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The map label for this gene is cusS [H]

Identifier: 218703902

GI number: 218703902

Start: 709432

End: 710892

Strand: Reverse

Name: cusS [H]

Synonym: ECUMN_0660

Alternate gene names: 218703902

Gene position: 710892-709432 (Counterclockwise)

Preceding gene: 218703903

Following gene: 218703901

Centisome position: 13.67

GC content: 51.88

Gene sequence:

>1461_bases
TTGAGGTGCCGGATGGTCAGTAAGCCATTTCAGCGCCCGTTTTCGCTGGCAACCCGCCTGACCTTTTTTATCAGCCTCGC
CACCATCGCGGCGTTTTTCGCCTTTGCATGGATAATGATCCACTCGGTAAAAGTGCATTTTGCCGAGCAGGATATTAATG
ATTTAAAAGAAATTAGCGCCACACTTGAACGGGTACTTAATCACCCTGACGAAACGCAAGCCCGACGCTTAATGACGCTG
GAAGATATTGTCAGTGGTTATTCCAATGTGTTGATCTCCCTGGCAGATAGCCACGGTAAAACGGTATATCACTCCCCCGG
TGCGCCCGATATCCGCGAGTTTGCACGTGATGCCATACCCGATAAAGACGCGCGGGGCGGCGAGGTGTATCTCCTTTCCG
GCCCGACAATGATGATGCCGGGCCACGGTCACGGGCATATGGAACACAGCAACTGGCGGATGATTAACTTGCCGGTTGGC
CCGTTGGTGGACGGCAAACCGATTTATACGCTCTACATCGCGCTTTCGATCGATTTTCATCTTCATTACATAAATGATTT
GATGAATAAACTTATTATGACCGCATCGATCATCAGCATCCTGATCGTCTTTATCGTGCTATTGGCGGTACATAAAGGTC
ACGCGCCGATCCGCAGCGTCAGCCGTCAAATCCAGAATATCACCTCGAAAGATCTCGACGTTCGCCTTGACCCGCAGACC
GTGCCGATTGAGCTGGAACAGCTGGTACTGTCGTTCAACCATATGATCGAGCGTATTGAGGATGTCTTTACTCGCCAGTC
CAATTTCTCAGCGGATATCGCTCACGAAATTCGTACACCAATTACGAATCTCATAACGCAAACCGAAATCGCCCTCAGCC
AGTCACGCAGCCAGAAGGAGCTGGAAGATGTGCTCTACTCTAATCTCGAAGAGCTGACGCGAATGGCGAAAATGGTCAGC
GATATGTTGTTTCTCGCCCAGGCCGATAACAACCAGTTAATCCCCGAAAAGAAAATGCTCAACCTGGCGGATGAAGTCGG
CAAAGTGTTCGATTTTTTCGAGGCGTTAGCGGAAGATCGCGGCGTGGAGTTGCGGTTTGTTGGTGACGAGTGTCAGGTTG
CGGGCGATCCGCTGATGCTGCGTCGGGCGTTAAACAACCTGCTTTCTAATGCCCTGCGTTATACGCCACCCGGAGAGGCT
ATTGTAGTACGCTGCCAGGCGGTCGATCATCAGGTGCAAGTTACCGTCGAAAACCCCGGTACGCCCATTGCGCCCGAACA
CTTACCGCGATTGTTTGACCGTTTCTACCGCGTTGATCCTTCCCGCCAGCGAAAAGGCGAAGGTAGCGGCATTGGGCTGG
CGATAGTGAAATCGATTGTTGTCGCGCATAAAGGCACAGTTGCGGTAACGTCAGATGCGCGAGGAACAAGGTTTGTGATT
GTGCTGCCGGAGAGGGAGTGA

Upstream 100 bases:

>100_bases
ACTAACGCCATTGATGTGGCGGTGAAGCGGCTGCGTGGCAAAATCGACAACGACTTTGAGCCGAAGCTGATTCAAACCGT
GCGCGGCGTGGGTTACATGC

Downstream 100 bases:

>100_bases
TAGGTCATATAACGCAATAGATATTATTTGAATTGAGAATTTGATATTTCGCCGGAAGAAAAAGCGTAAAATCCGTTTCG
TGCAGATGGTGAGTTTTATC

Product: sensor kinase CusS

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 486; Mature: 486

Protein sequence:

>486_residues
MRCRMVSKPFQRPFSLATRLTFFISLATIAAFFAFAWIMIHSVKVHFAEQDINDLKEISATLERVLNHPDETQARRLMTL
EDIVSGYSNVLISLADSHGKTVYHSPGAPDIREFARDAIPDKDARGGEVYLLSGPTMMMPGHGHGHMEHSNWRMINLPVG
PLVDGKPIYTLYIALSIDFHLHYINDLMNKLIMTASIISILIVFIVLLAVHKGHAPIRSVSRQIQNITSKDLDVRLDPQT
VPIELEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTPITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVS
DMLFLAQADNNQLIPEKKMLNLADEVGKVFDFFEALAEDRGVELRFVGDECQVAGDPLMLRRALNNLLSNALRYTPPGEA
IVVRCQAVDHQVQVTVENPGTPIAPEHLPRLFDRFYRVDPSRQRKGEGSGIGLAIVKSIVVAHKGTVAVTSDARGTRFVI
VLPERE

Sequences:

>Translated_486_residues
MRCRMVSKPFQRPFSLATRLTFFISLATIAAFFAFAWIMIHSVKVHFAEQDINDLKEISATLERVLNHPDETQARRLMTL
EDIVSGYSNVLISLADSHGKTVYHSPGAPDIREFARDAIPDKDARGGEVYLLSGPTMMMPGHGHGHMEHSNWRMINLPVG
PLVDGKPIYTLYIALSIDFHLHYINDLMNKLIMTASIISILIVFIVLLAVHKGHAPIRSVSRQIQNITSKDLDVRLDPQT
VPIELEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTPITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVS
DMLFLAQADNNQLIPEKKMLNLADEVGKVFDFFEALAEDRGVELRFVGDECQVAGDPLMLRRALNNLLSNALRYTPPGEA
IVVRCQAVDHQVQVTVENPGTPIAPEHLPRLFDRFYRVDPSRQRKGEGSGIGLAIVKSIVVAHKGTVAVTSDARGTRFVI
VLPERE
>Mature_486_residues
MRCRMVSKPFQRPFSLATRLTFFISLATIAAFFAFAWIMIHSVKVHFAEQDINDLKEISATLERVLNHPDETQARRLMTL
EDIVSGYSNVLISLADSHGKTVYHSPGAPDIREFARDAIPDKDARGGEVYLLSGPTMMMPGHGHGHMEHSNWRMINLPVG
PLVDGKPIYTLYIALSIDFHLHYINDLMNKLIMTASIISILIVFIVLLAVHKGHAPIRSVSRQIQNITSKDLDVRLDPQT
VPIELEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTPITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVS
DMLFLAQADNNQLIPEKKMLNLADEVGKVFDFFEALAEDRGVELRFVGDECQVAGDPLMLRRALNNLLSNALRYTPPGEA
IVVRCQAVDHQVQVTVENPGTPIAPEHLPRLFDRFYRVDPSRQRKGEGSGIGLAIVKSIVVAHKGTVAVTSDARGTRFVI
VLPERE

Specific function: Member of the two-component regulatory system CusS/CusR. Copper ion sensor. Could also be a silver ion sensor. Probably activates CusR by phosphorylation [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1786783, Length=479, Percent_Identity=97.4947807933194, Blast_Score=944, Evalue=0.0,
Organism=Escherichia coli, GI1788279, Length=298, Percent_Identity=33.2214765100671, Blast_Score=151, Evalue=1e-37,
Organism=Escherichia coli, GI1786600, Length=265, Percent_Identity=33.9622641509434, Blast_Score=137, Evalue=2e-33,
Organism=Escherichia coli, GI1788393, Length=271, Percent_Identity=30.9963099630996, Blast_Score=122, Evalue=7e-29,
Organism=Escherichia coli, GI1790346, Length=332, Percent_Identity=29.5180722891566, Blast_Score=116, Evalue=3e-27,
Organism=Escherichia coli, GI1787894, Length=311, Percent_Identity=29.2604501607717, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1789403, Length=282, Percent_Identity=31.2056737588652, Blast_Score=96, Evalue=6e-21,
Organism=Escherichia coli, GI145693157, Length=237, Percent_Identity=27.0042194092827, Blast_Score=92, Evalue=6e-20,
Organism=Escherichia coli, GI1790551, Length=296, Percent_Identity=28.0405405405405, Blast_Score=85, Evalue=1e-17,
Organism=Escherichia coli, GI1789149, Length=236, Percent_Identity=28.3898305084746, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1789808, Length=283, Percent_Identity=27.208480565371, Blast_Score=80, Evalue=4e-16,
Organism=Escherichia coli, GI87081816, Length=253, Percent_Identity=27.2727272727273, Blast_Score=79, Evalue=7e-16,
Organism=Escherichia coli, GI1790436, Length=220, Percent_Identity=25.9090909090909, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI1788713, Length=230, Percent_Identity=28.2608695652174, Blast_Score=75, Evalue=7e-15,
Organism=Escherichia coli, GI1787374, Length=348, Percent_Identity=22.7011494252874, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI1790861, Length=298, Percent_Identity=25.503355704698, Blast_Score=72, Evalue=1e-13,
Organism=Escherichia coli, GI87082128, Length=295, Percent_Identity=24.0677966101695, Blast_Score=69, Evalue=6e-13,
Organism=Escherichia coli, GI48994928, Length=238, Percent_Identity=25.6302521008403, Blast_Score=68, Evalue=2e-12,
Organism=Escherichia coli, GI1786912, Length=244, Percent_Identity=25.4098360655738, Blast_Score=67, Evalue=3e-12,
Organism=Escherichia coli, GI1788549, Length=223, Percent_Identity=26.0089686098655, Blast_Score=63, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR006290
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 54615; Mature: 54615

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRCRMVSKPFQRPFSLATRLTFFISLATIAAFFAFAWIMIHSVKVHFAEQDINDLKEISA
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECHHHHHHHHHHHH
TLERVLNHPDETQARRLMTLEDIVSGYSNVLISLADSHGKTVYHSPGAPDIREFARDAIP
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHEECCCCCEEEECCCCCHHHHHHHHCCC
DKDARGGEVYLLSGPTMMMPGHGHGHMEHSNWRMINLPVGPLVDGKPIYTLYIALSIDFH
CCCCCCCEEEEEECCEEECCCCCCCCCCCCCEEEEEECCCCCCCCCCEEEEEEEEEEEHH
LHYINDLMNKLIMTASIISILIVFIVLLAVHKGHAPIRSVSRQIQNITSKDLDVRLDPQT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCEEEECCCC
VPIELEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTPITNLITQTEIALSQSRSQKE
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHH
LEDVLYSNLEELTRMAKMVSDMLFLAQADNNQLIPEKKMLNLADEVGKVFDFFEALAEDR
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCC
GVELRFVGDECQVAGDPLMLRRALNNLLSNALRYTPPGEAIVVRCQAVDHQVQVTVENPG
CCEEEEECCHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCEEEEEEECCC
TPIAPEHLPRLFDRFYRVDPSRQRKGEGSGIGLAIVKSIVVAHKGTVAVTSDARGTRFVI
CCCCHHHHHHHHHHHHCCCCCHHCCCCCCCHHHHHHHHHHHHCCCCEEEECCCCCCEEEE
VLPERE
EECCCC
>Mature Secondary Structure
MRCRMVSKPFQRPFSLATRLTFFISLATIAAFFAFAWIMIHSVKVHFAEQDINDLKEISA
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECHHHHHHHHHHHH
TLERVLNHPDETQARRLMTLEDIVSGYSNVLISLADSHGKTVYHSPGAPDIREFARDAIP
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHEECCCCCEEEECCCCCHHHHHHHHCCC
DKDARGGEVYLLSGPTMMMPGHGHGHMEHSNWRMINLPVGPLVDGKPIYTLYIALSIDFH
CCCCCCCEEEEEECCEEECCCCCCCCCCCCCEEEEEECCCCCCCCCCEEEEEEEEEEEHH
LHYINDLMNKLIMTASIISILIVFIVLLAVHKGHAPIRSVSRQIQNITSKDLDVRLDPQT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCEEEECCCC
VPIELEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTPITNLITQTEIALSQSRSQKE
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHH
LEDVLYSNLEELTRMAKMVSDMLFLAQADNNQLIPEKKMLNLADEVGKVFDFFEALAEDR
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCC
GVELRFVGDECQVAGDPLMLRRALNNLLSNALRYTPPGEAIVVRCQAVDHQVQVTVENPG
CCEEEEECCHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCEEEEEEECCC
TPIAPEHLPRLFDRFYRVDPSRQRKGEGSGIGLAIVKSIVVAHKGTVAVTSDARGTRFVI
CCCCHHHHHHHHHHHHCCCCCHHCCCCCCCHHHHHHHHHHHHCCCCEEEECCCCCCEEEE
VLPERE
EECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]