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The map label for this gene is ydfI [H]

Identifier: 218705025

GI number: 218705025

Start: 1838495

End: 1839955

Strand: Reverse

Name: ydfI [H]

Synonym: ECUMN_1809

Alternate gene names: 218705025

Gene position: 1839955-1838495 (Counterclockwise)

Preceding gene: 218705026

Following gene: 218705021

Centisome position: 35.37

GC content: 49.62

Gene sequence:

>1461_bases
ATGGGAAATAATTTGTTATCAGCAAAAGCGACGCTCCCTGTTTATGATCGTAATAACTTGGCCCCAAGAATTGTTCATTT
AGGCTTTGGCGCATTTCACCGTGCGCATCAGGGCGTGTATGCCGATATTCTTGCTACAAAACATTTCAGTGATTGGGGAT
ATTATGAGGTCAATTTAATCGGCGGCGAACAGCAAATTGCCGATTTACAACAGCAAGATAATCTTTATACCGTTGCGGAA
ATGTCGGCCGATGCGTGGACGGCTCGCGTCGTTGGCGTCGTTAAAAAAGCCTTGCACGTACAGATAGATGGCTTAGAAAC
CGTGTTGGCTGCGATGTGTGAACCGCAAATCGCGATTGTCTCTCTGACAATCACCGAAAAAGGGTATTTCCACTCTCCGG
CGACCGGACAGTTAATGCTCGATCACCCGATGGTCGCTGCCGACGTGCAAAATCCCCACCAGCCGAAAACTGCAACAGGG
GTGATTGTTGAGGCGCTGGCTCGCCGTAAAGCGGCAGGACTTCCCGCATTTACCGTCATGTCATGTGACAACATGCCAGA
AAACGGTCATGTTATGCGTGACGTTGTCACTTCCTACGCGCAAGCTGTTGATGTAAAACTGGCACAATGGATCGAAGTAA
ACGTGACTTTCCCATCAACAATGGTGGACCGTATTGTGCCCGCAGTGACAGAGGATACGCTGGCGAAAATCGAACAACTT
ACCGGTGTGCGCGATCCTGCTGGCGTTGCCTGTGAACCTTTCCGCCAGTGGGTAATAGAAGATAACTTTGTTGCCGGACG
TCCGGAATGGGAAAAAGCGGGAGCCGAACTGGTTAGTGATGTGCTGCCTTATGAAGAGATGAAGTTGCGCATGCTCAACG
GCAGTCATTCATTCCTGGCGTATCTGGGGTATCTTGCCGGATATCAGCACATTAATGACTGCATGGAAGATGAACATTAT
CGTCATGCAGCGTATGCCTTGATGTTGCAGGAACAAGCGCCGACGTTGAAAGTGCAGGGCGTTGATTTGCAAGATTACGC
TAACCGATTAATTGAACGCTATAGCAACCCGGCGCTACGTCATCGAACCTGGCAGATTGCGATGGATGGCAGCCAGAAAT
TGCCACAGCGGATGTTGGATTCTGTTCGCTGGCATCTGGCGCATGACAGCAAGTTCGATCTGCTGGCGCTGGGCGTCGCA
GGTTGGATGCGTTATGTCGGTGGTGTTGATGAACAGGGAAATCCGATAGAAATCAGTGACCCACTGTTACCTGTTATTCA
GAAGGCTGTACAAAGTAGTGCCGAAGGGAAAGCGCGCGTCCAGTCATTGCTGGCGATTAAGGCGATCTTTGGTGATGATT
TGCCAGACAATAGCTTGTTTACTGCAAAAGTGACGGAAGCGTACTTGTCTTTATTAGCGCATGGTGCGAAAGCGACCGTT
GCGAAATATTCCGTGAAGTAA

Upstream 100 bases:

>100_bases
GAAGTCTGTAAATCCTCCATCCCTTCTCTTACCGGAGAGGGGATTTTTTATTCATATAACAAAACATGCAGCTTGCCATA
TTTAAATTTAAGGAATTGTT

Downstream 100 bases:

>100_bases
AAAAAGCAATGCCGCCATCGGCGGCATTCACACGCTTAGTTCATGCCGAGACGAATCAGGTCCAGAGGTGCCAGTTTCTC
TTCACAACCTTCAACAACTA

Product: putative mannonate dehydrogenase

Products: D-fructuronate; NADH [C]

Alternate protein names: NA

Number of amino acids: Translated: 486; Mature: 485

Protein sequence:

>486_residues
MGNNLLSAKATLPVYDRNNLAPRIVHLGFGAFHRAHQGVYADILATKHFSDWGYYEVNLIGGEQQIADLQQQDNLYTVAE
MSADAWTARVVGVVKKALHVQIDGLETVLAAMCEPQIAIVSLTITEKGYFHSPATGQLMLDHPMVAADVQNPHQPKTATG
VIVEALARRKAAGLPAFTVMSCDNMPENGHVMRDVVTSYAQAVDVKLAQWIEVNVTFPSTMVDRIVPAVTEDTLAKIEQL
TGVRDPAGVACEPFRQWVIEDNFVAGRPEWEKAGAELVSDVLPYEEMKLRMLNGSHSFLAYLGYLAGYQHINDCMEDEHY
RHAAYALMLQEQAPTLKVQGVDLQDYANRLIERYSNPALRHRTWQIAMDGSQKLPQRMLDSVRWHLAHDSKFDLLALGVA
GWMRYVGGVDEQGNPIEISDPLLPVIQKAVQSSAEGKARVQSLLAIKAIFGDDLPDNSLFTAKVTEAYLSLLAHGAKATV
AKYSVK

Sequences:

>Translated_486_residues
MGNNLLSAKATLPVYDRNNLAPRIVHLGFGAFHRAHQGVYADILATKHFSDWGYYEVNLIGGEQQIADLQQQDNLYTVAE
MSADAWTARVVGVVKKALHVQIDGLETVLAAMCEPQIAIVSLTITEKGYFHSPATGQLMLDHPMVAADVQNPHQPKTATG
VIVEALARRKAAGLPAFTVMSCDNMPENGHVMRDVVTSYAQAVDVKLAQWIEVNVTFPSTMVDRIVPAVTEDTLAKIEQL
TGVRDPAGVACEPFRQWVIEDNFVAGRPEWEKAGAELVSDVLPYEEMKLRMLNGSHSFLAYLGYLAGYQHINDCMEDEHY
RHAAYALMLQEQAPTLKVQGVDLQDYANRLIERYSNPALRHRTWQIAMDGSQKLPQRMLDSVRWHLAHDSKFDLLALGVA
GWMRYVGGVDEQGNPIEISDPLLPVIQKAVQSSAEGKARVQSLLAIKAIFGDDLPDNSLFTAKVTEAYLSLLAHGAKATV
AKYSVK
>Mature_485_residues
GNNLLSAKATLPVYDRNNLAPRIVHLGFGAFHRAHQGVYADILATKHFSDWGYYEVNLIGGEQQIADLQQQDNLYTVAEM
SADAWTARVVGVVKKALHVQIDGLETVLAAMCEPQIAIVSLTITEKGYFHSPATGQLMLDHPMVAADVQNPHQPKTATGV
IVEALARRKAAGLPAFTVMSCDNMPENGHVMRDVVTSYAQAVDVKLAQWIEVNVTFPSTMVDRIVPAVTEDTLAKIEQLT
GVRDPAGVACEPFRQWVIEDNFVAGRPEWEKAGAELVSDVLPYEEMKLRMLNGSHSFLAYLGYLAGYQHINDCMEDEHYR
HAAYALMLQEQAPTLKVQGVDLQDYANRLIERYSNPALRHRTWQIAMDGSQKLPQRMLDSVRWHLAHDSKFDLLALGVAG
WMRYVGGVDEQGNPIEISDPLLPVIQKAVQSSAEGKARVQSLLAIKAIFGDDLPDNSLFTAKVTEAYLSLLAHGAKATVA
KYSVK

Specific function: Unknown

COG id: COG0246

COG function: function code G; Mannitol-1-phosphate/altronate dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mannitol dehydrogenase family. UxuB subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787823, Length=486, Percent_Identity=98.1481481481482, Blast_Score=990, Evalue=0.0,
Organism=Escherichia coli, GI1790779, Length=472, Percent_Identity=58.6864406779661, Blast_Score=557, Evalue=1e-160,
Organism=Escherichia coli, GI1788497, Length=464, Percent_Identity=54.9568965517241, Blast_Score=550, Evalue=1e-158,
Organism=Escherichia coli, GI48994885, Length=367, Percent_Identity=26.9754768392371, Blast_Score=109, Evalue=3e-25,
Organism=Escherichia coli, GI1790028, Length=260, Percent_Identity=27.6923076923077, Blast_Score=92, Evalue=7e-20,
Organism=Saccharomyces cerevisiae, GI6324401, Length=488, Percent_Identity=39.9590163934426, Blast_Score=359, Evalue=1e-100,
Organism=Saccharomyces cerevisiae, GI6320765, Length=488, Percent_Identity=39.9590163934426, Blast_Score=359, Evalue=1e-100,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008927
- InterPro:   IPR013328
- InterPro:   IPR000669
- InterPro:   IPR013118
- InterPro:   IPR023027
- InterPro:   IPR013131
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01232 Mannitol_dh; PF08125 Mannitol_dh_C [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 53672; Mature: 53541

Theoretical pI: Translated: 5.94; Mature: 5.94

Prosite motif: PS00974 MANNITOL_DHGENASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGNNLLSAKATLPVYDRNNLAPRIVHLGFGAFHRAHQGVYADILATKHFSDWGYYEVNLI
CCCCEEECCCEEEEECCCCCCCEEEEECHHHHHHHHCCHHHHHHHHCCCCCCCEEEEEEE
GGEQQIADLQQQDNLYTVAEMSADAWTARVVGVVKKALHVQIDGLETVLAAMCEPQIAIV
CCHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHHHHEEECCHHHHHHHHCCCCEEEE
SLTITEKGYFHSPATGQLMLDHPMVAADVQNPHQPKTATGVIVEALARRKAAGLPAFTVM
EEEEECCCCCCCCCCCCEEECCCEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEE
SCDNMPENGHVMRDVVTSYAQAVDVKLAQWIEVNVTFPSTMVDRIVPAVTEDTLAKIEQL
ECCCCCCCCCHHHHHHHHHHHHHHHEEEEEEEEEEECCHHHHHHHHHHCCHHHHHHHHHH
TGVRDPAGVACEPFRQWVIEDNFVAGRPEWEKAGAELVSDVLPYEEMKLRMLNGSHSFLA
HCCCCCCCCCHHHHHHHHHCCCEECCCCCHHHHHHHHHHHHCCHHHHHEEEECCCHHHHH
YLGYLAGYQHINDCMEDEHYRHAAYALMLQEQAPTLKVQGVDLQDYANRLIERYSNPALR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHCCCCHH
HRTWQIAMDGSQKLPQRMLDSVRWHLAHDSKFDLLALGVAGWMRYVGGVDEQGNPIEISD
CEEEEEEECCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEECC
PLLPVIQKAVQSSAEGKARVQSLLAIKAIFGDDLPDNSLFTAKVTEAYLSLLAHGAKATV
CHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEHHHHHHHHHHHHCCCHHHH
AKYSVK
EEECCC
>Mature Secondary Structure 
GNNLLSAKATLPVYDRNNLAPRIVHLGFGAFHRAHQGVYADILATKHFSDWGYYEVNLI
CCCEEECCCEEEEECCCCCCCEEEEECHHHHHHHHCCHHHHHHHHCCCCCCCEEEEEEE
GGEQQIADLQQQDNLYTVAEMSADAWTARVVGVVKKALHVQIDGLETVLAAMCEPQIAIV
CCHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHHHHEEECCHHHHHHHHCCCCEEEE
SLTITEKGYFHSPATGQLMLDHPMVAADVQNPHQPKTATGVIVEALARRKAAGLPAFTVM
EEEEECCCCCCCCCCCCEEECCCEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEE
SCDNMPENGHVMRDVVTSYAQAVDVKLAQWIEVNVTFPSTMVDRIVPAVTEDTLAKIEQL
ECCCCCCCCCHHHHHHHHHHHHHHHEEEEEEEEEEECCHHHHHHHHHHCCHHHHHHHHHH
TGVRDPAGVACEPFRQWVIEDNFVAGRPEWEKAGAELVSDVLPYEEMKLRMLNGSHSFLA
HCCCCCCCCCHHHHHHHHHCCCEECCCCCHHHHHHHHHHHHCCHHHHHEEEECCCHHHHH
YLGYLAGYQHINDCMEDEHYRHAAYALMLQEQAPTLKVQGVDLQDYANRLIERYSNPALR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHCCCCHH
HRTWQIAMDGSQKLPQRMLDSVRWHLAHDSKFDLLALGVAGWMRYVGGVDEQGNPIEISD
CEEEEEEECCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEECC
PLLPVIQKAVQSSAEGKARVQSLLAIKAIFGDDLPDNSLFTAKVTEAYLSLLAHGAKATV
CHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEHHHHHHHHHHHHCCCHHHH
AKYSVK
EEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: D-mannonate; NAD(+) [C]

Specific reaction: D-mannonate + NAD(+) = D-fructuronate + NADH [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]