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The map label for this gene is purA [H]

Identifier: 218708328

GI number: 218708328

Start: 267483

End: 268799

Strand: Direct

Name: purA [H]

Synonym: VS_0275

Alternate gene names: 218708328

Gene position: 267483-268799 (Clockwise)

Preceding gene: 218708326

Following gene: 218708329

Centisome position: 8.11

GC content: 45.94

Gene sequence:

>1317_bases
ATGGGAAATAACGTAGTCGTTCTAGGCACCCAATGGGGTGATGAAGGTAAAGGTAAAATCGTTGACCTTTTAACTGAAGA
TGCAAAATACGTGGTTCGCTACCAAGGCGGTCACAATGCAGGTCACACACTTGTAATTGACGGTGAAAAAACCGTTCTTC
ACTTAATTCCATCAGGCATCCTACGCAATAACGTTAAATGTGTTATTGGTAACGGTGTTGTATTATCGCCTGACGCACTT
CTTAAAGAGATGAAGCCTCTTGAAGAACGCGGTATCCCAGTGCGTGAGCGTCTTTTCATCTCTGAAGCTTGTCCTCTAAT
TCTTCCGTACCATATTGCTATCGACAACGCGCGTGAAATCGCTCGTGGCGCTAAAGCTATCGGTACAACAGGTCGTGGTA
TCGGTCCTGCTTACGAAGATAAAGTTGCTCGTCGCGGTCTACGCGTTGGCGACCTTTTCGATAAAGAAGCATTTGCTGAG
AAGCTAAAAGAAGTTATGGAATTCCACAACTTCCAACTAGAGCACTTCTACAAAGCTGAAACAGTAAGCTACGAAGAAGT
ACTTGAGCAAGCGATGAGCTACGCAGACATGTTAACTGCGATGGTTATCGACGTAACTGACGAACTAGACGCAGCACGTA
AGCGCGGCGACAAGATCATGTTTGAAGGTGCTCAAGGTACGCTACTAGATATCGACCACGGTACTTACCCATACGTAACG
TCTTCTAACACGACTGCTGGTGGTGTTGCTGCAGGTTCTGGTTTCGGTCCTCGTCACATCGGTTACATCCTTGGTATTAC
TAAGGCTTACTGTACTCGTGTTGGTTCAGGTCCATTCCCAACTGAGCTATACGATGGCCTTGAGAAGCAAGATCCAGTCG
GTAAGCACCTAGGCGATGTTGGTCACGAGTTTGGCGCAACTACTGGTCGTCTACGTCGTACAGGTTGGTTCGATGCTGTT
GCTATGCGTCGTGCAATCCAAATCAACTCTCTATCTGGTATGTGTCTAACTAAACTAGACGTTCTAGATGGCCTAGAAGA
ACTAAAAATCTGTACTGGTTACAAGATGAAAGATGGTTCTATCCTAGAAGTTTCTCCAATGGCTGCTGAGTCATTCGAAG
AAGCGACGCCAATCTACGAAACAATGCCTGGTTGGTCTGAAAACACATTTGGTGCTAAATCTATCGACGCGCTTCCACAA
GCTGCTCTAGATTACATCAAGCGTATCGAAGACCTAACTGGCGTTCCAATTGATATCGTATCAACTGGCCCAGATCGTAA
CGAAACTATCATCAAGGTTCACCCATACGGCGCATAA

Upstream 100 bases:

>100_bases
TTATTTTTGTACAGCGTTAACGTTTATGAACAAAAAGTGACTGCAAGAAGTGTGATTCAGTGCTAGAATCCATTTTTAAT
TAGCAACAGAAATTGGAAAG

Downstream 100 bases:

>100_bases
TGCCAGCTGAGTGATTACCACAGCACGAAGCGTACTGAGGTAATTAAAGTACTTTGATAATGAAATAGTTTTCTGAAAGC
CGACTTTATTAAGTCGGCTT

Product: adenylosuccinate synthetase

Products: NA

Alternate protein names: AMPSase; AdSS; IMP--aspartate ligase [H]

Number of amino acids: Translated: 438; Mature: 437

Protein sequence:

>438_residues
MGNNVVVLGTQWGDEGKGKIVDLLTEDAKYVVRYQGGHNAGHTLVIDGEKTVLHLIPSGILRNNVKCVIGNGVVLSPDAL
LKEMKPLEERGIPVRERLFISEACPLILPYHIAIDNAREIARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKEAFAE
KLKEVMEFHNFQLEHFYKAETVSYEEVLEQAMSYADMLTAMVIDVTDELDAARKRGDKIMFEGAQGTLLDIDHGTYPYVT
SSNTTAGGVAAGSGFGPRHIGYILGITKAYCTRVGSGPFPTELYDGLEKQDPVGKHLGDVGHEFGATTGRLRRTGWFDAV
AMRRAIQINSLSGMCLTKLDVLDGLEELKICTGYKMKDGSILEVSPMAAESFEEATPIYETMPGWSENTFGAKSIDALPQ
AALDYIKRIEDLTGVPIDIVSTGPDRNETIIKVHPYGA

Sequences:

>Translated_438_residues
MGNNVVVLGTQWGDEGKGKIVDLLTEDAKYVVRYQGGHNAGHTLVIDGEKTVLHLIPSGILRNNVKCVIGNGVVLSPDAL
LKEMKPLEERGIPVRERLFISEACPLILPYHIAIDNAREIARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKEAFAE
KLKEVMEFHNFQLEHFYKAETVSYEEVLEQAMSYADMLTAMVIDVTDELDAARKRGDKIMFEGAQGTLLDIDHGTYPYVT
SSNTTAGGVAAGSGFGPRHIGYILGITKAYCTRVGSGPFPTELYDGLEKQDPVGKHLGDVGHEFGATTGRLRRTGWFDAV
AMRRAIQINSLSGMCLTKLDVLDGLEELKICTGYKMKDGSILEVSPMAAESFEEATPIYETMPGWSENTFGAKSIDALPQ
AALDYIKRIEDLTGVPIDIVSTGPDRNETIIKVHPYGA
>Mature_437_residues
GNNVVVLGTQWGDEGKGKIVDLLTEDAKYVVRYQGGHNAGHTLVIDGEKTVLHLIPSGILRNNVKCVIGNGVVLSPDALL
KEMKPLEERGIPVRERLFISEACPLILPYHIAIDNAREIARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKEAFAEK
LKEVMEFHNFQLEHFYKAETVSYEEVLEQAMSYADMLTAMVIDVTDELDAARKRGDKIMFEGAQGTLLDIDHGTYPYVTS
SNTTAGGVAAGSGFGPRHIGYILGITKAYCTRVGSGPFPTELYDGLEKQDPVGKHLGDVGHEFGATTGRLRRTGWFDAVA
MRRAIQINSLSGMCLTKLDVLDGLEELKICTGYKMKDGSILEVSPMAAESFEEATPIYETMPGWSENTFGAKSIDALPQA
ALDYIKRIEDLTGVPIDIVSTGPDRNETIIKVHPYGA

Specific function: Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP [H]

COG id: COG0104

COG function: function code F; Adenylosuccinate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the adenylosuccinate synthetase family [H]

Homologues:

Organism=Homo sapiens, GI22748717, Length=431, Percent_Identity=42.6914153132251, Blast_Score=325, Evalue=7e-89,
Organism=Homo sapiens, GI34577063, Length=438, Percent_Identity=41.7808219178082, Blast_Score=317, Evalue=1e-86,
Organism=Homo sapiens, GI40316944, Length=402, Percent_Identity=40.547263681592, Blast_Score=281, Evalue=7e-76,
Organism=Escherichia coli, GI1790620, Length=438, Percent_Identity=75.5707762557078, Blast_Score=688, Evalue=0.0,
Organism=Caenorhabditis elegans, GI25146553, Length=436, Percent_Identity=42.2018348623853, Blast_Score=324, Evalue=5e-89,
Organism=Caenorhabditis elegans, GI25146555, Length=436, Percent_Identity=42.2018348623853, Blast_Score=324, Evalue=7e-89,
Organism=Saccharomyces cerevisiae, GI6324109, Length=441, Percent_Identity=40.8163265306122, Blast_Score=320, Evalue=4e-88,
Organism=Drosophila melanogaster, GI21356033, Length=431, Percent_Identity=41.2993039443155, Blast_Score=323, Evalue=1e-88,

Paralogues:

None

Copy number: 940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018220
- InterPro:   IPR001114 [H]

Pfam domain/function: PF00709 Adenylsucc_synt [H]

EC number: =6.3.4.4 [H]

Molecular weight: Translated: 47739; Mature: 47608

Theoretical pI: Translated: 5.13; Mature: 5.13

Prosite motif: PS01266 ADENYLOSUCCIN_SYN_1 ; PS00513 ADENYLOSUCCIN_SYN_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGNNVVVLGTQWGDEGKGKIVDLLTEDAKYVVRYQGGHNAGHTLVIDGEKTVLHLIPSGI
CCCCEEEEEECCCCCCCCCEEEEECCCCEEEEEECCCCCCCCEEEECCCCEEEEECCHHH
LRNNVKCVIGNGVVLSPDALLKEMKPLEERGIPVRERLFISEACPLILPYHIAIDNAREI
HHCCCEEEEECCEEECHHHHHHHHCCHHHCCCCHHHHHHHHHCCCEEEEEEEEECCHHHH
ARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKEAFAEKLKEVMEFHNFQLEHFYKAE
HHHHHHHCCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
TVSYEEVLEQAMSYADMLTAMVIDVTDELDAARKRGDKIMFEGAQGTLLDIDHGTYPYVT
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCEEECCCCCCCEEE
SSNTTAGGVAAGSGFGPRHIGYILGITKAYCTRVGSGPFPTELYDGLEKQDPVGKHLGDV
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCCCCHHHHHHHH
GHEFGATTGRLRRTGWFDAVAMRRAIQINSLSGMCLTKLDVLDGLEELKICTGYKMKDGS
HHHHCCCCCCHHHCCHHHHHHHHHHHEECCCCCHHHHHHHHHCCHHHHHHHCCCEECCCC
ILEVSPMAAESFEEATPIYETMPGWSENTFGAKSIDALPQAALDYIKRIEDLTGVPIDIV
EEEECCHHHHHHHHCCCHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEE
STGPDRNETIIKVHPYGA
ECCCCCCCEEEEEECCCC
>Mature Secondary Structure 
GNNVVVLGTQWGDEGKGKIVDLLTEDAKYVVRYQGGHNAGHTLVIDGEKTVLHLIPSGI
CCCEEEEEECCCCCCCCCEEEEECCCCEEEEEECCCCCCCCEEEECCCCEEEEECCHHH
LRNNVKCVIGNGVVLSPDALLKEMKPLEERGIPVRERLFISEACPLILPYHIAIDNAREI
HHCCCEEEEECCEEECHHHHHHHHCCHHHCCCCHHHHHHHHHCCCEEEEEEEEECCHHHH
ARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKEAFAEKLKEVMEFHNFQLEHFYKAE
HHHHHHHCCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
TVSYEEVLEQAMSYADMLTAMVIDVTDELDAARKRGDKIMFEGAQGTLLDIDHGTYPYVT
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCEEECCCCCCCEEE
SSNTTAGGVAAGSGFGPRHIGYILGITKAYCTRVGSGPFPTELYDGLEKQDPVGKHLGDV
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCCCCHHHHHHHH
GHEFGATTGRLRRTGWFDAVAMRRAIQINSLSGMCLTKLDVLDGLEELKICTGYKMKDGS
HHHHCCCCCCHHHCCHHHHHHHHHHHEECCCCCHHHHHHHHHCCHHHHHHHCCCEECCCC
ILEVSPMAAESFEEATPIYETMPGWSENTFGAKSIDALPQAALDYIKRIEDLTGVPIDIV
EEEECCHHHHHHHHCCCHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEE
STGPDRNETIIKVHPYGA
ECCCCCCCEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA