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The map label for this gene is gltD

Identifier: 218701982

GI number: 218701982

Start: 3868816

End: 3870234

Strand: Direct

Name: gltD

Synonym: ECIAI39_3708

Alternate gene names: 218701982

Gene position: 3868816-3870234 (Clockwise)

Preceding gene: 218701981

Following gene: 218701983

Centisome position: 75.39

GC content: 55.67

Gene sequence:

>1419_bases
ATGAGTCAGAATGTTTATCAATTTATCGACCTGCAGCGCGTTGATCCGCCAAAGAAACCGCTGAAGATCCGCAAAATTGA
GTTTGTTGAAATTTACGAGCCGTTTTCCGAAGGCCAGGCCAAAGCGCAGGCTGACCGCTGCCTGTCGTGCGGCAACCCAT
ACTGCGAGTGGAAATGCCCGGTACACAACTACATCCCGAACTGGCTGAAGCTTGCCAACGAGGGGCGTATTTTTGAAGCG
GCAGAACTGTCGCACCAGACCAATACCCTGCCGGAAGTTTGCGGACGTGTCTGCCCGCAAGACCGTCTGTGCGAAGGTTC
CTGCACCCTGAACGATGAGTTTGGCGCAGTGACCATCGGCAACATTGAGCGTTATATCAATGATAAAGCGTTCGAAATGG
GCTGGCGTCCGGATATGTCTGGCGTGAAACAGACCGGTAAAAAAGTAGCGATTATCGGTGCAGGCCCGGCGGGTCTGGCG
TGTGCAGATGTCCTGACGCGCAACGGCGTAAAAGCAGTCGTCTTCGACCGTCACCCGGAAATTGGCGGCCTGCTGACCTT
CGGTATTCCGGCCTTCAAGCTGGAAAAAGAGGTAATGACGCGCCGCCGTGAAATCTTCACGGGCATGGGTATTGAATTCA
AACTCAATACCGAAGTGGGCCGCGACGTACAGCTGGACGATCTGCTGAGTGATTACGATGCCGTGTTCCTTGGCGTCGGG
ACTTATCAGTCAATGCGCGGCGGGCTGGAAAACGAAGACGCCGATGGCGTGTACGCAGCGCTGCCGTTCCTTATCGCCAA
CACCAAACAGTTAATGGGCTTTGGCGAAACTCGCGAAGAACCGTTCGTCAGCATGGAAGGCAAACGCGTGGTGGTCCTTG
GCGGTGGCGACACTGCGATGGACTGCGTGCGCACGTCTGTACGCCAGGGGGCAAAGCACGTTACCTGTGCCTATCGTCGT
GATGAAGAGAACATGCCGGGTTCCCGCCGCGAAGTGAAAAACGCGCGGGAAGAAGGTGTTGAGTTCAAATTCAACGTCCA
GCCGCTGGGTATAGAAGTGAATGGCAACGGCAAAGTCAGCGGCGTAAAAATGGTGCGCACAGAAATGGGCGAACCGGATG
CCAAAGGCCGTCGCCGCGCAGAGATCGTGGCAGGGTCAGAACATATCGTTCCGGCAGATGCGGTGATCATGGCGTTTGGT
TTCCGTCCACACAGCATGGAATGGCTGGCAAAACACAGCGTCGAGCTGGATTCGCAGGGGCGCATTATCGCCCCGGAAGG
CAACGACAACGCTTTCCAGACCAGCAACCCGAAAATCTTTGCTGGCGGCGATATCGTCCGTGGTTCCGATCTGGTCGTTA
CCGCCATTGCCGAAGGTCGCAAAGCGGCAGACGGCATTATGAACTGGCTGGAAGTTTAA

Upstream 100 bases:

>100_bases
GCTGGTTAAACCGAAGTCCAGTGATGTAAAAGCACTGCTGGGTCACCGTAGTCGTAGCGCAGCAGAGTTGCGCGTGCAGG
CGCAGTAAGGGGTAGCAACA

Downstream 100 bases:

>100_bases
GCTCTGCCTCAGCGCCGCCTTCTCAGAAAAGAGTCGGCGCTGAAATTCTCCTTTCGTTCTGGAAAGCGCCTCGCATAATC
TGAAGAAAACCAATGTAATA

Product: glutamate synthase subunit beta

Products: NA

Alternate protein names: Glutamate synthase subunit beta; GLTS beta chain; NADPH-GOGAT [H]

Number of amino acids: Translated: 472; Mature: 471

Protein sequence:

>472_residues
MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQADRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEA
AELSHQTNTLPEVCGRVCPQDRLCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDMSGVKQTGKKVAIIGAGPAGLA
CADVLTRNGVKAVVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTEVGRDVQLDDLLSDYDAVFLGVG
TYQSMRGGLENEDADGVYAALPFLIANTKQLMGFGETREEPFVSMEGKRVVVLGGGDTAMDCVRTSVRQGAKHVTCAYRR
DEENMPGSRREVKNAREEGVEFKFNVQPLGIEVNGNGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHIVPADAVIMAFG
FRPHSMEWLAKHSVELDSQGRIIAPEGNDNAFQTSNPKIFAGGDIVRGSDLVVTAIAEGRKAADGIMNWLEV

Sequences:

>Translated_472_residues
MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQADRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEA
AELSHQTNTLPEVCGRVCPQDRLCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDMSGVKQTGKKVAIIGAGPAGLA
CADVLTRNGVKAVVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTEVGRDVQLDDLLSDYDAVFLGVG
TYQSMRGGLENEDADGVYAALPFLIANTKQLMGFGETREEPFVSMEGKRVVVLGGGDTAMDCVRTSVRQGAKHVTCAYRR
DEENMPGSRREVKNAREEGVEFKFNVQPLGIEVNGNGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHIVPADAVIMAFG
FRPHSMEWLAKHSVELDSQGRIIAPEGNDNAFQTSNPKIFAGGDIVRGSDLVVTAIAEGRKAADGIMNWLEV
>Mature_471_residues
SQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQADRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEAA
ELSHQTNTLPEVCGRVCPQDRLCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDMSGVKQTGKKVAIIGAGPAGLAC
ADVLTRNGVKAVVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTEVGRDVQLDDLLSDYDAVFLGVGT
YQSMRGGLENEDADGVYAALPFLIANTKQLMGFGETREEPFVSMEGKRVVVLGGGDTAMDCVRTSVRQGAKHVTCAYRRD
EENMPGSRREVKNAREEGVEFKFNVQPLGIEVNGNGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHIVPADAVIMAFGF
RPHSMEWLAKHSVELDSQGRIIAPEGNDNAFQTSNPKIFAGGDIVRGSDLVVTAIAEGRKAADGIMNWLEV

Specific function: NITROGEN METABOLISM, GLUTAMATE BIOSYNTHESIS. THE CATALYZED REACTION BRINGS TOGETHER THE NITROGEN AND CARBON METABOLISM. [C]

COG id: COG0493

COG function: function code ER; NADPH-dependent glutamate synthase beta chain and related oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 4Fe-4S ferredoxin-type domain [H]

Homologues:

Organism=Homo sapiens, GI119943098, Length=479, Percent_Identity=28.6012526096033, Blast_Score=162, Evalue=5e-40,
Organism=Escherichia coli, GI1789606, Length=472, Percent_Identity=99.364406779661, Blast_Score=969, Evalue=0.0,
Organism=Escherichia coli, GI1788811, Length=461, Percent_Identity=66.8112798264642, Blast_Score=650, Evalue=0.0,
Organism=Escherichia coli, GI87082180, Length=450, Percent_Identity=59.7777777777778, Blast_Score=562, Evalue=1e-161,
Organism=Escherichia coli, GI1788468, Length=442, Percent_Identity=31.6742081447964, Blast_Score=186, Evalue=2e-48,
Organism=Escherichia coli, GI1789243, Length=425, Percent_Identity=31.5294117647059, Blast_Score=154, Evalue=1e-38,
Organism=Caenorhabditis elegans, GI17570289, Length=496, Percent_Identity=33.4677419354839, Blast_Score=223, Evalue=1e-58,
Organism=Caenorhabditis elegans, GI71984108, Length=466, Percent_Identity=30.4721030042918, Blast_Score=161, Evalue=7e-40,
Organism=Caenorhabditis elegans, GI17543860, Length=175, Percent_Identity=30.2857142857143, Blast_Score=73, Evalue=4e-13,
Organism=Saccharomyces cerevisiae, GI6320030, Length=469, Percent_Identity=33.4754797441365, Blast_Score=232, Evalue=9e-62,
Organism=Drosophila melanogaster, GI28574881, Length=485, Percent_Identity=39.7938144329897, Blast_Score=299, Evalue=2e-81,
Organism=Drosophila melanogaster, GI24665539, Length=485, Percent_Identity=39.7938144329897, Blast_Score=299, Evalue=2e-81,
Organism=Drosophila melanogaster, GI24665547, Length=485, Percent_Identity=39.7938144329897, Blast_Score=298, Evalue=4e-81,
Organism=Drosophila melanogaster, GI24665543, Length=485, Percent_Identity=39.7938144329897, Blast_Score=298, Evalue=4e-81,
Organism=Drosophila melanogaster, GI24640763, Length=460, Percent_Identity=27.8260869565217, Blast_Score=157, Evalue=2e-38,
Organism=Drosophila melanogaster, GI18858217, Length=460, Percent_Identity=27.8260869565217, Blast_Score=157, Evalue=2e-38,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR013027
- InterPro:   IPR012285
- InterPro:   IPR006006
- InterPro:   IPR009051
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: =1.4.1.13 [H]

Molecular weight: Translated: 52030; Mature: 51899

Theoretical pI: Translated: 5.43; Mature: 5.43

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQADRCLSCGNPYCEWKCP
CCCCHHHHHHHCCCCCCCCCCEEEEEEEEEEECCHHCCHHHHHHHHHHHCCCCCEEEECC
VHNYIPNWLKLANEGRIFEAAELSHQTNTLPEVCGRVCPQDRLCEGSCTLNDEFGAVTIG
HHHHCCHHHHHCCCCCEEEHHHHCCCCCCHHHHHHHHCCCCCCCCCCCEECCCCCEEEEC
NIERYINDKAFEMGWRPDMSGVKQTGKKVAIIGAGPAGLACADVLTRNGVKAVVFDRHPE
CHHHHHCCHHHCCCCCCCHHHHHHCCCEEEEEECCCCHHHHHHHHHCCCCEEEEEECCCC
IGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTEVGRDVQLDDLLSDYDAVFLGVG
CCCEEEECCCHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHCCCEEEEECC
TYQSMRGGLENEDADGVYAALPFLIANTKQLMGFGETREEPFVSMEGKRVVVLGGGDTAM
CHHHHHCCCCCCCCCCHHHHHHHHHHCCHHHHCCCCCCCCCCEEECCCEEEEECCCCHHH
DCVRTSVRQGAKHVTCAYRRDEENMPGSRREVKNAREEGVEFKFNVQPLGIEVNGNGKVS
HHHHHHHHCCCCEEEEEEECCCCCCCCHHHHHHHHHHCCCEEEEEEEEEEEEECCCCCCC
GVKMVRTEMGEPDAKGRRRAEIVAGSEHIVPADAVIMAFGFRPHSMEWLAKHSVELDSQG
CEEEEEHHCCCCCCCCCCEEEEEECCCCCCCCCCEEEEECCCCCCHHHHHHCCCCCCCCC
RIIAPEGNDNAFQTSNPKIFAGGDIVRGSDLVVTAIAEGRKAADGIMNWLEV
CEECCCCCCCCEECCCCEEEECCCEEECCCEEEEEECCCCHHHHHHHHHHCC
>Mature Secondary Structure 
SQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQADRCLSCGNPYCEWKCP
CCCHHHHHHHCCCCCCCCCCEEEEEEEEEEECCHHCCHHHHHHHHHHHCCCCCEEEECC
VHNYIPNWLKLANEGRIFEAAELSHQTNTLPEVCGRVCPQDRLCEGSCTLNDEFGAVTIG
HHHHCCHHHHHCCCCCEEEHHHHCCCCCCHHHHHHHHCCCCCCCCCCCEECCCCCEEEEC
NIERYINDKAFEMGWRPDMSGVKQTGKKVAIIGAGPAGLACADVLTRNGVKAVVFDRHPE
CHHHHHCCHHHCCCCCCCHHHHHHCCCEEEEEECCCCHHHHHHHHHCCCCEEEEEECCCC
IGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTEVGRDVQLDDLLSDYDAVFLGVG
CCCEEEECCCHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHCCCEEEEECC
TYQSMRGGLENEDADGVYAALPFLIANTKQLMGFGETREEPFVSMEGKRVVVLGGGDTAM
CHHHHHCCCCCCCCCCHHHHHHHHHHCCHHHHCCCCCCCCCCEEECCCEEEEECCCCHHH
DCVRTSVRQGAKHVTCAYRRDEENMPGSRREVKNAREEGVEFKFNVQPLGIEVNGNGKVS
HHHHHHHHCCCCEEEEEEECCCCCCCCHHHHHHHHHHCCCEEEEEEEEEEEEECCCCCCC
GVKMVRTEMGEPDAKGRRRAEIVAGSEHIVPADAVIMAFGFRPHSMEWLAKHSVELDSQG
CEEEEEHHCCCCCCCCCCEEEEEECCCCCCCCCCEEEEECCCCCCHHHHHHCCCCCCCCC
RIIAPEGNDNAFQTSNPKIFAGGDIVRGSDLVVTAIAEGRKAADGIMNWLEV
CEECCCCCCCCEECCCCEEEECCCEEECCCEEEEEECCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3326786; 9278503; 9298646; 9600841; 2643092 [H]