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The map label for this gene is torY [H]

Identifier: 218705374

GI number: 218705374

Start: 2191975

End: 2193075

Strand: Reverse

Name: torY [H]

Synonym: ECUMN_2171

Alternate gene names: 218705374

Gene position: 2193075-2191975 (Counterclockwise)

Preceding gene: 218705375

Following gene: 218705373

Centisome position: 42.16

GC content: 47.68

Gene sequence:

>1101_bases
ATGCGAGGGAAAAAACGCATTGGGTTAATGTTTTTGCTGATAGCGGTTGTGGTTGGTGGCGGCGGGTTATTGCTGGCGCA
AAAAGCCTTACATAAAACGTCGGATACAGCATTTTGCCTTTCCTGCCACTCGATGAGTAAACCTTTTGAGGAATATCAGG
GAACTGTCCACTTTTCGAACCAGAAAGGGATACGTGCGGAATGTGCCGATTGCCATATTCCAAAGTCAGGGATGGATTAT
TTATTTGCTAAATTAAAAGCTTCTAAAGATATTTATCATGAATTTGTTAGCGGCAAAATAGACAGTGACGATAAATTCGA
AGCGCATCGTCAGGAAATGGCCGAAACAGTATGGAAAGAATTAAAAGCAACTGATTCTGCAACGTGTCGTAGTTGCCATT
CTTTTGATGCCATGGATATTGCCTCGCAAAGTGAATCTGCGCAGAAAATGCATACCAAAGCGCAAAAGGACGGCGAAACC
TGTATCGATTGTCATAAAGGCATTGCCCATTTTCCACCAGAAATAAAAATGGATGACAACGCGGCGCATGAGCTGGAAAG
TCAGGCCGCTACTTCAGTTACCAATGGCGCACATATTTATCCTTTCAAACCTTCCCGCATAGGCGAACTGGCCACTGTGA
ATCCTGGTACCGATCTCACCGTCGTTGATGCCAGTGGCAAACAGCCGATCGTTCTGTTGCAGGGTTATCAAATGCAGGGC
AGTGAAAACACGCTCTACCTGGCGGCAGGTCAACGGCTGGCGCTAGCCACATTAAGTGAAGAAGGTATCAAGGCGCTGAC
GGTAAACGGGGAATGGCAGGCTGACGAGTACGGCAATCAATGGCGTCAGGCGTCTATACAGGGTGTGCTTACCGATCCCG
CATTAGCGGACCGTAAACCGCTATGGCAATACGCTGAAAAACTTGACGATACCTATTGCGCTGGTTGCCATGCGCCTATT
GCCGCTGACCATTACACCGTCAATGCCTGGCCGTCCATTGCCAAAGGAATGGGGGCGCGAACCAGCATGAGCGAAAATGA
ACTGGACATTTTGACGCGGTACTTCCAGTACAACGCCAAAGATATTAGCGAAAAACAGTGA

Upstream 100 bases:

>100_bases
TTATTTCAGGCAGTGTTGTGCCGAATTATGCAAGCGGTCAATTCGTTGTATATTTAATTATACAATGATTTCGGTGTTCT
GGAATTAATTAGAGGAATCT

Downstream 100 bases:

>100_bases
TCCTCGTTGATCAGGGAGGAAGTTATGACATTAACAAGACGTGAATTTATTAAACACAGCGGTATTGCCGCTGGGGCGCT
GGTGGTGACATCGGCTGCAC

Product: TMAO reductase III (TorYZ), cytochrome c-type subunit

Products: H2O; trimethylamine; NAD [C]

Alternate protein names: NA

Number of amino acids: Translated: 366; Mature: 366

Protein sequence:

>366_residues
MRGKKRIGLMFLLIAVVVGGGGLLLAQKALHKTSDTAFCLSCHSMSKPFEEYQGTVHFSNQKGIRAECADCHIPKSGMDY
LFAKLKASKDIYHEFVSGKIDSDDKFEAHRQEMAETVWKELKATDSATCRSCHSFDAMDIASQSESAQKMHTKAQKDGET
CIDCHKGIAHFPPEIKMDDNAAHELESQAATSVTNGAHIYPFKPSRIGELATVNPGTDLTVVDASGKQPIVLLQGYQMQG
SENTLYLAAGQRLALATLSEEGIKALTVNGEWQADEYGNQWRQASIQGVLTDPALADRKPLWQYAEKLDDTYCAGCHAPI
AADHYTVNAWPSIAKGMGARTSMSENELDILTRYFQYNAKDISEKQ

Sequences:

>Translated_366_residues
MRGKKRIGLMFLLIAVVVGGGGLLLAQKALHKTSDTAFCLSCHSMSKPFEEYQGTVHFSNQKGIRAECADCHIPKSGMDY
LFAKLKASKDIYHEFVSGKIDSDDKFEAHRQEMAETVWKELKATDSATCRSCHSFDAMDIASQSESAQKMHTKAQKDGET
CIDCHKGIAHFPPEIKMDDNAAHELESQAATSVTNGAHIYPFKPSRIGELATVNPGTDLTVVDASGKQPIVLLQGYQMQG
SENTLYLAAGQRLALATLSEEGIKALTVNGEWQADEYGNQWRQASIQGVLTDPALADRKPLWQYAEKLDDTYCAGCHAPI
AADHYTVNAWPSIAKGMGARTSMSENELDILTRYFQYNAKDISEKQ
>Mature_366_residues
MRGKKRIGLMFLLIAVVVGGGGLLLAQKALHKTSDTAFCLSCHSMSKPFEEYQGTVHFSNQKGIRAECADCHIPKSGMDY
LFAKLKASKDIYHEFVSGKIDSDDKFEAHRQEMAETVWKELKATDSATCRSCHSFDAMDIASQSESAQKMHTKAQKDGET
CIDCHKGIAHFPPEIKMDDNAAHELESQAATSVTNGAHIYPFKPSRIGELATVNPGTDLTVVDASGKQPIVLLQGYQMQG
SENTLYLAAGQRLALATLSEEGIKALTVNGEWQADEYGNQWRQASIQGVLTDPALADRKPLWQYAEKLDDTYCAGCHAPI
AADHYTVNAWPSIAKGMGARTSMSENELDILTRYFQYNAKDISEKQ

Specific function: Part of the anaerobic respiratory chain of trimethylamine-N-oxide reductase TorZ. Required for electron transfer to the TorZ terminal enzyme [H]

COG id: COG3005

COG function: function code C; Nitrate/TMAO reductases, membrane-bound tetraheme cytochrome c subunit

Gene ontology:

Cell location: Cell inner membrane; Single-pass type II membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TorC/TorY family [H]

Homologues:

Organism=Escherichia coli, GI1788181, Length=366, Percent_Identity=97.5409836065574, Blast_Score=728, Evalue=0.0,
Organism=Escherichia coli, GI1787230, Length=374, Percent_Identity=34.2245989304813, Blast_Score=216, Evalue=2e-57,
Organism=Escherichia coli, GI1788530, Length=180, Percent_Identity=43.8888888888889, Blast_Score=160, Evalue=8e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009154
- InterPro:   IPR011031
- InterPro:   IPR005126 [H]

Pfam domain/function: PF03264 Cytochrom_NNT [H]

EC number: NA

Molecular weight: Translated: 40263; Mature: 40263

Theoretical pI: Translated: 6.27; Mature: 6.27

Prosite motif: PS51008 MULTIHEME_CYTC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRGKKRIGLMFLLIAVVVGGGGLLLAQKALHKTSDTAFCLSCHSMSKPFEEYQGTVHFSN
CCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCEEEEEHHHHCCCHHHHCCEEEECC
QKGIRAECADCHIPKSGMDYLFAKLKASKDIYHEFVSGKIDSDDKFEAHRQEMAETVWKE
CCCCCCCHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHH
LKATDSATCRSCHSFDAMDIASQSESAQKMHTKAQKDGETCIDCHKGIAHFPPEIKMDDN
HHCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCHHHCCCCCEECCC
AAHELESQAATSVTNGAHIYPFKPSRIGELATVNPGTDLTVVDASGKQPIVLLQGYQMQG
HHHHHHHHHHHHHCCCCEEECCCCCCCCCEEEECCCCCEEEEECCCCCCEEEEECEEECC
SENTLYLAAGQRLALATLSEEGIKALTVNGEWQADEYGNQWRQASIQGVLTDPALADRKP
CCCEEEEECCCEEEEEEECCCCCEEEEECCCCCCHHHHHHHHHHHHCEEECCCCCCCCCH
LWQYAEKLDDTYCAGCHAPIAADHYTVNAWPSIAKGMGARTSMSENELDILTRYFQYNAK
HHHHHHHHCCHHHCCCCCCCCCCCEEECCCHHHHHCCCCCCCCCCCHHHHHHHHHHCCCC
DISEKQ
CCCCCC
>Mature Secondary Structure
MRGKKRIGLMFLLIAVVVGGGGLLLAQKALHKTSDTAFCLSCHSMSKPFEEYQGTVHFSN
CCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCEEEEEHHHHCCCHHHHCCEEEECC
QKGIRAECADCHIPKSGMDYLFAKLKASKDIYHEFVSGKIDSDDKFEAHRQEMAETVWKE
CCCCCCCHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHH
LKATDSATCRSCHSFDAMDIASQSESAQKMHTKAQKDGETCIDCHKGIAHFPPEIKMDDN
HHCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCHHHCCCCCEECCC
AAHELESQAATSVTNGAHIYPFKPSRIGELATVNPGTDLTVVDASGKQPIVLLQGYQMQG
HHHHHHHHHHHHHCCCCEEECCCCCCCCCEEEECCCCCEEEEECCCCCCEEEEECEEECC
SENTLYLAAGQRLALATLSEEGIKALTVNGEWQADEYGNQWRQASIQGVLTDPALADRKP
CCCEEEEECCCEEEEEEECCCCCEEEEECCCCCCHHHHHHHHHHHHCEEECCCCCCCCCH
LWQYAEKLDDTYCAGCHAPIAADHYTVNAWPSIAKGMGARTSMSENELDILTRYFQYNAK
HHHHHHHHCCHHHCCCCCCCCCCCEEECCCHHHHHCCCCCCCCCCCHHHHHHHHHHCCCC
DISEKQ
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: trimethylamine-N-oxide; NADH [C]

Specific reaction: trimethylamine-N-oxide + NADH = H2O + trimethylamine + NAD [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]