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The map label for this gene is gadE

Identifier: 218702277

GI number: 218702277

Start: 4169695

End: 4170222

Strand: Direct

Name: gadE

Synonym: ECIAI39_4014

Alternate gene names: 218702277

Gene position: 4169695-4170222 (Clockwise)

Preceding gene: 218702276

Following gene: 218702278

Centisome position: 81.25

GC content: 33.14

Gene sequence:

>528_bases
ATGATTTTTCTCATGACGAAAGATTCTTTTCTTTTACAGGGCTTTTGGCAGTTGAAAGATAATCACGAAATGATAAAAAT
CAATTCCCTGTCAGAGATCAAAAAAGTAGGCAATAAACCCTTCAAGGTTATCATTGATACCTATCACAATCATATCCTTG
ATGAAGAAGCGATTAAATTTCTGGAGAAATTAGATGCCGAGAGAATTATTGTTTTGGCACCTTATCACATCAGTAAACTA
AAAGCTAAAGCGCCTATTTATTTTGTCAGCCGTAAAGAAAGTATCAAAAATCTTCTTGAGATTACTTATGGTAAACACTT
GCCCCATAAGAATTCACAACTATGTTTTTCACATAATCAGTTCAAAATTATGCAACTGATTCTGAAAAACAAAAATGAAA
GCAATATCACGTCAACGCTCAACATTTCGCAACAAACATTAAAGATTCAGAAATTCAACATTATGTACAAGCTGAAATTA
AGACGTATGAGCGACATCGTCACCCTGGGTATCACATCTTATTTTTAG

Upstream 100 bases:

>100_bases
GTTCTTATAGGCGTTTACTATATTGAACAACGATTCGGACAAGGATGTAAATAATGAAAAGGATGACATGTTCGAAACGA
TAACGGCTAAGGAGCAAGTT

Downstream 100 bases:

>100_bases
TAAGGACATAAGCAACTGAAATTGATGGCTGGCATGACGAGGGATGCAGATGCTGATTTCAATACCCCCGGTGATTATTG
AAGGAGAGACGAAAACGACT

Product: DNA-binding transcriptional activator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 175; Mature: 175

Protein sequence:

>175_residues
MIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIKKVGNKPFKVIIDTYHNHILDEEAIKFLEKLDAERIIVLAPYHISKL
KAKAPIYFVSRKESIKNLLEITYGKHLPHKNSQLCFSHNQFKIMQLILKNKNESNITSTLNISQQTLKIQKFNIMYKLKL
RRMSDIVTLGITSYF

Sequences:

>Translated_175_residues
MIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIKKVGNKPFKVIIDTYHNHILDEEAIKFLEKLDAERIIVLAPYHISKL
KAKAPIYFVSRKESIKNLLEITYGKHLPHKNSQLCFSHNQFKIMQLILKNKNESNITSTLNISQQTLKIQKFNIMYKLKL
RRMSDIVTLGITSYF
>Mature_175_residues
MIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIKKVGNKPFKVIIDTYHNHILDEEAIKFLEKLDAERIIVLAPYHISKL
KAKAPIYFVSRKESIKNLLEITYGKHLPHKNSQLCFSHNQFKIMQLILKNKNESNITSTLNISQQTLKIQKFNIMYKLKL
RRMSDIVTLGITSYF

Specific function: Regulates the expression of several genes involved in acid resistance. Required for the expression of gadA and gadBC, among others, regardless of media or growth conditions. Binds directly to the 20 bp GAD box found in the control regions of both loci. Co

COG id: COG2771

COG function: function code K; DNA-binding HTH domain-containing proteins

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH luxR-type DNA-binding domain

Homologues:

Organism=Escherichia coli, GI1789928, Length=175, Percent_Identity=100, Blast_Score=353, Evalue=3e-99,
Organism=Escherichia coli, GI1789923, Length=167, Percent_Identity=23.9520958083832, Blast_Score=67, Evalue=7e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GADE_ECO57 (P63206)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   A86024
- PIR:   H91177
- RefSeq:   NP_290092.1
- RefSeq:   NP_312419.1
- ProteinModelPortal:   P63206
- SMR:   P63206
- EnsemblBacteria:   EBESCT00000025686
- EnsemblBacteria:   EBESCT00000058961
- GeneID:   915751
- GeneID:   961156
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z4925
- KEGG:   ecs:ECs4392
- GeneTree:   EBGT00050000009146
- HOGENOM:   HBG471232
- OMA:   SELCFSH
- ProtClustDB:   CLSK880693
- BioCyc:   ECOL83334:ECS4392-MONOMER
- GO:   GO:0005622
- InterPro:   IPR016032
- InterPro:   IPR000792
- InterPro:   IPR011991
- InterPro:   IPR013022
- Gene3D:   G3DSA:1.10.10.10
- Gene3D:   G3DSA:3.20.20.150

Pfam domain/function: PF00196 GerE; SSF46894 Bipartite_resp_reg_C-effector

EC number: NA

Molecular weight: Translated: 20600; Mature: 20600

Theoretical pI: Translated: 10.36; Mature: 10.36

Prosite motif: PS00622 HTH_LUXR_1; PS50043 HTH_LUXR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIKKVGNKPFKVIIDTYHNHILDEEAIKF
CEEEEECCCHHHHHHHEECCCCCEEEECCHHHHHHHCCCCEEEEEEHHHHHCCCHHHHHH
LEKLDAERIIVLAPYHISKLKAKAPIYFVSRKESIKNLLEITYGKHLPHKNSQLCFSHNQ
HHHCCCCEEEEECCCHHHHHCCCCCEEEEECHHHHHHHHHHHHCCCCCCCCCCEEEECCH
FKIMQLILKNKNESNITSTLNISQQTLKIQKFNIMYKLKLRRMSDIVTLGITSYF
HHHHHHHHHCCCCCCCEEEEECCHHHEEEEEEEEEEEEEHHHHHHHEEEEEECCC
>Mature Secondary Structure
MIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIKKVGNKPFKVIIDTYHNHILDEEAIKF
CEEEEECCCHHHHHHHEECCCCCEEEECCHHHHHHHCCCCEEEEEEHHHHHCCCHHHHHH
LEKLDAERIIVLAPYHISKLKAKAPIYFVSRKESIKNLLEITYGKHLPHKNSQLCFSHNQ
HHHCCCCEEEEECCCHHHHHCCCCCEEEEECHHHHHHHHHHHHCCCCCCCCCCEEEECCH
FKIMQLILKNKNESNITSTLNISQQTLKIQKFNIMYKLKLRRMSDIVTLGITSYF
HHHHHHHHHCCCCCCCEEEEECCHHHEEEEEEEEEEEEEHHHHHHHEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796