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The map label for this gene is narY [H]

Identifier: 218704939

GI number: 218704939

Start: 1732923

End: 1734467

Strand: Reverse

Name: narY [H]

Synonym: ECUMN_1720

Alternate gene names: 218704939

Gene position: 1734467-1732923 (Counterclockwise)

Preceding gene: 218704940

Following gene: 218704938

Centisome position: 33.34

GC content: 53.79

Gene sequence:

>1545_bases
ATGAAAATACGTTCGCAAGTCGGCATGGTGCTTAACCTCGATAAATGTATCGGCTGCCATACCTGTTCGGTGACCTGTAA
AAACGTCTGGACCGGACGCGAAGGCATGGAGTACGCATGGTTTAACAACGTCGAAACCAAACCGGGCATTGGTTATCCGA
AAAACTGGGAAGATCAGGAAGAGTGGCAAGGCGGCTGGGTGCGTGATGTGAATGGCAAGATCCGCCCGCGTCTGGGCAGC
AAGATGGGCGTAATAACCAAAATCTTCGCTAACCCGGTGGTGCCGCAGATTGATGATTACTACGAACCTTTCACTTTCGA
CTACGAACATTTGCATAGCGCACCGGAAGGCAAACATATCCCTACTGCTCGTCCGCGTTCACTGATTGACGGCAAACGGA
TGGACAAAGTGATCTGGGGGCCAAACTGGGAAGAATTGTTGGGCGGCGAATTCGAAAAACGCGCCCGTGACCGCAACTTC
GAGGCCATGCAAAAGGAGATGTACGGGCAGTTTGAAAACACCTTCATGATGTACCTGCCGCGCTTGTGCGAACACTGCCT
CAATCCCAGTTGCGTGGCGACCTGCCCAAGCGGCGCTATCTACAAACGCGAAGAAGACGGCATTGTGCTGATCGATCAGG
ATAAATGCCGTGGCTGGCGTTTGTGCATTAGCGGTTGTCCGTACAAAAAAATCTACTTCAACTGGAAAAGCGGCAAGTCA
GAAAAATGTATCTTCTGTTACCCACGAATTGAGTCAGGCCAACCCACCGTGTGCTCAGAAACCTGCGTGGGTCGCATCCG
GTATCTGGGCGTGCTGCTTTACGACGCTGACCGCATCGAGGAAGCGGCGAGCACCGAGCGTGAAGTTGACCTCTATGAAC
GCCAGTGCGAAGTATTCCTCGATCCACACGATCCCTCAGTGATCGAGGAAGCCCTGAAACAAGGTATTCCACAAAACGTG
ATTGAAGCTGCCCAGCGTTCGCCAGTCTACAAAATGGCGATGGACTGGAAACTGGCGCTACCGCTGCACCCTGAATACCG
CACCCTGCCGATGGTCTGGTACGTTCCTCCGCTGTCACCGATTCAGTCCTACGCAGATGCGGGCGGTTTGCCGAAAAGCG
AAGGCGTGCTGCCAGCCATCGAGAGCCTGCGTATTCCTGTGCAATATCTCGCCAATATGTTAAGTGCCGGCGATACCGGT
CCGGTACTGCGGGCGCTAAAACGGATGATGGCGATGCGCCACTATATGCGTTCACAAACCGTGGAAGGCGTTACCGATAC
CCGCGCCATTGACGAAGTAGGCCTGAGCGTCGCCCAGGTCGAAGAGATGTATCGCTACCTCGCCATTGCCAACTATGAAG
ACCGTTTTGTCATTCCAACCAGTCATCGGGAAATGGCGGGCGATGCCTTCGCAGAACGCAATGGCTGCGGTTTTACCTTT
GGCGACGGTTGCCACGGCTCGGACAGCAAATTCAACCTGTTCAACAGTAGCCGTATCGATGCCATCAACATCACCGAAGT
ACGCGACAAAGCGGAGGGCGAATAA

Upstream 100 bases:

>100_bases
CCGTCGGCTCGAACCGCGACGAATTCATCATGATCCGCAAGATGAAGAACGTTAACTGGCTGGATGATGAAGGTCGCGAT
CAGGTACAGGAGGCGAAAAA

Downstream 100 bases:

>100_bases
TGCAGATCCTCAAAGTGATCGGCCTGTTGATGGAGTATCCGGACGAGCTGTTATGGGAATGTAAGGAGGACGCGCTGGCG
TTGATCCGCCGCGACGCACC

Product: nitrate reductase 2 subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 514; Mature: 514

Protein sequence:

>514_residues
MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTGREGMEYAWFNNVETKPGIGYPKNWEDQEEWQGGWVRDVNGKIRPRLGS
KMGVITKIFANPVVPQIDDYYEPFTFDYEHLHSAPEGKHIPTARPRSLIDGKRMDKVIWGPNWEELLGGEFEKRARDRNF
EAMQKEMYGQFENTFMMYLPRLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCRGWRLCISGCPYKKIYFNWKSGKS
EKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYDADRIEEAASTEREVDLYERQCEVFLDPHDPSVIEEALKQGIPQNV
IEAAQRSPVYKMAMDWKLALPLHPEYRTLPMVWYVPPLSPIQSYADAGGLPKSEGVLPAIESLRIPVQYLANMLSAGDTG
PVLRALKRMMAMRHYMRSQTVEGVTDTRAIDEVGLSVAQVEEMYRYLAIANYEDRFVIPTSHREMAGDAFAERNGCGFTF
GDGCHGSDSKFNLFNSSRIDAINITEVRDKAEGE

Sequences:

>Translated_514_residues
MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTGREGMEYAWFNNVETKPGIGYPKNWEDQEEWQGGWVRDVNGKIRPRLGS
KMGVITKIFANPVVPQIDDYYEPFTFDYEHLHSAPEGKHIPTARPRSLIDGKRMDKVIWGPNWEELLGGEFEKRARDRNF
EAMQKEMYGQFENTFMMYLPRLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCRGWRLCISGCPYKKIYFNWKSGKS
EKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYDADRIEEAASTEREVDLYERQCEVFLDPHDPSVIEEALKQGIPQNV
IEAAQRSPVYKMAMDWKLALPLHPEYRTLPMVWYVPPLSPIQSYADAGGLPKSEGVLPAIESLRIPVQYLANMLSAGDTG
PVLRALKRMMAMRHYMRSQTVEGVTDTRAIDEVGLSVAQVEEMYRYLAIANYEDRFVIPTSHREMAGDAFAERNGCGFTF
GDGCHGSDSKFNLFNSSRIDAINITEVRDKAEGE
>Mature_514_residues
MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTGREGMEYAWFNNVETKPGIGYPKNWEDQEEWQGGWVRDVNGKIRPRLGS
KMGVITKIFANPVVPQIDDYYEPFTFDYEHLHSAPEGKHIPTARPRSLIDGKRMDKVIWGPNWEELLGGEFEKRARDRNF
EAMQKEMYGQFENTFMMYLPRLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCRGWRLCISGCPYKKIYFNWKSGKS
EKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYDADRIEEAASTEREVDLYERQCEVFLDPHDPSVIEEALKQGIPQNV
IEAAQRSPVYKMAMDWKLALPLHPEYRTLPMVWYVPPLSPIQSYADAGGLPKSEGVLPAIESLRIPVQYLANMLSAGDTG
PVLRALKRMMAMRHYMRSQTVEGVTDTRAIDEVGLSVAQVEEMYRYLAIANYEDRFVIPTSHREMAGDAFAERNGCGFTF
GDGCHGSDSKFNLFNSSRIDAINITEVRDKAEGE

Specific function: This is a second nitrate reductase enzyme which can substitute for the NRA enzyme and allows E.coli to use nitrate as an electron acceptor during anaerobic growth. The beta chain is an electron transfer unit containing four cysteine clusters involved in t

COG id: COG1140

COG function: function code C; Nitrate reductase beta subunit

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1787740, Length=514, Percent_Identity=99.6108949416342, Blast_Score=1072, Evalue=0.0,
Organism=Escherichia coli, GI1787478, Length=506, Percent_Identity=79.0513833992095, Blast_Score=865, Evalue=0.0,
Organism=Escherichia coli, GI1787872, Length=101, Percent_Identity=41.5841584158416, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI1787122, Length=101, Percent_Identity=41.5841584158416, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI2367345, Length=111, Percent_Identity=29.7297297297297, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI1789370, Length=99, Percent_Identity=37.3737373737374, Blast_Score=67, Evalue=4e-12,
Organism=Escherichia coli, GI226510944, Length=90, Percent_Identity=35.5555555555556, Blast_Score=66, Evalue=4e-12,
Organism=Escherichia coli, GI1787749, Length=87, Percent_Identity=34.4827586206897, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1790326, Length=112, Percent_Identity=28.5714285714286, Blast_Score=62, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR006547 [H]

Pfam domain/function: NA

EC number: =1.7.99.4 [H]

Molecular weight: Translated: 58545; Mature: 58545

Theoretical pI: Translated: 5.72; Mature: 5.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.5 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
7.2 %Cys+Met (Translated Protein)
3.5 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
7.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTGREGMEYAWFNNVETKPGIGYPKNWEDQE
CCCHHHCCCEEEHHHHHCCCEEEEEHHHCCCCCCCCCEEECCCCCCCCCCCCCCCCCCHH
EWQGGWVRDVNGKIRPRLGSKMGVITKIFANPVVPQIDDYYEPFTFDYEHLHSAPEGKHI
HHCCCCEEECCCCCCCCCCCHHHHHHHHHCCCCCCCCHHCCCCCCCCHHHHCCCCCCCCC
PTARPRSLIDGKRMDKVIWGPNWEELLGGEFEKRARDRNFEAMQKEMYGQFENTFMMYLP
CCCCCHHHHCCHHHCCEEECCCHHHHHCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
RLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCRGWRLCISGCPYKKIYFNWKSGKS
HHHHHHCCCCEEEECCCCCEEEECCCCEEEEECCCCCCEEEEECCCCCEEEEEEECCCCC
EKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYDADRIEEAASTEREVDLYERQCEVFL
CCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHEECHHHHHHHHCCHHHHHHHHCCCCEEE
DPHDPSVIEEALKQGIPQNVIEAAQRSPVYKMAMDWKLALPLHPEYRTLPMVWYVPPLSP
CCCCHHHHHHHHHCCCCHHHHHHHHCCCCEEEEECEEEECCCCCCCCCCCEEEECCCCHH
IQSYADAGGLPKSEGVLPAIESLRIPVQYLANMLSAGDTGPVLRALKRMMAMRHYMRSQT
HHHHHHCCCCCCCCCCCHHHHHHCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
VEGVTDTRAIDEVGLSVAQVEEMYRYLAIANYEDRFVIPTSHREMAGDAFAERNGCGFTF
CCCCHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCEEECCCCHHHHHHHHHCCCCCCCCC
GDGCHGSDSKFNLFNSSRIDAINITEVRDKAEGE
CCCCCCCCCCEEEECCCCCCEEEHHHHHHHCCCC
>Mature Secondary Structure
MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTGREGMEYAWFNNVETKPGIGYPKNWEDQE
CCCHHHCCCEEEHHHHHCCCEEEEEHHHCCCCCCCCCEEECCCCCCCCCCCCCCCCCCHH
EWQGGWVRDVNGKIRPRLGSKMGVITKIFANPVVPQIDDYYEPFTFDYEHLHSAPEGKHI
HHCCCCEEECCCCCCCCCCCHHHHHHHHHCCCCCCCCHHCCCCCCCCHHHHCCCCCCCCC
PTARPRSLIDGKRMDKVIWGPNWEELLGGEFEKRARDRNFEAMQKEMYGQFENTFMMYLP
CCCCCHHHHCCHHHCCEEECCCHHHHHCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
RLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCRGWRLCISGCPYKKIYFNWKSGKS
HHHHHHCCCCEEEECCCCCEEEECCCCEEEEECCCCCCEEEEECCCCCEEEEEEECCCCC
EKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYDADRIEEAASTEREVDLYERQCEVFL
CCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHEECHHHHHHHHCCHHHHHHHHCCCCEEE
DPHDPSVIEEALKQGIPQNVIEAAQRSPVYKMAMDWKLALPLHPEYRTLPMVWYVPPLSP
CCCCHHHHHHHHHCCCCHHHHHHHHCCCCEEEEECEEEECCCCCCCCCCCEEEECCCCHH
IQSYADAGGLPKSEGVLPAIESLRIPVQYLANMLSAGDTGPVLRALKRMMAMRHYMRSQT
HHHHHHCCCCCCCCCCCHHHHHHCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
VEGVTDTRAIDEVGLSVAQVEEMYRYLAIANYEDRFVIPTSHREMAGDAFAERNGCGFTF
CCCCHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCEEECCCCHHHHHHHHHCCCCCCCCC
GDGCHGSDSKFNLFNSSRIDAINITEVRDKAEGE
CCCCCCCCCCEEEECCCCCCEEEHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2233673; 9097039; 9278503 [H]