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The map label for this gene is chuU

Identifier: 218702271

GI number: 218702271

Start: 4164774

End: 4165766

Strand: Direct

Name: chuU

Synonym: ECIAI39_4008

Alternate gene names: 218702271

Gene position: 4164774-4165766 (Clockwise)

Preceding gene: 218702270

Following gene: 218702272

Centisome position: 81.15

GC content: 52.77

Gene sequence:

>993_bases
GTGCTCAAGGATTCACTCTCTTCCGCCAGGGTCTTTATGGGGCTATCACTATTATTGCTCGCTCTGGTGCTGTTTGGTGC
CAGTCAGGGAGCGTTAAAGATCAGTTTTGATGCCCTTTTTGATGAGGAATACCGCGATATCTGGCTCAATATTCGTCTAC
CACGGGTTTTGCTGGCGGTGCTGGTAGGTGCAGCGTTGGCAACCGCCGGGGTAATTATGCAAGGGCTTTTTCGCAACCCA
ATGGCTGACCCTGGATTACTTGGCGTCAGTAGCGGTTCTGCATTAATGGTTGGCGTTGCTATTGTGCTGCCCTTCTCCTT
CCCAGTGGTGCTGGTGCTCTATGAGCAAATGGTGTTTGCCATTGCCGGAAGTTTAGTGGTCTGCACCATCATTTTTCTCA
TCACGCAGCGCCATCGCGATGGCAGCATGATGCAATTATTACTCGCCGGTATCGCCATCAATGCCCTGTGCGGCGCAGCG
ATCGGCATCCTGAGCTATATCGGCGATGAGCAGCAGCTACGACAACTCACATTGTGGATGATGGGCAATCTTGGACAGGC
GCAATGGCCGACGTTATTGGTTGCCAGTTCATTCATCCTACCGGCCATTATCGCAACAACTTGTCTCGCCGGAACGCTGA
ATTTACTGCAGCTCGGTGATGAAGAAGCCCACTACCTCGGCGTGAACGTTAAGCGTAAACGCCAGCAATTACTGTTAGTG
AGCTCACTGCTCGTTGGTGCCGCCGTATCGGTAAGCGGCATTATCGGCTTTATTGGCCTGGTGATCCCGCATCTGATTCG
TATGACTACCGGGGCAAATCATCGCTGGCTAATCCCTTGCTCCGCCCTCGCCGGAGCCTGTTTATTGCTGATGGCAGACA
CGCTTGCCCGCACACTGGTACAGCCAGCAGAAATGCCCGTGGGATTATTAACCAGCCTGCTTGGTGGCCCTTATTTTATG
TGGTTGATTCTGCGCAACCGGAGGATCACATGA

Upstream 100 bases:

>100_bases
ACTTAACCAGCAGGTCTACAGTCTGATTGAACCGGACCTGAAACCGGCGTGATCCCCCGGCGCGGTATGTCCGCGCCGTA
ATGTCGTAACGGAGTTTTGC

Downstream 100 bases:

>100_bases
TCAGCGCCCAGAATCTGGTTTATAGCCTTCAGGGGCGGCGTCTGACTGATAATGTCTCGCTGACTTTTCCCGGTGGGGAA
ATTGTTGCCATCCTCGGCCC

Product: putative hemin transport system permease

Products: ADP; phosphate; iron dicitrate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 330; Mature: 330

Protein sequence:

>330_residues
MLKDSLSSARVFMGLSLLLLALVLFGASQGALKISFDALFDEEYRDIWLNIRLPRVLLAVLVGAALATAGVIMQGLFRNP
MADPGLLGVSSGSALMVGVAIVLPFSFPVVLVLYEQMVFAIAGSLVVCTIIFLITQRHRDGSMMQLLLAGIAINALCGAA
IGILSYIGDEQQLRQLTLWMMGNLGQAQWPTLLVASSFILPAIIATTCLAGTLNLLQLGDEEAHYLGVNVKRKRQQLLLV
SSLLVGAAVSVSGIIGFIGLVIPHLIRMTTGANHRWLIPCSALAGACLLLMADTLARTLVQPAEMPVGLLTSLLGGPYFM
WLILRNRRIT

Sequences:

>Translated_330_residues
MLKDSLSSARVFMGLSLLLLALVLFGASQGALKISFDALFDEEYRDIWLNIRLPRVLLAVLVGAALATAGVIMQGLFRNP
MADPGLLGVSSGSALMVGVAIVLPFSFPVVLVLYEQMVFAIAGSLVVCTIIFLITQRHRDGSMMQLLLAGIAINALCGAA
IGILSYIGDEQQLRQLTLWMMGNLGQAQWPTLLVASSFILPAIIATTCLAGTLNLLQLGDEEAHYLGVNVKRKRQQLLLV
SSLLVGAAVSVSGIIGFIGLVIPHLIRMTTGANHRWLIPCSALAGACLLLMADTLARTLVQPAEMPVGLLTSLLGGPYFM
WLILRNRRIT
>Mature_330_residues
MLKDSLSSARVFMGLSLLLLALVLFGASQGALKISFDALFDEEYRDIWLNIRLPRVLLAVLVGAALATAGVIMQGLFRNP
MADPGLLGVSSGSALMVGVAIVLPFSFPVVLVLYEQMVFAIAGSLVVCTIIFLITQRHRDGSMMQLLLAGIAINALCGAA
IGILSYIGDEQQLRQLTLWMMGNLGQAQWPTLLVASSFILPAIIATTCLAGTLNLLQLGDEEAHYLGVNVKRKRQQLLLV
SSLLVGAAVSVSGIIGFIGLVIPHLIRMTTGANHRWLIPCSALAGACLLLMADTLARTLVQPAEMPVGLLTSLLGGPYFM
WLILRNRRIT

Specific function: Part of the binding-protein-dependent transport system for hemin; probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG0609

COG function: function code P; ABC-type Fe3+-siderophore transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the binding-protein-dependent transport system permease family. FecCD subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790740, Length=319, Percent_Identity=36.3636363636364, Blast_Score=168, Evalue=4e-43,
Organism=Escherichia coli, GI1788004, Length=279, Percent_Identity=41.2186379928315, Blast_Score=164, Evalue=9e-42,
Organism=Escherichia coli, GI1790741, Length=279, Percent_Identity=39.0681003584229, Blast_Score=153, Evalue=2e-38,
Organism=Escherichia coli, GI1786805, Length=300, Percent_Identity=37.3333333333333, Blast_Score=126, Evalue=2e-30,
Organism=Escherichia coli, GI1786804, Length=312, Percent_Identity=33.974358974359, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI1786347, Length=326, Percent_Identity=33.7423312883436, Blast_Score=99, Evalue=5e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000522 [H]

Pfam domain/function: PF01032 FecCD [H]

EC number: NA

Molecular weight: Translated: 35348; Mature: 35348

Theoretical pI: Translated: 8.74; Mature: 8.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKDSLSSARVFMGLSLLLLALVLFGASQGALKISFDALFDEEYRDIWLNIRLPRVLLAV
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHEEEEEEHHHHHHHH
LVGAALATAGVIMQGLFRNPMADPGLLGVSSGSALMVGVAIVLPFSFPVVLVLYEQMVFA
HHHHHHHHHHHHHHHHHCCCCCCCCCEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
IAGSLVVCTIIFLITQRHRDGSMMQLLLAGIAINALCGAAIGILSYIGDEQQLRQLTLWM
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
MGNLGQAQWPTLLVASSFILPAIIATTCLAGTLNLLQLGDEEAHYLGVNVKRKRQQLLLV
HCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEECCCHHHHHHHHHHH
SSLLVGAAVSVSGIIGFIGLVIPHLIRMTTGANHRWLIPCSALAGACLLLMADTLARTLV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHH
QPAEMPVGLLTSLLGGPYFMWLILRNRRIT
CHHCCCHHHHHHHHCCHHHHHHHHHCCCCC
>Mature Secondary Structure
MLKDSLSSARVFMGLSLLLLALVLFGASQGALKISFDALFDEEYRDIWLNIRLPRVLLAV
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHEEEEEEHHHHHHHH
LVGAALATAGVIMQGLFRNPMADPGLLGVSSGSALMVGVAIVLPFSFPVVLVLYEQMVFA
HHHHHHHHHHHHHHHHHCCCCCCCCCEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
IAGSLVVCTIIFLITQRHRDGSMMQLLLAGIAINALCGAAIGILSYIGDEQQLRQLTLWM
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
MGNLGQAQWPTLLVASSFILPAIIATTCLAGTLNLLQLGDEEAHYLGVNVKRKRQQLLLV
HCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEECCCHHHHHHHHHHH
SSLLVGAAVSVSGIIGFIGLVIPHLIRMTTGANHRWLIPCSALAGACLLLMADTLARTLV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHH
QPAEMPVGLLTSLLGGPYFMWLILRNRRIT
CHHCCCHHHHHHHHCCHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; iron dicitrate [Periplasm]; H2O [C]

Specific reaction: ATP + iron dicitrate [Periplasm] + H2O = ADP + phosphate + iron dicitrate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9026634; 11586360; 12142430 [H]