEECCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEECCCCHH
HDIYQAKLTGPLAVVMGAEGDGMRRLTRETCDDLIKIPMAGSVSSLNVSVASGICLFEAV
HHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHH
RQRLAQ
HHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]