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The map label for this gene is int [H]

Identifier: 218704536

GI number: 218704536

Start: 1350195

End: 1351313

Strand: Reverse

Name: int [H]

Synonym: ECUMN_1303

Alternate gene names: 218704536

Gene position: 1351313-1350195 (Counterclockwise)

Preceding gene: 218704537

Following gene: 218704535

Centisome position: 25.98

GC content: 45.31

Gene sequence:

>1119_bases
ATGTCACCGAGGCCACGAAAAAACAGCACTGACGTAGCCGGTCTTTACGAAAAGTTTGATCGCAGAACTGGCAGAGTTTA
CTACCAGTATAAAAATCCTGTGACTGGAAAATTTCACGGACTCGGAACAGACAAAGGTAAGGCAGAAAAAATCGCTTCCA
CAGCCAATCAGCGAATAGCTGCAGCAGAAGCTGAATATTTCATGCGCAAAATTGATGAAAGTCCGTCAGCAACAAAACGT
CGGGGTATCAGATTAAAGGCATGGGTTGATCGATATCTGAAAATACAGGACACGCGACTGAAAAATGGAGATATTGCAGC
TACAACTCACAAAGAAAAAACTCGAATGGCTGCATACCTGGTTTCCCGTCTGGGAAACCACCCATTGAAAGAACTGGAAG
TAAGAGACTTTGCATTAATACTGGATGAGTGGCTGGATAAAGACATGGTCAGCACAGCGAGAGTAAATCGTGGATTATGG
GTTGATATTTATAAAGAAGCACAGCATGCAGGGGAAGTTCCTCCTGGATGGAATCCTCCGGAGGCTACCCGTAAACCGAT
CCCTAAAGTAACCAGAGCCAGGCTCACCATGGAAGACTGGCAAAAAATTTACAATGCAACGCCTGAAAAACACTTTATCC
GTAACGCAATGCTTCTTGCGATTGTTACTGGTCAGCGCCGTGATGACATTTGCCACATGCGTTTTTCAGATGTGTGGAAC
GAACACTTGCATATCACCCAGGGAAAAACCGGAATGCGTCTGGCGTTACCGCTTACACTACGCTGTGATGCCATTGGGAT
AACGTTAAAAGAAGTTATTGATGGGTGCCGAGACAGAATATTAAGTCCATATCTAATCCATAGTCGGCACCAGAAACAAC
CGAAGCCGATGAGTAAAGACAACCTGAGCGACTACTTTGCCAAAGCACGGGATCTGGCTGGGATAATTCCACCAGCAGGA
AAAACTCCGCCAACATTTCATGAACAACGCTCTCTATCAGAACGGCTGTACCGTGCACAGGGTATCGATACAAAAACATT
ACTAGGACATAAAGTCCAGGCAACCACCGATCGCTATAACGATACTCGAGGTCAGGAATGGGTTAAGTTGGTTATTTGA

Upstream 100 bases:

>100_bases
CTGATAGATCGCCGAGCAGTATTTGTTGGGCCTGGTGAAACGGGAATTGCGCCGGAAATTCATACTGGCGATGATGATGC
ACTGAAGGAGATTTTAACTC

Downstream 100 bases:

>100_bases
CAAACACCATATGGAATATCCGTCACCATCTCAGGAAAAAAGTGTTGTAAAATGCGTGGCCTAGTTTTGGGGAAACGTTT
TGGAGAGATTTTGGAGAAAG

Product: Integrase from bacteriophage origin

Products: NA

Alternate protein names: Int(Lambda); Prophage e14 integrase [H]

Number of amino acids: Translated: 372; Mature: 371

Protein sequence:

>372_residues
MSPRPRKNSTDVAGLYEKFDRRTGRVYYQYKNPVTGKFHGLGTDKGKAEKIASTANQRIAAAEAEYFMRKIDESPSATKR
RGIRLKAWVDRYLKIQDTRLKNGDIAATTHKEKTRMAAYLVSRLGNHPLKELEVRDFALILDEWLDKDMVSTARVNRGLW
VDIYKEAQHAGEVPPGWNPPEATRKPIPKVTRARLTMEDWQKIYNATPEKHFIRNAMLLAIVTGQRRDDICHMRFSDVWN
EHLHITQGKTGMRLALPLTLRCDAIGITLKEVIDGCRDRILSPYLIHSRHQKQPKPMSKDNLSDYFAKARDLAGIIPPAG
KTPPTFHEQRSLSERLYRAQGIDTKTLLGHKVQATTDRYNDTRGQEWVKLVI

Sequences:

>Translated_372_residues
MSPRPRKNSTDVAGLYEKFDRRTGRVYYQYKNPVTGKFHGLGTDKGKAEKIASTANQRIAAAEAEYFMRKIDESPSATKR
RGIRLKAWVDRYLKIQDTRLKNGDIAATTHKEKTRMAAYLVSRLGNHPLKELEVRDFALILDEWLDKDMVSTARVNRGLW
VDIYKEAQHAGEVPPGWNPPEATRKPIPKVTRARLTMEDWQKIYNATPEKHFIRNAMLLAIVTGQRRDDICHMRFSDVWN
EHLHITQGKTGMRLALPLTLRCDAIGITLKEVIDGCRDRILSPYLIHSRHQKQPKPMSKDNLSDYFAKARDLAGIIPPAG
KTPPTFHEQRSLSERLYRAQGIDTKTLLGHKVQATTDRYNDTRGQEWVKLVI
>Mature_371_residues
SPRPRKNSTDVAGLYEKFDRRTGRVYYQYKNPVTGKFHGLGTDKGKAEKIASTANQRIAAAEAEYFMRKIDESPSATKRR
GIRLKAWVDRYLKIQDTRLKNGDIAATTHKEKTRMAAYLVSRLGNHPLKELEVRDFALILDEWLDKDMVSTARVNRGLWV
DIYKEAQHAGEVPPGWNPPEATRKPIPKVTRARLTMEDWQKIYNATPEKHFIRNAMLLAIVTGQRRDDICHMRFSDVWNE
HLHITQGKTGMRLALPLTLRCDAIGITLKEVIDGCRDRILSPYLIHSRHQKQPKPMSKDNLSDYFAKARDLAGIIPPAGK
TPPTFHEQRSLSERLYRAQGIDTKTLLGHKVQATTDRYNDTRGQEWVKLVI

Specific function: Integrase from the cryptic lambdoic prophage e14. Integrase is necessary for integration of the phage into the host genome by site-specific recombination. In conjunction with excisionase, integrase is also necessary for excision of the prophage from the h

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'phage' integrase family [H]

Homologues:

Organism=Escherichia coli, GI1787386, Length=377, Percent_Identity=47.2148541114058, Blast_Score=340, Evalue=1e-94,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016177
- InterPro:   IPR011010
- InterPro:   IPR013762
- InterPro:   IPR002104
- InterPro:   IPR015094
- InterPro:   IPR023109
- InterPro:   IPR013087 [H]

Pfam domain/function: PF09003 Phage_integ_N; PF00589 Phage_integrase [H]

EC number: NA

Molecular weight: Translated: 42708; Mature: 42576

Theoretical pI: Translated: 10.41; Mature: 10.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSPRPRKNSTDVAGLYEKFDRRTGRVYYQYKNPVTGKFHGLGTDKGKAEKIASTANQRIA
CCCCCCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCEEECCCCCCCHHHHHHHHHHHHHH
AAEAEYFMRKIDESPSATKRRGIRLKAWVDRYLKIQDTRLKNGDIAATTHKEKTRMAAYL
HHHHHHHHHHHCCCCCHHHHCCCCHHHHHHHHHHHHHCCCCCCCEEECCCHHHHHHHHHH
VSRLGNHPLKELEVRDFALILDEWLDKDMVSTARVNRGLWVDIYKEAQHAGEVPPGWNPP
HHHHCCCCHHHHHHHHHHHHHHHHHCHHHHHHHHHCCCCEEEEHHHHHHCCCCCCCCCCC
EATRKPIPKVTRARLTMEDWQKIYNATPEKHFIRNAMLLAIVTGQRRDDICHMRFSDVWN
HHHCCCCCHHHHHHCCHHHHHHHHCCCCHHHHHHHHHEEEEECCCCCCCHHHHHHHHHHC
EHLHITQGKTGMRLALPLTLRCDAIGITLKEVIDGCRDRILSPYLIHSRHQKQPKPMSKD
CCEEEECCCCCCEEEEEEEEEECHHCCCHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCC
NLSDYFAKARDLAGIIPPAGKTPPTFHEQRSLSERLYRAQGIDTKTLLGHKVQATTDRYN
CHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHCCCEEHHHHHCC
DTRGQEWVKLVI
CCCCHHHHEECC
>Mature Secondary Structure 
SPRPRKNSTDVAGLYEKFDRRTGRVYYQYKNPVTGKFHGLGTDKGKAEKIASTANQRIA
CCCCCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCEEECCCCCCCHHHHHHHHHHHHHH
AAEAEYFMRKIDESPSATKRRGIRLKAWVDRYLKIQDTRLKNGDIAATTHKEKTRMAAYL
HHHHHHHHHHHCCCCCHHHHCCCCHHHHHHHHHHHHHCCCCCCCEEECCCHHHHHHHHHH
VSRLGNHPLKELEVRDFALILDEWLDKDMVSTARVNRGLWVDIYKEAQHAGEVPPGWNPP
HHHHCCCCHHHHHHHHHHHHHHHHHCHHHHHHHHHCCCCEEEEHHHHHHCCCCCCCCCCC
EATRKPIPKVTRARLTMEDWQKIYNATPEKHFIRNAMLLAIVTGQRRDDICHMRFSDVWN
HHHCCCCCHHHHHHCCHHHHHHHHCCCCHHHHHHHHHEEEEECCCCCCCHHHHHHHHHHC
EHLHITQGKTGMRLALPLTLRCDAIGITLKEVIDGCRDRILSPYLIHSRHQKQPKPMSKD
CCEEEECCCCCCEEEEEEEEEECHHCCCHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCC
NLSDYFAKARDLAGIIPPAGKTPPTFHEQRSLSERLYRAQGIDTKTLLGHKVQATTDRYN
CHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHCCCEEHHHHHCC
DTRGQEWVKLVI
CCCCHHHHEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]