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The map label for this gene is insO

Identifier: 218702991

GI number: 218702991

Start: 4967709

End: 4968308

Strand: Direct

Name: insO

Synonym: ECIAI39_4763

Alternate gene names: 218702991

Gene position: 4967709-4968308 (Clockwise)

Preceding gene: 218702990

Following gene: 218702992

Centisome position: 96.8

GC content: 56.5

Gene sequence:

>600_bases
GTGGCCACTCTCTGCCATGTGTTCGGGGTCCATCGCAGCAGCTACAAATACTGGAAAAACCGTCCTGAAAAACCAGACGG
CAGACGGGCTGTATTACGTAGCCAGGTACTTGAACTGCATGGCATCAGCCACGGCTCTGCCGGAGCAAGAAGCATCGCCA
CAATGGCAACCCAGAGAGGCTACCAGATGGGGCACTGGCTTGCTGGCAGACTCATGAAAGAGCTGGGGCTGGTCAGTTGC
CAGCAGCCGACTCACCGGTATAAGCGTGGCGGTCATGAGCACGTTGCTATCCCGAATCATCTTGAGCGACAGTTCGCCGT
AACGGAACCAAATCAGGTGTGGTGCGGTGATGTGACCTATATCTGGACGGGTAAGCGCTGGGCGTACCTCGCCGTTGTTC
TCGACCTGTTCGCAAGAAAACCAGTGGGCTGGGCCATGTCGTTCTCGCCGGACAGCAGGCTTACCATGAAAGCACTGGAA
ATGGCATGGGAAACCCGTGGTAAGCCCGTCGGGGTGATGTTCCAGGCGATCAAGGCAGTCATTATACGAGCAGGCAGTTC
CGGCAGTTACTGTGGCGATACCGGATCAGGCAGAGTATGA

Upstream 100 bases:

>100_bases
ATTGAAATGGAAAATGAAATATTAAAAAGGCTACCGCGCTCTTGATGTCAGACTCCCTGAACAGTTTTCGATAATCGGGA
AACTCAGGGCGCGTTATCCT

Downstream 100 bases:

>100_bases
GTCGGCGTGGAAACTGCTGGGATAACAGCCCAATGGAGCGCTTCTTCAGGAGTCTGAAGAACGAATGGGTGCCAGCGACG
GGCTATGTAAGCTTCAGCGA

Product: transposase ORF B (fragment), IS911

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 199; Mature: 198

Protein sequence:

>199_residues
MATLCHVFGVHRSSYKYWKNRPEKPDGRRAVLRSQVLELHGISHGSAGARSIATMATQRGYQMGHWLAGRLMKELGLVSC
QQPTHRYKRGGHEHVAIPNHLERQFAVTEPNQVWCGDVTYIWTGKRWAYLAVVLDLFARKPVGWAMSFSPDSRLTMKALE
MAWETRGKPVGVMFQAIKAVIIRAGSSGSYCGDTGSGRV

Sequences:

>Translated_199_residues
MATLCHVFGVHRSSYKYWKNRPEKPDGRRAVLRSQVLELHGISHGSAGARSIATMATQRGYQMGHWLAGRLMKELGLVSC
QQPTHRYKRGGHEHVAIPNHLERQFAVTEPNQVWCGDVTYIWTGKRWAYLAVVLDLFARKPVGWAMSFSPDSRLTMKALE
MAWETRGKPVGVMFQAIKAVIIRAGSSGSYCGDTGSGRV
>Mature_198_residues
ATLCHVFGVHRSSYKYWKNRPEKPDGRRAVLRSQVLELHGISHGSAGARSIATMATQRGYQMGHWLAGRLMKELGLVSCQ
QPTHRYKRGGHEHVAIPNHLERQFAVTEPNQVWCGDVTYIWTGKRWAYLAVVLDLFARKPVGWAMSFSPDSRLTMKALEM
AWETRGKPVGVMFQAIKAVIIRAGSSGSYCGDTGSGRV

Specific function: Involved in the transposition of the insertion sequence IS911B [H]

COG id: COG2801

COG function: function code L; Transposase and inactivated derivatives

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 integrase catalytic domain [H]

Homologues:

Organism=Homo sapiens, GI310129419, Length=184, Percent_Identity=27.1739130434783, Blast_Score=82, Evalue=2e-16,
Organism=Escherichia coli, GI1788405, Length=198, Percent_Identity=29.2929292929293, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1787263, Length=198, Percent_Identity=29.2929292929293, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1786753, Length=198, Percent_Identity=29.2929292929293, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1786570, Length=198, Percent_Identity=29.2929292929293, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1786490, Length=198, Percent_Identity=29.2929292929293, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1789981, Length=165, Percent_Identity=28.4848484848485, Blast_Score=70, Evalue=8e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001584
- InterPro:   IPR012337 [H]

Pfam domain/function: PF00665 rve [H]

EC number: NA

Molecular weight: Translated: 22256; Mature: 22125

Theoretical pI: Translated: 10.56; Mature: 10.56

Prosite motif: PS50994 INTEGRASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATLCHVFGVHRSSYKYWKNRPEKPDGRRAVLRSQVLELHGISHGSAGARSIATMATQRG
CCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCC
YQMGHWLAGRLMKELGLVSCQQPTHRYKRGGHEHVAIPNHLERQFAVTEPNQVWCGDVTY
CHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCEEECCCHHHHCEECCCCCEEEECCEEE
IWTGKRWAYLAVVLDLFARKPVGWAMSFSPDSRLTMKALEMAWETRGKPVGVMFQAIKAV
EEECCHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHH
IIRAGSSGSYCGDTGSGRV
HHCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
ATLCHVFGVHRSSYKYWKNRPEKPDGRRAVLRSQVLELHGISHGSAGARSIATMATQRG
CHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCC
YQMGHWLAGRLMKELGLVSCQQPTHRYKRGGHEHVAIPNHLERQFAVTEPNQVWCGDVTY
CHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCEEECCCHHHHCEECCCCCEEEECCEEE
IWTGKRWAYLAVVLDLFARKPVGWAMSFSPDSRLTMKALEMAWETRGKPVGVMFQAIKAV
EEECCHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHH
IIRAGSSGSYCGDTGSGRV
HHCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7610040; 9278503 [H]