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The map label for this gene is gatB [H]

Identifier: 218702229

GI number: 218702229

Start: 4121420

End: 4121701

Strand: Direct

Name: gatB [H]

Synonym: ECIAI39_3965

Alternate gene names: 218702229

Gene position: 4121420-4121701 (Clockwise)

Preceding gene: 218702228

Following gene: 218702230

Centisome position: 80.31

GC content: 43.97

Gene sequence:

>282_bases
ATGAGTCAAACCATTTTATTTGTCTGCGCCACGGGTATTGCCACATCCACGGCGGTAACAGAAAAAGTCATGGAATATTG
TAAAGAGCACGGTCTTAACGTCAATTATTCCCAAACCAACGTTGCCTCTTTACCTGGAAATACTGATGGCGTAGCACTGG
TAGTCTCAACGACTAAAGTGCCTTATGAACTCGACGTGCCGGTGGTTAACGGCTTACCCATTATTACCGGTATTGGCGAA
GAGAAAGTGCTGGCGCAAATTGTTTCGATTCTTAAAAAGTAA

Upstream 100 bases:

>100_bases
AGATGATTCAGGATACGCCCTTTATGCAAGCCTTGCTTACGATGGAAACAACAGAAATACACACAGTAATCTTAAACAAA
ATGAAAGAACGAGGTGAAAT

Downstream 100 bases:

>100_bases
TTAATAATTAATTCCAACCCACTCCATCAGGCGCTAACGCGAGGTGCGCTCTCGCCTGAAAAAGAGGTTTATTATGAACG
ATATCGCGCATACCCTCTAT

Product: putative phosphotransferase system enzyme IIB

Products: NA

Alternate protein names: EIIB-Gat; PTS system galactitol-specific EIIB component [H]

Number of amino acids: Translated: 93; Mature: 92

Protein sequence:

>93_residues
MSQTILFVCATGIATSTAVTEKVMEYCKEHGLNVNYSQTNVASLPGNTDGVALVVSTTKVPYELDVPVVNGLPIITGIGE
EKVLAQIVSILKK

Sequences:

>Translated_93_residues
MSQTILFVCATGIATSTAVTEKVMEYCKEHGLNVNYSQTNVASLPGNTDGVALVVSTTKVPYELDVPVVNGLPIITGIGE
EKVLAQIVSILKK
>Mature_92_residues
SQTILFVCATGIATSTAVTEKVMEYCKEHGLNVNYSQTNVASLPGNTDGVALVVSTTKVPYELDVPVVNGLPIITGIGEE
KVLAQIVSILKK

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitant with their translocation across the cell membrane. This system i

COG id: COG3414

COG function: function code G; Phosphotransferase system, galactitol-specific IIB component

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIB type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI1788409, Length=93, Percent_Identity=36.5591397849462, Blast_Score=67, Evalue=2e-13,
Organism=Escherichia coli, GI87082416, Length=89, Percent_Identity=30.3370786516854, Blast_Score=63, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013011
- InterPro:   IPR003501 [H]

Pfam domain/function: PF02302 PTS_IIB [H]

EC number: =2.7.1.69 [H]

Molecular weight: Translated: 9823; Mature: 9691

Theoretical pI: Translated: 4.91; Mature: 4.91

Prosite motif: PS51099 PTS_EIIB_TYPE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQTILFVCATGIATSTAVTEKVMEYCKEHGLNVNYSQTNVASLPGNTDGVALVVSTTKV
CCCEEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCEEEEEECCCC
PYELDVPVVNGLPIITGIGEEKVLAQIVSILKK
CEEECCCEECCEEEEEECCHHHHHHHHHHHHCC
>Mature Secondary Structure 
SQTILFVCATGIATSTAVTEKVMEYCKEHGLNVNYSQTNVASLPGNTDGVALVVSTTKV
CCEEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCEEEEEECCCC
PYELDVPVVNGLPIITGIGEEKVLAQIVSILKK
CEEECCCEECCEEEEEECCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]