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The map label for this gene is rutB [H]

Identifier: 218704428

GI number: 218704428

Start: 1245068

End: 1245760

Strand: Reverse

Name: rutB [H]

Synonym: ECUMN_1194

Alternate gene names: 218704428

Gene position: 1245760-1245068 (Counterclockwise)

Preceding gene: 218704429

Following gene: 218704427

Centisome position: 23.95

GC content: 54.98

Gene sequence:

>693_bases
ATGACAATCTTAACCGCTCGACCGGAAGCCATTACCTTCGATCCGCAGCAAAGTGCGCTGATCGTGGTGGATATGCAAAA
CGCTTATGCCACGCCAGGCGGCTACTTAGATCTCGCCGGGTTTGATGTCTCAACCACTCGCCCGGTCATTGCCAACATTC
AAACCGCCGTGACCGCGGCACGAGCAGCAGGGATGCTGATCATCTGGTTTCAAAATGGCTGGGATGAACAGTATGTCGAG
GCTGGCGGCCCCGGCTCACCGAATTTTCATAAATCGAACGCCCTGAAAACCATGCGTAAGCAGCCGCAGCTGCAGGGGAA
ATTGCTGGCGAAAGGCTCCTGGGATTATCAACTGGTGGATGAACTGGTGCCGCAGCCTGGCGATATTGTGCTGCCGAAGC
CGCGCTACAGCGGTTTCTTCAATACGCCGCTGGACAGCATTTTGCGCAGCCGTGGAATACGCCATCTGGTTTTCACCGGT
ATCGCTACCAACGTCTGCGTCGAATCGACGCTACGCGATGGCTTTTTTCTGGAGTATTTCGGCGTGGTGCTGGAAGACGC
AACACACCAGGCGGGGCCGGAATTTGCACAGAAAGCCGCGTTATTCAATATCGAAACCTTTTTTGGCTGGGTCAGCGACG
TCGAAACGTTCTGCGACGCGCTTTCTCCCACGTCCTTTGCTCGTATCGCTTAA

Upstream 100 bases:

>100_bases
TCGATGATTTTCTGTCGGGAATCGAAACCTTCGGCGAGCGCATTCAACCACTGATGCAGTGCCGCGCCCATCTCCCTGCG
CTGACTCAGGAGGTGGCATG

Downstream 100 bases:

>100_bases
GGAGTTTAACGATGCCAAAATCCGTAATTATTCCCGCTGGCAGCAGCGCACCGCTGGCTCCCTTCGTTCCCGGCACGCTG
GCTGATGGCGTGGTGTATGT

Product: alternative pyrimidine degradation pathway protein

Products: NA

Alternate protein names: Ureidoacrylate amidohydrolase [H]

Number of amino acids: Translated: 230; Mature: 229

Protein sequence:

>230_residues
MTILTARPEAITFDPQQSALIVVDMQNAYATPGGYLDLAGFDVSTTRPVIANIQTAVTAARAAGMLIIWFQNGWDEQYVE
AGGPGSPNFHKSNALKTMRKQPQLQGKLLAKGSWDYQLVDELVPQPGDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFTG
IATNVCVESTLRDGFFLEYFGVVLEDATHQAGPEFAQKAALFNIETFFGWVSDVETFCDALSPTSFARIA

Sequences:

>Translated_230_residues
MTILTARPEAITFDPQQSALIVVDMQNAYATPGGYLDLAGFDVSTTRPVIANIQTAVTAARAAGMLIIWFQNGWDEQYVE
AGGPGSPNFHKSNALKTMRKQPQLQGKLLAKGSWDYQLVDELVPQPGDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFTG
IATNVCVESTLRDGFFLEYFGVVLEDATHQAGPEFAQKAALFNIETFFGWVSDVETFCDALSPTSFARIA
>Mature_229_residues
TILTARPEAITFDPQQSALIVVDMQNAYATPGGYLDLAGFDVSTTRPVIANIQTAVTAARAAGMLIIWFQNGWDEQYVEA
GGPGSPNFHKSNALKTMRKQPQLQGKLLAKGSWDYQLVDELVPQPGDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFTGI
ATNVCVESTLRDGFFLEYFGVVLEDATHQAGPEFAQKAALFNIETFFGWVSDVETFCDALSPTSFARIA

Specific function: In vivo, quickly hydrolyzes the ureidoacrylate peracid to avoid toxicity, but can also hydrolyzes ureidoacrylate that is formed spontaneously from ureidoacrylate peracid. One of the products of hydrolysis, carbamate, hydrolyzes spontaneously, thereby rele

COG id: COG1335

COG function: function code Q; Amidases related to nicotinamidase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isochorismatase family. RutB subfamily [H]

Homologues:

Organism=Escherichia coli, GI87081820, Length=230, Percent_Identity=98.695652173913, Blast_Score=470, Evalue=1e-134,
Organism=Escherichia coli, GI87081992, Length=211, Percent_Identity=28.436018957346, Blast_Score=65, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019916
- InterPro:   IPR000868 [H]

Pfam domain/function: PF00857 Isochorismatase [H]

EC number: NA

Molecular weight: Translated: 25241; Mature: 25110

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTILTARPEAITFDPQQSALIVVDMQNAYATPGGYLDLAGFDVSTTRPVIANIQTAVTAA
CEEEECCCCEEEECCCCCEEEEEECCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHH
RAAGMLIIWFQNGWDEQYVEAGGPGSPNFHKSNALKTMRKQPQLQGKLLAKGSWDYQLVD
HHCCEEEEEEECCCCHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCEEEECCCCCHHHHH
ELVPQPGDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFTGIATNVCVESTLRDGFFLEYF
HHCCCCCCEEECCCCCCCCCCCCHHHHHHHCCCCEEEHHHHHHHHHHHHHHCCCHHHHHH
GVVLEDATHQAGPEFAQKAALFNIETFFGWVSDVETFCDALSPTSFARIA
HHHHHHCCCCCCHHHHHHHHHEEHHHHHHHHHHHHHHHHHCCCCCHHHCC
>Mature Secondary Structure 
TILTARPEAITFDPQQSALIVVDMQNAYATPGGYLDLAGFDVSTTRPVIANIQTAVTAA
EEEECCCCEEEECCCCCEEEEEECCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHH
RAAGMLIIWFQNGWDEQYVEAGGPGSPNFHKSNALKTMRKQPQLQGKLLAKGSWDYQLVD
HHCCEEEEEEECCCCHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCEEEECCCCCHHHHH
ELVPQPGDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFTGIATNVCVESTLRDGFFLEYF
HHCCCCCCEEECCCCCCCCCCCCHHHHHHHCCCCEEEHHHHHHHHHHHHHHCCCHHHHHH
GVVLEDATHQAGPEFAQKAALFNIETFFGWVSDVETFCDALSPTSFARIA
HHHHHHCCCCCCHHHHHHHHHEEHHHHHHHHHHHHHHHHHCCCCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA