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The map label for this gene is hflK [C]

Identifier: 218702666

GI number: 218702666

Start: 4623266

End: 4624393

Strand: Direct

Name: hflK [C]

Synonym: ECIAI39_4422

Alternate gene names: 218702666

Gene position: 4623266-4624393 (Clockwise)

Preceding gene: 218702664

Following gene: 218702667

Centisome position: 90.09

GC content: 50.0

Gene sequence:

>1128_bases
ATGATTAAGAAAATAAGCGTAAGAAAAGATCAGCTGGCATTACTCTCCAGAAATGGCGATTACTACAAAGTGCTGCATGC
AGGCGAGCATCTTTTACCCTGGTTAAATACGCCAGAAGTCTTGCTGATTACGCTCGATGGCAGCGAAGTTCCCGACGTGC
TGGCGGATTATTTGCGTCGCTTTCAGCCAGAGTGGGTGGAAAGATATTGCGTGGTGGCCGACTTGTCGGAGACTGAAGCC
GGGGCGTTGTATATGGACGGTATTTTGCTGGAGATCCTGCCGCCATCAACGCGTCGTTTGTACTGGCGTGTAGAGGATGA
TTTGACGCTTGTGCGGATGAATACGCAGCAAGTCCAGGTTCAAACGGAGGTGATGAATGCGGTATTGCAGCCACGGCGCA
AAGGGGCAGTTAAAGGCCGCGATGCGATACTGACGGTGCAGGTTCCGGCCTGGCACGTAGGTGTACTGAAAATCGATGGT
AAAACGCAGGCGCTGTTACCGCCGGGACTGACTGCGTACTGGAAAATTAACCATCTGGTTGAAGCTGAAGTGGTTGACAC
CCGCTTGCAGGTACTGGAAGTCAGCGGGCAAGAAATCTTAACGAAAGATAAAGTCAATCTGCGGATTAACCTCGCCGCCA
ACTGGCGCTACAGCGACGTGTTATTGGCATTTAGTCAGTTAACGAAGCCGATTGATCATTTGTATCGGGAATTGCAATTT
GCACTGCGTGAAGCGGTAGGTACGCGTACGCTGGATGAATTGCTGGAAGATAAACAAGTCATTGATGACGTTGTCAGCGA
ACAGGTGAAATCGCGCATGTTGCCATTTGGTATGGAGATTGCTTCATTAGGGGTGAAAGATATCGTCCTGCCTGGTGATA
TGAAAAATATCCTCGCGCAGCTGGTTGAGGCAGAGAAATCTGCGCAGGCGAATGTGATTCGCCGGCGTGAAGAAACGGCA
GCGACGCGTTCGTTACTCAATACCGCTAAAGTCATGGAAAACAACCCGGTGGCGTTACGCTTAAAAGAACTGGAAACGCT
GGAGAGAGTGGCTGAGCGGATCGATAACATCTCTGTATTTGGTGGTCTGGATCAGGTTCTGCACGGGTTAGTGAATATCA
AAGGATGA

Upstream 100 bases:

>100_bases
CGATAACGTCGCCGATAAAGACGCCGGGTAACAGGCAAAAGGGAATTCCCCTGTCGCTTCGTATCTGCTTCCGCGACGGC
TGTTAAGCAAGGAATAGATA

Downstream 100 bases:

>100_bases
GTCAAATGCCATTAAATGGAGTCAGTGCTGCGATGCGCGAACGGGTATCGCAGCAGTTAAAAGAAATAGAACGTCGCTAT
GGCGTGAAAGTGCTGTACGC

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 375; Mature: 375

Protein sequence:

>375_residues
MIKKISVRKDQLALLSRNGDYYKVLHAGEHLLPWLNTPEVLLITLDGSEVPDVLADYLRRFQPEWVERYCVVADLSETEA
GALYMDGILLEILPPSTRRLYWRVEDDLTLVRMNTQQVQVQTEVMNAVLQPRRKGAVKGRDAILTVQVPAWHVGVLKIDG
KTQALLPPGLTAYWKINHLVEAEVVDTRLQVLEVSGQEILTKDKVNLRINLAANWRYSDVLLAFSQLTKPIDHLYRELQF
ALREAVGTRTLDELLEDKQVIDDVVSEQVKSRMLPFGMEIASLGVKDIVLPGDMKNILAQLVEAEKSAQANVIRRREETA
ATRSLLNTAKVMENNPVALRLKELETLERVAERIDNISVFGGLDQVLHGLVNIKG

Sequences:

>Translated_375_residues
MIKKISVRKDQLALLSRNGDYYKVLHAGEHLLPWLNTPEVLLITLDGSEVPDVLADYLRRFQPEWVERYCVVADLSETEA
GALYMDGILLEILPPSTRRLYWRVEDDLTLVRMNTQQVQVQTEVMNAVLQPRRKGAVKGRDAILTVQVPAWHVGVLKIDG
KTQALLPPGLTAYWKINHLVEAEVVDTRLQVLEVSGQEILTKDKVNLRINLAANWRYSDVLLAFSQLTKPIDHLYRELQF
ALREAVGTRTLDELLEDKQVIDDVVSEQVKSRMLPFGMEIASLGVKDIVLPGDMKNILAQLVEAEKSAQANVIRRREETA
ATRSLLNTAKVMENNPVALRLKELETLERVAERIDNISVFGGLDQVLHGLVNIKG
>Mature_375_residues
MIKKISVRKDQLALLSRNGDYYKVLHAGEHLLPWLNTPEVLLITLDGSEVPDVLADYLRRFQPEWVERYCVVADLSETEA
GALYMDGILLEILPPSTRRLYWRVEDDLTLVRMNTQQVQVQTEVMNAVLQPRRKGAVKGRDAILTVQVPAWHVGVLKIDG
KTQALLPPGLTAYWKINHLVEAEVVDTRLQVLEVSGQEILTKDKVNLRINLAANWRYSDVLLAFSQLTKPIDHLYRELQF
ALREAVGTRTLDELLEDKQVIDDVVSEQVKSRMLPFGMEIASLGVKDIVLPGDMKNILAQLVEAEKSAQANVIRRREETA
ATRSLLNTAKVMENNPVALRLKELETLERVAERIDNISVFGGLDQVLHGLVNIKG

Specific function: Hflc And Hflk Govern The Stability Of Phage Lambda Cii Protein And Have Been Proposed To Encode Or Regulate A Cii- Specific Protease. [C]

COG id: COG0330

COG function: function code O; Membrane protease subunits, stomatin/prohibitin homologs

Gene ontology:

Cell location: Membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the band 7/mec-2 family [H]

Homologues:

Organism=Homo sapiens, GI38016911, Length=191, Percent_Identity=29.8429319371728, Blast_Score=73, Evalue=4e-13,
Organism=Homo sapiens, GI7657615, Length=188, Percent_Identity=28.1914893617021, Blast_Score=69, Evalue=7e-12,
Organism=Homo sapiens, GI221316744, Length=186, Percent_Identity=26.8817204301075, Blast_Score=66, Evalue=7e-11,
Organism=Homo sapiens, GI21686995, Length=186, Percent_Identity=26.8817204301075, Blast_Score=66, Evalue=7e-11,
Organism=Caenorhabditis elegans, GI17569497, Length=177, Percent_Identity=29.3785310734463, Blast_Score=82, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI17570459, Length=208, Percent_Identity=25.9615384615385, Blast_Score=78, Evalue=7e-15,
Organism=Caenorhabditis elegans, GI71987621, Length=176, Percent_Identity=30.1136363636364, Blast_Score=75, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI25153583, Length=176, Percent_Identity=30.1136363636364, Blast_Score=74, Evalue=9e-14,
Organism=Caenorhabditis elegans, GI71987612, Length=176, Percent_Identity=30.1136363636364, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI193210507, Length=176, Percent_Identity=30.6818181818182, Blast_Score=74, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17570161, Length=176, Percent_Identity=30.6818181818182, Blast_Score=74, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17569499, Length=193, Percent_Identity=26.9430051813472, Blast_Score=72, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI32566490, Length=174, Percent_Identity=32.183908045977, Blast_Score=72, Evalue=7e-13,
Organism=Caenorhabditis elegans, GI17569493, Length=192, Percent_Identity=25, Blast_Score=65, Evalue=5e-11,
Organism=Caenorhabditis elegans, GI71989948, Length=203, Percent_Identity=26.1083743842365, Blast_Score=65, Evalue=7e-11,
Organism=Caenorhabditis elegans, GI193209764, Length=190, Percent_Identity=24.2105263157895, Blast_Score=65, Evalue=8e-11,
Organism=Drosophila melanogaster, GI28573263, Length=192, Percent_Identity=27.6041666666667, Blast_Score=79, Evalue=6e-15,
Organism=Drosophila melanogaster, GI21356845, Length=192, Percent_Identity=28.125, Blast_Score=75, Evalue=6e-14,
Organism=Drosophila melanogaster, GI281365664, Length=177, Percent_Identity=29.9435028248588, Blast_Score=73, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24657857, Length=177, Percent_Identity=29.9435028248588, Blast_Score=73, Evalue=3e-13,
Organism=Drosophila melanogaster, GI45556022, Length=191, Percent_Identity=27.2251308900524, Blast_Score=73, Evalue=4e-13,
Organism=Drosophila melanogaster, GI62484448, Length=177, Percent_Identity=29.9435028248588, Blast_Score=72, Evalue=5e-13,
Organism=Drosophila melanogaster, GI62484274, Length=177, Percent_Identity=29.9435028248588, Blast_Score=72, Evalue=7e-13,
Organism=Drosophila melanogaster, GI281361631, Length=211, Percent_Identity=27.4881516587678, Blast_Score=71, Evalue=1e-12,
Organism=Drosophila melanogaster, GI281361633, Length=211, Percent_Identity=27.4881516587678, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI18860517, Length=196, Percent_Identity=29.0816326530612, Blast_Score=69, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001107
- InterPro:   IPR018080
- InterPro:   IPR001972 [H]

Pfam domain/function: PF01145 Band_7 [H]

EC number: NA

Molecular weight: Translated: 42362; Mature: 42362

Theoretical pI: Translated: 5.76; Mature: 5.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKKISVRKDQLALLSRNGDYYKVLHAGEHLLPWLNTPEVLLITLDGSEVPDVLADYLRR
CCCCCCCCHHHHHHEECCCCEEEEEECCCHHCCCCCCCCEEEEEECCCCCHHHHHHHHHH
FQPEWVERYCVVADLSETEAGALYMDGILLEILPPSTRRLYWRVEDDLTLVRMNTQQVQV
CCHHHHHHEEEEEECCCCCCCEEEECCEEEEECCCCCCEEEEEEECCEEEEEECCHHHHH
QTEVMNAVLQPRRKGAVKGRDAILTVQVPAWHVGVLKIDGKTQALLPPGLTAYWKINHLV
HHHHHHHHHCHHHCCCCCCCCEEEEEEECCEEEEEEEECCCCCEECCCCCEEEEEECHHH
EAEVVDTRLQVLEVSGQEILTKDKVNLRINLAANWRYSDVLLAFSQLTKPIDHLYRELQF
HHHHHHHHHHHHCCCCCHHEECCCEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHH
ALREAVGTRTLDELLEDKQVIDDVVSEQVKSRMLPFGMEIASLGVKDIVLPGDMKNILAQ
HHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCEEECCCCHHHHHHH
LVEAEKSAQANVIRRREETAATRSLLNTAKVMENNPVALRLKELETLERVAERIDNISVF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHCCEEEE
GGLDQVLHGLVNIKG
CCHHHHHHHHHCCCC
>Mature Secondary Structure
MIKKISVRKDQLALLSRNGDYYKVLHAGEHLLPWLNTPEVLLITLDGSEVPDVLADYLRR
CCCCCCCCHHHHHHEECCCCEEEEEECCCHHCCCCCCCCEEEEEECCCCCHHHHHHHHHH
FQPEWVERYCVVADLSETEAGALYMDGILLEILPPSTRRLYWRVEDDLTLVRMNTQQVQV
CCHHHHHHEEEEEECCCCCCCEEEECCEEEEECCCCCCEEEEEEECCEEEEEECCHHHHH
QTEVMNAVLQPRRKGAVKGRDAILTVQVPAWHVGVLKIDGKTQALLPPGLTAYWKINHLV
HHHHHHHHHCHHHCCCCCCCCEEEEEEECCEEEEEEEECCCCCEECCCCCEEEEEECHHH
EAEVVDTRLQVLEVSGQEILTKDKVNLRINLAANWRYSDVLLAFSQLTKPIDHLYRELQF
HHHHHHHHHHHHCCCCCHHEECCCEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHH
ALREAVGTRTLDELLEDKQVIDDVVSEQVKSRMLPFGMEIASLGVKDIVLPGDMKNILAQ
HHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCEEECCCCHHHHHHH
LVEAEKSAQANVIRRREETAATRSLLNTAKVMENNPVALRLKELETLERVAERIDNISVF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHCCEEEE
GGLDQVLHGLVNIKG
CCHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on peptide bonds (Peptidases) [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12622808 [H]