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The map label for this gene is rph

Identifier: 218708240

GI number: 218708240

Start: 174446

End: 175162

Strand: Direct

Name: rph

Synonym: VS_0177

Alternate gene names: 218708240

Gene position: 174446-175162 (Clockwise)

Preceding gene: 218708238

Following gene: 218708241

Centisome position: 5.29

GC content: 49.51

Gene sequence:

>717_bases
ATGCGTCCAAATGACCGCGCTGTAGATCAAATTCGTCCAATTAAAATTACTCGTAACTACACTGCTTATGCTGAAGGTTC
TGTATTAGTTGAGTTCGGCAACACTAAAGTTCTATGTAATGCGACGGTAGAAGAAAACGTACCGCGTTGGTTGAAAGGCC
AAGGAAAGGGTTGGGTAACGGCTGAATACGGCATGCTTCCACGTGCGACGCACACTCGTAACCGTCGTGAAGCGGCGAGC
GGTAAGCAAGGTGGTCGTACGATGGAAATCCAACGTCTGATCGCTCGTAGCCTACGTGCTGTTGTTGACCTGAAAGTAAT
GGGTGAAATCATGATCACTGTCGATTGTGATGTTATCCAAGCAGACGGCGGTACTCGTACTGCTTCTATCTCAGGTGCAA
GCGTTGCAATGGCTGATGCTATCAACAGCCTACTAGCAAGCGGCAAACTGAAGAAGAACCCAATGAAAGGCCACGTAGCG
GCAGTTTCAGTGGGTATCGTTGGTGCACAAGCACTGTGTGACCTTGAGTACGTTGAAGACTCAGCAGCCGATACCGATAT
GAACGTTGTAATGACGGAAGACGGTAAGATGATTGAGATTCAAGGCACCGCAGAAGGCGAACCGTTCAGCCACGAAGAGC
TGATGCAGCTTTTAGCCCTGGCAAATAAGGGCATTGCCGATATCGTCGAAGCGCAGAAAGCTGCGTTGGCCGACTAG

Upstream 100 bases:

>100_bases
CTATATAATCTTGCCCCAATCAAAACTGTCTCACCCCTTTCTTAGGTGATGCACTCTTTCGATAGTAAATCACTTCAGAC
TGTTAATAAGGTAGATACCA

Downstream 100 bases:

>100_bases
TTTTTTAATAGCTCCCACATTGGGGGCTATTTTTTTATCTTCGTCGTATTCGTGCCTTCCGCGTTGTTAACTGCGCTTAC
TCACCCGAATCACTTACTAC

Product: ribonuclease PH

Products: NA

Alternate protein names: RNase PH; tRNA nucleotidyltransferase

Number of amino acids: Translated: 238; Mature: 238

Protein sequence:

>238_residues
MRPNDRAVDQIRPIKITRNYTAYAEGSVLVEFGNTKVLCNATVEENVPRWLKGQGKGWVTAEYGMLPRATHTRNRREAAS
GKQGGRTMEIQRLIARSLRAVVDLKVMGEIMITVDCDVIQADGGTRTASISGASVAMADAINSLLASGKLKKNPMKGHVA
AVSVGIVGAQALCDLEYVEDSAADTDMNVVMTEDGKMIEIQGTAEGEPFSHEELMQLLALANKGIADIVEAQKAALAD

Sequences:

>Translated_238_residues
MRPNDRAVDQIRPIKITRNYTAYAEGSVLVEFGNTKVLCNATVEENVPRWLKGQGKGWVTAEYGMLPRATHTRNRREAAS
GKQGGRTMEIQRLIARSLRAVVDLKVMGEIMITVDCDVIQADGGTRTASISGASVAMADAINSLLASGKLKKNPMKGHVA
AVSVGIVGAQALCDLEYVEDSAADTDMNVVMTEDGKMIEIQGTAEGEPFSHEELMQLLALANKGIADIVEAQKAALAD
>Mature_238_residues
MRPNDRAVDQIRPIKITRNYTAYAEGSVLVEFGNTKVLCNATVEENVPRWLKGQGKGWVTAEYGMLPRATHTRNRREAAS
GKQGGRTMEIQRLIARSLRAVVDLKVMGEIMITVDCDVIQADGGTRTASISGASVAMADAINSLLASGKLKKNPMKGHVA
AVSVGIVGAQALCDLEYVEDSAADTDMNVVMTEDGKMIEIQGTAEGEPFSHEELMQLLALANKGIADIVEAQKAALAD

Specific function: Phosphorolytic exoribonuclease that removes nucleotide residues following the -CCA terminus of tRNA and adds nucleotides to the ends of RNA molecules by using nucleoside diphosphates as substrates

COG id: COG0689

COG function: function code J; RNase PH

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase PH family

Homologues:

Organism=Escherichia coli, GI157672248, Length=211, Percent_Identity=75.8293838862559, Blast_Score=349, Evalue=7e-98,
Organism=Caenorhabditis elegans, GI71981632, Length=186, Percent_Identity=27.9569892473118, Blast_Score=66, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RNPH_VIBSL (B7VHJ7)

Other databases:

- EMBL:   FM954972
- RefSeq:   YP_002415861.1
- ProteinModelPortal:   B7VHJ7
- SMR:   B7VHJ7
- GeneID:   7159847
- GenomeReviews:   FM954972_GR
- KEGG:   vsp:VS_0177
- HOGENOM:   HBG737187
- OMA:   MLPRATG
- ProtClustDB:   PRK00173
- HAMAP:   MF_00564
- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR020568
- InterPro:   IPR002381
- InterPro:   IPR018336
- TIGRFAMs:   TIGR01966

Pfam domain/function: PF01138 RNase_PH; PF03725 RNase_PH_C; SSF55666 3_ExoRNase; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.7.56

Molecular weight: Translated: 25516; Mature: 25516

Theoretical pI: Translated: 5.41; Mature: 5.41

Prosite motif: PS01277 RIBONUCLEASE_PH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRPNDRAVDQIRPIKITRNYTAYAEGSVLVEFGNTKVLCNATVEENVPRWLKGQGKGWVT
CCCCCCCHHCCCCEEEECCEEEEECCEEEEEECCEEEEEECCHHHCCCHHHCCCCCEEEE
AEYGMLPRATHTRNRREAASGKQGGRTMEIQRLIARSLRAVVDLKVMGEIMITVDCDVIQ
EECCCCCCCCCCCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHEEECEEEEEEECEEEE
ADGGTRTASISGASVAMADAINSLLASGKLKKNPMKGHVAAVSVGIVGAQALCDLEYVED
CCCCCEEEECCCCHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEEHHHHHHHHHHHHHHC
SAADTDMNVVMTEDGKMIEIQGTAEGEPFSHEELMQLLALANKGIADIVEAQKAALAD
CCCCCCCEEEEECCCCEEEEEECCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHCCC
>Mature Secondary Structure
MRPNDRAVDQIRPIKITRNYTAYAEGSVLVEFGNTKVLCNATVEENVPRWLKGQGKGWVT
CCCCCCCHHCCCCEEEECCEEEEECCEEEEEECCEEEEEECCHHHCCCHHHCCCCCEEEE
AEYGMLPRATHTRNRREAASGKQGGRTMEIQRLIARSLRAVVDLKVMGEIMITVDCDVIQ
EECCCCCCCCCCCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHEEECEEEEEEECEEEE
ADGGTRTASISGASVAMADAINSLLASGKLKKNPMKGHVAAVSVGIVGAQALCDLEYVED
CCCCCEEEECCCCHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEEHHHHHHHHHHHHHHC
SAADTDMNVVMTEDGKMIEIQGTAEGEPFSHEELMQLLALANKGIADIVEAQKAALAD
CCCCCCCEEEEECCCCEEEEEECCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA