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The map label for this gene is b0257 [C]

Identifier: 218702445

GI number: 218702445

Start: 4372538

End: 4373020

Strand: Direct

Name: b0257 [C]

Synonym: ECIAI39_4198

Alternate gene names: 218702445

Gene position: 4372538-4373020 (Clockwise)

Preceding gene: 218702444

Following gene: 218702446

Centisome position: 85.2

GC content: 54.24

Gene sequence:

>483_bases
GTGATGGCACTGCTGGATAAGCTGCGTGAGCAGTACGGGGTCAGACCGGTATGCAGTGAACTGCATATTGCCCCGTCAAC
GTATTACCACTGTCAGCAACAGCGACATCATCCTGATAAACGCAGTGCACATGCACAGCGCGATGACTGGATGAAGAGAG
AGATACAGCGCGTATACGATGAAAATCATCAGGTGTACGGTGTGCGTAAAGTCTGGCGTCAGTTGTTACGGGAAGGAATC
AGGGTAGCCAGATGTACAGTGGCGCGCCTCATGGCGGTTATGGGACTTGCCGGTGTTCTCCGGGGTAAAAAGGTCCGCAC
TACCGTCAGCCGGAAAGCCGTTGCCGCAGGCGACCGCGTAAACCGTCAGTTTGTGGCAGAACGTCCTGACCAGTTGTGGG
TGGCTGATTTTACTTACGGTGTGCCCGGAGTTCAGGGCGGGCATGGATGCTTAAATGAACCGCGAGTCTGTCTGGAATAT
TGA

Upstream 100 bases:

>100_bases
AGCTGGAACGTGAAAATCGTGAACTGCGCCGCAGTAACAATATCCTTCGCCAGGCTTCCGCTTATTTTGCGAAGGCGGAG
TTCGACCGCCTCTGGAAAAA

Downstream 100 bases:

>100_bases
ACCGGTAACTCACGATGAGAAACCCAACAATCCCACCGGGTGTGACGGTGGAGAACCTGAGCGGCAGTGACCTGCGGCAT
GCCCGCAGGGTGATGTAACC

Product: transposase ORF B (fragment), IS629

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 160; Mature: 160

Protein sequence:

>160_residues
MMALLDKLREQYGVRPVCSELHIAPSTYYHCQQQRHHPDKRSAHAQRDDWMKREIQRVYDENHQVYGVRKVWRQLLREGI
RVARCTVARLMAVMGLAGVLRGKKVRTTVSRKAVAAGDRVNRQFVAERPDQLWVADFTYGVPGVQGGHGCLNEPRVCLEY

Sequences:

>Translated_160_residues
MMALLDKLREQYGVRPVCSELHIAPSTYYHCQQQRHHPDKRSAHAQRDDWMKREIQRVYDENHQVYGVRKVWRQLLREGI
RVARCTVARLMAVMGLAGVLRGKKVRTTVSRKAVAAGDRVNRQFVAERPDQLWVADFTYGVPGVQGGHGCLNEPRVCLEY
>Mature_160_residues
MMALLDKLREQYGVRPVCSELHIAPSTYYHCQQQRHHPDKRSAHAQRDDWMKREIQRVYDENHQVYGVRKVWRQLLREGI
RVARCTVARLMAVMGLAGVLRGKKVRTTVSRKAVAAGDRVNRQFVAERPDQLWVADFTYGVPGVQGGHGCLNEPRVCLEY

Specific function: Involved in the transposition of the insertion sequence [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 integrase catalytic domain [H]

Homologues:

Organism=Homo sapiens, GI310129419, Length=140, Percent_Identity=45, Blast_Score=122, Evalue=1e-28,
Organism=Escherichia coli, GI1789981, Length=143, Percent_Identity=30.7692307692308, Blast_Score=69, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001584
- InterPro:   IPR012337 [H]

Pfam domain/function: PF00665 rve [H]

EC number: NA

Molecular weight: Translated: 18498; Mature: 18498

Theoretical pI: Translated: 10.16; Mature: 10.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.1 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
3.1 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMALLDKLREQYGVRPVCSELHIAPSTYYHCQQQRHHPDKRSAHAQRDDWMKREIQRVYD
CHHHHHHHHHHHCCCHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
ENHQVYGVRKVWRQLLREGIRVARCTVARLMAVMGLAGVLRGKKVRTTVSRKAVAAGDRV
CCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCHHH
NRQFVAERPDQLWVADFTYGVPGVQGGHGCLNEPRVCLEY
HHHHHHCCCCCEEEEHHCCCCCCCCCCCCCCCCCCEECCC
>Mature Secondary Structure
MMALLDKLREQYGVRPVCSELHIAPSTYYHCQQQRHHPDKRSAHAQRDDWMKREIQRVYD
CHHHHHHHHHHHCCCHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
ENHQVYGVRKVWRQLLREGIRVARCTVARLMAVMGLAGVLRGKKVRTTVSRKAVAAGDRV
CCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCHHH
NRQFVAERPDQLWVADFTYGVPGVQGGHGCLNEPRVCLEY
HHHHHHCCCCCEEEEHHCCCCCCCCCCCCCCCCCCEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2159625; 2824781 [H]