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The map label for this gene is hemN [H]

Identifier: 218708180

GI number: 218708180

Start: 106963

End: 108354

Strand: Direct

Name: hemN [H]

Synonym: VS_0116

Alternate gene names: 218708180

Gene position: 106963-108354 (Clockwise)

Preceding gene: 218708179

Following gene: 218708187

Centisome position: 3.24

GC content: 44.61

Gene sequence:

>1392_bases
ATGTCGAGCAAGCCAGTAACAACGAATCAGCAAATCGTTTGGGATCAAGAAATCTTAAACAAGTACAACTATTCGGGACC
TCGTTACACCTCATACCCAACTGCGTTGGAGTTTCATGAAGCGTTTACTGTCGCTGATTACGACATGGCGTGTACTCAAT
ATCCTGATCGTCCACTCTCTCTTTACGTGCATATCCCATTTTGTCACAAGCTTTGTTACTACTGTGGTTGTAATAAAGTC
ATTACTCGTCACTCGCATAAAGCGGATGAGTACTTGGATGTGATTGAACATGAAATCCGCCAGCGCGCCTCTTTATTGAA
TGGCCGTAAAGTGACTCAACTGCACTTCGGTGGTGGTACACCAACGTTCTTGACCAAAACACAAATCACTCGTTTGATGG
GCATTTTGCGTGAAGAGTTTAACTTCACCGCTGACGCTGAAATCAGTATTGAAGTTGACCCGCGTGAGATTGAGCTAGAC
GTACTTGACCACCTTCGAAATGAAGGCTTCAACCGCCTGAGCATTGGCGTGCAAGACTTTAATAAAGAAGTACAGAAGCT
GGTTAACCGTGAGCAAGATGAAGAGTTCATTATTGCAATGGTTCAACGTGCGACAGAGCTAGGCTTCCGTTCAACTAACC
TGGATTTGATCTACGGCCTACCAAAGCAGACTCAAGCTTTGTTCGCTGAAACATTGAAGCAAGTGTTAGAAATGAAGCCG
GGCCGTTTATCTGTATTTAACTACGCACACATGCCGCAACTGTTTGCTGCGCAGCGTAAGATAAAAGATGAAGACCTACC
TGAAGCGAAAGAGAAGATGGCTATCTTACAAGATACTATCGAGACGCTAACCGGTGCGGGTTACCAGTTCATTGGTATGG
ATCACTTCGCACTACCTGAAGATGAGCTAGCCGTAGCACAACGTGAAGGTATTCTGCATCGTAACTTCCAAGGCTACACG
ACCCAAGGTGAAGCTGACTTAATTGGCTTCGGTGTTTCTGCGATTTCTATGGTTGGTGATGCTTACGCACAAAATCAGAA
AGAGCTGAAGAAGTACTACGCTCAGGTGAATGACCTGCGTCATGCACTTTGGAAAGGTGTTGCTCTAGACAGTGACGACC
TTCTACGTCGTGAAGTGATCAAGCAGCTGATTTGTAACTTCAAGCTTGATAAAACAATGATCGAATCTGAGTTCTCGGTT
AACTTTAATCGCTACTTCAAAGAAGATTTAGAGCTTCTACAAACCTTTATCAACGATGAGTTGGTTGAAGTCGATGACAA
AGAAATCCGCGTGACTTTGCGTGGCCGTTTGCTGATCCGTAACATCTGTATGTGTTTTGATAAATACCTACGTGCCAAGG
CTCGCCAACAGCAATTCTCTCGCGTCATCTAA

Upstream 100 bases:

>100_bases
CTTACTGGTCGTGTGGCAAAATAACCCGTCTATATTTTAATGTATGTAAAATGATTTGAGAGACCTTAGGTTCTCGCGGA
TCTAAATTGGAAGTACCGCT

Downstream 100 bases:

>100_bases
TAACTCGATAGAGAATAGAAACATAAAAAATGCCAGCATCATGCTGGCATTTTTGTATCTGACTCATTCGGTTTCTGCCT
GATTTAGGCGAGGCTCTCTT

Product: coproporphyrinogen III oxidase

Products: NA

Alternate protein names: Coprogen oxidase; Coproporphyrinogenase [H]

Number of amino acids: Translated: 463; Mature: 462

Protein sequence:

>463_residues
MSSKPVTTNQQIVWDQEILNKYNYSGPRYTSYPTALEFHEAFTVADYDMACTQYPDRPLSLYVHIPFCHKLCYYCGCNKV
ITRHSHKADEYLDVIEHEIRQRASLLNGRKVTQLHFGGGTPTFLTKTQITRLMGILREEFNFTADAEISIEVDPREIELD
VLDHLRNEGFNRLSIGVQDFNKEVQKLVNREQDEEFIIAMVQRATELGFRSTNLDLIYGLPKQTQALFAETLKQVLEMKP
GRLSVFNYAHMPQLFAAQRKIKDEDLPEAKEKMAILQDTIETLTGAGYQFIGMDHFALPEDELAVAQREGILHRNFQGYT
TQGEADLIGFGVSAISMVGDAYAQNQKELKKYYAQVNDLRHALWKGVALDSDDLLRREVIKQLICNFKLDKTMIESEFSV
NFNRYFKEDLELLQTFINDELVEVDDKEIRVTLRGRLLIRNICMCFDKYLRAKARQQQFSRVI

Sequences:

>Translated_463_residues
MSSKPVTTNQQIVWDQEILNKYNYSGPRYTSYPTALEFHEAFTVADYDMACTQYPDRPLSLYVHIPFCHKLCYYCGCNKV
ITRHSHKADEYLDVIEHEIRQRASLLNGRKVTQLHFGGGTPTFLTKTQITRLMGILREEFNFTADAEISIEVDPREIELD
VLDHLRNEGFNRLSIGVQDFNKEVQKLVNREQDEEFIIAMVQRATELGFRSTNLDLIYGLPKQTQALFAETLKQVLEMKP
GRLSVFNYAHMPQLFAAQRKIKDEDLPEAKEKMAILQDTIETLTGAGYQFIGMDHFALPEDELAVAQREGILHRNFQGYT
TQGEADLIGFGVSAISMVGDAYAQNQKELKKYYAQVNDLRHALWKGVALDSDDLLRREVIKQLICNFKLDKTMIESEFSV
NFNRYFKEDLELLQTFINDELVEVDDKEIRVTLRGRLLIRNICMCFDKYLRAKARQQQFSRVI
>Mature_462_residues
SSKPVTTNQQIVWDQEILNKYNYSGPRYTSYPTALEFHEAFTVADYDMACTQYPDRPLSLYVHIPFCHKLCYYCGCNKVI
TRHSHKADEYLDVIEHEIRQRASLLNGRKVTQLHFGGGTPTFLTKTQITRLMGILREEFNFTADAEISIEVDPREIELDV
LDHLRNEGFNRLSIGVQDFNKEVQKLVNREQDEEFIIAMVQRATELGFRSTNLDLIYGLPKQTQALFAETLKQVLEMKPG
RLSVFNYAHMPQLFAAQRKIKDEDLPEAKEKMAILQDTIETLTGAGYQFIGMDHFALPEDELAVAQREGILHRNFQGYTT
QGEADLIGFGVSAISMVGDAYAQNQKELKKYYAQVNDLRHALWKGVALDSDDLLRREVIKQLICNFKLDKTMIESEFSVN
FNRYFKEDLELLQTFINDELVEVDDKEIRVTLRGRLLIRNICMCFDKYLRAKARQQQFSRVI

Specific function: Anaerobic transformation of coproporphyrinogen-III into protoporphyrinogen-IX [H]

COG id: COG0635

COG function: function code H; Coproporphyrinogen III oxidase and related Fe-S oxidoreductases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the anaerobic coproporphyrinogen-III oxidase family [H]

Homologues:

Organism=Homo sapiens, GI8922911, Length=247, Percent_Identity=27.1255060728745, Blast_Score=87, Evalue=5e-17,
Organism=Escherichia coli, GI87082341, Length=454, Percent_Identity=70.4845814977974, Blast_Score=681, Evalue=0.0,
Organism=Escherichia coli, GI1789325, Length=243, Percent_Identity=32.9218106995885, Blast_Score=137, Evalue=1e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR004558
- InterPro:   IPR010723
- InterPro:   IPR007197 [H]

Pfam domain/function: PF06969 HemN_C; PF04055 Radical_SAM [H]

EC number: =1.3.99.22 [H]

Molecular weight: Translated: 53636; Mature: 53505

Theoretical pI: Translated: 5.81; Mature: 5.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSKPVTTNQQIVWDQEILNKYNYSGPRYTSYPTALEFHEAFTVADYDMACTQYPDRPLS
CCCCCCCCCCCEEECHHHHHHHCCCCCCCCCCCCHHHHHHHHEEECCCHHHHCCCCCCEE
LYVHIPFCHKLCYYCGCNKVITRHSHKADEYLDVIEHEIRQRASLLNGRKVTQLHFGGGT
EEEECHHHHHHHHHHCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCC
PTFLTKTQITRLMGILREEFNFTADAEISIEVDPREIELDVLDHLRNEGFNRLSIGVQDF
CCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCHHHHHHHHHHHHCCCCEEECCHHHH
NKEVQKLVNREQDEEFIIAMVQRATELGFRSTNLDLIYGLPKQTQALFAETLKQVLEMKP
HHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCEEEECCCHHHHHHHHHHHHHHHHCCC
GRLSVFNYAHMPQLFAAQRKIKDEDLPEAKEKMAILQDTIETLTGAGYQFIGMDHFALPE
CCEEEECHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCC
DELAVAQREGILHRNFQGYTTQGEADLIGFGVSAISMVGDAYAQNQKELKKYYAQVNDLR
HHHHHHHHCCCHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
HALWKGVALDSDDLLRREVIKQLICNFKLDKTMIESEFSVNFNRYFKEDLELLQTFINDE
HHHHHCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHCCC
LVEVDDKEIRVTLRGRLLIRNICMCFDKYLRAKARQQQFSRVI
EEECCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SSKPVTTNQQIVWDQEILNKYNYSGPRYTSYPTALEFHEAFTVADYDMACTQYPDRPLS
CCCCCCCCCCEEECHHHHHHHCCCCCCCCCCCCHHHHHHHHEEECCCHHHHCCCCCCEE
LYVHIPFCHKLCYYCGCNKVITRHSHKADEYLDVIEHEIRQRASLLNGRKVTQLHFGGGT
EEEECHHHHHHHHHHCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCC
PTFLTKTQITRLMGILREEFNFTADAEISIEVDPREIELDVLDHLRNEGFNRLSIGVQDF
CCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCHHHHHHHHHHHHCCCCEEECCHHHH
NKEVQKLVNREQDEEFIIAMVQRATELGFRSTNLDLIYGLPKQTQALFAETLKQVLEMKP
HHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCEEEECCCHHHHHHHHHHHHHHHHCCC
GRLSVFNYAHMPQLFAAQRKIKDEDLPEAKEKMAILQDTIETLTGAGYQFIGMDHFALPE
CCEEEECHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCC
DELAVAQREGILHRNFQGYTTQGEADLIGFGVSAISMVGDAYAQNQKELKKYYAQVNDLR
HHHHHHHHCCCHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
HALWKGVALDSDDLLRREVIKQLICNFKLDKTMIESEFSVNFNRYFKEDLELLQTFINDE
HHHHHCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHCCC
LVEVDDKEIRVTLRGRLLIRNICMCFDKYLRAKARQQQFSRVI
EEECCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7768836; 8346018; 9278503 [H]