Click here to switch to the map view.

The map label for this gene is entD [H]

Identifier: 218703917

GI number: 218703917

Start: 724585

End: 725355

Strand: Reverse

Name: entD [H]

Synonym: ECUMN_0675

Alternate gene names: 218703917

Gene position: 725355-724585 (Counterclockwise)

Preceding gene: 218703918

Following gene: 218703913

Centisome position: 13.94

GC content: 48.51

Gene sequence:

>771_bases
GTGCGCGTCGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAATCTTGCCAATTCAATATATTGCAGGA
CCATGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTTGCGTTTGTCATCAGTCTCGAATATAGTCGATATGAAAACTA
CGCATACCGCCCTCCCCTTTGCCGGACATACGCTGCATTTTGTTGAGTTCGATCCGGCGAGTTTTCGCGAGCAGGATTTA
CTCTGGCTGCCACACTACGCACAACTGCAACACGCTGGACGTAAACGTAAAACAGAGCATTTAGCTGGACGAATCGCTGC
TGTTTATGCCTTGCGGGAATATGGCTATAAATATGTGCCCGCTATCGGCGAGCTACGCCAACCTGTGTGGCCTGCGGAGG
TATACGGCAGTATTAGCCACTGCGGGACTACGGCATTAGCCGTGGTATCTCGTCAACCGATTGGCATTGATATCGAAGAG
ATTTTCTCTGTACAAACCGCAAGAGAATTGACAGACAACATTATTACACCAGCAGAACACGAGCGACTCGCAGAATGCGG
TTTAACCTTTTCTCTGGCGCTGACACTGGCATTTTCCGCCAAAGAGAGCGCATTTAAGGCAAGCAAGATACAGGCCGCTC
AAGGTTTTCTGGATTATCAGATAATTAGCTGGAATAAACAGCGGATCATCATTCGACTAGAGGATGAGCAGTTTGCTGTG
CACTGGCAGATAAAAGAAAAGATAGTCATAACGCTGTGCCAACACGATTAA

Upstream 100 bases:

>100_bases
TATCGCCGGTGCCGGGGCGTATACCTATAACGAGCCGGGACGTACGTGGTATATGAGCATTAATACTCACTTCTGATGCT
AACGTCAGATTGTTGACAAA

Downstream 100 bases:

>100_bases
TTGACAACTTCTGGTACGATTCGCCCGCAGCCATCACTGACCGCGGGCGAAAGTGTAAAGCAGGTGCCTTACCACCCTGA
CCTGACAACCGGATATGCGG

Product: phosphopantetheinyltransferase component of enterobactin synthase multienzyme complex

Products: NA

Alternate protein names: Enterobactin synthase component D; Enterochelin synthase D [H]

Number of amino acids: Translated: 256; Mature: 256

Protein sequence:

>256_residues
MRVVHAGCGVNALSGLQKSCQFNILQDHVGLISVAHQAVLRLSSVSNIVDMKTTHTALPFAGHTLHFVEFDPASFREQDL
LWLPHYAQLQHAGRKRKTEHLAGRIAAVYALREYGYKYVPAIGELRQPVWPAEVYGSISHCGTTALAVVSRQPIGIDIEE
IFSVQTARELTDNIITPAEHERLAECGLTFSLALTLAFSAKESAFKASKIQAAQGFLDYQIISWNKQRIIIRLEDEQFAV
HWQIKEKIVITLCQHD

Sequences:

>Translated_256_residues
MRVVHAGCGVNALSGLQKSCQFNILQDHVGLISVAHQAVLRLSSVSNIVDMKTTHTALPFAGHTLHFVEFDPASFREQDL
LWLPHYAQLQHAGRKRKTEHLAGRIAAVYALREYGYKYVPAIGELRQPVWPAEVYGSISHCGTTALAVVSRQPIGIDIEE
IFSVQTARELTDNIITPAEHERLAECGLTFSLALTLAFSAKESAFKASKIQAAQGFLDYQIISWNKQRIIIRLEDEQFAV
HWQIKEKIVITLCQHD
>Mature_256_residues
MRVVHAGCGVNALSGLQKSCQFNILQDHVGLISVAHQAVLRLSSVSNIVDMKTTHTALPFAGHTLHFVEFDPASFREQDL
LWLPHYAQLQHAGRKRKTEHLAGRIAAVYALREYGYKYVPAIGELRQPVWPAEVYGSISHCGTTALAVVSRQPIGIDIEE
IFSVQTARELTDNIITPAEHERLAECGLTFSLALTLAFSAKESAFKASKIQAAQGFLDYQIISWNKQRIIIRLEDEQFAV
HWQIKEKIVITLCQHD

Specific function: Catalyzes the transfer of the 4'-phosphopantetheine moiety from coenzyme A to apo-domains of both entB (an ArCP domain) and entF (a PCP domain). Plays an essential role in the assembly of the enterobactin [H]

COG id: COG2977

COG function: function code Q; Phosphopantetheinyl transferase component of siderophore synthetase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the P-Pant transferase superfamily. EntD family [H]

Homologues:

Organism=Escherichia coli, GI1786797, Length=209, Percent_Identity=91.866028708134, Blast_Score=401, Evalue=1e-113,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008278
- InterPro:   IPR003542 [H]

Pfam domain/function: PF01648 ACPS [H]

EC number: 2.7.8.-

Molecular weight: Translated: 28639; Mature: 28639

Theoretical pI: Translated: 7.40; Mature: 7.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVVHAGCGVNALSGLQKSCQFNILQDHVGLISVAHQAVLRLSSVSNIVDMKTTHTALPF
CEEEECCCCCHHHHHHHHHHCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
AGHTLHFVEFDPASFREQDLLWLPHYAQLQHAGRKRKTEHLAGRIAAVYALREYGYKYVP
CCCEEEEEEECCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECC
AIGELRQPVWPAEVYGSISHCGTTALAVVSRQPIGIDIEEIFSVQTARELTDNIITPAEH
CHHHHHCCCCCHHHHCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHH
ERLAECGLTFSLALTLAFSAKESAFKASKIQAAQGFLDYQIISWNKQRIIIRLEDEQFAV
HHHHHHCCCEEEEEEHHHHCHHHHHHHHHHHHHHCCEEEEEEEECCEEEEEEEECCEEEE
HWQIKEKIVITLCQHD
EEEEECEEEEEEECCC
>Mature Secondary Structure
MRVVHAGCGVNALSGLQKSCQFNILQDHVGLISVAHQAVLRLSSVSNIVDMKTTHTALPF
CEEEECCCCCHHHHHHHHHHCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
AGHTLHFVEFDPASFREQDLLWLPHYAQLQHAGRKRKTEHLAGRIAAVYALREYGYKYVP
CCCEEEEEEECCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECC
AIGELRQPVWPAEVYGSISHCGTTALAVVSRQPIGIDIEEIFSVQTARELTDNIITPAEH
CHHHHHCCCCCHHHHCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHH
ERLAECGLTFSLALTLAFSAKESAFKASKIQAAQGFLDYQIISWNKQRIIIRLEDEQFAV
HHHHHHCCCEEEEEEHHHHCHHHHHHHHHHHHHHCCEEEEEEEECCEEEEEEEECCEEEE
HWQIKEKIVITLCQHD
EEEEECEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]