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The map label for this gene is agaI

Identifier: 218701916

GI number: 218701916

Start: 3801419

End: 3802186

Strand: Direct

Name: agaI

Synonym: ECIAI39_3642

Alternate gene names: 218701916

Gene position: 3801419-3802186 (Clockwise)

Preceding gene: 218701915

Following gene: 218701918

Centisome position: 74.07

GC content: 50.52

Gene sequence:

>768_bases
ATGGAACGAGGCACTGCGTCTGGCGGTGCCTCTTTACTTAAGGAATTTCATCCTGTGCAAACCCTTCAACAAGTTGAAAA
CTATACGGCGTTAAGTGAACGTGCCAGCGAATATTTATTGGCCGTGATCCGTAGTAAACCGGATGCCGTGATTTGCCTGG
CGACCGGAGCCACGCCATTACTGACGTATCATTATCTGGTAGAAAAAATCCACCAGCAGCAGGTTGATGTCAGCCAGCTC
ACCTTCGTGAAGCTCGACGAATGGGTGGATCTGCCATTAACAATGCCTGGCACCTGCGAAACTTTCCTACAACAGCATAT
CGTGCAGCCGCTGGGGCTGCGTGAAGACCAGCTCATCAGCTTTCGCTCTGAAGAGATAAATGAGACAGAGTGCGAACGGG
TAACGAACCTGATTGCGCGCAAAGGCGGTCTGGATTTATGCGTTCTCGGATTGGGGAAAAACGGTCATCTTGGGCTGAAC
GAACCGGGAGAAAGCCTGCAACCGGCCTGCCATATCAGTCAACTTGATGCCAGAACACAGCAACATGAGATGTTAAAAAC
CGCGGGCCGTCCCGTGACTCGTGGGATCACCTTAGGCCTGAAGGATATTCTCAATACCCGCGAAGTTTTGCTACTGGTGA
CTGGCGAAGGAAAGCAGGATGCGACAGAACGTTTTCTCACGGCTAAAGTCTCTACCGCTATCCCGGCTTCGTTTTTATGG
CTGCATAACCATTTCACATGCGTGATTGATGAAATGTGTCGTCGTTAA

Upstream 100 bases:

>100_bases
GATGTATTACTTCCTGCGGGTGAAAAAAGCGCATCCGGTGCTGTTAATCGGCGTGACTTTTGTGCTCTCTATTGTTTGTT
CCGCATTCGGCATTTTGTAA

Downstream 100 bases:

>100_bases
CTCGCCTGGTAAAGGCGTTGTAGCGCTATTCCTGCTGCAACGCCTGTAATCACTCTCCCTGCTGCTCCAGTGCATACTTA
TACAGCGCATTTTTCTTCAC

Product: galactosamine-6-phosphate isomerase

Products: D-Tagatose 6-Phosphate; NH3. [C]

Alternate protein names: Galactosamine-6-phosphate deaminase [H]

Number of amino acids: Translated: 255; Mature: 255

Protein sequence:

>255_residues
MERGTASGGASLLKEFHPVQTLQQVENYTALSERASEYLLAVIRSKPDAVICLATGATPLLTYHYLVEKIHQQQVDVSQL
TFVKLDEWVDLPLTMPGTCETFLQQHIVQPLGLREDQLISFRSEEINETECERVTNLIARKGGLDLCVLGLGKNGHLGLN
EPGESLQPACHISQLDARTQQHEMLKTAGRPVTRGITLGLKDILNTREVLLLVTGEGKQDATERFLTAKVSTAIPASFLW
LHNHFTCVIDEMCRR

Sequences:

>Translated_255_residues
MERGTASGGASLLKEFHPVQTLQQVENYTALSERASEYLLAVIRSKPDAVICLATGATPLLTYHYLVEKIHQQQVDVSQL
TFVKLDEWVDLPLTMPGTCETFLQQHIVQPLGLREDQLISFRSEEINETECERVTNLIARKGGLDLCVLGLGKNGHLGLN
EPGESLQPACHISQLDARTQQHEMLKTAGRPVTRGITLGLKDILNTREVLLLVTGEGKQDATERFLTAKVSTAIPASFLW
LHNHFTCVIDEMCRR
>Mature_255_residues
MERGTASGGASLLKEFHPVQTLQQVENYTALSERASEYLLAVIRSKPDAVICLATGATPLLTYHYLVEKIHQQQVDVSQL
TFVKLDEWVDLPLTMPGTCETFLQQHIVQPLGLREDQLISFRSEEINETECERVTNLIARKGGLDLCVLGLGKNGHLGLN
EPGESLQPACHISQLDARTQQHEMLKTAGRPVTRGITLGLKDILNTREVLLLVTGEGKQDATERFLTAKVSTAIPASFLW
LHNHFTCVIDEMCRR

Specific function: N-acetylgalactosamine utilization. [C]

COG id: COG0363

COG function: function code G; 6-phosphogluconolactonase/Glucosamine-6-phosphate isomerase/deaminase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glucosamine/galactosamine-6-phosphate isomerase family [H]

Homologues:

Organism=Homo sapiens, GI19923881, Length=237, Percent_Identity=26.5822784810127, Blast_Score=89, Evalue=4e-18,
Organism=Homo sapiens, GI13027378, Length=240, Percent_Identity=26.25, Blast_Score=89, Evalue=6e-18,
Organism=Escherichia coli, GI1789530, Length=250, Percent_Identity=96.8, Blast_Score=499, Evalue=1e-143,
Organism=Escherichia coli, GI1786893, Length=244, Percent_Identity=27.8688524590164, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI48994958, Length=227, Percent_Identity=26.8722466960352, Blast_Score=73, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17554876, Length=234, Percent_Identity=28.2051282051282, Blast_Score=100, Evalue=7e-22,
Organism=Drosophila melanogaster, GI24581960, Length=240, Percent_Identity=24.1666666666667, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI19920764, Length=240, Percent_Identity=24.1666666666667, Blast_Score=77, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006148
- InterPro:   IPR004547
- InterPro:   IPR018321 [H]

Pfam domain/function: PF01182 Glucosamine_iso [H]

EC number: 5.3.1.- [C]

Molecular weight: Translated: 28369; Mature: 28369

Theoretical pI: Translated: 6.03; Mature: 6.03

Prosite motif: PS01161 GLC_GALNAC_ISOMERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MERGTASGGASLLKEFHPVQTLQQVENYTALSERASEYLLAVIRSKPDAVICLATGATPL
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHH
LTYHYLVEKIHQQQVDVSQLTFVKLDEWVDLPLTMPGTCETFLQQHIVQPLGLREDQLIS
HHHHHHHHHHHHHHCCHHHHHEEEHHCCCCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHH
FRSEEINETECERVTNLIARKGGLDLCVLGLGKNGHLGLNEPGESLQPACHISQLDARTQ
HHHHCCCHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
QHEMLKTAGRPVTRGITLGLKDILNTREVLLLVTGEGKQDATERFLTAKVSTAIPASFLW
HHHHHHHCCCCHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHHHCCHHHHH
LHNHFTCVIDEMCRR
HHCCHHHHHHHHHCC
>Mature Secondary Structure
MERGTASGGASLLKEFHPVQTLQQVENYTALSERASEYLLAVIRSKPDAVICLATGATPL
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHH
LTYHYLVEKIHQQQVDVSQLTFVKLDEWVDLPLTMPGTCETFLQQHIVQPLGLREDQLIS
HHHHHHHHHHHHHHCCHHHHHEEEHHCCCCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHH
FRSEEINETECERVTNLIARKGGLDLCVLGLGKNGHLGLNEPGESLQPACHISQLDARTQ
HHHHCCCHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
QHEMLKTAGRPVTRGITLGLKDILNTREVLLLVTGEGKQDATERFLTAKVSTAIPASFLW
HHHHHHHCCCCHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHHHCCHHHHH
LHNHFTCVIDEMCRR
HHCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: D-Galactosamine 6-Phosphate; H2O [C]

Specific reaction: D-Galactosamine 6-Phosphate + H2O = D-Tagatose 6-Phosphate + NH3. [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10931310; 9278503; 8932697 [H]