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The map label for this gene is citT

Identifier: 218703946

GI number: 218703946

Start: 758618

End: 760081

Strand: Reverse

Name: citT

Synonym: ECUMN_0705

Alternate gene names: 218703946

Gene position: 760081-758618 (Counterclockwise)

Preceding gene: 218703947

Following gene: 218703945

Centisome position: 14.61

GC content: 50.55

Gene sequence:

>1464_bases
ATGTCTTTAGCAAAAGATAATATATGGAAATTATTGGCGCCCCTGGTGGTGATGGGTGTCATGTTTCTTATCCCTGTACC
TGACGGTATGCCGCCGCAGGCGTGGCATTACTTTGCAGTGTTTGTGGCAATGATTGTCGGCATGATCCTCGAGCCAATTC
CGGCAACGGCGATCAGTTTTATTGCGGTTACTATTTGCGTTATTGGCAGTAATTACCTGCTCTTTGATGCCAAAGAATTA
GCTGACCCAGCGTTTAATGCGCAAAAACAGGCGCTGAAATGGGGTCTGGCTGGTTTTTCCAGCACCACGGTATGGCTGGT
ATTTGGCGCATTTATTTTTGCATTAGGGTATGAAGTTTCCGGGTTAGGTCGTCGCATTGCCCTTTTCCTGGTGAAATTCA
TGGGCAAACGCACGCTGACGTTGGGTTATGCGATTGTCATTATCGACATTCTGCTGGCACCGTTTACACCGTCCAACACC
GCGCGTACCGGGGGTACGGTTTTTCCGGTCATTAAAAACCTGCCGCCGCTGTTTAAATCATTCCCGAACGATCCGTCCGC
GCGTCGTATTGGCGGCTATTTGATGTGGATGATGGTCATTAGTACCAGTCTGAGTTCGTCCATGTTTGTCACCGGTGCGG
CACCAAACGTACTGGGTCTGGAGTTCGTCAGCAAAATTGCCGGTATCCAGATTAGCTGGTTGCAGTGGTTCCTCTGCTTC
CTGCCGGTTGGGGTTATCTTGCTTATCATTGCGCCGTGGCTTTCCTACGTGCTGTACAAACCGGAAATCACACACAGTGA
AGAAGTGGCAACCTGGGCGGGTGATGAACTAAAAACCATGGGTGCGCTGACACGCAGAGAGTGGACGCTGATTGGTCTTG
TATTGCTCAGCTTAGGTTTGTGGGTATTTGGCAGTGAAGTCATTAATGCTACTGCGGTAGGTCTGCTGGCAGTTTCGCTA
ATGCTGGCCCTGCACGTTGTGCCGTGGAAAGACATTACCCGCTATAACAGCGCATGGAACACACTGGTCAACCTGGCAAC
TCTGGTTGTGATGGCTAATGGCCTGACTCGTTCTGGTTTTATTGACTGGTTCGCCGGTACCATGAGTACGCACCTGGAAG
GATTCTCACCAAACGCAACGGTGATTGTACTGGTTCTGGTGTTCTACTTTGCGCACTACCTGTTTGCCAGCCTGTCTGCG
CACACCGCAACCATGCTGCCGGTTATTCTGGCCGTCGGTAAAGGTATTCCGGGCGTACCAATGGAACAACTGTGTATCCT
GCTGGTGCTGTCTATCGGTATCATGGGCTGTCTGACGCCGTATGCAACCGGTCCTGGGGTGATTATTTACGGCTGTGGCT
ATGTGAAATCAAAAGATTACTGGCGACTTGGTGCAATCTTCGGGGTGATTTACATCTCTATGCTGCTGTTGGTTGGCTGG
CCGATTCTCGCCATGTGGAACTAA

Upstream 100 bases:

>100_bases
GGCGGCAGTGCCGACCTGCTGATCCTTACTTGGTTTTTAGCACAGATTTAATTATTTAAGCACTTGATAAAATTGGAATA
TTAATTGTCGGAGAACCCGT

Downstream 100 bases:

>100_bases
TACGTTCGCCAAACGCTGAATAAATCATCACGCCCGCCAGGTAAGCCCACCTGGCGGGCTTTTTTATGATTTAATAGATA
GTATTCAGTGTATGTATGAG

Product: citrate:succinate antiporter

Products: NA

Alternate protein names: Citrate transporter; Citrate/succinate antiporter

Number of amino acids: Translated: 487; Mature: 486

Protein sequence:

>487_residues
MSLAKDNIWKLLAPLVVMGVMFLIPVPDGMPPQAWHYFAVFVAMIVGMILEPIPATAISFIAVTICVIGSNYLLFDAKEL
ADPAFNAQKQALKWGLAGFSSTTVWLVFGAFIFALGYEVSGLGRRIALFLVKFMGKRTLTLGYAIVIIDILLAPFTPSNT
ARTGGTVFPVIKNLPPLFKSFPNDPSARRIGGYLMWMMVISTSLSSSMFVTGAAPNVLGLEFVSKIAGIQISWLQWFLCF
LPVGVILLIIAPWLSYVLYKPEITHSEEVATWAGDELKTMGALTRREWTLIGLVLLSLGLWVFGSEVINATAVGLLAVSL
MLALHVVPWKDITRYNSAWNTLVNLATLVVMANGLTRSGFIDWFAGTMSTHLEGFSPNATVIVLVLVFYFAHYLFASLSA
HTATMLPVILAVGKGIPGVPMEQLCILLVLSIGIMGCLTPYATGPGVIIYGCGYVKSKDYWRLGAIFGVIYISMLLLVGW
PILAMWN

Sequences:

>Translated_487_residues
MSLAKDNIWKLLAPLVVMGVMFLIPVPDGMPPQAWHYFAVFVAMIVGMILEPIPATAISFIAVTICVIGSNYLLFDAKEL
ADPAFNAQKQALKWGLAGFSSTTVWLVFGAFIFALGYEVSGLGRRIALFLVKFMGKRTLTLGYAIVIIDILLAPFTPSNT
ARTGGTVFPVIKNLPPLFKSFPNDPSARRIGGYLMWMMVISTSLSSSMFVTGAAPNVLGLEFVSKIAGIQISWLQWFLCF
LPVGVILLIIAPWLSYVLYKPEITHSEEVATWAGDELKTMGALTRREWTLIGLVLLSLGLWVFGSEVINATAVGLLAVSL
MLALHVVPWKDITRYNSAWNTLVNLATLVVMANGLTRSGFIDWFAGTMSTHLEGFSPNATVIVLVLVFYFAHYLFASLSA
HTATMLPVILAVGKGIPGVPMEQLCILLVLSIGIMGCLTPYATGPGVIIYGCGYVKSKDYWRLGAIFGVIYISMLLLVGW
PILAMWN
>Mature_486_residues
SLAKDNIWKLLAPLVVMGVMFLIPVPDGMPPQAWHYFAVFVAMIVGMILEPIPATAISFIAVTICVIGSNYLLFDAKELA
DPAFNAQKQALKWGLAGFSSTTVWLVFGAFIFALGYEVSGLGRRIALFLVKFMGKRTLTLGYAIVIIDILLAPFTPSNTA
RTGGTVFPVIKNLPPLFKSFPNDPSARRIGGYLMWMMVISTSLSSSMFVTGAAPNVLGLEFVSKIAGIQISWLQWFLCFL
PVGVILLIIAPWLSYVLYKPEITHSEEVATWAGDELKTMGALTRREWTLIGLVLLSLGLWVFGSEVINATAVGLLAVSLM
LALHVVPWKDITRYNSAWNTLVNLATLVVMANGLTRSGFIDWFAGTMSTHLEGFSPNATVIVLVLVFYFAHYLFASLSAH
TATMLPVILAVGKGIPGVPMEQLCILLVLSIGIMGCLTPYATGPGVIIYGCGYVKSKDYWRLGAIFGVIYISMLLLVGWP
ILAMWN

Specific function: Responsible for the uptake of citrate in exchange to the efflux of succinate. Has a relatively broad specificity for C(4)- dicarboxylates and tricarboxylates

COG id: COG0471

COG function: function code P; Di- and tricarboxylate transporters

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the SLC13A transporter (TC 2.A.47) family. SODIT1 subfamily

Homologues:

Organism=Escherichia coli, GI1786829, Length=487, Percent_Identity=100, Blast_Score=967, Evalue=0.0,
Organism=Escherichia coli, GI1789444, Length=471, Percent_Identity=44.7983014861996, Blast_Score=438, Evalue=1e-124,
Organism=Escherichia coli, GI1786986, Length=493, Percent_Identity=33.4685598377282, Blast_Score=258, Evalue=6e-70,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CITT_ECO57 (P0AE75)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   C90710
- RefSeq:   NP_286339.1
- RefSeq:   NP_308678.1
- ProteinModelPortal:   P0AE75
- EnsemblBacteria:   EBESCT00000024140
- EnsemblBacteria:   EBESCT00000057715
- GeneID:   917010
- GeneID:   957636
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z0756
- KEGG:   ecs:ECs0651
- GeneTree:   EBGT00050000009673
- HOGENOM:   HBG321139
- OMA:   YSSAWNT
- ProtClustDB:   CLSK872279
- BioCyc:   ECOL83334:ECS0651-MONOMER
- InterPro:   IPR001898
- TIGRFAMs:   TIGR00785

Pfam domain/function: PF00939 Na_sulph_symp

EC number: NA

Molecular weight: Translated: 53093; Mature: 52962

Theoretical pI: Translated: 8.84; Mature: 8.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x2cb474b0)-; HASH(0x2cd72068)-; HASH(0x2aacc908)-; HASH(0x2a2ae848)-; HASH(0x2b176ac0)-; HASH(0x2b66ce90)-; HASH(0x2b1e8128)-; HASH(0x409a5b40)-; HASH(0x409b7048)-; HASH(0x2bc03a04)-; HASH(0x2bd19a2c)-; HASH(0x409ea1bc)-; HASH(0x2cf837e8)-; HASH(0x2bc06548)-;

Cys/Met content:

1.0 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLAKDNIWKLLAPLVVMGVMFLIPVPDGMPPQAWHYFAVFVAMIVGMILEPIPATAISF
CCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHH
IAVTICVIGSNYLLFDAKELADPAFNAQKQALKWGLAGFSSTTVWLVFGAFIFALGYEVS
HHHHHHHHCCCEEEEEHHHHCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCHH
GLGRRIALFLVKFMGKRTLTLGYAIVIIDILLAPFTPSNTARTGGTVFPVIKNLPPLFKS
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEHHHHHCCCHHHHC
FPNDPSARRIGGYLMWMMVISTSLSSSMFVTGAAPNVLGLEFVSKIAGIQISWLQWFLCF
CCCCCCHHHHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHCCCHHHHHHHHHH
LPVGVILLIIAPWLSYVLYKPEITHSEEVATWAGDELKTMGALTRREWTLIGLVLLSLGL
HHHHHHHHHHHHHHHHHEECCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHH
WVFGSEVINATAVGLLAVSLMLALHVVPWKDITRYNSAWNTLVNLATLVVMANGLTRSGF
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCH
IDWFAGTMSTHLEGFSPNATVIVLVLVFYFAHYLFASLSAHTATMLPVILAVGKGIPGVP
HHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
MEQLCILLVLSIGIMGCLTPYATGPGVIIYGCGYVKSKDYWRLGAIFGVIYISMLLLVGW
HHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCC
PILAMWN
CHHCCCH
>Mature Secondary Structure 
SLAKDNIWKLLAPLVVMGVMFLIPVPDGMPPQAWHYFAVFVAMIVGMILEPIPATAISF
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHH
IAVTICVIGSNYLLFDAKELADPAFNAQKQALKWGLAGFSSTTVWLVFGAFIFALGYEVS
HHHHHHHHCCCEEEEEHHHHCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCHH
GLGRRIALFLVKFMGKRTLTLGYAIVIIDILLAPFTPSNTARTGGTVFPVIKNLPPLFKS
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEHHHHHCCCHHHHC
FPNDPSARRIGGYLMWMMVISTSLSSSMFVTGAAPNVLGLEFVSKIAGIQISWLQWFLCF
CCCCCCHHHHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHCCCHHHHHHHHHH
LPVGVILLIIAPWLSYVLYKPEITHSEEVATWAGDELKTMGALTRREWTLIGLVLLSLGL
HHHHHHHHHHHHHHHHHEECCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHH
WVFGSEVINATAVGLLAVSLMLALHVVPWKDITRYNSAWNTLVNLATLVVMANGLTRSGF
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCH
IDWFAGTMSTHLEGFSPNATVIVLVLVFYFAHYLFASLSAHTATMLPVILAVGKGIPGVP
HHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
MEQLCILLVLSIGIMGCLTPYATGPGVIIYGCGYVKSKDYWRLGAIFGVIYISMLLLVGW
HHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCC
PILAMWN
CHHCCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796