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The map label for this gene is ydjL [H]

Identifier: 218705274

GI number: 218705274

Start: 2093460

End: 2094536

Strand: Reverse

Name: ydjL [H]

Synonym: ECUMN_2065

Alternate gene names: 218705274

Gene position: 2094536-2093460 (Counterclockwise)

Preceding gene: 218705275

Following gene: 218705273

Centisome position: 40.26

GC content: 49.95

Gene sequence:

>1077_bases
ATGAAAGCACTGGCTCGGTTTGGCAAAGCCTTTGGCGGCTACAAGATGATCGATGTCCCACAACCCATTTGTGGCCCGGA
AGATGTAGTGATTGAAATTAAAGCCGCGGCAATCTGCGGCGCAGACATGAAGCACTACAATGTCGATAGCGGTTCTGATG
AGTTTAACTCTATCCGCGGCCATGAGTTCGCAGGTTGTATTGCGCAGGTTGGTGAAAAAGTCAAAGACTGGAAAGTGGGG
CAACGCGTCGTGTCAGATAACAGCGGTCACGTCTGCGGCGTTTGTCCGGCCTGTGAACAAGGTGATTTTCTGTGTTGTAC
AGATAAAGTAAACCTTGGTCTGGATAACAATACCTGGGGCGGTGGTTTTTCCAAATATTGTCTGGTTCCTGGTGAAATTC
TCAAAATTCATCGTCATGCGTTGTGGGAAATCCCTGATGGTGTTGATTATGAGGACGCAGCCGTACTTGACCCTATCTGT
AATGCCTACAAATCCATCGCGCAGCAATCGAAATTCCTCCCTGGTCAGGATGTAGTCGTCATCGGTACTGGCCCACTCGG
GCTGTTCTCCGTACAAATGGCGCGGATTATGGGGGCGGTAAATATCGTCGTCGTTGGTCTACAAGAAGATGTGGCGGTCC
GCTTCCCGGTTGCAAAAGAACTGGGTGCGACGGCAGTAGTAAATGGTTCTACCGAAGATGTGGTGGCGCGCTGCCAGCAA
ATATGTGGCAAAGACAATCTGGGGCTGGTGATTGAATGCTCCGGTGCCAATATCGCATTGAAACAAGCCATCGAAATGCT
CCGCCCGAACGGGGAAGTGGTACGCGTTGGAATGGGCTTCAAACCTCTTAATTTCTCGATTAATGACATTACCGCCTGGA
ACAAAAGCATCATTGGGCATATGGCCTATGACTCCACCTCATGGCGTAACGCTCTCCGGCTATTAGCCAGCGGCGCTATC
AAAGTCAAACCGATGATCACGCATCGTATCGGCCTGTCGCAATGGCGCGAAGGGTTTGATGCGATGGTCGATAAAACCGC
AATCAAAGTGATCATGACTTACGACTTTGATGAATAA

Upstream 100 bases:

>100_bases
AGATCATTATCTGTGTTTTACAGATTTAATGTTGACCTCAAAAATCATGTGGGTAAGTATGATGACCATCTAAGCAAAAC
AAAACACAGAGGATGACGCA

Downstream 100 bases:

>100_bases
CTACTCTCCTGAAGGAACGTTATCGCATGGAACAGATAACAAAACCGCATTGTGGTGCACGGCTGGATCGCTTACCGGAT
TGCCGCTGGCATTCGTCAAT

Product: putative oxidoreductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 358; Mature: 358

Protein sequence:

>358_residues
MKALARFGKAFGGYKMIDVPQPICGPEDVVIEIKAAAICGADMKHYNVDSGSDEFNSIRGHEFAGCIAQVGEKVKDWKVG
QRVVSDNSGHVCGVCPACEQGDFLCCTDKVNLGLDNNTWGGGFSKYCLVPGEILKIHRHALWEIPDGVDYEDAAVLDPIC
NAYKSIAQQSKFLPGQDVVVIGTGPLGLFSVQMARIMGAVNIVVVGLQEDVAVRFPVAKELGATAVVNGSTEDVVARCQQ
ICGKDNLGLVIECSGANIALKQAIEMLRPNGEVVRVGMGFKPLNFSINDITAWNKSIIGHMAYDSTSWRNALRLLASGAI
KVKPMITHRIGLSQWREGFDAMVDKTAIKVIMTYDFDE

Sequences:

>Translated_358_residues
MKALARFGKAFGGYKMIDVPQPICGPEDVVIEIKAAAICGADMKHYNVDSGSDEFNSIRGHEFAGCIAQVGEKVKDWKVG
QRVVSDNSGHVCGVCPACEQGDFLCCTDKVNLGLDNNTWGGGFSKYCLVPGEILKIHRHALWEIPDGVDYEDAAVLDPIC
NAYKSIAQQSKFLPGQDVVVIGTGPLGLFSVQMARIMGAVNIVVVGLQEDVAVRFPVAKELGATAVVNGSTEDVVARCQQ
ICGKDNLGLVIECSGANIALKQAIEMLRPNGEVVRVGMGFKPLNFSINDITAWNKSIIGHMAYDSTSWRNALRLLASGAI
KVKPMITHRIGLSQWREGFDAMVDKTAIKVIMTYDFDE
>Mature_358_residues
MKALARFGKAFGGYKMIDVPQPICGPEDVVIEIKAAAICGADMKHYNVDSGSDEFNSIRGHEFAGCIAQVGEKVKDWKVG
QRVVSDNSGHVCGVCPACEQGDFLCCTDKVNLGLDNNTWGGGFSKYCLVPGEILKIHRHALWEIPDGVDYEDAAVLDPIC
NAYKSIAQQSKFLPGQDVVVIGTGPLGLFSVQMARIMGAVNIVVVGLQEDVAVRFPVAKELGATAVVNGSTEDVVARCQQ
ICGKDNLGLVIECSGANIALKQAIEMLRPNGEVVRVGMGFKPLNFSINDITAWNKSIIGHMAYDSTSWRNALRLLASGAI
KVKPMITHRIGLSQWREGFDAMVDKTAIKVIMTYDFDE

Specific function: Unknown

COG id: COG1063

COG function: function code ER; Threonine dehydrogenase and related Zn-dependent dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the zinc-containing alcohol dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI156627571, Length=357, Percent_Identity=27.1708683473389, Blast_Score=130, Evalue=2e-30,
Organism=Escherichia coli, GI1788075, Length=358, Percent_Identity=98.8826815642458, Blast_Score=731, Evalue=0.0,
Organism=Escherichia coli, GI1790045, Length=366, Percent_Identity=30.6010928961749, Blast_Score=160, Evalue=1e-40,
Organism=Escherichia coli, GI1788073, Length=339, Percent_Identity=28.023598820059, Blast_Score=135, Evalue=3e-33,
Organism=Escherichia coli, GI226510992, Length=340, Percent_Identity=27.3529411764706, Blast_Score=117, Evalue=1e-27,
Organism=Escherichia coli, GI1787863, Length=340, Percent_Identity=27.6470588235294, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI1788407, Length=312, Percent_Identity=25.3205128205128, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI87082125, Length=364, Percent_Identity=27.1978021978022, Blast_Score=92, Evalue=5e-20,
Organism=Escherichia coli, GI1790718, Length=333, Percent_Identity=25.8258258258258, Blast_Score=82, Evalue=7e-17,
Organism=Escherichia coli, GI1786825, Length=381, Percent_Identity=25.4593175853018, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI87081918, Length=305, Percent_Identity=25.9016393442623, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI1786552, Length=356, Percent_Identity=24.7191011235955, Blast_Score=62, Evalue=5e-11,
Organism=Caenorhabditis elegans, GI17562876, Length=345, Percent_Identity=25.7971014492754, Blast_Score=118, Evalue=4e-27,
Organism=Caenorhabditis elegans, GI17562878, Length=335, Percent_Identity=25.0746268656716, Blast_Score=103, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI71988145, Length=276, Percent_Identity=27.1739130434783, Blast_Score=72, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI17562584, Length=300, Percent_Identity=29, Blast_Score=69, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6323099, Length=340, Percent_Identity=30, Blast_Score=144, Evalue=3e-35,
Organism=Saccharomyces cerevisiae, GI6319955, Length=336, Percent_Identity=30.3571428571429, Blast_Score=140, Evalue=2e-34,
Organism=Saccharomyces cerevisiae, GI6322619, Length=339, Percent_Identity=30.3834808259587, Blast_Score=140, Evalue=3e-34,
Organism=Saccharomyces cerevisiae, GI6319257, Length=395, Percent_Identity=27.8481012658228, Blast_Score=114, Evalue=3e-26,
Organism=Saccharomyces cerevisiae, GI6319258, Length=391, Percent_Identity=25.5754475703325, Blast_Score=106, Evalue=5e-24,
Organism=Saccharomyces cerevisiae, GI6319621, Length=334, Percent_Identity=29.0419161676647, Blast_Score=88, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6324486, Length=342, Percent_Identity=25.4385964912281, Blast_Score=74, Evalue=4e-14,
Organism=Saccharomyces cerevisiae, GI6323729, Length=338, Percent_Identity=27.810650887574, Blast_Score=74, Evalue=4e-14,
Organism=Saccharomyces cerevisiae, GI6323961, Length=342, Percent_Identity=26.0233918128655, Blast_Score=74, Evalue=4e-14,
Organism=Drosophila melanogaster, GI17737897, Length=351, Percent_Identity=31.3390313390313, Blast_Score=122, Evalue=3e-28,
Organism=Drosophila melanogaster, GI17137530, Length=335, Percent_Identity=29.8507462686567, Blast_Score=113, Evalue=2e-25,
Organism=Drosophila melanogaster, GI45551930, Length=330, Percent_Identity=24.2424242424242, Blast_Score=68, Evalue=8e-12,
Organism=Drosophila melanogaster, GI45550770, Length=330, Percent_Identity=24.2424242424242, Blast_Score=68, Evalue=8e-12,
Organism=Drosophila melanogaster, GI221457811, Length=330, Percent_Identity=24.2424242424242, Blast_Score=68, Evalue=9e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013149
- InterPro:   IPR013154
- InterPro:   IPR002085
- InterPro:   IPR002328
- InterPro:   IPR011032
- InterPro:   IPR016040 [H]

Pfam domain/function: PF08240 ADH_N; PF00107 ADH_zinc_N [H]

EC number: NA

Molecular weight: Translated: 38699; Mature: 38699

Theoretical pI: Translated: 5.92; Mature: 5.92

Prosite motif: PS00059 ADH_ZINC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.6 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
6.7 %Cys+Met (Translated Protein)
3.6 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
6.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKALARFGKAFGGYKMIDVPQPICGPEDVVIEIKAAAICGADMKHYNVDSGSDEFNSIRG
CCHHHHHHHHHCCEEEEECCCCCCCCCCEEEEEEEHEEECCCCCCCCCCCCCCHHHHCCC
HEFAGCIAQVGEKVKDWKVGQRVVSDNSGHVCGVCPACEQGDFLCCTDKVNLGLDNNTWG
CHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCEEEEECCEEECCCCCCCC
GGFSKYCLVPGEILKIHRHALWEIPDGVDYEDAAVLDPICNAYKSIAQQSKFLPGQDVVV
CCCCCEEECCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEE
IGTGPLGLFSVQMARIMGAVNIVVVGLQEDVAVRFPVAKELGATAVVNGSTEDVVARCQQ
EECCCHHHHHHHHHHHHCEEEEEEEECCCCCEEECCCHHHCCCEEEECCCHHHHHHHHHH
ICGKDNLGLVIECSGANIALKQAIEMLRPNGEVVRVGMGFKPLNFSINDITAWNKSIIGH
HHCCCCCCEEEEECCCCCHHHHHHHHHCCCCCEEEEECCEEECCCEECCCCHHHHHHHEE
MAYDSTSWRNALRLLASGAIKVKPMITHRIGLSQWREGFDAMVDKTAIKVIMTYDFDE
ECCCCHHHHHHHHHHHCCCEEEEEHHHHHCCHHHHHHHHHHHHHHCEEEEEEEECCCC
>Mature Secondary Structure
MKALARFGKAFGGYKMIDVPQPICGPEDVVIEIKAAAICGADMKHYNVDSGSDEFNSIRG
CCHHHHHHHHHCCEEEEECCCCCCCCCCEEEEEEEHEEECCCCCCCCCCCCCCHHHHCCC
HEFAGCIAQVGEKVKDWKVGQRVVSDNSGHVCGVCPACEQGDFLCCTDKVNLGLDNNTWG
CHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCEEEEECCEEECCCCCCCC
GGFSKYCLVPGEILKIHRHALWEIPDGVDYEDAAVLDPICNAYKSIAQQSKFLPGQDVVV
CCCCCEEECCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEE
IGTGPLGLFSVQMARIMGAVNIVVVGLQEDVAVRFPVAKELGATAVVNGSTEDVVARCQQ
EECCCHHHHHHHHHHHHCEEEEEEEECCCCCEEECCCHHHCCCEEEECCCHHHHHHHHHH
ICGKDNLGLVIECSGANIALKQAIEMLRPNGEVVRVGMGFKPLNFSINDITAWNKSIIGH
HHCCCCCCEEEEECCCCCHHHHHHHHHCCCCCEEEEECCEEECCCEECCCCHHHHHHHEE
MAYDSTSWRNALRLLASGAIKVKPMITHRIGLSQWREGFDAMVDKTAIKVIMTYDFDE
ECCCCHHHHHHHHHHHCCCEEEEEHHHHHCCHHHHHHHHHHHHHHCEEEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]