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The map label for this gene is priC [H]

Identifier: 218703750

GI number: 218703750

Start: 548831

End: 549358

Strand: Reverse

Name: priC [H]

Synonym: ECUMN_0506

Alternate gene names: 218703750

Gene position: 549358-548831 (Counterclockwise)

Preceding gene: 218703759

Following gene: 218703749

Centisome position: 10.56

GC content: 59.66

Gene sequence:

>528_bases
GTGAAAACCGCCCTGCTGCTGGAAAAACTGGAAGGACAGCTCGCTACGCTGCGCCAGCGTTGTGCCCCGGTAGCGCAATT
TGCCACGCTAAGCGCCCGTTTTGACAGGCACCTTTTTCAGACTCGTGCGACAACACTGCAGGCTTGTCTCGATGAGGCAG
GCGATAACCTGGCTGCGCTTCGTCATGCCGTTGAGCAACAGCAACTGCCGCAAGTCGCCTGGCTGGCGGAACATCTGGCG
GCGCAACTGGAGGCTATAGCGCGTGAAGCCACAGCCTGGTCGCTACGCGAGTGGGACAGTGCGCCACCGAAAATTGCCCG
CTGGCAGCGTAAACGTATTCAGCATCAGGATTTTGAGCGGCGGCTAAGTGAGATGGTTGCTGAACGTCGAGCCCGCCTGG
CGCGGGTGACCGATCTCGTGGAACAGCAAACGCTGCATCGTGAAGTGGAAGCCTATGAAGCGCGCCTGGCACGCTGCCGC
CATGCGCTGGAAAAAATCGAAAACAGGCTAGCGCGCTTAACCCGCTAG

Upstream 100 bases:

>100_bases
AGCCAATGATGATTAAAATGATTCGTTGCATATTGAGTGTTGTCAGCTTACAGAGTGGCTACTTTAGCATAACAATTATC
ATTTTCATTGAGGTCTTATC

Downstream 100 bases:

>100_bases
CAATGGAGAGAGTATGTCACTGGAAAATGCCCCTGACGATGTCAAACTGGCTGTCGATTTGATTGTGCTGCTGGAAGAAA
ATCAGATCCCAGCCCGCACC

Product: primosomal replication protein N''

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 175; Mature: 175

Protein sequence:

>175_residues
MKTALLLEKLEGQLATLRQRCAPVAQFATLSARFDRHLFQTRATTLQACLDEAGDNLAALRHAVEQQQLPQVAWLAEHLA
AQLEAIAREATAWSLREWDSAPPKIARWQRKRIQHQDFERRLSEMVAERRARLARVTDLVEQQTLHREVEAYEARLARCR
HALEKIENRLARLTR

Sequences:

>Translated_175_residues
MKTALLLEKLEGQLATLRQRCAPVAQFATLSARFDRHLFQTRATTLQACLDEAGDNLAALRHAVEQQQLPQVAWLAEHLA
AQLEAIAREATAWSLREWDSAPPKIARWQRKRIQHQDFERRLSEMVAERRARLARVTDLVEQQTLHREVEAYEARLARCR
HALEKIENRLARLTR
>Mature_175_residues
MKTALLLEKLEGQLATLRQRCAPVAQFATLSARFDRHLFQTRATTLQACLDEAGDNLAALRHAVEQQQLPQVAWLAEHLA
AQLEAIAREATAWSLREWDSAPPKIARWQRKRIQHQDFERRLSEMVAERRARLARVTDLVEQQTLHREVEAYEARLARCR
HALEKIENRLARLTR

Specific function: PriA recognizes a specific hairpin sequence on bacteriophage phi X174 ssDNA. This structure is then recognized and bound by proteins priB and priC. Formation of the primosome proceeds with the subsequent actions of dnaB, dnaC, dnaT and primase [H]

COG id: COG3923

COG function: function code L; Primosomal replication protein N''

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786673, Length=175, Percent_Identity=98.2857142857143, Blast_Score=337, Evalue=4e-94,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010890 [H]

Pfam domain/function: PF07445 priB_priC [H]

EC number: NA

Molecular weight: Translated: 20305; Mature: 20305

Theoretical pI: Translated: 10.33; Mature: 10.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTALLLEKLEGQLATLRQRCAPVAQFATLSARFDRHLFQTRATTLQACLDEAGDNLAAL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
RHAVEQQQLPQVAWLAEHLAAQLEAIAREATAWSLREWDSAPPKIARWQRKRIQHQDFER
HHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
RLSEMVAERRARLARVTDLVEQQTLHREVEAYEARLARCRHALEKIENRLARLTR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKTALLLEKLEGQLATLRQRCAPVAQFATLSARFDRHLFQTRATTLQACLDEAGDNLAAL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
RHAVEQQQLPQVAWLAEHLAAQLEAIAREATAWSLREWDSAPPKIARWQRKRIQHQDFER
HHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
RLSEMVAERRARLARVTDLVEQQTLHREVEAYEARLARCRHALEKIENRLARLTR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1856227; 9278503 [H]