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The map label for this gene is xylF

Identifier: 218702332

GI number: 218702332

Start: 4246242

End: 4247234

Strand: Direct

Name: xylF

Synonym: ECIAI39_4078

Alternate gene names: 218702332

Gene position: 4246242-4247234 (Clockwise)

Preceding gene: 218702321

Following gene: 218702333

Centisome position: 82.74

GC content: 43.61

Gene sequence:

>993_bases
ATGAAAATAAAGAACATTCTACTCACCCTTTGCACCTCACTTCTGCTTACCAACGTTGCGGCTCACGCCAAAGAAGTCAA
AATAGGTATGGCGATTGATGATCTCCGTCTTGAACGCTGGCAAAAAGATCGAGATATTTTTGTGAAAAAGGCAGAATCTC
TCGGCGCGAAAGTATTTGTACAGTCTGCAAATGGCAATGAAGAAACACAAATGTCGCAGATTGAAAACATGATTAACCGG
GGTGTCGATGTTCTTGTCATTATTCCTTATAACGGTCAGGTATTAAGTAACGTTGTAAAAGAAGCCAAACAAGAAGGTAT
TAAAGTATTAGCTTACGACCGTATGATTAACGATGCGGATATCGATTTTTATATTTCTTTCGATAACGAAAAAGTCGGTG
AACTGCAGGCAAAAGCCCTGGTCGATATTGTTCCGCAAGGTAATTACTTCCTGATGGGCGGTTCGCCGGTAGATAACAAC
GCTAAGCTGTTCCGCGCCGGACAAATGAAAGTGTTAAAACCTTACGTTGATTCCGGAAAAATTAAAGTCGTTGGTGACCA
ATGGGTTGATGGCTGGTTACCGGAAAACGCACTGAAAATTATGGAAAACGCGCTGACCGCCAATAATAACAAAATTGATG
CTGTAGTTGCCTCAAACGATGCCACCGCAGGTGGGGCAATTCAGGCATTAAGCGCGCAAGGTTTATCAGGGAAAGTAGCA
ATTTCCGGCCAGGATGCGGATCTTGCAGGTATTAAACGTATTGCTGCCGGTACGCAAACTATGACGGTGTATAAACCTAT
TACATTGTTGGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATGGTCAGGAGCCAAAAGCGGATACCACACTGA
ATAATGGCCTGAAAGATGTTCCCTCCCGCCTGCTGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAGTT
AAAGACGGATTCCACAAAGAGAGCGAGCTGTAA

Upstream 100 bases:

>100_bases
GTAATTGTTTTCCCCTGTTTAGTTGCTAAAAATTGGTTACGTTTATCGCGGTGATTGTTACTTATTAAAGCTGTCCTCTA
ACTACAGAAGGCCCTACACC

Downstream 100 bases:

>100_bases
GCGTTACGCCCCGGCGCGGAGCGGGGGCGTGATTTCTCTCCATGCCGCGTGAATGAATTGGCTTAGGTGGAGTCGTTATG
CCTTATCTACTTGAAATGAA

Product: D-xylose transporter subunit XylF

Products: ADP; phosphate; xylose [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 330; Mature: 330

Protein sequence:

>330_residues
MKIKNILLTLCTSLLLTNVAAHAKEVKIGMAIDDLRLERWQKDRDIFVKKAESLGAKVFVQSANGNEETQMSQIENMINR
GVDVLVIIPYNGQVLSNVVKEAKQEGIKVLAYDRMINDADIDFYISFDNEKVGELQAKALVDIVPQGNYFLMGGSPVDNN
AKLFRAGQMKVLKPYVDSGKIKVVGDQWVDGWLPENALKIMENALTANNNKIDAVVASNDATAGGAIQALSAQGLSGKVA
ISGQDADLAGIKRIAAGTQTMTVYKPITLLANTAAEIAVELGNGQEPKADTTLNNGLKDVPSRLLTPIDVNKNNIKDTVV
KDGFHKESEL

Sequences:

>Translated_330_residues
MKIKNILLTLCTSLLLTNVAAHAKEVKIGMAIDDLRLERWQKDRDIFVKKAESLGAKVFVQSANGNEETQMSQIENMINR
GVDVLVIIPYNGQVLSNVVKEAKQEGIKVLAYDRMINDADIDFYISFDNEKVGELQAKALVDIVPQGNYFLMGGSPVDNN
AKLFRAGQMKVLKPYVDSGKIKVVGDQWVDGWLPENALKIMENALTANNNKIDAVVASNDATAGGAIQALSAQGLSGKVA
ISGQDADLAGIKRIAAGTQTMTVYKPITLLANTAAEIAVELGNGQEPKADTTLNNGLKDVPSRLLTPIDVNKNNIKDTVV
KDGFHKESEL
>Mature_330_residues
MKIKNILLTLCTSLLLTNVAAHAKEVKIGMAIDDLRLERWQKDRDIFVKKAESLGAKVFVQSANGNEETQMSQIENMINR
GVDVLVIIPYNGQVLSNVVKEAKQEGIKVLAYDRMINDADIDFYISFDNEKVGELQAKALVDIVPQGNYFLMGGSPVDNN
AKLFRAGQMKVLKPYVDSGKIKVVGDQWVDGWLPENALKIMENALTANNNKIDAVVASNDATAGGAIQALSAQGLSGKVA
ISGQDADLAGIKRIAAGTQTMTVYKPITLLANTAAEIAVELGNGQEPKADTTLNNGLKDVPSRLLTPIDVNKNNIKDTVV
KDGFHKESEL

Specific function: Involved in the high-affinity D-xylose membrane transport system. Binds with high affinity to xylose [H]

COG id: COG4213

COG function: function code G; ABC-type xylose transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 2 family [H]

Homologues:

Organism=Escherichia coli, GI1789990, Length=330, Percent_Identity=99.6969696969697, Blast_Score=664, Evalue=0.0,

Paralogues:

None

Copy number: 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001761
- InterPro:   IPR013456 [H]

Pfam domain/function: PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 35720; Mature: 35720

Theoretical pI: Translated: 5.26; Mature: 5.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIKNILLTLCTSLLLTNVAAHAKEVKIGMAIDDLRLERWQKDRDIFVKKAESLGAKVFV
CCHHHHHHHHHHHHHHHHHHHHHHHEEEEEEHHHHHHHHHCCCHHHHHHHHHCCCEEEEE
QSANGNEETQMSQIENMINRGVDVLVIIPYNGQVLSNVVKEAKQEGIKVLAYDRMINDAD
ECCCCCCHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHCCCEEEEEECCCCCCC
IDFYISFDNEKVGELQAKALVDIVPQGNYFLMGGSPVDNNAKLFRAGQMKVLKPYVDSGK
EEEEEEECCCCHHHHHHHHHEEEECCCCEEEECCCCCCCCCEEEECCCEEEECCCCCCCC
IKVVGDQWVDGWLPENALKIMENALTANNNKIDAVVASNDATAGGAIQALSAQGLSGKVA
EEEEECCCCCCCCCHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCEEEECCCCCCCEEE
ISGQDADLAGIKRIAAGTQTMTVYKPITLLANTAAEIAVELGNGQEPKADTTLNNGLKDV
ECCCCCCHHHHHHHHCCCCEEEEECHHHHHHCCHHHEEEECCCCCCCCCCCHHHCCHHHH
PSRLLTPIDVNKNNIKDTVVKDGFHKESEL
HHHHCCCCCCCCCCCHHHHHCCCCCCCCCC
>Mature Secondary Structure
MKIKNILLTLCTSLLLTNVAAHAKEVKIGMAIDDLRLERWQKDRDIFVKKAESLGAKVFV
CCHHHHHHHHHHHHHHHHHHHHHHHEEEEEEHHHHHHHHHCCCHHHHHHHHHCCCEEEEE
QSANGNEETQMSQIENMINRGVDVLVIIPYNGQVLSNVVKEAKQEGIKVLAYDRMINDAD
ECCCCCCHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHCCCEEEEEECCCCCCC
IDFYISFDNEKVGELQAKALVDIVPQGNYFLMGGSPVDNNAKLFRAGQMKVLKPYVDSGK
EEEEEEECCCCHHHHHHHHHEEEECCCCEEEECCCCCCCCCEEEECCCEEEECCCCCCCC
IKVVGDQWVDGWLPENALKIMENALTANNNKIDAVVASNDATAGGAIQALSAQGLSGKVA
EEEEECCCCCCCCCHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCEEEECCCCCCCEEE
ISGQDADLAGIKRIAAGTQTMTVYKPITLLANTAAEIAVELGNGQEPKADTTLNNGLKDV
ECCCCCCHHHHHHHHCCCCEEEEECHHHHHHCCHHHEEEECCCCCCCCCCCHHHCCHHHH
PSRLLTPIDVNKNNIKDTVVKDGFHKESEL
HHHHCCCCCCCCCCCHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; xylose [Periplasm]; H2O [C]

Specific reaction: ATP + xylose [Periplasm] + H2O = ADP + phosphate + xylose [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8041620; 9278503; 8581399; 9298646 [H]