Click here to switch to the map view.

The map label for this gene is uxuB

Identifier: 218703022

GI number: 218703022

Start: 5000361

End: 5001821

Strand: Direct

Name: uxuB

Synonym: ECIAI39_4796

Alternate gene names: 218703022

Gene position: 5000361-5001821 (Clockwise)

Preceding gene: 218703021

Following gene: 218703023

Centisome position: 97.43

GC content: 57.49

Gene sequence:

>1461_bases
ATGACTACTATTGTTGACAGCAATCTGCCGGTTGCCCGCCCGTCATGGGATCATTCTCGTCTGGAATCACGCATTGTGCA
TCTCGGTTGCGGGGCGTTTCACCGCGCGCACCAGGCGCTGTATACCCATCATCTGCTGGAAAGCACCGACAGCGACTGGG
GTATCTGCGAAGTTAACCTGATGCCAGGTAACGACCGCGTGCTGATCGAAAACCTGAAAAAACAGCAACTGCTGTACACC
GTAGCGGAAAAAGGCGCAGAGAGCACCGAGCTGAAAATTATCGGTTCGATGAAAGAAGCGCTGCATCCGGAAATCGATGG
CTGCGAAGGTATTCTCAACGCGATGGCGCGTCCGCAAACGGCGATTGTCTCTCTAACGGTCACGGAAAAAGGCTACTGCG
CTGATGCGGCAAGCGGTCAACTGGATCTCAATAACCCGCTGATCAAGCACGATCTGGAAAACCCGACTGCGCCGAAGTCC
GCGATTGGTTACATCGTCGAAGCCTTGCGTCTGCGTCGTGAAAAAGGGTTGAAAGCGTTTACGGTGATGTCCTGCGATAA
CGTGCGTGAAAACGGTCATGTGGCGAAGGTCGCGGTACTGGGGCTGGCTCAGGCGCGTGACCCGCAGCTGGCGGCATGGA
TTGAAGAAAATGTCACCTTCCCGTGCACCATGGTTGACCGCATCGTTCCGGCGGCGACGCCAGAAACCTTACAGGAAATT
GCTGACCAGCTGGGTGTTTACGACCCGTGCGCCATTGCCTGCGAACCGTTCCGTCAGTGGGTGATTGAAGATAACTTCGT
TAATGGTCGCCCGGACTGGGATAAAGTGGGCGCACAGTTCGTTGCAGACGTTGTGCCGTTCGAAATGATGAAGCTGCGTA
TGCTGAACGGCAGCCACTCTTTTCTGGCGTACCTCGGTTACCTCGGCGGCTATGAAACCATTGCCGACACCATGACTAAC
CCGGCTTATCGCAAAGCGGCCTTCTCCCTGATGATGCAGGAACAAGCGCCAACGCTGTCGATGCCGGAAGGTACGGACCT
GAACGCCTATGCGACGTTGCTGATCGAGCGTTTCAGCAACCCGTCTCTGCGTCACCGTACCTGGCAGATTGCGATGGACG
GCAGCCAGAAGTTACCGCAGCGTCTGCTGGACCCGGTGCGTCTGCACCTGCAAAACGGCGGCAGCTGGCGTCACCTGGCG
CTGGGCGTGGCTGGCTGGATGCGTTACACCCAGGGCGTGGATGAGCAGGGTAATGCCATTGACGTGGTCGACCCGATGCT
GGCGGAGTTCCAGAAGATCAACGCGCAGTATCAGGGCGCAGACCGCGTGAAAGCGCTGCTGGGCCTGAGCGGTATTTTTG
CCGATGATCTGCCGCAGAATGCCGACTTTGTTGGCGCAGTGACGGCGGCATATCAGCAGCTGTGCGAACGCGGTGCGCGC
GAGTGTGTGGCTGCGCTGTAA

Upstream 100 bases:

>100_bases
GCGCTTTCTTTAGCCGTTAATATCCACCGGCATGGCTGCGCGCCGTGCCGGTTCCTTCTTCCTTGCCGTCACTCTCTGAA
GACGGATTCTGGAGTTTACG

Downstream 100 bases:

>100_bases
CTAACTGATTACCCTACAGACTTACTGGTCAATCAAACTGATATTTGGTTGACCAGTTTTCGTTTTTTTGCCCACCTGTA
CGTGCCAACTTCCAGTGCTA

Product: D-mannonate oxidoreductase, NAD-binding

Products: NA

Alternate protein names: Fructuronate reductase [H]

Number of amino acids: Translated: 486; Mature: 485

Protein sequence:

>486_residues
MTTIVDSNLPVARPSWDHSRLESRIVHLGCGAFHRAHQALYTHHLLESTDSDWGICEVNLMPGNDRVLIENLKKQQLLYT
VAEKGAESTELKIIGSMKEALHPEIDGCEGILNAMARPQTAIVSLTVTEKGYCADAASGQLDLNNPLIKHDLENPTAPKS
AIGYIVEALRLRREKGLKAFTVMSCDNVRENGHVAKVAVLGLAQARDPQLAAWIEENVTFPCTMVDRIVPAATPETLQEI
ADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDKVGAQFVADVVPFEMMKLRMLNGSHSFLAYLGYLGGYETIADTMTN
PAYRKAAFSLMMQEQAPTLSMPEGTDLNAYATLLIERFSNPSLRHRTWQIAMDGSQKLPQRLLDPVRLHLQNGGSWRHLA
LGVAGWMRYTQGVDEQGNAIDVVDPMLAEFQKINAQYQGADRVKALLGLSGIFADDLPQNADFVGAVTAAYQQLCERGAR
ECVAAL

Sequences:

>Translated_486_residues
MTTIVDSNLPVARPSWDHSRLESRIVHLGCGAFHRAHQALYTHHLLESTDSDWGICEVNLMPGNDRVLIENLKKQQLLYT
VAEKGAESTELKIIGSMKEALHPEIDGCEGILNAMARPQTAIVSLTVTEKGYCADAASGQLDLNNPLIKHDLENPTAPKS
AIGYIVEALRLRREKGLKAFTVMSCDNVRENGHVAKVAVLGLAQARDPQLAAWIEENVTFPCTMVDRIVPAATPETLQEI
ADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDKVGAQFVADVVPFEMMKLRMLNGSHSFLAYLGYLGGYETIADTMTN
PAYRKAAFSLMMQEQAPTLSMPEGTDLNAYATLLIERFSNPSLRHRTWQIAMDGSQKLPQRLLDPVRLHLQNGGSWRHLA
LGVAGWMRYTQGVDEQGNAIDVVDPMLAEFQKINAQYQGADRVKALLGLSGIFADDLPQNADFVGAVTAAYQQLCERGAR
ECVAAL
>Mature_485_residues
TTIVDSNLPVARPSWDHSRLESRIVHLGCGAFHRAHQALYTHHLLESTDSDWGICEVNLMPGNDRVLIENLKKQQLLYTV
AEKGAESTELKIIGSMKEALHPEIDGCEGILNAMARPQTAIVSLTVTEKGYCADAASGQLDLNNPLIKHDLENPTAPKSA
IGYIVEALRLRREKGLKAFTVMSCDNVRENGHVAKVAVLGLAQARDPQLAAWIEENVTFPCTMVDRIVPAATPETLQEIA
DQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDKVGAQFVADVVPFEMMKLRMLNGSHSFLAYLGYLGGYETIADTMTNP
AYRKAAFSLMMQEQAPTLSMPEGTDLNAYATLLIERFSNPSLRHRTWQIAMDGSQKLPQRLLDPVRLHLQNGGSWRHLAL
GVAGWMRYTQGVDEQGNAIDVVDPMLAEFQKINAQYQGADRVKALLGLSGIFADDLPQNADFVGAVTAAYQQLCERGARE
CVAAL

Specific function: Hexuronate conversion to KDG. [C]

COG id: COG0246

COG function: function code G; Mannitol-1-phosphate/altronate dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mannitol dehydrogenase family. UxuB subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790779, Length=486, Percent_Identity=99.5884773662551, Blast_Score=1010, Evalue=0.0,
Organism=Escherichia coli, GI1788497, Length=485, Percent_Identity=56.2886597938144, Blast_Score=558, Evalue=1e-160,
Organism=Escherichia coli, GI1787823, Length=472, Percent_Identity=58.2627118644068, Blast_Score=552, Evalue=1e-158,
Organism=Escherichia coli, GI48994885, Length=493, Percent_Identity=25.5578093306288, Blast_Score=105, Evalue=5e-24,
Organism=Escherichia coli, GI1790028, Length=252, Percent_Identity=28.1746031746032, Blast_Score=90, Evalue=3e-19,
Organism=Saccharomyces cerevisiae, GI6324401, Length=476, Percent_Identity=39.4957983193277, Blast_Score=359, Evalue=1e-100,
Organism=Saccharomyces cerevisiae, GI6320765, Length=476, Percent_Identity=39.4957983193277, Blast_Score=359, Evalue=1e-100,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008927
- InterPro:   IPR013328
- InterPro:   IPR000669
- InterPro:   IPR013118
- InterPro:   IPR023027
- InterPro:   IPR013131
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01232 Mannitol_dh; PF08125 Mannitol_dh_C [H]

EC number: =1.1.1.57 [H]

Molecular weight: Translated: 53629; Mature: 53498

Theoretical pI: Translated: 5.20; Mature: 5.20

Prosite motif: PS00974 MANNITOL_DHGENASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTIVDSNLPVARPSWDHSRLESRIVHLGCGAFHRAHQALYTHHLLESTDSDWGICEVNL
CCEEECCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEE
MPGNDRVLIENLKKQQLLYTVAEKGAESTELKIIGSMKEALHPEIDGCEGILNAMARPQT
ECCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEEHHHHHHCCCCCHHHHHHHHHCCCCE
AIVSLTVTEKGYCADAASGQLDLNNPLIKHDLENPTAPKSAIGYIVEALRLRREKGLKAF
EEEEEEEECCCCCCCCCCCCEECCCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCEE
TVMSCDNVRENGHVAKVAVLGLAQARDPQLAAWIEENVTFPCTMVDRIVPAATPETLQEI
EEEECCCCCCCCCEEEHHHHHHHHCCCCHHHHHHHCCCCCCHHHHHHHCCCCCHHHHHHH
ADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDKVGAQFVADVVPFEMMKLRMLNGSHS
HHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHH
FLAYLGYLGGYETIADTMTNPAYRKAAFSLMMQEQAPTLSMPEGTDLNAYATLLIERFSN
HHHHHHHHCCHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCC
PSLRHRTWQIAMDGSQKLPQRLLDPVRLHLQNGGSWRHLALGVAGWMRYTQGVDEQGNAI
CCCCCEEEEEEECCHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHCCCCCCCCEE
DVVDPMLAEFQKINAQYQGADRVKALLGLSGIFADDLPQNADFVGAVTAAYQQLCERGAR
CHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHH
ECVAAL
HHHHCC
>Mature Secondary Structure 
TTIVDSNLPVARPSWDHSRLESRIVHLGCGAFHRAHQALYTHHLLESTDSDWGICEVNL
CEEECCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEE
MPGNDRVLIENLKKQQLLYTVAEKGAESTELKIIGSMKEALHPEIDGCEGILNAMARPQT
ECCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEEHHHHHHCCCCCHHHHHHHHHCCCCE
AIVSLTVTEKGYCADAASGQLDLNNPLIKHDLENPTAPKSAIGYIVEALRLRREKGLKAF
EEEEEEEECCCCCCCCCCCCEECCCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCEE
TVMSCDNVRENGHVAKVAVLGLAQARDPQLAAWIEENVTFPCTMVDRIVPAATPETLQEI
EEEECCCCCCCCCEEEHHHHHHHHCCCCHHHHHHHCCCCCCHHHHHHHCCCCCHHHHHHH
ADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDKVGAQFVADVVPFEMMKLRMLNGSHS
HHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHH
FLAYLGYLGGYETIADTMTNPAYRKAAFSLMMQEQAPTLSMPEGTDLNAYATLLIERFSN
HHHHHHHHCCHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCC
PSLRHRTWQIAMDGSQKLPQRLLDPVRLHLQNGGSWRHLALGVAGWMRYTQGVDEQGNAI
CCCCCEEEEEEECCHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHCCCCCCCCEE
DVVDPMLAEFQKINAQYQGADRVKALLGLSGIFADDLPQNADFVGAVTAAYQQLCERGAR
CHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHH
ECVAAL
HHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7610040; 9278503 [H]