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The map label for this gene is nirC

Identifier: 218702116

GI number: 218702116

Start: 3996272

End: 3997078

Strand: Direct

Name: nirC

Synonym: ECIAI39_3851

Alternate gene names: 218702116

Gene position: 3996272-3997078 (Clockwise)

Preceding gene: 218702115

Following gene: 218702117

Centisome position: 77.87

GC content: 54.52

Gene sequence:

>807_bases
ATGTTTACAGACACTATTAATAAGTGTGCGGCTAACGCTGCGCGCATTGCACGCCTGTCGGCAAATAACCCGCTCGGCTT
CTGGGTCAGCTCCGCAATGGCGGGGGCGTATGTGGGTCTTGGGATCATCCTGATTTTCACGCTCGGTAATTTGCTCGACC
CGTCCGTACGTCCTCTGGTGATGGGCGCGACCTTTGGTATCGCCTTAACGCTGGTCATTATTGCTGGTTCTGAACTGTTC
ACCGGACACACCATGTTCCTCACCTTTGGAGTGAAAGCGGGCACTATCAGCCACGGGCAAATGTGGGCAATCCTGCCGCA
AACCTGGCTGGGTAACCTGGTTGGTTCCGTCTTTGTTGCCATGCTCTATAGCTGGGGCGGCGGTAGCCTGCTGCCGGTAG
ATACCAGCATCGTTCACTCCGTCGCGCTGGCTAAAACCACTGCACCGGCAATGGTACTGTTCTTCAAAGGTGCATTGTGT
AACTGGCTGGTTTGCCTGGCTATCTGGATGGCGCTGCGCACTGAAGGGGCGGCGAAATTTATCGCTATCTGGTGGTGTCT
GCTGGCATTTATCGCGTCCGGCTACGAGCACTCCATCGCGAACATGACGCTGTTCGCCCTCTCCTGGTTTGGCAACCACA
GCGAAGCCTACACGCTGGCGGGTATTGGTCATAACCTGCTGTGGGTGACGCTGGGTAATACTTTATCAGGTGCCGTATTC
ATGGGATTGGGTTATTGGTATGCTACGCCGAAAGCGAATCGTCCGGTTGCGGACAAATTTAATCAAACTGAAACAGCTGC
CGGTTAA

Upstream 100 bases:

>100_bases
CCCCTTTTTGCATGGTCCTGTAATAACCGTCGGTATATTGCAGGACATTTTTTAAACTTTTTGTTTTATTTTTTGTTTTT
ATTTTTTAAAGGATAATCAA

Downstream 100 bases:

>100_bases
TTACTAAGGGGTTTTTACGTGGATCATTTGCCTATATTTTGCCAATTACGCGATCGTGACTGTCTGATTGTCGGCGGTGG
TGATGTCGCGGAACGCAAAG

Product: nitrite transporter NirC

Products: Proton [Cytoplasm]; nitrite [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 268; Mature: 268

Protein sequence:

>268_residues
MFTDTINKCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIILIFTLGNLLDPSVRPLVMGATFGIALTLVIIAGSELF
TGHTMFLTFGVKAGTISHGQMWAILPQTWLGNLVGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALC
NWLVCLAIWMALRTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYTLAGIGHNLLWVTLGNTLSGAVF
MGLGYWYATPKANRPVADKFNQTETAAG

Sequences:

>Translated_268_residues
MFTDTINKCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIILIFTLGNLLDPSVRPLVMGATFGIALTLVIIAGSELF
TGHTMFLTFGVKAGTISHGQMWAILPQTWLGNLVGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALC
NWLVCLAIWMALRTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYTLAGIGHNLLWVTLGNTLSGAVF
MGLGYWYATPKANRPVADKFNQTETAAG
>Mature_268_residues
MFTDTINKCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIILIFTLGNLLDPSVRPLVMGATFGIALTLVIIAGSELF
TGHTMFLTFGVKAGTISHGQMWAILPQTWLGNLVGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALC
NWLVCLAIWMALRTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYTLAGIGHNLLWVTLGNTLSGAVF
MGLGYWYATPKANRPVADKFNQTETAAG

Specific function: May act as a nitrite transporter [H]

COG id: COG2116

COG function: function code P; Formate/nitrite family of transporters

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FNT transporter (TC 2.A.44) family [H]

Homologues:

Organism=Escherichia coli, GI87082249, Length=268, Percent_Identity=99.6268656716418, Blast_Score=533, Evalue=1e-153,
Organism=Escherichia coli, GI1787132, Length=268, Percent_Identity=28.7313432835821, Blast_Score=75, Evalue=6e-15,
Organism=Saccharomyces cerevisiae, GI6321779, Length=211, Percent_Identity=28.9099526066351, Blast_Score=70, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000292 [H]

Pfam domain/function: PF01226 Form_Nir_trans [H]

EC number: NA

Molecular weight: Translated: 28577; Mature: 28577

Theoretical pI: Translated: 8.63; Mature: 8.63

Prosite motif: PS01005 FORMATE_NITRITE_TP_1 ; PS01006 FORMATE_NITRITE_TP_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFTDTINKCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIILIFTLGNLLDPSVRPLV
CCCCHHHHHHHHHHHHHEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEE
MGATFGIALTLVIIAGSELFTGHTMFLTFGVKAGTISHGQMWAILPQTWLGNLVGSVFVA
EHHHHHHHHHHHHHCCCCHHCCEEEEEEECCCCCCCCCCCEEEECHHHHHHHHHHHHHHH
MLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALCNWLVCLAIWMALRTEGAAKF
HHHHCCCCCEEECCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
IAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYTLAGIGHNLLWVTLGNTLSGAVF
HHHHHHHHHHHHCCCHHHHHHHHEEEEECCCCCCCEEEEECCCCCEEEEEECCCCCCHHH
MGLGYWYATPKANRPVADKFNQTETAAG
HHHHHEEECCCCCCCHHHHCCCCCCCCC
>Mature Secondary Structure
MFTDTINKCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIILIFTLGNLLDPSVRPLV
CCCCHHHHHHHHHHHHHEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEE
MGATFGIALTLVIIAGSELFTGHTMFLTFGVKAGTISHGQMWAILPQTWLGNLVGSVFVA
EHHHHHHHHHHHHHCCCCHHCCEEEEEEECCCCCCCCCCCEEEECHHHHHHHHHHHHHHH
MLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALCNWLVCLAIWMALRTEGAAKF
HHHHCCCCCEEECCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
IAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYTLAGIGHNLLWVTLGNTLSGAVF
HHHHHHHHHHHHCCCHHHHHHHHEEEEECCCCCCCEEEEECCCCCEEEEEECCCCCCHHH
MGLGYWYATPKANRPVADKFNQTETAAG
HHHHHEEECCCCCCCHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; nitrite [Periplasm] [C]

Specific reaction: Proton [Periplasm] + nitrite [Periplasm] = Proton [Cytoplasm] + nitrite [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 1987123; 11677609 [H]