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The map label for this gene is waaA [H]

Identifier: 218708252

GI number: 218708252

Start: 184561

End: 185880

Strand: Direct

Name: waaA [H]

Synonym: VS_0189

Alternate gene names: 218708252

Gene position: 184561-185880 (Clockwise)

Preceding gene: 218708251

Following gene: 218708253

Centisome position: 5.59

GC content: 42.27

Gene sequence:

>1320_bases
ATGAGCATCGTCGTTCGTTGGGCCTATACCGCGCTACTTTTCCTTGTTAGCCCTATTCTGCTTTGGGGCTTATATCGCTC
CAAAGCAAACAAACCGCCTTTTGGTCATCGCTGGAAAGAGCATTTCGGATTTACACCGCCTATTAAGAATAGACAATCGG
GTGTGATTTGGATTCATGCCGTTTCAGTAGGGGAAGTGCTGGCGAGTAAAAAGTTAGTTGAACAACTGGCGATACAAAAC
CCAGATAAAAGAATACTGATCACTACAACAACGAGTACAGGCGCTGAGCAGGTCGAAAAGATGAGCGGTTCGATTAGCCA
TCGTTATATGCCTATTGATTTTAGTTGGTGTGTTCAACGTTTTATCAACCGCGTTCAGCCAGATAACATGCTCATTATTG
AAACGGAATTATGGCCAAACACCATTAACACCGTTGCTAAAAATGATATTCCGATGATATTAGTTAATGGACGATTATCC
GAAAAATCAGCCAGTAATTATCAAAAAATGAGTTCGTTAATTTGTCCGACAATTTCTAAGTTTAGTTTAATATTGACGGT
ACACTCTGATGATAAATCTCGATTTGAGTTACTAGGTGCACCGAGCGAAAAAGTCATTGTCACAGGCTCAATTAAGTACG
ATGTGTCGATTGATAATAATGTAATCGCACAAGGCGAGCATCTTAAAGATATTTTGGGGCAGGGTAGGCAGATTGTTGTT
GCCGCGAGCACTCATGCTGGTGAAGATGAACAAATTTTTAGAGCTTACCAACAAGCCAAGATCCAACTGCCAGAATTGCT
GTTAGTGATAGTGCCTCGTCATCCTGAACGGTTTGATTCAGTTGCTGAATTGGCCAAAAACCAAGGCTTAGTTTTGGTTC
GTCGAACCGAAGTTGTTGAGGAGTTACCGCTCGATGTCGACGTCTACTTGGGGGATACGATGGGAGAGCTTATGGTTATG
CTTTCTGCGAGCGATCTTGTCTTTATGGGAGGAAGCCTACTTGGAAAGAAAGTAGGAGGGCATAACTTCATCGAACCTGC
GCTGCTTCAAAAATACTGCTTAACAGGGCCGAGCTATTTCAATTTTGCAGACCTGGCGAATCAATTGATCGACTCGAGCG
CACTGTCTGTTGTAGCCGATGAGAATGAGCTTGCTTTACAAATGAACAGTGCATTATCCAAGCCTAAAGAGTTAATTGAA
AAAGGACGAATAGGCTATGGTATAGTTCAACAGAATCAAGGTGCGTTACAACAAACACTCCAATTGATACATCATTCTGC
CACCAATAATCAGTCAACCAATATTGAGACATCACCATGA

Upstream 100 bases:

>100_bases
CACCTAAAGTGCCTCACTGAGTTAATGCCGAGTAGAGTCATTAGTGCTATTGAGCAGTTTGATGTTATTTCCCAAGGCAA
AATATAAATAAGGTCGTTAT

Downstream 100 bases:

>100_bases
TCAAAACTTTTGTTATTCATGTAAGCAAAGGCTACGAAGAACGTAGAAAACATATAGATAAGCACCTGCCAGAAGTGGGC
ATCACTGATTACGAATATAT

Product: 3-deoxy-D-manno-octulosonic-acid transferase

Products: NA

Alternate protein names: KDO transferase [H]

Number of amino acids: Translated: 439; Mature: 438

Protein sequence:

>439_residues
MSIVVRWAYTALLFLVSPILLWGLYRSKANKPPFGHRWKEHFGFTPPIKNRQSGVIWIHAVSVGEVLASKKLVEQLAIQN
PDKRILITTTTSTGAEQVEKMSGSISHRYMPIDFSWCVQRFINRVQPDNMLIIETELWPNTINTVAKNDIPMILVNGRLS
EKSASNYQKMSSLICPTISKFSLILTVHSDDKSRFELLGAPSEKVIVTGSIKYDVSIDNNVIAQGEHLKDILGQGRQIVV
AASTHAGEDEQIFRAYQQAKIQLPELLLVIVPRHPERFDSVAELAKNQGLVLVRRTEVVEELPLDVDVYLGDTMGELMVM
LSASDLVFMGGSLLGKKVGGHNFIEPALLQKYCLTGPSYFNFADLANQLIDSSALSVVADENELALQMNSALSKPKELIE
KGRIGYGIVQQNQGALQQTLQLIHHSATNNQSTNIETSP

Sequences:

>Translated_439_residues
MSIVVRWAYTALLFLVSPILLWGLYRSKANKPPFGHRWKEHFGFTPPIKNRQSGVIWIHAVSVGEVLASKKLVEQLAIQN
PDKRILITTTTSTGAEQVEKMSGSISHRYMPIDFSWCVQRFINRVQPDNMLIIETELWPNTINTVAKNDIPMILVNGRLS
EKSASNYQKMSSLICPTISKFSLILTVHSDDKSRFELLGAPSEKVIVTGSIKYDVSIDNNVIAQGEHLKDILGQGRQIVV
AASTHAGEDEQIFRAYQQAKIQLPELLLVIVPRHPERFDSVAELAKNQGLVLVRRTEVVEELPLDVDVYLGDTMGELMVM
LSASDLVFMGGSLLGKKVGGHNFIEPALLQKYCLTGPSYFNFADLANQLIDSSALSVVADENELALQMNSALSKPKELIE
KGRIGYGIVQQNQGALQQTLQLIHHSATNNQSTNIETSP
>Mature_438_residues
SIVVRWAYTALLFLVSPILLWGLYRSKANKPPFGHRWKEHFGFTPPIKNRQSGVIWIHAVSVGEVLASKKLVEQLAIQNP
DKRILITTTTSTGAEQVEKMSGSISHRYMPIDFSWCVQRFINRVQPDNMLIIETELWPNTINTVAKNDIPMILVNGRLSE
KSASNYQKMSSLICPTISKFSLILTVHSDDKSRFELLGAPSEKVIVTGSIKYDVSIDNNVIAQGEHLKDILGQGRQIVVA
ASTHAGEDEQIFRAYQQAKIQLPELLLVIVPRHPERFDSVAELAKNQGLVLVRRTEVVEELPLDVDVYLGDTMGELMVML
SASDLVFMGGSLLGKKVGGHNFIEPALLQKYCLTGPSYFNFADLANQLIDSSALSVVADENELALQMNSALSKPKELIEK
GRIGYGIVQQNQGALQQTLQLIHHSATNNQSTNIETSP

Specific function: Essential step in lipopolysaccharides biosynthesis. Acts at transfer of 3-deoxy-D-mono octulonic acid (KDO) from CMP-KDO to a tetraacyldisaccharide 1,4'-bisphosphate precursor of lipid A (lipid IVA). Transfers two molecules of KDO to lipid IVA [H]

COG id: COG1519

COG function: function code M; 3-deoxy-D-manno-octulosonic-acid transferase

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 1 family [H]

Homologues:

Organism=Escherichia coli, GI1790064, Length=418, Percent_Identity=39.7129186602871, Blast_Score=295, Evalue=3e-81,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001296
- InterPro:   IPR007507 [H]

Pfam domain/function: PF00534 Glycos_transf_1; PF04413 Glycos_transf_N [H]

EC number: 2.-.-.-

Molecular weight: Translated: 48756; Mature: 48625

Theoretical pI: Translated: 6.92; Mature: 6.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIVVRWAYTALLFLVSPILLWGLYRSKANKPPFGHRWKEHFGFTPPIKNRQSGVIWIHA
CEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHCCCCCCCCCCCCCEEEEEE
VSVGEVLASKKLVEQLAIQNPDKRILITTTTSTGAEQVEKMSGSISHRYMPIDFSWCVQR
ECHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHCCCHHCEEECCCHHHHHHH
FINRVQPDNMLIIETELWPNTINTVAKNDIPMILVNGRLSEKSASNYQKMSSLICPTISK
HHHHCCCCCEEEEEECCCCCHHHHHHCCCCCEEEECCCCCCCHHHHHHHHHHHHCCCCCC
FSLILTVHSDDKSRFELLGAPSEKVIVTGSIKYDVSIDNNVIAQGEHLKDILGQGRQIVV
EEEEEEEECCCCCCEEEECCCCCCEEEEEEEEEEEEECCCEEECCHHHHHHHCCCCEEEE
AASTHAGEDEQIFRAYQQAKIQLPELLLVIVPRHPERFDSVAELAKNQGLVLVRRTEVVE
EECCCCCCHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHCCCEEEEEHHHHHH
ELPLDVDVYLGDTMGELMVMLSASDLVFMGGSLLGKKVGGHNFIEPALLQKYCLTGPSYF
HCCCCEEEEECCCHHHHHHHHCCCCEEEECCHHHHCCCCCCCCCCHHHHHHHHCCCCCCC
NFADLANQLIDSSALSVVADENELALQMNSALSKPKELIEKGRIGYGIVQQNQGALQQTL
CHHHHHHHHHCCCCEEEEECCCHHEEEHHHHHCCHHHHHHCCCCCEEEEECCCHHHHHHH
QLIHHSATNNQSTNIETSP
HHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure 
SIVVRWAYTALLFLVSPILLWGLYRSKANKPPFGHRWKEHFGFTPPIKNRQSGVIWIHA
EEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHCCCCCCCCCCCCCEEEEEE
VSVGEVLASKKLVEQLAIQNPDKRILITTTTSTGAEQVEKMSGSISHRYMPIDFSWCVQR
ECHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHCCCHHCEEECCCHHHHHHH
FINRVQPDNMLIIETELWPNTINTVAKNDIPMILVNGRLSEKSASNYQKMSSLICPTISK
HHHHCCCCCEEEEEECCCCCHHHHHHCCCCCEEEECCCCCCCHHHHHHHHHHHHCCCCCC
FSLILTVHSDDKSRFELLGAPSEKVIVTGSIKYDVSIDNNVIAQGEHLKDILGQGRQIVV
EEEEEEEECCCCCCEEEECCCCCCEEEEEEEEEEEEECCCEEECCHHHHHHHCCCCEEEE
AASTHAGEDEQIFRAYQQAKIQLPELLLVIVPRHPERFDSVAELAKNQGLVLVRRTEVVE
EECCCCCCHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHCCCEEEEEHHHHHH
ELPLDVDVYLGDTMGELMVMLSASDLVFMGGSLLGKKVGGHNFIEPALLQKYCLTGPSYF
HCCCCEEEEECCCHHHHHHHHCCCCEEEECCHHHHCCCCCCCCCCHHHHHHHHCCCCCCC
NFADLANQLIDSSALSVVADENELALQMNSALSKPKELIEKGRIGYGIVQQNQGALQQTL
CHHHHHHHHHCCCCEEEEECCCHHEEEHHHHHCCHHHHHHCCCCCEEEEECCCHHHHHHH
QLIHHSATNNQSTNIETSP
HHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]