Click here to switch to the map view.

The map label for this gene is malS

Identifier: 218702337

GI number: 218702337

Start: 4252520

End: 4254550

Strand: Direct

Name: malS

Synonym: ECIAI39_4083

Alternate gene names: 218702337

Gene position: 4252520-4254550 (Clockwise)

Preceding gene: 218702335

Following gene: 218702338

Centisome position: 82.86

GC content: 52.93

Gene sequence:

>2031_bases
ATGAAACTCGCCGCCTGTTTTCTGACACTCCTTCCTGGCTTCGCCGTTGCCGCCAGCTGGACTTCTCCGGGGTTTCCCAC
CTTTAGCGAACAGGGGACGGGAACATTTGTCAGCCACGCGCAGTTGCCCAAAGGTACGCGTCCACTAACGCTAAATTTTG
ACCAACAGTGCTGGCAGCCTGCGGATGCGATAAAACTCAATCAGATGCTTTCCCTGCAACCTTGTAGCAACACGCCGCCT
CAATGGCGATTGTTCAGGGACGGCGAATATACGCTGCAACTAGACACCCGCTCCGGTACACCAACATTGATGATTTCCAT
CCAGAACGCCGTCGAACCGGTAGCAAGCCTGGTCCGTGAATGCCCGAAATGGGATGGATTACCGCTCACACTGGATGTCA
GCGCCACTTTTCCGGAGGGAGCCGCCGTCCGGGATTATTACAGCCAGCAAATTGCGATAGTGAAGAACGGTCAAATAACG
TTACAACCCGCTGCCACCAGCAACGGTTTACTCCTGCTGGAACGGGCAGAAACTGACGCCTCTGCCCCTTTCGACTGGCA
TAACGCCACGGTTTACTTTGTGCTGACAGATCGTTTCGAAAACGGCGATCCCAGTAATGACCAGAGTTACGGACGTCATA
AAGACGGTATGGCAGAAATTGGCACTTTTCACGGCGGCGATTTACGCGGCCTGACCAACAAACTGGATTACCTCCAGCAG
CTGGGCGTCAATGCTTTATGGATAAGTGCCCCATTTGAGCAAATTCACGGCTGGGTCGGCGGCGGTACAAAAGGCGATTT
CCCGCATTATGCCTACCACGGTTATTACACACAGGACTGGACGAATCTTGATGCCAATATGGGCCGCGAAGCCGATCTAC
GGACACTGGTTGATAGCGCGCATCAGCGCGGTATTCGTATTCTCTTTGATGTCGTGATGAACCACACCGGTTATGCCACA
CTGGCGGATATGCAGGAGTATCAGTTTGGCGCGTTATATCTTTCTGGTGACGAGCTGAAAAAAACGTTGGGTGAACGCTG
GAGCGACTGGAAACCTGCCGCCGGGCAAACCTGGCATAGCTTTAACGATTACATTAATTTCAGCGACAAAACAGGCTGGG
ATAAATGGTGGGGAAAAAACTGGATCAGAACCGATATCGGCGATTACGACAATCCTGGATTCGACGATCTCACTATGTCG
CTGGCCTTTTTGCCGGATATCAAAACCGAATCAACGACCGCTTCTGGTCTGCCGGTGTTCTATAAAAACAAAACGGATAC
CCACGCTAAAGTCATCGACGGCTTTACCCCTCGCGATTACTTAACCCACTGGTTAAGTCAGTGGGTTCGCGACTATGGGA
TTGATGGTTTTCGGGTCGACACCGCCAAACATGTTGAGTTGCCCGCCTGGCAGCAACTGAAAACCGAAGCCAGCGCCGCG
CTTCGCGAATGGAAAAAAGCTAACTCCGACAAAGCATTAGATGACAAGCCTTTCTGGATGACCGGTGAAGCCTGGGGCCA
CGGCGTGATGCAAAGTGACTACTATCGCCACGGCTTCGATGCGATGATCAATTTCGATTATCAGGAGCAGGCGGCGAAAG
CAGTCGATTGTTTTGCGCAGATGGATACGACCTGGCAGCAAATGGCGGAGAAATTGCAGGATTTCAACGTGTTGAGCTAC
CTCTCGTCGCATGATACCCGCCTGTTCCGCGAAGGTGGTAATAAAGCAGCAGAGTTATTGTTATTAGCGCCAGGCGCGGT
ACAAATCTTTTATGGTGATGAATCCTCGCGTCCGTTCGGCCCCACCGGTTCGGATCCACTGCAAGGCACGCGTTCGGATA
TGAACTGGCAGGATGTTAGCGGCAAATCTGCCGCCAGCGTCGCGCACTGGCAGAAAATCAGCCAGTTCCGCGCCCGCCAT
CCCGCAATTGGCGAGGGCAAACAAACGACACTTTCGCTGAAGCAGGGCTACGGCTTTGTTCGTGAGCATGGCGACGATAA
AGTGCTGGTCATCTGGGCTGGGCAACAGTGA

Upstream 100 bases:

>100_bases
CCTTGCTTCTCATCCTGTGGACTTACCGCTCAGGGATGAGTTTTGTCTGGCTTATCGCTGGCAAACTGTCTGAAATCGCA
GCAATAAGGACTCATTCGCC

Downstream 100 bases:

>100_bases
CTTTTCCGGCTTCCCGTTCGTCAGTACCTCGGGAAGCCGCCAACCAGGATAAAATGTCAGCTCTAATCAGCGTTGCAGGA
TAAAGCACTGCTCCCTCTTC

Product: periplasmic alpha-amylase

Products: NA

Alternate protein names: 1,4-alpha-D-glucan glucanohydrolase [H]

Number of amino acids: Translated: 676; Mature: 676

Protein sequence:

>676_residues
MKLAACFLTLLPGFAVAASWTSPGFPTFSEQGTGTFVSHAQLPKGTRPLTLNFDQQCWQPADAIKLNQMLSLQPCSNTPP
QWRLFRDGEYTLQLDTRSGTPTLMISIQNAVEPVASLVRECPKWDGLPLTLDVSATFPEGAAVRDYYSQQIAIVKNGQIT
LQPAATSNGLLLLERAETDASAPFDWHNATVYFVLTDRFENGDPSNDQSYGRHKDGMAEIGTFHGGDLRGLTNKLDYLQQ
LGVNALWISAPFEQIHGWVGGGTKGDFPHYAYHGYYTQDWTNLDANMGREADLRTLVDSAHQRGIRILFDVVMNHTGYAT
LADMQEYQFGALYLSGDELKKTLGERWSDWKPAAGQTWHSFNDYINFSDKTGWDKWWGKNWIRTDIGDYDNPGFDDLTMS
LAFLPDIKTESTTASGLPVFYKNKTDTHAKVIDGFTPRDYLTHWLSQWVRDYGIDGFRVDTAKHVELPAWQQLKTEASAA
LREWKKANSDKALDDKPFWMTGEAWGHGVMQSDYYRHGFDAMINFDYQEQAAKAVDCFAQMDTTWQQMAEKLQDFNVLSY
LSSHDTRLFREGGNKAAELLLLAPGAVQIFYGDESSRPFGPTGSDPLQGTRSDMNWQDVSGKSAASVAHWQKISQFRARH
PAIGEGKQTTLSLKQGYGFVREHGDDKVLVIWAGQQ

Sequences:

>Translated_676_residues
MKLAACFLTLLPGFAVAASWTSPGFPTFSEQGTGTFVSHAQLPKGTRPLTLNFDQQCWQPADAIKLNQMLSLQPCSNTPP
QWRLFRDGEYTLQLDTRSGTPTLMISIQNAVEPVASLVRECPKWDGLPLTLDVSATFPEGAAVRDYYSQQIAIVKNGQIT
LQPAATSNGLLLLERAETDASAPFDWHNATVYFVLTDRFENGDPSNDQSYGRHKDGMAEIGTFHGGDLRGLTNKLDYLQQ
LGVNALWISAPFEQIHGWVGGGTKGDFPHYAYHGYYTQDWTNLDANMGREADLRTLVDSAHQRGIRILFDVVMNHTGYAT
LADMQEYQFGALYLSGDELKKTLGERWSDWKPAAGQTWHSFNDYINFSDKTGWDKWWGKNWIRTDIGDYDNPGFDDLTMS
LAFLPDIKTESTTASGLPVFYKNKTDTHAKVIDGFTPRDYLTHWLSQWVRDYGIDGFRVDTAKHVELPAWQQLKTEASAA
LREWKKANSDKALDDKPFWMTGEAWGHGVMQSDYYRHGFDAMINFDYQEQAAKAVDCFAQMDTTWQQMAEKLQDFNVLSY
LSSHDTRLFREGGNKAAELLLLAPGAVQIFYGDESSRPFGPTGSDPLQGTRSDMNWQDVSGKSAASVAHWQKISQFRARH
PAIGEGKQTTLSLKQGYGFVREHGDDKVLVIWAGQQ
>Mature_676_residues
MKLAACFLTLLPGFAVAASWTSPGFPTFSEQGTGTFVSHAQLPKGTRPLTLNFDQQCWQPADAIKLNQMLSLQPCSNTPP
QWRLFRDGEYTLQLDTRSGTPTLMISIQNAVEPVASLVRECPKWDGLPLTLDVSATFPEGAAVRDYYSQQIAIVKNGQIT
LQPAATSNGLLLLERAETDASAPFDWHNATVYFVLTDRFENGDPSNDQSYGRHKDGMAEIGTFHGGDLRGLTNKLDYLQQ
LGVNALWISAPFEQIHGWVGGGTKGDFPHYAYHGYYTQDWTNLDANMGREADLRTLVDSAHQRGIRILFDVVMNHTGYAT
LADMQEYQFGALYLSGDELKKTLGERWSDWKPAAGQTWHSFNDYINFSDKTGWDKWWGKNWIRTDIGDYDNPGFDDLTMS
LAFLPDIKTESTTASGLPVFYKNKTDTHAKVIDGFTPRDYLTHWLSQWVRDYGIDGFRVDTAKHVELPAWQQLKTEASAA
LREWKKANSDKALDDKPFWMTGEAWGHGVMQSDYYRHGFDAMINFDYQEQAAKAVDCFAQMDTTWQQMAEKLQDFNVLSY
LSSHDTRLFREGGNKAAELLLLAPGAVQIFYGDESSRPFGPTGSDPLQGTRSDMNWQDVSGKSAASVAHWQKISQFRARH
PAIGEGKQTTLSLKQGYGFVREHGDDKVLVIWAGQQ

Specific function: Since only maltooligosaccharides up to a chain length of 6 glucose units are actively transported through the cytoplasmic membrane via the membrane-bound complex of three proteins, MalF, MalG, and MalK, longer maltooligosaccharides must first be degraded

COG id: COG0366

COG function: function code G; Glycosidases

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Escherichia coli, GI1789995, Length=676, Percent_Identity=97.189349112426, Blast_Score=1371, Evalue=0.0,
Organism=Escherichia coli, GI1786604, Length=143, Percent_Identity=30.0699300699301, Blast_Score=63, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014635
- InterPro:   IPR006047
- InterPro:   IPR006589
- InterPro:   IPR017853
- InterPro:   IPR013781 [H]

Pfam domain/function: PF00128 Alpha-amylase [H]

EC number: =3.2.1.1 [H]

Molecular weight: Translated: 75904; Mature: 75904

Theoretical pI: Translated: 5.35; Mature: 5.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLAACFLTLLPGFAVAASWTSPGFPTFSEQGTGTFVSHAQLPKGTRPLTLNFDQQCWQP
CCHHHHHHHHHCCHHEEECCCCCCCCCCCCCCCCCEEECCCCCCCCCCEEECCCHHHCCC
ADAIKLNQMLSLQPCSNTPPQWRLFRDGEYTLQLDTRSGTPTLMISIQNAVEPVASLVRE
HHHHHHHHHEEECCCCCCCCCEEEEECCCEEEEEECCCCCCEEEEEEHHHHHHHHHHHHH
CPKWDGLPLTLDVSATFPEGAAVRDYYSQQIAIVKNGQITLQPAATSNGLLLLERAETDA
CCCCCCCEEEEEECCCCCCCCHHHHHHCCEEEEEECCEEEEEECCCCCCEEEEEECCCCC
SAPFDWHNATVYFVLTDRFENGDPSNDQSYGRHKDGMAEIGTFHGGDLRGLTNKLDYLQQ
CCCCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCHHHHCCCCCCCHHHHHHHHHHHHH
LGVNALWISAPFEQIHGWVGGGTKGDFPHYAYHGYYTQDWTNLDANMGREADLRTLVDSA
CCCCEEEEECCHHHHCCCCCCCCCCCCCCEEECCEEECCCCCCCCCCCCCHHHHHHHHHH
HQRGIRILFDVVMNHTGYATLADMQEYQFGALYLSGDELKKTLGERWSDWKPAAGQTWHS
HHCCHHHHHHHHHCCCCCEEHHHHHHCCEEEEEECHHHHHHHHHHCCCCCCCCCCCCCCC
FNDYINFSDKTGWDKWWGKNWIRTDIGDYDNPGFDDLTMSLAFLPDIKTESTTASGLPVF
HHHEECCCCCCCCHHHHCCCCEECCCCCCCCCCCHHHHEEEEECCCCCCCCCCCCCCEEE
YKNKTDTHAKVIDGFTPRDYLTHWLSQWVRDYGIDGFRVDTAKHVELPAWQQLKTEASAA
ECCCCCCCHHHHCCCCCHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCHHHHHHHHHHH
LREWKKANSDKALDDKPFWMTGEAWGHGVMQSDYYRHGFDAMINFDYQEQAAKAVDCFAQ
HHHHHHCCCCCCCCCCCCEEECHHHCCCCCCCCCHHCCCCEEEECCHHHHHHHHHHHHHH
MDTTWQQMAEKLQDFNVLSYLSSHDTRLFREGGNKAAELLLLAPGAVQIFYGDESSRPFG
HCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCEEEEEEECCCEEEEEECCCCCCCCC
PTGSDPLQGTRSDMNWQDVSGKSAASVAHWQKISQFRARHPAIGEGKQTTLSLKQGYGFV
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCEEEEHHHCCCHH
REHGDDKVLVIWAGQQ
HCCCCCEEEEEECCCC
>Mature Secondary Structure
MKLAACFLTLLPGFAVAASWTSPGFPTFSEQGTGTFVSHAQLPKGTRPLTLNFDQQCWQP
CCHHHHHHHHHCCHHEEECCCCCCCCCCCCCCCCCEEECCCCCCCCCCEEECCCHHHCCC
ADAIKLNQMLSLQPCSNTPPQWRLFRDGEYTLQLDTRSGTPTLMISIQNAVEPVASLVRE
HHHHHHHHHEEECCCCCCCCCEEEEECCCEEEEEECCCCCCEEEEEEHHHHHHHHHHHHH
CPKWDGLPLTLDVSATFPEGAAVRDYYSQQIAIVKNGQITLQPAATSNGLLLLERAETDA
CCCCCCCEEEEEECCCCCCCCHHHHHHCCEEEEEECCEEEEEECCCCCCEEEEEECCCCC
SAPFDWHNATVYFVLTDRFENGDPSNDQSYGRHKDGMAEIGTFHGGDLRGLTNKLDYLQQ
CCCCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCHHHHCCCCCCCHHHHHHHHHHHHH
LGVNALWISAPFEQIHGWVGGGTKGDFPHYAYHGYYTQDWTNLDANMGREADLRTLVDSA
CCCCEEEEECCHHHHCCCCCCCCCCCCCCEEECCEEECCCCCCCCCCCCCHHHHHHHHHH
HQRGIRILFDVVMNHTGYATLADMQEYQFGALYLSGDELKKTLGERWSDWKPAAGQTWHS
HHCCHHHHHHHHHCCCCCEEHHHHHHCCEEEEEECHHHHHHHHHHCCCCCCCCCCCCCCC
FNDYINFSDKTGWDKWWGKNWIRTDIGDYDNPGFDDLTMSLAFLPDIKTESTTASGLPVF
HHHEECCCCCCCCHHHHCCCCEECCCCCCCCCCCHHHHEEEEECCCCCCCCCCCCCCEEE
YKNKTDTHAKVIDGFTPRDYLTHWLSQWVRDYGIDGFRVDTAKHVELPAWQQLKTEASAA
ECCCCCCCHHHHCCCCCHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCHHHHHHHHHHH
LREWKKANSDKALDDKPFWMTGEAWGHGVMQSDYYRHGFDAMINFDYQEQAAKAVDCFAQ
HHHHHHCCCCCCCCCCCCEEECHHHCCCCCCCCCHHCCCCEEEECCHHHHHHHHHHHHHH
MDTTWQQMAEKLQDFNVLSYLSSHDTRLFREGGNKAAELLLLAPGAVQIFYGDESSRPFG
HCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCEEEEEEECCCEEEEEECCCCCCCCC
PTGSDPLQGTRSDMNWQDVSGKSAASVAHWQKISQFRARHPAIGEGKQTTLSLKQGYGFV
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCEEEEHHHCCCHH
REHGDDKVLVIWAGQQ
HCCCCCEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1544897; 8041620; 9278503; 9268356 [H]