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The map label for this gene is proS [H]

Identifier: 218703449

GI number: 218703449

Start: 219908

End: 221626

Strand: Reverse

Name: proS [H]

Synonym: ECUMN_0192

Alternate gene names: 218703449

Gene position: 221626-219908 (Counterclockwise)

Preceding gene: 218703450

Following gene: 218703448

Centisome position: 4.26

GC content: 54.33

Gene sequence:

>1719_bases
ATGCGTACTAGCCAATACCTGCTCTCCACTCTCAAGGAGACACCTGCCGACGCCGAGGTGATCAGCCATCAGCTGATGCT
GCGCGCCGGGATGATCCGCAAGCTGGCCTCCGGGTTATATACCTGGCTGCCGACCGGCGTGCGCGTTCTGAAAAAAGTCG
AAAACATCGTGCGTGAAGAGATGAACAACGCCGGTGCGATCGAGGTGTCGATGCCGGTGGTTCAGCCAGCCGATTTGTGG
CAAGAGAGTGGTCGTTGGGAACAGTACGGTCCGGAACTGCTGCGTTTTGTTGACCGTGGCGAGCGTCCGTTCGTACTCGG
CCCAACTCATGAAGAAGTTATTACAGACCTGATTCGTAACGAGCTTAGCTCTTACAAACAGCTGCCGCTGAACTTCTATC
AGATCCAGACCAAGTTCCGCGACGAAGTGCGTCCGCGTTTCGGCGTCATGCGTTCCCGCGAATTCCTGATGAAAGATGCT
TACTCTTTCCATACTTCTCAGGAATCCCTGCAGGAAACCTACGATGCAATGTATGCGGCCTACAGCAAAATCTTCAGCCG
CATGGGGCTGGATTTCCGCGCCGTACAGGCCGACACCGGTTCTATCGGCGGTAGCGCCTCTCACGAATTCCAGGTGCTGG
CGCAGAGCGGTGAAGACGATGTGGTCTTCTCCGACACCTCTGACTATGCAGCGAACATTGAGCTGGCAGAAGCTATCGCG
CCGAAAGAACCGCGCGCTGCTGCTACCCAGGAAATGACGCTGGTTGATACGCCGAACGCGAAAACCATCGCGGAACTGGT
TGAACAGTTCAATCTGCCGATTGAGAAAACGGTGAAGACTCTGCTGGTTAAAGCGGTTGAAGGCAGTAGCTTCCCGCTGG
TTGCGCTGCTGGTGCGTGGTGACCACGAGCTGAACGAAGTTAAAGCAGAAAAACTGCCGCAGGTTGCAAGCCCGCTGACT
TTTGCGACCGAAGAAGAAATTCGTGCCGTGGTTAAAGCCGGTCCGGGTTCACTGGGTCCGGTAAACATGCCGATTCCGGT
GGTGATTGACCGTACCGTTGCAGCGATGAGTGATTTCGCTGCTGGTGCTAACATCGATGGTAAACACTACTTCGGCATCA
ACTGGGATCGCGATGTCGCTACCCCAGAAATTGCTGATATCCGTAACGTGGTGGCGGGCGATCCAAGCCCGGATGGCCAG
GGTACACTGCTGATCAAACGTGGTATCGAAGTTGGTCACATCTTCCAGCTGGGTACCAAGTACTCCGAAGCACTGAAAGC
CTCCGTACAGGGTGAAGATGGCCGTAATCAAATCCTGACGATGGGTTGCTACGGTATCGGAGTAACGCGTGTAGTAGCTG
CGGCGATTGAGCAGAACTACGACGAACGCGGCATCGTATGGCCGGACGCTATCGCACCGTTCCAGGTGGCGATTCTGCCG
ATGAACATGCACAAATCCTTCCGCGTACAGGAGCTTGCTGAGAAACTGTACAGCGAACTGCGCGCGCAGGGTATCGAAGT
GCTGCTGGATGACCGCAAAGAGCGTCCGGGCGTGATGTTTGCTGATATGGAACTGATCGGTATTCCGCACACTATTGTGC
TGGGCGACCGTAACCTCGACAACGACGATATCGAATATAAATATCGTCGTAACGGCGAGAAACAGTTAATTAAGACTGGT
GACATCGTCGATTATCTGGTGAAACAGATTAAAGGCTGA

Upstream 100 bases:

>100_bases
TTCTCTTTTGACATTTCTTTTGCACTGGTAAACTAAATCACTTTTTTTGTCCCAGGCTCACCTTGAGCCTGTTCTACCTT
CCAACTGGAACCGTAACAAC

Downstream 100 bases:

>100_bases
TGCCAGAAAGGGTCCTGAATTTCAGGGCCCTTTTTTACATGGCTTGTATAAAATGGATAAACCAAAGGCGCATTGCTGCC
TGCTTCTCCCCTGCTTTCTT

Product: prolyl-tRNA synthetase

Products: NA

Alternate protein names: Proline--tRNA ligase; ProRS [H]

Number of amino acids: Translated: 572; Mature: 572

Protein sequence:

>572_residues
MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLPTGVRVLKKVENIVREEMNNAGAIEVSMPVVQPADLW
QESGRWEQYGPELLRFVDRGERPFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
YSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGEDDVVFSDTSDYAANIELAEAIA
PKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEKTVKTLLVKAVEGSSFPLVALLVRGDHELNEVKAEKLPQVASPLT
FATEEEIRAVVKAGPGSLGPVNMPIPVVIDRTVAAMSDFAAGANIDGKHYFGINWDRDVATPEIADIRNVVAGDPSPDGQ
GTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTRVVAAAIEQNYDERGIVWPDAIAPFQVAILP
MNMHKSFRVQELAEKLYSELRAQGIEVLLDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTG
DIVDYLVKQIKG

Sequences:

>Translated_572_residues
MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLPTGVRVLKKVENIVREEMNNAGAIEVSMPVVQPADLW
QESGRWEQYGPELLRFVDRGERPFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
YSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGEDDVVFSDTSDYAANIELAEAIA
PKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEKTVKTLLVKAVEGSSFPLVALLVRGDHELNEVKAEKLPQVASPLT
FATEEEIRAVVKAGPGSLGPVNMPIPVVIDRTVAAMSDFAAGANIDGKHYFGINWDRDVATPEIADIRNVVAGDPSPDGQ
GTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTRVVAAAIEQNYDERGIVWPDAIAPFQVAILP
MNMHKSFRVQELAEKLYSELRAQGIEVLLDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTG
DIVDYLVKQIKG
>Mature_572_residues
MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLPTGVRVLKKVENIVREEMNNAGAIEVSMPVVQPADLW
QESGRWEQYGPELLRFVDRGERPFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
YSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGEDDVVFSDTSDYAANIELAEAIA
PKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEKTVKTLLVKAVEGSSFPLVALLVRGDHELNEVKAEKLPQVASPLT
FATEEEIRAVVKAGPGSLGPVNMPIPVVIDRTVAAMSDFAAGANIDGKHYFGINWDRDVATPEIADIRNVVAGDPSPDGQ
GTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTRVVAAAIEQNYDERGIVWPDAIAPFQVAILP
MNMHKSFRVQELAEKLYSELRAQGIEVLLDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTG
DIVDYLVKQIKG

Specific function: Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction:proline is first activated by ATP to form Pro- AMP and then transferred to the acceptor end of tRNA(Pro). As ProRS can inadvertently accommodate and process non-cognate amino acids su

COG id: COG0442

COG function: function code J; Prolyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI34303926, Length=226, Percent_Identity=45.575221238938, Blast_Score=218, Evalue=2e-56,
Organism=Escherichia coli, GI1786392, Length=572, Percent_Identity=99.3006993006993, Blast_Score=1168, Evalue=0.0,
Organism=Caenorhabditis elegans, GI115532348, Length=261, Percent_Identity=39.0804597701149, Blast_Score=180, Evalue=2e-45,
Organism=Caenorhabditis elegans, GI193203271, Length=89, Percent_Identity=38.2022471910112, Blast_Score=82, Evalue=5e-16,
Organism=Saccharomyces cerevisiae, GI6320931, Length=546, Percent_Identity=30.7692307692308, Blast_Score=253, Evalue=5e-68,
Organism=Drosophila melanogaster, GI24656200, Length=220, Percent_Identity=37.7272727272727, Blast_Score=178, Evalue=8e-45,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR002316
- InterPro:   IPR004500
- InterPro:   IPR007214 [H]

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; PF04073 YbaK [H]

EC number: =6.1.1.15 [H]

Molecular weight: Translated: 63623; Mature: 63623

Theoretical pI: Translated: 4.83; Mature: 4.83

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLPTGVRVLKKVENIVREE
CCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MNNAGAIEVSMPVVQPADLWQESGRWEQYGPELLRFVDRGERPFVLGPTHEEVITDLIRN
HCCCCEEEEECCCCCCHHHHHHCCCCHHHCHHHHHHHHCCCCCEEECCCHHHHHHHHHHH
ELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDAYSFHTSQESLQETYDAMYAA
HHHHHHHCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
YSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGEDDVVFSDTSDYAANIELAEAIA
HHHHHHHHCCCEEEEECCCCCCCCCCCCCHHEEECCCCCCEEEECCCCCCCCCCHHHHCC
PKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEKTVKTLLVKAVEGSSFPLVALLVRG
CCCCCCCCCCCEEEEECCCHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCEEEEEECC
DHELNEVKAEKLPQVASPLTFATEEEIRAVVKAGPGSLGPVNMPIPVVIDRTVAAMSDFA
CCHHHHHHHHHCHHHHCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCEEHHHHHHHHHHH
AGANIDGKHYFGINWDRDVATPEIADIRNVVAGDPSPDGQGTLLIKRGIEVGHIFQLGTK
CCCCCCCCEEECCCCCCCCCCCCHHHHHHHHCCCCCCCCCEEEEEECCCCCHHHHHHHHH
YSEALKASVQGEDGRNQILTMGCYGIGVTRVVAAAIEQNYDERGIVWPDAIAPFQVAILP
HHHHHHHHCCCCCCCCCEEEEEEHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEE
MNMHKSFRVQELAEKLYSELRAQGIEVLLDDRKERPGVMFADMELIGIPHTIVLGDRNLD
ECCCCCCCHHHHHHHHHHHHHHCCCCEEECCCCCCCCEEEECEEEECCCEEEEECCCCCC
NDDIEYKYRRNGEKQLIKTGDIVDYLVKQIKG
CCCCCCHHHCCCCHHHHHCCCHHHHHHHHHCC
>Mature Secondary Structure
MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLPTGVRVLKKVENIVREE
CCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MNNAGAIEVSMPVVQPADLWQESGRWEQYGPELLRFVDRGERPFVLGPTHEEVITDLIRN
HCCCCEEEEECCCCCCHHHHHHCCCCHHHCHHHHHHHHCCCCCEEECCCHHHHHHHHHHH
ELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDAYSFHTSQESLQETYDAMYAA
HHHHHHHCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
YSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGEDDVVFSDTSDYAANIELAEAIA
HHHHHHHHCCCEEEEECCCCCCCCCCCCCHHEEECCCCCCEEEECCCCCCCCCCHHHHCC
PKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEKTVKTLLVKAVEGSSFPLVALLVRG
CCCCCCCCCCCEEEEECCCHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCEEEEEECC
DHELNEVKAEKLPQVASPLTFATEEEIRAVVKAGPGSLGPVNMPIPVVIDRTVAAMSDFA
CCHHHHHHHHHCHHHHCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCEEHHHHHHHHHHH
AGANIDGKHYFGINWDRDVATPEIADIRNVVAGDPSPDGQGTLLIKRGIEVGHIFQLGTK
CCCCCCCCEEECCCCCCCCCCCCHHHHHHHHCCCCCCCCCEEEEEECCCCCHHHHHHHHH
YSEALKASVQGEDGRNQILTMGCYGIGVTRVVAAAIEQNYDERGIVWPDAIAPFQVAILP
HHHHHHHHCCCCCCCCCEEEEEEHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEE
MNMHKSFRVQELAEKLYSELRAQGIEVLLDDRKERPGVMFADMELIGIPHTIVLGDRNLD
ECCCCCCCHHHHHHHHHHHHHHCCCCEEECCCCCCCCEEEECEEEECCCEEEEECCCCCC
NDDIEYKYRRNGEKQLIKTGDIVDYLVKQIKG
CCCCCCHHHCCCCHHHHHCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA