BacMap

Definition	Escherichia coli UMN026 chromosome, complete genome.
Accession	NC_011751
Length	5,202,090

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The map label for this gene is caiD [H]

Identifier: 229598881

GI number: 229598881

Start: 39813

End: 40598

Strand: Reverse

Name: caiD [H]

Synonym: ECUMN_0038

Alternate gene names: 229598881

Gene position: 40598-39813 (Counterclockwise)

Preceding gene: 218703298

Following gene: 218703296

Centisome position: 0.78

GC content: 54.58

Gene sequence:

>786_bases
ATGAGTGAATCATTACATCTGACCCGCAATGGATCAATTCTGGAAATTACCCTTGATCGCCCAAAAGCGAATGCTATTGA
TGCAAAAACCAGCTTTGAAATGGGCGAAGTATTTCTAAATTTCCGTGACGATCCGCAATTACGTGTCGCCATTATTACCG
GTGCCGGAGAGAAATTCTTTTCCGCGGGCTGGGATTTAAAAGCGGCAGCAGAAGGCGAAGCACCGGATGCTGACTTTGGC
CCGGGTGGTTTTGCGGGATTAACCGAAATTTTCAATCTCGACAAACCAGTTATCGCCGCTGTGAACGGCTATGCCTTTGG
CGGCGGCTTTGAACTGGCACTGGCGGCAGATTTTATTGTCTGTGCCGATAACGCCAGCTTCGCCCTGCCGGAAGCCAAAC
TGGGCATCGTTCCTGACAGCGGCGGCGTGCTGCGTCTGCCGAAGATCCTGCCGCCTGCCATCGTCAATGAAATGGTGATG
ACCGGCAGACGAATGGGCGCAGAAGAGGCGCTGCGTTGGGGGGTAGTCAACCGCGTGGTTAGCCAGGCGGAACTGATGGA
TAACGCCCGCGAACTGGCGCAGCAGCTGGTTAACAGCGCTCCGCTGGCGATTGCGGCGCTGAAAGAGATCTACCGCACCA
CCAGCGAAATGCCGGTAGAAGAAGCGTATCGCTATATTCGCAGCGGCGTGTTGAAACACTATCCGTCGGTTCTGCATTCG
GAAGATGCCATTGAAGGGCCGCTGGCGTTTGCCGAGAAGCGCGATCCGGTGTGGAAAGGACGTTAA

Upstream 100 bases:

>100_bases
GCAGGGAACTACCCTGCCAGCTAATAATCACGCTCTAAAATAATATGCATTTTTTGCAGAGATGTTGTCGTCCCTGAAAA
AGCAAAAATGGAGAAAAGGA

Downstream 100 bases:

>100_bases
CAACCGTGAGCTATTACGCCTTTGAGGGGTTAATTCCGGTGGTTCACCCGACGGCGTTTGTCCATCCCAGTGCAGTCTTG
ATTGGCGATGTGATTGTGGG

Product: carnitinyl-CoA dehydratase

Products: L-Carnitine.; L-carnitinyl-CoA [C]

Alternate protein names: Crotonobetainyl-CoA hydratase [H]

Number of amino acids: Translated: 261; Mature: 260

Protein sequence:

>261_residues
MSESLHLTRNGSILEITLDRPKANAIDAKTSFEMGEVFLNFRDDPQLRVAIITGAGEKFFSAGWDLKAAAEGEAPDADFG
PGGFAGLTEIFNLDKPVIAAVNGYAFGGGFELALAADFIVCADNASFALPEAKLGIVPDSGGVLRLPKILPPAIVNEMVM
TGRRMGAEEALRWGVVNRVVSQAELMDNARELAQQLVNSAPLAIAALKEIYRTTSEMPVEEAYRYIRSGVLKHYPSVLHS
EDAIEGPLAFAEKRDPVWKGR

Sequences:

>Translated_261_residues
MSESLHLTRNGSILEITLDRPKANAIDAKTSFEMGEVFLNFRDDPQLRVAIITGAGEKFFSAGWDLKAAAEGEAPDADFG
PGGFAGLTEIFNLDKPVIAAVNGYAFGGGFELALAADFIVCADNASFALPEAKLGIVPDSGGVLRLPKILPPAIVNEMVM
TGRRMGAEEALRWGVVNRVVSQAELMDNARELAQQLVNSAPLAIAALKEIYRTTSEMPVEEAYRYIRSGVLKHYPSVLHS
EDAIEGPLAFAEKRDPVWKGR
>Mature_260_residues
SESLHLTRNGSILEITLDRPKANAIDAKTSFEMGEVFLNFRDDPQLRVAIITGAGEKFFSAGWDLKAAAEGEAPDADFGP
GGFAGLTEIFNLDKPVIAAVNGYAFGGGFELALAADFIVCADNASFALPEAKLGIVPDSGGVLRLPKILPPAIVNEMVMT
GRRMGAEEALRWGVVNRVVSQAELMDNARELAQQLVNSAPLAIAALKEIYRTTSEMPVEEAYRYIRSGVLKHYPSVLHSE
DAIEGPLAFAEKRDPVWKGR

Specific function: Catalyzes the reversible dehydration of L-carnitinyl-CoA to crotonobetainyl-CoA [H]

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=252, Percent_Identity=33.3333333333333, Blast_Score=137, Evalue=9e-33,
Organism=Homo sapiens, GI70995211, Length=269, Percent_Identity=33.8289962825279, Blast_Score=117, Evalue=8e-27,
Organism=Homo sapiens, GI31542718, Length=249, Percent_Identity=30.1204819277108, Blast_Score=96, Evalue=4e-20,
Organism=Homo sapiens, GI68989263, Length=187, Percent_Identity=33.6898395721925, Blast_Score=89, Evalue=3e-18,
Organism=Homo sapiens, GI37594471, Length=227, Percent_Identity=28.6343612334802, Blast_Score=87, Evalue=1e-17,
Organism=Homo sapiens, GI37594469, Length=227, Percent_Identity=28.6343612334802, Blast_Score=87, Evalue=1e-17,
Organism=Homo sapiens, GI4502327, Length=231, Percent_Identity=32.9004329004329, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI150378541, Length=254, Percent_Identity=29.9212598425197, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI45643119, Length=225, Percent_Identity=28, Blast_Score=75, Evalue=5e-14,
Organism=Homo sapiens, GI260275230, Length=225, Percent_Identity=28, Blast_Score=75, Evalue=5e-14,
Organism=Homo sapiens, GI260274832, Length=225, Percent_Identity=28, Blast_Score=75, Evalue=5e-14,
Organism=Homo sapiens, GI20127408, Length=173, Percent_Identity=27.1676300578035, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI157694516, Length=218, Percent_Identity=26.1467889908257, Blast_Score=71, Evalue=8e-13,
Organism=Homo sapiens, GI213417737, Length=218, Percent_Identity=26.1467889908257, Blast_Score=71, Evalue=9e-13,
Organism=Homo sapiens, GI221136756, Length=136, Percent_Identity=28.6764705882353, Blast_Score=69, Evalue=6e-12,
Organism=Homo sapiens, GI221136753, Length=136, Percent_Identity=28.6764705882353, Blast_Score=69, Evalue=6e-12,
Organism=Homo sapiens, GI221307494, Length=136, Percent_Identity=28.6764705882353, Blast_Score=69, Evalue=6e-12,
Organism=Homo sapiens, GI157694520, Length=182, Percent_Identity=28.021978021978, Blast_Score=67, Evalue=2e-11,
Organism=Escherichia coli, GI221142681, Length=261, Percent_Identity=99.6168582375479, Blast_Score=527, Evalue=1e-151,
Organism=Escherichia coli, GI1787659, Length=264, Percent_Identity=36.3636363636364, Blast_Score=140, Evalue=7e-35,
Organism=Escherichia coli, GI1788682, Length=197, Percent_Identity=35.5329949238579, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI87082183, Length=218, Percent_Identity=31.1926605504587, Blast_Score=101, Evalue=6e-23,
Organism=Escherichia coli, GI1787660, Length=261, Percent_Identity=29.8850574712644, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1788597, Length=248, Percent_Identity=29.4354838709677, Blast_Score=92, Evalue=2e-20,
Organism=Escherichia coli, GI1790281, Length=166, Percent_Identity=33.1325301204819, Blast_Score=89, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI25145438, Length=258, Percent_Identity=34.1085271317829, Blast_Score=141, Evalue=3e-34,
Organism=Caenorhabditis elegans, GI17554946, Length=247, Percent_Identity=31.5789473684211, Blast_Score=131, Evalue=3e-31,
Organism=Caenorhabditis elegans, GI17560910, Length=249, Percent_Identity=28.5140562248996, Blast_Score=119, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI17540714, Length=202, Percent_Identity=32.1782178217822, Blast_Score=103, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI17534483, Length=257, Percent_Identity=27.6264591439689, Blast_Score=98, Evalue=5e-21,
Organism=Caenorhabditis elegans, GI17536985, Length=265, Percent_Identity=25.2830188679245, Blast_Score=92, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17549921, Length=218, Percent_Identity=28.4403669724771, Blast_Score=91, Evalue=9e-19,
Organism=Caenorhabditis elegans, GI17558304, Length=174, Percent_Identity=29.3103448275862, Blast_Score=87, Evalue=8e-18,
Organism=Caenorhabditis elegans, GI17540306, Length=253, Percent_Identity=28.8537549407115, Blast_Score=86, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17535521, Length=220, Percent_Identity=28.1818181818182, Blast_Score=82, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI25144276, Length=187, Percent_Identity=28.8770053475936, Blast_Score=78, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI17508953, Length=187, Percent_Identity=28.8770053475936, Blast_Score=77, Evalue=6e-15,
Organism=Caenorhabditis elegans, GI17508951, Length=187, Percent_Identity=28.8770053475936, Blast_Score=77, Evalue=7e-15,
Organism=Caenorhabditis elegans, GI25144157, Length=214, Percent_Identity=27.1028037383178, Blast_Score=69, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI25144160, Length=214, Percent_Identity=27.1028037383178, Blast_Score=69, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6320241, Length=182, Percent_Identity=26.3736263736264, Blast_Score=65, Evalue=8e-12,
Organism=Drosophila melanogaster, GI20129971, Length=240, Percent_Identity=35, Blast_Score=138, Evalue=4e-33,
Organism=Drosophila melanogaster, GI24653477, Length=240, Percent_Identity=35, Blast_Score=138, Evalue=4e-33,
Organism=Drosophila melanogaster, GI24653139, Length=272, Percent_Identity=36.0294117647059, Blast_Score=128, Evalue=4e-30,
Organism=Drosophila melanogaster, GI19922422, Length=265, Percent_Identity=28.6792452830189, Blast_Score=101, Evalue=6e-22,
Organism=Drosophila melanogaster, GI21357171, Length=220, Percent_Identity=30.9090909090909, Blast_Score=94, Evalue=7e-20,
Organism=Drosophila melanogaster, GI19920382, Length=230, Percent_Identity=28.2608695652174, Blast_Score=91, Evalue=6e-19,
Organism=Drosophila melanogaster, GI24650670, Length=189, Percent_Identity=33.3333333333333, Blast_Score=85, Evalue=5e-17,
Organism=Drosophila melanogaster, GI24654903, Length=187, Percent_Identity=29.9465240641711, Blast_Score=82, Evalue=4e-16,
Organism=Drosophila melanogaster, GI28571730, Length=176, Percent_Identity=28.9772727272727, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI28571729, Length=176, Percent_Identity=28.9772727272727, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI19921000, Length=186, Percent_Identity=29.5698924731183, Blast_Score=68, Evalue=5e-12,
Organism=Drosophila melanogaster, GI24583077, Length=186, Percent_Identity=29.5698924731183, Blast_Score=68, Evalue=7e-12,
Organism=Drosophila melanogaster, GI24583079, Length=186, Percent_Identity=29.5698924731183, Blast_Score=68, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022852
- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: 4.2.1.- [C]

Molecular weight: Translated: 28176; Mature: 28045

Theoretical pI: Translated: 4.65; Mature: 4.65

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSESLHLTRNGSILEITLDRPKANAIDAKTSFEMGEVFLNFRDDPQLRVAIITGAGEKFF
CCCCEEEECCCCEEEEEECCCCCCCCCCHHHHHHHHHEEECCCCCCEEEEEEECCCHHHH
SAGWDLKAAAEGEAPDADFGPGGFAGLTEIFNLDKPVIAAVNGYAFGGGFELALAADFIV
HCCCCCEECCCCCCCCCCCCCCCHHHHHHHHCCCCCCEEEECCEEECCCEEEEEEEEEEE
CADNASFALPEAKLGIVPDSGGVLRLPKILPPAIVNEMVMTGRRMGAEEALRWGVVNRVV
EECCCCEECCCCCEEEECCCCCEEECCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
SQAELMDNARELAQQLVNSAPLAIAALKEIYRTTSEMPVEEAYRYIRSGVLKHYPSVLHS
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCC
EDAIEGPLAFAEKRDPVWKGR
CCCCCCCHHHHHCCCCCCCCC
>Mature Secondary Structure 
SESLHLTRNGSILEITLDRPKANAIDAKTSFEMGEVFLNFRDDPQLRVAIITGAGEKFF
CCCEEEECCCCEEEEEECCCCCCCCCCHHHHHHHHHEEECCCCCCEEEEEEECCCHHHH
SAGWDLKAAAEGEAPDADFGPGGFAGLTEIFNLDKPVIAAVNGYAFGGGFELALAADFIV
HCCCCCEECCCCCCCCCCCCCCCHHHHHHHHCCCCCCEEEECCEEECCCEEEEEEEEEEE
CADNASFALPEAKLGIVPDSGGVLRLPKILPPAIVNEMVMTGRRMGAEEALRWGVVNRVV
EECCCCEECCCCCEEEECCCCCEEECCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
SQAELMDNARELAQQLVNSAPLAIAALKEIYRTTSEMPVEEAYRYIRSGVLKHYPSVLHS
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCC
EDAIEGPLAFAEKRDPVWKGR
CCCCCCCHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: D-Carnitine; D-carnitinyl-CoA [C]

Specific reaction: D-Carnitine = L-Carnitine. D-carnitinyl-CoA = L-carnitinyl-CoA [C]

General reaction: Isomerases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA