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The map label for this gene is puuA

Identifier: 218704827

GI number: 218704827

Start: 1611347

End: 1612765

Strand: Reverse

Name: puuA

Synonym: ECUMN_1603

Alternate gene names: 218704827

Gene position: 1612765-1611347 (Counterclockwise)

Preceding gene: 218704833

Following gene: 218704826

Centisome position: 31.0

GC content: 53.28

Gene sequence:

>1419_bases
ATGGAAACCAATATCGTTGAAGTAGAGAACTTTGTTCAGCAGTCAGAAGAGAGGCGGGGTAGCGCCTTTACGCAGGAAGT
GAAACGCTACCTGGAGCGCTACCCGAATACGCAATATGTTGATGTTTTACTTACCGATTTGAATGGTTGCTTCCGTGGAA
AACGTATTCCTGTTTCAAGCCTGAAGAAGCTCGAGAAAGGGTGTTATTTCCCGGCCTCGGTGTTTGCAATGGATATTCTG
GGCAACGTAGTAGAAGAAGCGGGCCTGGGTCAGGAAATGGGCGAGCCGGATCGTACCTGTGTTCCTGTTCTCGGTTCCTT
AACCCCTTCTGCCGCCGATCCAGAGTTTATCGGTCAGATGCTCCTGACCATGGTCGATGAAGATGGCGCTCCCTTTGACG
TTGAGCCGCGGAACGTTCTTAACCGCCTGTGGCAGCAGCTGCGCCAGCGCGGATTGTTCCCGGTCGTAGCGGTAGAGCTG
GAGTTCTATTTACTGGATCGCCAGCGCGACGCTGAAGGATATCTGCAACCGCCCTGCGCGCCCGGCACCGATGACCGCAA
TACACAAAGCCAGGTTTACTCCGTTGATAACCTCAACCACTTCGCCGACGTGCTCAATGATATTGATGAGCTGGCGCAGT
TACAGCTGATTCCGGCAGATGGCGCGGTCGCTGAGGCCTCGCCAGGTCAGTTTGAAATCAATCTTTACCATACCGATAAC
GTGCTGGAAGCTTGCGATGATGCGCTGGCACTAAAACGCCTGGTGCGTCTGATGGCAGAAAAGCATAAGATGCACGCCAC
TTTTATGGCGAAGCCGTATGAAGAGCACGCGGGCAGCGGGATGCATATCCATATCAGTATGCAAAATAACCGTGGCGAGA
ACGTGCTTTCTGACGCGGAAGGCGAAGATTCGCCGCTGCTGAAAAAGATGCTTGCCGGGATGATTGACCTGATGCCGTCA
TCAATGGCGTTGCTGGCACCAAACGTGAACTCGTATCGCCGCTTCCAGCCAGGTATGTATGTACCGACGCAGGCGTCGTG
GGGCCATAACAACCGCACCGTCGCCCTGCGTATTCCGTGCGGCGATCGCCATAATCACCGCGTGGAATATCGCGTGGCGG
GTGCCGATGCCAACCCGTATCTGGTGATGGCGGCGATTTTTGCCGGTATTTTGCATGGCCTTGATAACGAACTGCCATTA
CAGGAAGAAGTCGAAGGTAACGGGCTGGAACAGGAAGGCTTACCCTTCCCGATTCGTCAGAGCGATGCCCTGGGTGAGTT
TATCGAGAATGATCACCTGCGCCGCTATTTAGGCGAACGGTTTTGCCATGTCTATCACGCCTGTAAAAATGATGAGCTGT
TGCAGTTTGAGCGCCTCATCACAGAAACAGAAATTGAGTGGATGTTGAAAAACGCGTAA

Upstream 100 bases:

>100_bases
TAACATTTGACAAACATTTAGTTTGCATACAGATTCGAATAGTGGTCATTATATTTTACGCTTTGATAACGAGCGGAAAA
CAAACCAAAGGCGAAGAATC

Downstream 100 bases:

>100_bases
TACCCCTTGCGGGGTTGTTTCATCGGCCTGAGCGGAGCCAGGCCTGACATTTGCTGGTAATCCAGACATTTTCCGGATGT
CGCGTTATGGAGCGCAGGCG

Product: gamma-Glu-putrescine synthase

Products: NA

Alternate protein names: Gamma-Glu-Put synthetase; Glutamate--putrescine ligase

Number of amino acids: Translated: 472; Mature: 472

Protein sequence:

>472_residues
METNIVEVENFVQQSEERRGSAFTQEVKRYLERYPNTQYVDVLLTDLNGCFRGKRIPVSSLKKLEKGCYFPASVFAMDIL
GNVVEEAGLGQEMGEPDRTCVPVLGSLTPSAADPEFIGQMLLTMVDEDGAPFDVEPRNVLNRLWQQLRQRGLFPVVAVEL
EFYLLDRQRDAEGYLQPPCAPGTDDRNTQSQVYSVDNLNHFADVLNDIDELAQLQLIPADGAVAEASPGQFEINLYHTDN
VLEACDDALALKRLVRLMAEKHKMHATFMAKPYEEHAGSGMHIHISMQNNRGENVLSDAEGEDSPLLKKMLAGMIDLMPS
SMALLAPNVNSYRRFQPGMYVPTQASWGHNNRTVALRIPCGDRHNHRVEYRVAGADANPYLVMAAIFAGILHGLDNELPL
QEEVEGNGLEQEGLPFPIRQSDALGEFIENDHLRRYLGERFCHVYHACKNDELLQFERLITETEIEWMLKNA

Sequences:

>Translated_472_residues
METNIVEVENFVQQSEERRGSAFTQEVKRYLERYPNTQYVDVLLTDLNGCFRGKRIPVSSLKKLEKGCYFPASVFAMDIL
GNVVEEAGLGQEMGEPDRTCVPVLGSLTPSAADPEFIGQMLLTMVDEDGAPFDVEPRNVLNRLWQQLRQRGLFPVVAVEL
EFYLLDRQRDAEGYLQPPCAPGTDDRNTQSQVYSVDNLNHFADVLNDIDELAQLQLIPADGAVAEASPGQFEINLYHTDN
VLEACDDALALKRLVRLMAEKHKMHATFMAKPYEEHAGSGMHIHISMQNNRGENVLSDAEGEDSPLLKKMLAGMIDLMPS
SMALLAPNVNSYRRFQPGMYVPTQASWGHNNRTVALRIPCGDRHNHRVEYRVAGADANPYLVMAAIFAGILHGLDNELPL
QEEVEGNGLEQEGLPFPIRQSDALGEFIENDHLRRYLGERFCHVYHACKNDELLQFERLITETEIEWMLKNA
>Mature_472_residues
METNIVEVENFVQQSEERRGSAFTQEVKRYLERYPNTQYVDVLLTDLNGCFRGKRIPVSSLKKLEKGCYFPASVFAMDIL
GNVVEEAGLGQEMGEPDRTCVPVLGSLTPSAADPEFIGQMLLTMVDEDGAPFDVEPRNVLNRLWQQLRQRGLFPVVAVEL
EFYLLDRQRDAEGYLQPPCAPGTDDRNTQSQVYSVDNLNHFADVLNDIDELAQLQLIPADGAVAEASPGQFEINLYHTDN
VLEACDDALALKRLVRLMAEKHKMHATFMAKPYEEHAGSGMHIHISMQNNRGENVLSDAEGEDSPLLKKMLAGMIDLMPS
SMALLAPNVNSYRRFQPGMYVPTQASWGHNNRTVALRIPCGDRHNHRVEYRVAGADANPYLVMAAIFAGILHGLDNELPL
QEEVEGNGLEQEGLPFPIRQSDALGEFIENDHLRRYLGERFCHVYHACKNDELLQFERLITETEIEWMLKNA

Specific function: Involved in the breakdown of putrescine via the biosynthesis of gamma-L-glutamylputrescine

COG id: COG0174

COG function: function code E; Glutamine synthetase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine synthetase family

Homologues:

Organism=Homo sapiens, GI221307485, Length=440, Percent_Identity=25.4545454545455, Blast_Score=96, Evalue=8e-20,
Organism=Escherichia coli, GI87081870, Length=472, Percent_Identity=100, Blast_Score=979, Evalue=0.0,
Organism=Escherichia coli, GI1790301, Length=401, Percent_Identity=26.1845386533666, Blast_Score=93, Evalue=5e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PUUA_ECOLI (P78061)

Other databases:

- EMBL:   U00096
- EMBL:   AP009048
- PIR:   D64878
- RefSeq:   AP_001923.1
- RefSeq:   NP_415813.4
- ProteinModelPortal:   P78061
- SMR:   P78061
- STRING:   P78061
- EnsemblBacteria:   EBESCT00000002830
- EnsemblBacteria:   EBESCT00000014992
- GeneID:   946202
- GenomeReviews:   AP009048_GR
- GenomeReviews:   U00096_GR
- KEGG:   ecj:JW5201
- KEGG:   eco:b1297
- EchoBASE:   EB3667
- EcoGene:   EG13908
- eggNOG:   COG0174
- GeneTree:   EBGT00050000010403
- HOGENOM:   HBG553755
- OMA:   WAYENRT
- ProtClustDB:   CLSK880118
- BioCyc:   EcoCyc:G6644-MONOMER
- BioCyc:   MetaCyc:G6644-MONOMER
- Genevestigator:   P78061
- InterPro:   IPR008147
- InterPro:   IPR014746
- InterPro:   IPR008146
- Gene3D:   G3DSA:3.30.590.10

Pfam domain/function: PF00120 Gln-synt_C; SSF54368 Gln_synt_beta

EC number: =6.3.1.11

Molecular weight: Translated: 53178; Mature: 53178

Theoretical pI: Translated: 4.57; Mature: 4.57

Prosite motif: PS00180 GLNA_1; PS00181 GLNA_ATP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
METNIVEVENFVQQSEERRGSAFTQEVKRYLERYPNTQYVDVLLTDLNGCFRGKRIPVSS
CCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHCCCCCCHHH
LKKLEKGCYFPASVFAMDILGNVVEEAGLGQEMGEPDRTCVPVLGSLTPSAADPEFIGQM
HHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHCCCCCCCCHHHHHHH
LLTMVDEDGAPFDVEPRNVLNRLWQQLRQRGLFPVVAVELEFYLLDRQRDAEGYLQPPCA
HHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEEEEEEECCCCCCCCCCCCCCC
PGTDDRNTQSQVYSVDNLNHFADVLNDIDELAQLQLIPADGAVAEASPGQFEINLYHTDN
CCCCCCCCHHHHEECCCHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCCEEEEEEEECCC
VLEACDDALALKRLVRLMAEKHKMHATFMAKPYEEHAGSGMHIHISMQNNRGENVLSDAE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCEEEEEEECCCCCCCCCCCCC
GEDSPLLKKMLAGMIDLMPSSMALLAPNVNSYRRFQPGMYVPTQASWGHNNRTVALRIPC
CCCCHHHHHHHHHHHHHCCCCCEEECCCCCHHHHCCCCCCCCCCCCCCCCCCEEEEEECC
GDRHNHRVEYRVAGADANPYLVMAAIFAGILHGLDNELPLQEEVEGNGLEQEGLPFPIRQ
CCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHCCCCCCCCCCCCCCCC
SDALGEFIENDHLRRYLGERFCHVYHACKNDELLQFERLITETEIEWMLKNA
CHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
METNIVEVENFVQQSEERRGSAFTQEVKRYLERYPNTQYVDVLLTDLNGCFRGKRIPVSS
CCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHCCCCCCHHH
LKKLEKGCYFPASVFAMDILGNVVEEAGLGQEMGEPDRTCVPVLGSLTPSAADPEFIGQM
HHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHCCCCCCCCHHHHHHH
LLTMVDEDGAPFDVEPRNVLNRLWQQLRQRGLFPVVAVELEFYLLDRQRDAEGYLQPPCA
HHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEEEEEEECCCCCCCCCCCCCCC
PGTDDRNTQSQVYSVDNLNHFADVLNDIDELAQLQLIPADGAVAEASPGQFEINLYHTDN
CCCCCCCCHHHHEECCCHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCCEEEEEEEECCC
VLEACDDALALKRLVRLMAEKHKMHATFMAKPYEEHAGSGMHIHISMQNNRGENVLSDAE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCEEEEEEECCCCCCCCCCCCC
GEDSPLLKKMLAGMIDLMPSSMALLAPNVNSYRRFQPGMYVPTQASWGHNNRTVALRIPC
CCCCHHHHHHHHHHHHHCCCCCEEECCCCCHHHHCCCCCCCCCCCCCCCCCCEEEEEECC
GDRHNHRVEYRVAGADANPYLVMAAIFAGILHGLDNELPLQEEVEGNGLEQEGLPFPIRQ
CCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHCCCCCCCCCCCCCCCC
SDALGEFIENDHLRRYLGERFCHVYHACKNDELLQFERLITETEIEWMLKNA
CHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503