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The map label for this gene is otsB [H]

Identifier: 218705395

GI number: 218705395

Start: 2215021

End: 2215821

Strand: Reverse

Name: otsB [H]

Synonym: ECUMN_2192

Alternate gene names: 218705395

Gene position: 2215821-2215021 (Counterclockwise)

Preceding gene: 218705396

Following gene: 218705394

Centisome position: 42.59

GC content: 51.44

Gene sequence:

>801_bases
GTGACAGAACCGTTAACCGAAACCCCTGAACTATCCGCGAAATATGCCTGGTTTTTTGATCTCGATGGAACGCTGGCGGA
AATCAAACCGCATCCCGATCAGGTCGTCGTGCCTGACAATATTCTGCAAGGACTTCAGCTACTGGCAACCGCAAGTGATG
GTGCATTGGCATTGATATCAGGGCGCTCAATGGTGGAGCTTGACGCACTGGCAAAACCTTATCGCTTCCCATTAGCGGGC
GTGCATGGGGCGGAGCGCCGTGACATCAATGGTAAAACGCATATCGTTCATCTGCCGGATGCGATTGCACGTGATATTAG
CGTGCAACTGCATACGGCTATCGCCCAGTTTCCCGGTGCGGAGCTGGAGACGAAAGGGATGGCCTTTGCGCTGCATTATC
GTCAGGCTCCGCAGCATGAAGACGCATTAATGACATTAGCGCAACGTATTACTCAGATCTGGCCACAAATGGCGTTACAG
CAGGGAAAGTGTGTTGTCGAGATCAAACCGCGAGGCACCAGTAAAGGTGAGGCGATTGCTGCCTTTATGCAGGAAGCTCC
CTTTATCGGGCGAACGCCGGTATTTCTGGGCGATGATTTAACCGATGAATCTGGCTTCGCTGTCGTTAACCGGCTGGGCG
GAATGTCAGTAAAAATTGGCACAGGCGCAACTCAGGCATCATGGCGACTGGCGGGCGTACCGGATGTCTGGAGCTGGCTT
GAAATGATAACCACCGCATTACAACAAAAAAGAGAAAATAACAGGAGTGATGACTATGAGTCGTTTAGTCGTAGTATCTA
A

Upstream 100 bases:

>100_bases
CTTCGTTACAAATGGCGACCCCCGTCACACTGTCTATACTTACATGTCTGTAAAGCGCGTTCTGCGCAACACAATAAGAA
AAGAGAAGGAGGAGAACCGG

Downstream 100 bases:

>100_bases
CCGGATTGCACCACCAGATGAGCACGCCGCCAGTGCCGGTGGCCTTGCCGTTGGCATTCTGGGGGCACTGAAAGCCGCAG
GTGGACTGTGGTTTGGCTGG

Product: trehalose-6-phosphate phosphatase

Products: NA

Alternate protein names: TPP; Osmoregulatory trehalose synthesis protein B; Trehalose 6-phosphate phosphatase; Trehalose-phosphatase [H]

Number of amino acids: Translated: 266; Mature: 265

Protein sequence:

>266_residues
MTEPLTETPELSAKYAWFFDLDGTLAEIKPHPDQVVVPDNILQGLQLLATASDGALALISGRSMVELDALAKPYRFPLAG
VHGAERRDINGKTHIVHLPDAIARDISVQLHTAIAQFPGAELETKGMAFALHYRQAPQHEDALMTLAQRITQIWPQMALQ
QGKCVVEIKPRGTSKGEAIAAFMQEAPFIGRTPVFLGDDLTDESGFAVVNRLGGMSVKIGTGATQASWRLAGVPDVWSWL
EMITTALQQKRENNRSDDYESFSRSI

Sequences:

>Translated_266_residues
MTEPLTETPELSAKYAWFFDLDGTLAEIKPHPDQVVVPDNILQGLQLLATASDGALALISGRSMVELDALAKPYRFPLAG
VHGAERRDINGKTHIVHLPDAIARDISVQLHTAIAQFPGAELETKGMAFALHYRQAPQHEDALMTLAQRITQIWPQMALQ
QGKCVVEIKPRGTSKGEAIAAFMQEAPFIGRTPVFLGDDLTDESGFAVVNRLGGMSVKIGTGATQASWRLAGVPDVWSWL
EMITTALQQKRENNRSDDYESFSRSI
>Mature_265_residues
TEPLTETPELSAKYAWFFDLDGTLAEIKPHPDQVVVPDNILQGLQLLATASDGALALISGRSMVELDALAKPYRFPLAGV
HGAERRDINGKTHIVHLPDAIARDISVQLHTAIAQFPGAELETKGMAFALHYRQAPQHEDALMTLAQRITQIWPQMALQQ
GKCVVEIKPRGTSKGEAIAAFMQEAPFIGRTPVFLGDDLTDESGFAVVNRLGGMSVKIGTGATQASWRLAGVPDVWSWLE
MITTALQQKRENNRSDDYESFSRSI

Specific function: Removes the phosphate from trehalose 6-phosphate to produce free trehalose [H]

COG id: COG1877

COG function: function code G; Trehalose-6-phosphatase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trehalose phosphatase family [H]

Homologues:

Organism=Escherichia coli, GI1788207, Length=266, Percent_Identity=98.8721804511278, Blast_Score=543, Evalue=1e-156,
Organism=Drosophila melanogaster, GI19920676, Length=260, Percent_Identity=26.5384615384615, Blast_Score=75, Evalue=5e-14,
Organism=Drosophila melanogaster, GI221473368, Length=239, Percent_Identity=29.7071129707113, Blast_Score=69, Evalue=3e-12,
Organism=Drosophila melanogaster, GI20129307, Length=239, Percent_Identity=29.7071129707113, Blast_Score=69, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24582490, Length=239, Percent_Identity=29.7071129707113, Blast_Score=69, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023214
- InterPro:   IPR006379
- InterPro:   IPR003337 [H]

Pfam domain/function: PF02358 Trehalose_PPase [H]

EC number: =3.1.3.12 [H]

Molecular weight: Translated: 29162; Mature: 29030

Theoretical pI: Translated: 5.34; Mature: 5.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEPLTETPELSAKYAWFFDLDGTLAEIKPHPDQVVVPDNILQGLQLLATASDGALALIS
CCCCCCCCCCCCEEEEEEEECCCCCEECCCCCCCEECCHHHHHHHHHHHCCCCCEEEEEC
GRSMVELDALAKPYRFPLAGVHGAERRDINGKTHIVHLPDAIARDISVQLHTAIAQFPGA
CCCEEEHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHCCCC
ELETKGMAFALHYRQAPQHEDALMTLAQRITQIWPQMALQQGKCVVEIKPRGTSKGEAIA
CCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHH
AFMQEAPFIGRTPVFLGDDLTDESGFAVVNRLGGMSVKIGTGATQASWRLAGVPDVWSWL
HHHHHCCCCCCCCEEECCCCCCCCCHHHHHHCCCCEEEECCCCCCCCEEECCCCHHHHHH
EMITTALQQKRENNRSDDYESFSRSI
HHHHHHHHHHHHCCCCCCHHHHHCCC
>Mature Secondary Structure 
TEPLTETPELSAKYAWFFDLDGTLAEIKPHPDQVVVPDNILQGLQLLATASDGALALIS
CCCCCCCCCCCEEEEEEEECCCCCEECCCCCCCEECCHHHHHHHHHHHCCCCCEEEEEC
GRSMVELDALAKPYRFPLAGVHGAERRDINGKTHIVHLPDAIARDISVQLHTAIAQFPGA
CCCEEEHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHCCCC
ELETKGMAFALHYRQAPQHEDALMTLAQRITQIWPQMALQQGKCVVEIKPRGTSKGEAIA
CCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHH
AFMQEAPFIGRTPVFLGDDLTDESGFAVVNRLGGMSVKIGTGATQASWRLAGVPDVWSWL
HHHHHCCCCCCCCEEECCCCCCCCCHHHHHHCCCCEEEECCCCCCCCEEECCCCHHHHHH
EMITTALQQKRENNRSDDYESFSRSI
HHHHHHHHHHHHCCCCCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8045430; 9097040; 9278503 [H]