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The map label for this gene is dnaB

Identifier: 218702716

GI number: 218702716

Start: 4673306

End: 4674721

Strand: Direct

Name: dnaB

Synonym: ECIAI39_4472

Alternate gene names: 218702716

Gene position: 4673306-4674721 (Clockwise)

Preceding gene: 218702714

Following gene: 218702717

Centisome position: 91.06

GC content: 53.6

Gene sequence:

>1416_bases
ATGGCAGGAAATAAACCCTTCAACAAACAGCAGGCTGAACCCCGCGAACGCGATCCACAAGTTGCCGGGCTGAAAGTGCC
TCCGCACTCGATCGAAGCGGAGCAGTCGGTGTTGGGCGGTTTAATGCTGGATAACGAACGCTGGGACGATGTAGCCGAGC
GTGTGGTGGCAGACGATTTTTACACCCGCCCACACCGTCATATCTTTACTGAAATGGCGCGTTTACAGGAAAGCGGTAGT
CCTATCGATCTGATTACCCTTACGGAATCGCTGGAACGCCAGGGGCAACTTGATAGCGTCGGTGGTTTCGCTTATCTGGC
GGAGCTGTCAAAAAATACGCCAAGTGCGGCGAACATCAGTGCTTATGCTGACATCGTGCGTGAACGTGCCGTTGTTCGCG
AGATGATTTCGGTTGCGAATGAGATTGCCGAAGCCGGTTTTGATCCGCAGGGGCGTACCAGCGAAGATCTGCTGGACCTG
GCTGAATCCCGCGTCTTTAAAATTGCCGAAAGTCGTGCAAACAAAGACGAAGGGCCGAAGAACATCGCCGATGTGCTCGA
CGCCACGGTGGCGCGTATTGAGCAGTTGTTTCAGCAGCCACACGATGGCGTTACCGGGGTAAACACCGGTTATGACGATC
TCAACAAAAAAACCGCTGGCTTGCAGCCGTCGGATTTGATCATCGTCGCCGCGCGTCCGTCGATGGGTAAAACCACATTT
GCGATGAACCTCGTCGAAAACGCGGCGATGTTGCAGGATAAACCAGTACTTATCTTCTCGCTGGAGATGCCATCAGAACA
GATTATGATGCGTTCTTTGGCGTCGCTGTCGCGCGTTGACCAGACTAAAATCCGTACCGGGCAGCTCGATGATGAAGACT
GGGCACGTATTTCCGGCACCATGGGTATTTTGCTCGAAAAACGTAATATCTATATCGATGATTCCTCCGGCCTGACGCCA
ACGGAAGTGCGTTCCCGCGCGCGCCGTATTGCCCGTGAACACGGCGGCATCGGGCTTATCATGATCGACTACCTGCAACT
GATGCGCGTACCGGCGCTTTCCGATAACCGTACGCTGGAAATTGCAGAAATCTCCCGCTCGCTGAAAGCACTGGCGAAAG
AACTGAACGTGCCGGTGGTGGCGCTGTCCCAGTTGAACCGTTCTCTGGAACAACGTGCCGACAAACGCCCGGTCAACTCC
GACCTGCGTGAATCTGGCTCTATCGAGCAGGATGCGGACTTGATCATGTTTATCTATCGTGATGAGGTGTATCACGAAAA
CAGTGATTTAAAAGGCATCGCGGAAATTATTATCGGTAAACAACGTAACGGCCCAATCGGGACGGTACGCCTGACCTTTA
ACGGTCAATGGTCGCGCTTCGACAACTATGCGGGGCCGCTGTACGACGACGAATAA

Upstream 100 bases:

>100_bases
TTCAATTCGTGTTGCCATGTGTCCTCCAGAACGTATCGTCAGGGTCTGCTTCATATGGTAAAGTTTCGACCCATTCTTTA
TCTCGGTAACTCCATTCACT

Downstream 100 bases:

>100_bases
TAATTATTTTATGAATTAGGTAATTAAAGCAAACACTTATCAAGGAACACAAATGCAAGCGGCAACTGTTGTGATTAACC
GCCGCGCTCTGCGACACAAC

Product: replicative DNA helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 471; Mature: 470

Protein sequence:

>471_residues
MAGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLDNERWDDVAERVVADDFYTRPHRHIFTEMARLQESGS
PIDLITLTESLERQGQLDSVGGFAYLAELSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKTAGLQPSDLIIVAARPSMGKTTF
AMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASLSRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTP
TEVRSRARRIAREHGGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQRADKRPVNS
DLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVRLTFNGQWSRFDNYAGPLYDDE

Sequences:

>Translated_471_residues
MAGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLDNERWDDVAERVVADDFYTRPHRHIFTEMARLQESGS
PIDLITLTESLERQGQLDSVGGFAYLAELSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKTAGLQPSDLIIVAARPSMGKTTF
AMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASLSRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTP
TEVRSRARRIAREHGGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQRADKRPVNS
DLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVRLTFNGQWSRFDNYAGPLYDDE
>Mature_470_residues
AGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLDNERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSP
IDLITLTESLERQGQLDSVGGFAYLAELSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDLA
ESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKTAGLQPSDLIIVAARPSMGKTTFA
MNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASLSRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPT
EVRSRARRIAREHGGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQRADKRPVNSD
LRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVRLTFNGQWSRFDNYAGPLYDDE

Specific function: Participates in initiation and elongation during chromosome replication; it exhibits DNA-dependent ATPase activity and contains distinct active sites for ATP binding, DNA binding, and interaction with dnaC protein, primase, and other prepriming proteins [

COG id: COG0305

COG function: function code L; Replicative DNA helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 SF4 helicase domain [H]

Homologues:

Organism=Escherichia coli, GI1790486, Length=471, Percent_Identity=99.5753715498938, Blast_Score=954, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR007692
- InterPro:   IPR007694
- InterPro:   IPR007693
- InterPro:   IPR016136 [H]

Pfam domain/function: PF00772 DnaB; PF03796 DnaB_C [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 52406; Mature: 52275

Theoretical pI: Translated: 4.68; Mature: 4.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLDNERWDDVAERVVADDF
CCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCHHHHHHCCCEECCCHHHHHHHHHHHHHH
YTRPHRHIFTEMARLQESGSPIDLITLTESLERQGQLDSVGGFAYLAELSKNTPSAANIS
HCCCHHHHHHHHHHHHHCCCCEEEEEEHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCHHH
AYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDLAESRVFKIAESRANKDEGPK
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHH
NIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKTAGLQPSDLIIVAARPSMGKTTF
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHCCHHCCCCCCCEEEEEECCCCCCHHH
AMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASLSRVDQTKIRTGQLDDEDWARISGT
HHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCC
MGILLEKRNIYIDDSSGLTPTEVRSRARRIAREHGGIGLIMIDYLQLMRVPALSDNRTLE
EEEEEEECEEEEECCCCCCHHHHHHHHHHHHHHHCCEEEHHHHHHHHHHCCCCCCCCEEE
IAEISRSLKALAKELNVPVVALSQLNRSLEQRADKRPVNSDLRESGSIEQDADLIMFIYR
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCCCEEEEEEE
DEVYHENSDLKGIAEIIIGKQRNGPIGTVRLTFNGQWSRFDNYAGPLYDDE
HHHHCCCCCHHHHHHHHHCCCCCCCEEEEEEEECCCCHHCCCCCCCCCCCC
>Mature Secondary Structure 
AGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLDNERWDDVAERVVADDF
CCCCCCCCCCCCCCCCCCCCEEEECCCCCCCHHHHHHCCCEECCCHHHHHHHHHHHHHH
YTRPHRHIFTEMARLQESGSPIDLITLTESLERQGQLDSVGGFAYLAELSKNTPSAANIS
HCCCHHHHHHHHHHHHHCCCCEEEEEEHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCHHH
AYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDLAESRVFKIAESRANKDEGPK
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHH
NIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKTAGLQPSDLIIVAARPSMGKTTF
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHCCHHCCCCCCCEEEEEECCCCCCHHH
AMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASLSRVDQTKIRTGQLDDEDWARISGT
HHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCC
MGILLEKRNIYIDDSSGLTPTEVRSRARRIAREHGGIGLIMIDYLQLMRVPALSDNRTLE
EEEEEEECEEEEECCCCCCHHHHHHHHHHHHHHHCCEEEHHHHHHHHHHCCCCCCCCEEE
IAEISRSLKALAKELNVPVVALSQLNRSLEQRADKRPVNSDLRESGSIEQDADLIMFIYR
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCCCEEEEEEE
DEVYHENSDLKGIAEIIIGKQRNGPIGTVRLTFNGQWSRFDNYAGPLYDDE
HHHHCCCCCHHHHHHHHHCCCCCCCEEEEEEEECCCCHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]