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The map label for this gene is glpK [H]

Identifier: 218702231

GI number: 218702231

Start: 4123126

End: 4124634

Strand: Direct

Name: glpK [H]

Synonym: ECIAI39_3967

Alternate gene names: 218702231

Gene position: 4123126-4124634 (Clockwise)

Preceding gene: 218702230

Following gene: 218702232

Centisome position: 80.34

GC content: 54.47

Gene sequence:

>1509_bases
ATGCCTGATAACAGCGCAGCTATCGTTATCGATATTGGCACCACCAATTGCAAAGTCACCTGCTTTTCCTGCCTGGACGC
AACGACGTTGGGCGCGCATAAATTCGTGACGGCAAAACAGATCTCCCCACAGGGCGATGTCGATTTCGATATCGACGCCC
TCTGGCAGGAGGTCCGCCAGGCGATAGCGCAACTGAACGCCGCTTCGCCGCTGCCAGTCAGGCGGATAAGCATTGCCAGT
TTTGGCGAGTCAGGCGTGTTCCTTGACGAGCTCGGCGAGATCCTGACGCCAATGCTGGCATGGTATGACCGCCGCGGTGA
AGAGTACCTGGCAACGCTTAGTGAGGCAGACAGTGCGGCGCTTTATGACATCTGCGGGCTACCGCTGCACAGCAATTACT
CTGCCTTCAAAATGCGTTGGTTGCTGGAGCATTATCCACTGCGTAATCGCCGCGGCCTGCGTTGGCTACATGCGCCGGAA
GTACTGCTCTGGCGGCTGACTGGCGAACAGCGTACGGATATCACCTTAGCCAGCCGTACGCTGTGTCTGGACGTGCGCAA
AGGCGAATGGTCAGCGAAAGCGGCGGCGTTGTTACACGTTCCCTGTTCAGCATTTGCGCCATTGGTGCAGCCAGGCGAGC
ACGCCGGATGGGTCAGCGAGTCACTTTGCAAGACACTTGGGTTCTCGCAACCGGTCAGCGTGACGCTGGCCGGACATGAC
CATATGGTGGGTGCGCGGGCGTTGCAGATGATGCCGGGCGATATCCTTAACTCGACGGGGACCACAGAAGGCATTCTGCA
ACTGGATACACAACCGACGCTGGATGAACAGGCCAAACGTGACAAGCTGGCAAACGGCTGTTATTCGCTTGCAAACCAGT
TCACCCTGTTTGCGTCGCTGCCCGTGGGCGGTTTCGCTCTGGAGTGGCTGCGTAACACGTTCCGGCTAACCGATGAGGAG
ATCGCCGCATCACTTACTCGCGGACATGCGGATTACCTGGCAGGGAACTGGTCGCTCGATGACATTCCCGTTTTTATTCC
GCATCTTCGCGGTTCGGGTTCGCCCTATAAAAATCGCCATACCCGAGGATTATTTTATGGGCTTGGCGATACGTTAAATA
TCGATATGTTAATTGCCAGCGTCTCACTGGGATTAACCATGGAATTTGCCAACTGCTTCGCCTGTTTTAATGTGCCCGGC
ACCAGCGCGTTAAAAGTGATAGGTCCGGCAACCCATAATCCCCTTTGGCTGCAATTAAAGGCGGATATTTTACAGCGTCC
GGTTGAAGCAATTGCATTTAACGAGGCGGTTTCTGTCGGAGCATTATTAACCGCCGCACCGGATATTCCACCGCCGCAAG
TCACCATAGCCCAACGTTTGTTACCGAATCGGGCGAGATACCATCAATTACAGCGTTATCAGCACAAATGGAAAAGCTGG
TATCAGTTGAAATTACAGCAAGAAGGCGTGATGCCATTACATCATCGGGAGGAGCACTATGTTGAGTAA

Upstream 100 bases:

>100_bases
CTGCTGGAAATCTTCAACGGCAATCTCATCGCCATTGGTCTGGTGCCGGTTATCGCCCTGGTACTGTATGGCATTTTCCG
AATGACGCGGAGCACGGTTT

Downstream 100 bases:

>100_bases
AACCGTGGAGGTCAGAAACAGTACTGGCCTGCATGCACGTCCGGCGGCCTGTCTGGCGAAAGCCGCCAAAAAATATAGCT
GTAAAGTGACGCTGCATTAT

Product: putative carbohydrate kinase

Products: NA

Alternate protein names: ATP:glycerol 3-phosphotransferase; Glycerokinase; GK [H]

Number of amino acids: Translated: 502; Mature: 501

Protein sequence:

>502_residues
MPDNSAAIVIDIGTTNCKVTCFSCLDATTLGAHKFVTAKQISPQGDVDFDIDALWQEVRQAIAQLNAASPLPVRRISIAS
FGESGVFLDELGEILTPMLAWYDRRGEEYLATLSEADSAALYDICGLPLHSNYSAFKMRWLLEHYPLRNRRGLRWLHAPE
VLLWRLTGEQRTDITLASRTLCLDVRKGEWSAKAAALLHVPCSAFAPLVQPGEHAGWVSESLCKTLGFSQPVSVTLAGHD
HMVGARALQMMPGDILNSTGTTEGILQLDTQPTLDEQAKRDKLANGCYSLANQFTLFASLPVGGFALEWLRNTFRLTDEE
IAASLTRGHADYLAGNWSLDDIPVFIPHLRGSGSPYKNRHTRGLFYGLGDTLNIDMLIASVSLGLTMEFANCFACFNVPG
TSALKVIGPATHNPLWLQLKADILQRPVEAIAFNEAVSVGALLTAAPDIPPPQVTIAQRLLPNRARYHQLQRYQHKWKSW
YQLKLQQEGVMPLHHREEHYVE

Sequences:

>Translated_502_residues
MPDNSAAIVIDIGTTNCKVTCFSCLDATTLGAHKFVTAKQISPQGDVDFDIDALWQEVRQAIAQLNAASPLPVRRISIAS
FGESGVFLDELGEILTPMLAWYDRRGEEYLATLSEADSAALYDICGLPLHSNYSAFKMRWLLEHYPLRNRRGLRWLHAPE
VLLWRLTGEQRTDITLASRTLCLDVRKGEWSAKAAALLHVPCSAFAPLVQPGEHAGWVSESLCKTLGFSQPVSVTLAGHD
HMVGARALQMMPGDILNSTGTTEGILQLDTQPTLDEQAKRDKLANGCYSLANQFTLFASLPVGGFALEWLRNTFRLTDEE
IAASLTRGHADYLAGNWSLDDIPVFIPHLRGSGSPYKNRHTRGLFYGLGDTLNIDMLIASVSLGLTMEFANCFACFNVPG
TSALKVIGPATHNPLWLQLKADILQRPVEAIAFNEAVSVGALLTAAPDIPPPQVTIAQRLLPNRARYHQLQRYQHKWKSW
YQLKLQQEGVMPLHHREEHYVE
>Mature_501_residues
PDNSAAIVIDIGTTNCKVTCFSCLDATTLGAHKFVTAKQISPQGDVDFDIDALWQEVRQAIAQLNAASPLPVRRISIASF
GESGVFLDELGEILTPMLAWYDRRGEEYLATLSEADSAALYDICGLPLHSNYSAFKMRWLLEHYPLRNRRGLRWLHAPEV
LLWRLTGEQRTDITLASRTLCLDVRKGEWSAKAAALLHVPCSAFAPLVQPGEHAGWVSESLCKTLGFSQPVSVTLAGHDH
MVGARALQMMPGDILNSTGTTEGILQLDTQPTLDEQAKRDKLANGCYSLANQFTLFASLPVGGFALEWLRNTFRLTDEEI
AASLTRGHADYLAGNWSLDDIPVFIPHLRGSGSPYKNRHTRGLFYGLGDTLNIDMLIASVSLGLTMEFANCFACFNVPGT
SALKVIGPATHNPLWLQLKADILQRPVEAIAFNEAVSVGALLTAAPDIPPPQVTIAQRLLPNRARYHQLQRYQHKWKSWY
QLKLQQEGVMPLHHREEHYVE

Specific function: Key enzyme in the regulation of glycerol uptake and metabolism [H]

COG id: COG1070

COG function: function code G; Sugar (pentulose and hexulose) kinases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FGGY kinase family [H]

Homologues:

Organism=Homo sapiens, GI41393575, Length=447, Percent_Identity=24.6085011185682, Blast_Score=90, Evalue=5e-18,
Organism=Homo sapiens, GI42794763, Length=453, Percent_Identity=24.5033112582781, Blast_Score=89, Evalue=1e-17,
Organism=Homo sapiens, GI4504007, Length=451, Percent_Identity=24.390243902439, Blast_Score=88, Evalue=2e-17,
Organism=Homo sapiens, GI189409120, Length=451, Percent_Identity=24.390243902439, Blast_Score=87, Evalue=2e-17,
Organism=Escherichia coli, GI1789168, Length=460, Percent_Identity=26.304347826087, Blast_Score=104, Evalue=2e-23,
Organism=Escherichia coli, GI1789987, Length=490, Percent_Identity=22.8571428571429, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1790361, Length=462, Percent_Identity=22.2943722943723, Blast_Score=80, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17535599, Length=447, Percent_Identity=23.7136465324385, Blast_Score=98, Evalue=9e-21,
Organism=Drosophila melanogaster, GI17864214, Length=534, Percent_Identity=23.4082397003745, Blast_Score=95, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24654573, Length=460, Percent_Identity=24.1304347826087, Blast_Score=94, Evalue=3e-19,
Organism=Drosophila melanogaster, GI19922058, Length=407, Percent_Identity=22.1130221130221, Blast_Score=69, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000577
- InterPro:   IPR018485
- InterPro:   IPR018483
- InterPro:   IPR018484
- InterPro:   IPR005999 [H]

Pfam domain/function: PF02782 FGGY_C; PF00370 FGGY_N [H]

EC number: =2.7.1.30 [H]

Molecular weight: Translated: 55619; Mature: 55488

Theoretical pI: Translated: 6.55; Mature: 6.55

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPDNSAAIVIDIGTTNCKVTCFSCLDATTLGAHKFVTAKQISPQGDVDFDIDALWQEVRQ
CCCCCCEEEEEECCCCCEEEEEEHHCCHHCCCHHEEEECCCCCCCCCCCCHHHHHHHHHH
AIAQLNAASPLPVRRISIASFGESGVFLDELGEILTPMLAWYDRRGEEYLATLSEADSAA
HHHHCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCHH
LYDICGLPLHSNYSAFKMRWLLEHYPLRNRRGLRWLHAPEVLLWRLTGEQRTDITLASRT
HHHHCCCCCCCCCHHHHHHHHHHHCCCCCCCCCEEEECCCEEEEEECCCCCCCEEEECEE
LCLDVRKGEWSAKAAALLHVPCSAFAPLVQPGEHAGWVSESLCKTLGFSQPVSVTLAGHD
EEEEECCCCCCCCEEEEEECCHHHHCCCCCCCCCCCHHHHHHHHHCCCCCCEEEEECCCC
HMVGARALQMMPGDILNSTGTTEGILQLDTQPTLDEQAKRDKLANGCYSLANQFTLFASL
CHHHHHHHHHCCCHHHCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHCEEEEEEC
PVGGFALEWLRNTFRLTDEEIAASLTRGHADYLAGNWSLDDIPVFIPHLRGSGSPYKNRH
CCCHHHHHHHHHHHCCCHHHHHHHHHCCCHHHEECCCCCCCCCEEEECCCCCCCCCCCCC
TRGLFYGLGDTLNIDMLIASVSLGLTMEFANCFACFNVPGTSALKVIGPATHNPLWLQLK
CCEEEEECCCCCCHHHEEEHHHHCEEEEHHCEEEEECCCCCCEEEEECCCCCCCEEEEEE
ADILQRPVEAIAFNEAVSVGALLTAAPDIPPPQVTIAQRLLPNRARYHQLQRYQHKWKSW
HHHHHCHHHHHHHHHHHHHCCCEECCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHH
YQLKLQQEGVMPLHHREEHYVE
EEEEEECCCCCCCCCCHHHCCC
>Mature Secondary Structure 
PDNSAAIVIDIGTTNCKVTCFSCLDATTLGAHKFVTAKQISPQGDVDFDIDALWQEVRQ
CCCCCEEEEEECCCCCEEEEEEHHCCHHCCCHHEEEECCCCCCCCCCCCHHHHHHHHHH
AIAQLNAASPLPVRRISIASFGESGVFLDELGEILTPMLAWYDRRGEEYLATLSEADSAA
HHHHCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCHH
LYDICGLPLHSNYSAFKMRWLLEHYPLRNRRGLRWLHAPEVLLWRLTGEQRTDITLASRT
HHHHCCCCCCCCCHHHHHHHHHHHCCCCCCCCCEEEECCCEEEEEECCCCCCCEEEECEE
LCLDVRKGEWSAKAAALLHVPCSAFAPLVQPGEHAGWVSESLCKTLGFSQPVSVTLAGHD
EEEEECCCCCCCCEEEEEECCHHHHCCCCCCCCCCCHHHHHHHHHCCCCCCEEEEECCCC
HMVGARALQMMPGDILNSTGTTEGILQLDTQPTLDEQAKRDKLANGCYSLANQFTLFASL
CHHHHHHHHHCCCHHHCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHCEEEEEEC
PVGGFALEWLRNTFRLTDEEIAASLTRGHADYLAGNWSLDDIPVFIPHLRGSGSPYKNRH
CCCHHHHHHHHHHHCCCHHHHHHHHHCCCHHHEECCCCCCCCCEEEECCCCCCCCCCCCC
TRGLFYGLGDTLNIDMLIASVSLGLTMEFANCFACFNVPGTSALKVIGPATHNPLWLQLK
CCEEEEECCCCCCHHHEEEHHHHCEEEEHHCEEEEECCCCCCEEEEECCCCCCCEEEEEE
ADILQRPVEAIAFNEAVSVGALLTAAPDIPPPQVTIAQRLLPNRARYHQLQRYQHKWKSW
HHHHHCHHHHHHHHHHHHHCCCEECCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHH
YQLKLQQEGVMPLHHREEHYVE
EEEEEECCCCCCCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11337471 [H]