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The map label for this gene is ecpD [H]

Identifier: 218703396

GI number: 218703396

Start: 159493

End: 160224

Strand: Reverse

Name: ecpD [H]

Synonym: ECUMN_0137

Alternate gene names: 218703396

Gene position: 160224-159493 (Counterclockwise)

Preceding gene: 218703397

Following gene: 218703395

Centisome position: 3.08

GC content: 44.95

Gene sequence:

>732_bases
TTGTTAAATAAAAAGAATATTGTCGCATTATGTGTTGCCAGTAGTATGGCATTTAGTATGCCTGTTATGGCTGATATTGT
TATTTCCGGTACACGCGTCATTTATAAAAGTGATCAAAAAAGCGTTAGCGTACGCCTGGAGAATAAAGGCAATAGCCATC
TGTTAGTACAAAGCTGGCTGGATACCGGTGACGATAACGCTGAGCCGGGAAGCATTAATGTACCATTTACGGCAACACCT
CCCGTTTCACGTATTGATGCTAAACGCGGTCAAACCATCAAATTGATGTATACCGGCAGCACCGTACTCCCCAGCGACAA
AGAAAGCGTATTCTGGTTTAACGTGCTGGAAGTGCCCCCAAAACCAGATGCTTCAAAAGTAGAGAATCAGAGCCTGTTAC
AACTGGCTTTCCGTACCCGTATCAAGCTGTTCTATCGTCCGGCAGGATTAAACGGTAACCCGTCTGAAGCACCATTAGCC
CTGAAATGGAAATGGGCTGAAGGGAAGTCTGGCCTGAGTGTATATAACCCAACATCTTATTACGTCTCTTTCAACAGTGC
AGAGTTAATCGCTGGCGGTAAGTCGAACCCGCTCAATGTAAAAATGGTTGCACCGAAAAGCGAAGAGATTTTCACCATAA
GCGGTTCAGGCGGGAAAGCAACGTCAGGAAAGGTACATTTCTCCGCGATTAATGATTTTGGCGGCGCAATTGAAGGCGAT
GTCAATTTGTAA

Upstream 100 bases:

>100_bases
ACAATTAAGTTATTCGTTGGTGATTTAAATTACTACAAGGGTGCGACACCTATTGTCGCCCCCTTGTCTTGAAAACAATT
CATGTCATGGATGGTATATT

Downstream 100 bases:

>100_bases
GACCAGGGACCTTATGCAGCAGGAAGCCAATGTAATGAAAACAAAGACATCGACTAAACGCCATAATCTTACGCGTATCG
CAATGTATTGCTCAATCATG

Product: putative chaperone protein EcpD

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 243; Mature: 243

Protein sequence:

>243_residues
MLNKKNIVALCVASSMAFSMPVMADIVISGTRVIYKSDQKSVSVRLENKGNSHLLVQSWLDTGDDNAEPGSINVPFTATP
PVSRIDAKRGQTIKLMYTGSTVLPSDKESVFWFNVLEVPPKPDASKVENQSLLQLAFRTRIKLFYRPAGLNGNPSEAPLA
LKWKWAEGKSGLSVYNPTSYYVSFNSAELIAGGKSNPLNVKMVAPKSEEIFTISGSGGKATSGKVHFSAINDFGGAIEGD
VNL

Sequences:

>Translated_243_residues
MLNKKNIVALCVASSMAFSMPVMADIVISGTRVIYKSDQKSVSVRLENKGNSHLLVQSWLDTGDDNAEPGSINVPFTATP
PVSRIDAKRGQTIKLMYTGSTVLPSDKESVFWFNVLEVPPKPDASKVENQSLLQLAFRTRIKLFYRPAGLNGNPSEAPLA
LKWKWAEGKSGLSVYNPTSYYVSFNSAELIAGGKSNPLNVKMVAPKSEEIFTISGSGGKATSGKVHFSAINDFGGAIEGD
VNL
>Mature_243_residues
MLNKKNIVALCVASSMAFSMPVMADIVISGTRVIYKSDQKSVSVRLENKGNSHLLVQSWLDTGDDNAEPGSINVPFTATP
PVSRIDAKRGQTIKLMYTGSTVLPSDKESVFWFNVLEVPPKPDASKVENQSLLQLAFRTRIKLFYRPAGLNGNPSEAPLA
LKWKWAEGKSGLSVYNPTSYYVSFNSAELIAGGKSNPLNVKMVAPKSEEIFTISGSGGKATSGKVHFSAINDFGGAIEGD
VNL

Specific function: Probably mediates assembly of a pilin [H]

COG id: COG3121

COG function: function code NU; P pilus assembly protein, chaperone PapD

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Ig-like (immunoglobulin-like) domain [H]

Homologues:

Organism=Escherichia coli, GI1786333, Length=245, Percent_Identity=75.5102040816327, Blast_Score=376, Evalue=1e-106,
Organism=Escherichia coli, GI1788428, Length=246, Percent_Identity=34.5528455284553, Blast_Score=130, Evalue=6e-32,
Organism=Escherichia coli, GI1789532, Length=230, Percent_Identity=35.6521739130435, Blast_Score=129, Evalue=1e-31,
Organism=Escherichia coli, GI1790771, Length=241, Percent_Identity=31.1203319502075, Blast_Score=112, Evalue=3e-26,
Organism=Escherichia coli, GI1786743, Length=213, Percent_Identity=33.8028169014084, Blast_Score=104, Evalue=6e-24,
Organism=Escherichia coli, GI1786936, Length=227, Percent_Identity=28.1938325991189, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI87081806, Length=240, Percent_Identity=33.75, Blast_Score=101, Evalue=4e-23,
Organism=Escherichia coli, GI1788677, Length=188, Percent_Identity=35.1063829787234, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI1787171, Length=211, Percent_Identity=28.9099526066351, Blast_Score=81, Evalue=8e-17,
Organism=Escherichia coli, GI87082203, Length=249, Percent_Identity=26.5060240963855, Blast_Score=71, Evalue=7e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008962
- InterPro:   IPR001829
- InterPro:   IPR016148
- InterPro:   IPR018046
- InterPro:   IPR016147 [H]

Pfam domain/function: PF02753 Pili_assembly_C; PF00345 Pili_assembly_N [H]

EC number: NA

Molecular weight: Translated: 26202; Mature: 26202

Theoretical pI: Translated: 9.70; Mature: 9.70

Prosite motif: PS00635 PILI_CHAPERONE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLNKKNIVALCVASSMAFSMPVMADIVISGTRVIYKSDQKSVSVRLENKGNSHLLVQSWL
CCCCCCEEEEEEECCHHHCCCEEEEEEEECEEEEEECCCCEEEEEEECCCCCEEEEEEEC
DTGDDNAEPGSINVPFTATPPVSRIDAKRGQTIKLMYTGSTVLPSDKESVFWFNVLEVPP
CCCCCCCCCCEEECCEECCCCHHHHHCCCCCEEEEEEECCEECCCCCCCEEEEEEEECCC
KPDASKVENQSLLQLAFRTRIKLFYRPAGLNGNPSEAPLALKWKWAEGKSGLSVYNPTSY
CCCHHHCCCHHHHHHHHHHEEEEEEEECCCCCCCCCCCEEEEEEECCCCCCCEEECCEEE
YVSFNSAELIAGGKSNPLNVKMVAPKSEEIFTISGSGGKATSGKVHFSAINDFGGAIEGD
EEEECCEEEEECCCCCCEEEEEECCCCCEEEEEECCCCCCCCCEEEEEEECCCCCEEECC
VNL
CCC
>Mature Secondary Structure
MLNKKNIVALCVASSMAFSMPVMADIVISGTRVIYKSDQKSVSVRLENKGNSHLLVQSWL
CCCCCCEEEEEEECCHHHCCCEEEEEEEECEEEEEECCCCEEEEEEECCCCCEEEEEEEC
DTGDDNAEPGSINVPFTATPPVSRIDAKRGQTIKLMYTGSTVLPSDKESVFWFNVLEVPP
CCCCCCCCCCEEECCEECCCCHHHHHCCCCCEEEEEEECCEECCCCCCCEEEEEEEECCC
KPDASKVENQSLLQLAFRTRIKLFYRPAGLNGNPSEAPLALKWKWAEGKSGLSVYNPTSY
CCCHHHCCCHHHHHHHHHHEEEEEEEECCCCCCCCCCCEEEEEEECCCCCCCEEECCEEE
YVSFNSAELIAGGKSNPLNVKMVAPKSEEIFTISGSGGKATSGKVHFSAINDFGGAIEGD
EEEECCEEEEECCCCCCEEEEEECCCCCEEEEEECCCCCCCCCEEEEEEECCCCCEEECC
VNL
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8102362; 8202364; 9278503 [H]